G
DAD
DE
LAS
PALMAS
DE
GRAN
CANARIA
Bioges
' ·
...
o
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..
....,,'-....i_
.....
,.,..¡,,."
..
Jl
1
l:
..
iIm""' .W,,,
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1
Respi a o y me abolism
in
mac oalgae: Using
he
espi a o y elec on
anspo
IDeAO
sys em (ETS)
o
de ec
s ess and
di e en
physiological s a es.
~
~
M.T.
Asenslo1
,
F.
Maldonado1,
M.
Gómez
1, T. T.
Packa d
1 and M.A. Vle a-Rod iguez2
bi<D>cean
I
G upo
de
Eco isiologla
Planc 6nica,
Ins i u o
de
Oceanog a l'a
y cambio Gl
obal
.
Un
i
e sidad
de
Las
Pal
m
as
de
G a
n cana
i
a,
Sp
aln.
SQauho
n s
In oduc ion
Me abollsm s udy
o
g een algae
(Ul a)
communi les, Inhabi lng In e Udal pools
o
G an
cana ia.
As
an
Index
o
me abollc s a us
and
s ess
we
used
Ile elec on
nmspo
sys em
(ETS)
o
dl e en la e be ween di e en g owlng condi lons
In
Ile nll ul8l
en l onmen .
lhls
echnlque
has
been
success ully
used
o
s udy many di e en ma ine
plank onlc o ganlsms Indudlng bac e ia, phy oplank on and zooplank on,
bu
i
has
no
been
used
o
s udy ma ine mac oalgae.
In
IlIs l s phase
o
ou esea ch we ha e de eloped Ile me hodology
o
homogenlzlng
U/ a
and
ha e
used
a s anda d spec opho omeb1c-based klne ic
enzyme
assay
o
desc ibe Ile Impac
o
nu len IImi a lon
on
Ile me abollc capaci y
In
sampes
collec ed
In
Ile wild
and
maln alned
In
con olled cul u e.
1.
Choice
o
he
homogenlzlon
me hod:
The
di e ence be ween bo ll me hods was slgnl lcan ,
In
addi lon, we declded
o
use
Ile lssue-g lndlng me hod
because i was less expensl e and easle .
2.
Co ela lon
be ween
dl '
..
en
blomass
p oxles.
lhe e
15
a
goo<l
co ela lon
be ween d y welgh , op ical densi y
and
chlo ophyll a & b (Ags
S,
6, & 7.
.-..
0,9
~
0,_
2
0,7
..,
o,e
0,5
0,4
0,3
i§
0,2
¡
O,!
+.
O
20
40
O y
welgh
(mg)
30
25
1
20
'O
15
-
~10
6 5
O
60
.
-;
+
..
..
.
.
-
O
20
40
O y
wlegh
(mg)
+ .
..
60
....
~I
Figu e 1: M
ap
o
(¡a
n
cana ia.
We
co
U
ec ed
samples
a
Sa
n
Oi
s obal
and
Ta
H
a e
(Te
l
de
).
0,015
..--
-
--------
--
-
--...,
i
0,04
B 0,
03
!
0,02
~
0,01
0,00
"------
_
G inde
-
L1quld
Ni ogen
igu e
4:
Compa ison
be ween
ETS
o al in
bo h
homogeniza ion
me hods.
• Chl a
• Chl b
30
Z5
{zo
:!!.15
...
.!:
10
6 5 .
..
. .
• •
O ¡
. '
~
.
o
0,2
0,4 0,6 O
,.
Op laol
deNl¡ly
(670
nm
)
+
Ch
lll
-Ch b
Mean
0>1
3.
E olu on
o
ETS
wl h
ime
4.
Rela lon
be ween
Blomass
and
Respl a o y
ac l l y
(Klelbe )
i'
1,2
1,1
1,0
~
0,11
0,_
!
0,7
E
0,6
I
0,5
0,4
O Z
_
10
We
collec ed
lile
algae
(day
1)
and
s o ed
lIlem a 200c
o 8
days
wi hou nu ien s
wi h a
10-14
pho ope iod.
In
spi e
o lile appa en
di e ences
be ween
Ihe
wo
a eas
hey
we e
no
s a is ically di e en
because
o lile
la ge
s anda d
de ia ion
in
lile
San
C is obal
da a
al e 5 h
day
.
(We
used
PERMANOVA
.1.6
so wa e
(M.J.
Ande son,
2(05).
Figu e 8:
ETS
ime-cou se
in
Ioca ions.
e encea
Kenne
and
Ahmed,
19
75.
Masu emen s
o
Elec on
T anspo
Ac i
i
ies
in
ma ine
Phy oplank on
.
Ma i
i
ne
Biology
33,
119-127.
Mi chell
and
Kie e
,
1984
. De e mina ion o
abso p ion
and
luo escence
exci a ion
spec a
o phy oplank on.
Lec u e
no es
on
cos al
and
es ua ines
s udies,
VOL
8,
PP
.
157
-
169
.
Ande son,
M.J.
2005
.
PERMANOVA
: a
FORTRAN
compu e
p og am
o
penmu a ional
mul i a ia e
analysis
o
a iance
.
Depa men
o
S a is ics,
Uni e si y
T, y •
0,5211x
- 2,
3324
y • 0,
35+1><
-
2,
1173
R'·O,5664
R' · 0,
419
T. y •
0,61S31
-
2, 1J2
y • 0,
1S82x
• 2p m
R
:l.
O,7828
R' • 0,
1316
T. y • .o,26Jk • 1p7S7 y •
0,1688x
·
2P' l
R'.Op391
R' ·
Op593
T.bIe 1:
The
Kleibe
coe iden
clec eases
om
Ihe l s o Ihe
las
day
due
o
s a a ion.
1.
compa son
be ween ETS
melIns
In
bo h
homogenlza lon
me hods
demolls la ed a slgnl lam dll e ence.
We
used Ile lssue-g 1ndlng me hod
because
I
was
Iess
expensI e
and
easIe .
2. The e
Is
a
good
co eIaaon
be ween d y welgh , op Ical densi y and
Chlo ophyll
10
we used he op IaIl
cIensI y
a 670 nm
as
a e e ence
o
meau e
bIomass.
3. The di e ences
In
he
8-cIay
ETS
ime cou ses o he
wo
a eas
we e
no
s a Is IaIlly dl e en .
4.
Due
Ile s 3 ed condl lons we could
see
a dec ease
In
he Klelbe