Respiratory metabolism in macroalgae: Using the respiratory electron transport system (ETS) to detect stress and different physiological states

G DAD DE LAS PALMAS DE GRAN CANARIA Bioges ' · ... o ..... I .. ....,,'-....i_ ..... ,.,..¡,,." .. Jl 1 l: .. iIm""' .W,,, .. 1 Respi a o y me abolism in mac oalgae: Using he espi a o y elec on anspo IDeAO sys em (ETS) o de ec s ess and di e en physiological s a es. ~ ~ M.T. Asenslo1 , F. Maldonado1, M. Gómez 1, T. T. Packa d 1 and M.A. Vle a-Rod iguez2 bi<D>cean I G upo de Eco isiologla Planc 6nica, Ins i u o de Oceanog a l'a y cambio Gl obal . Un i e sidad de Las Pal m as de G a n cana i a, Sp aln. SQauho n s In oduc ion Me abollsm s udy o g een algae (Ul a) communi les, Inhabi lng In e Udal pools o G an cana ia. As an Index o me abollc s a us and s ess we used Ile elec on nmspo sys em (ETS) o dl e en la e be ween di e en g owlng condi lons In Ile nll ul8l en l onmen . lhls echnlque has been success ully used o s udy many di e en ma ine plank onlc o ganlsms Indudlng bac e ia, phy oplank on and zooplank on, bu i has no been used o s udy ma ine mac oalgae. In IlIs l s phase o ou esea ch we ha e de eloped Ile me hodology o homogenlzlng U/ a and ha e used a s anda d spec opho omeb1c-based klne ic enzyme assay o desc ibe Ile Impac o nu len IImi a lon on Ile me abollc capaci y In sampes collec ed In Ile wild and maln alned In con olled cul u e. 1. Choice o he homogenlzlon me hod: The di e ence be ween bo ll me hods was slgnl lcan , In addi lon, we declded o use Ile lssue-g lndlng me hod because i was less expensl e and easle . 2. Co ela lon be ween dl ' .. en blomass p oxles. lhe e 15 a goo<l co ela lon be ween d y welgh , op ical densi y and chlo ophyll a & b (Ags S, 6, & 7. .-.. 0,9 ~ 0,_ 2 0,7 .., o,e 0,5 0,4 0,3 i§ 0,2 ¡ O,! +. O 20 40 O y welgh (mg) 30 25 1 20 'O 15 - ~10 6 5 O 60 . -; + .. .. . . - O 20 40 O y wlegh (mg) + . .. 60 .... ~I Figu e 1: M ap o (¡a n cana ia. We co U ec ed samples a Sa n Oi s obal and Ta H a e (Te l de ). 0,015 ..-- - -------- -- - --..., i 0,04 B 0, 03 ! 0,02 ~ 0,01 0,00 "------ _ G inde - L1quld Ni ogen igu e 4: Compa ison be ween ETS o al in bo h homogeniza ion me hods. • Chl a • Chl b 30 Z5 {zo :!!.15 ... .!: 10 6 5 . .. . . • • O ¡ . ' ~ . o 0,2 0,4 0,6 O ,. Op laol deNl¡ly (670 nm ) + Ch lll -Ch b Mean 0>1 3. E olu on o ETS wl h ime 4. Rela lon be ween Blomass and Respl a o y ac l l y (Klelbe ) i' 1,2 1,1 1,0 ~ 0,11 0,_ ! 0,7 E 0,6 I 0,5 0,4 O Z _ 10 We collec ed lile algae (day 1) and s o ed lIlem a 200c o 8 days wi hou nu ien s wi h a 10-14 pho ope iod. In spi e o lile appa en di e ences be ween Ihe wo a eas hey we e no s a is ically di e en because o lile la ge s anda d de ia ion in lile San C is obal da a al e 5 h day . (We used PERMANOVA .1.6 so wa e (M.J. Ande son, 2(05). Figu e 8: ETS ime-cou se in Ioca ions. e encea Kenne and Ahmed, 19 75. Masu emen s o Elec on T anspo Ac i i ies in ma ine Phy oplank on . Ma i i ne Biology 33, 119-127. Mi chell and Kie e , 1984 . De e mina ion o abso p ion and luo escence exci a ion spec a o phy oplank on. Lec u e no es on cos al and es ua ines s udies, VOL 8, PP . 157 - 169 . Ande son, M.J. 2005 . PERMANOVA : a FORTRAN compu e p og am o penmu a ional mul i a ia e analysis o a iance . Depa men o S a is ics, Uni e si y T, y • 0,5211x - 2, 3324 y • 0, 35+1>< - 2, 1173 R'·O,5664 R' · 0, 419 T. y • 0,61S31 - 2, 1J2 y • 0, 1S82x • 2p m R :l. O,7828 R' • 0, 1316 T. y • .o,26Jk • 1p7S7 y • 0,1688x · 2P' l R'.Op391 R' · Op593 T.bIe 1: The Kleibe coe iden clec eases om Ihe l s o Ihe las day due o s a a ion. 1. compa son be ween ETS melIns In bo h homogenlza lon me hods demolls la ed a slgnl lam dll e ence. We used Ile lssue-g 1ndlng me hod because I was Iess expensI e and easIe . 2. The e Is a good co eIaaon be ween d y welgh , op Ical densi y and Chlo ophyll 10 we used he op IaIl cIensI y a 670 nm as a e e ence o meau e bIomass. 3. The di e ences In he 8-cIay ETS ime cou ses o he wo a eas we e no s a Is IaIlly dl e en . 4. Due Ile s 3 ed condl lons we could see a dec ease In he Klelbe