Me agenomic Sequence o P oka yo ic Mic obio a om an
In e media e-Salini y Pond o a Sal e n in Isla C is ina, Spain
Ana B. Fe nández, Ma ía José León, Blanca Ve a, C is ina Sánchez-Po o, An onio Ven osa
Depa men o Mic obiology and Pa asi ology, Facul y o Pha macy, Uni e si y o Se ille, Se ille, Spain
Ma ine sal e ns a e a i icial mul ipond sys ems designed o he comme cial p oduc ion o sal by e apo a ion o seawa e . We
epo he e he me agenomic sequence o he p oka yo ic mic obio a o a pond wi h in e media e salini y (21% o al sal s) o a
sal e n loca ed in Isla C is ina, Huel a, sou hwes Spain.
Recei ed 15 Janua y 2014 Accep ed 23 Janua y 2014 Published 13 Feb ua y 2014
Ci a ion Fe nández AB, León MJ, Ve a B, Sánchez-Po o C, Ven osa A. 2014. Me agenomic sequence o p oka yo ic mic obio a om an in e media e-salini y pond o a sal e n
in Isla C is ina, Spain. Genome Announc. 2(1):e00045-14. doi:10.1128/genomeA.00045-14.
Copy igh © 2014 Fe nández e al. This is an open-access a icle dis ibu ed unde he e ms o he C ea i e Commons A ibu ion 3.0 Unpo ed license.
Add ess co espondence o An onio Ven osa, [email p o ec ed].
Ma ine sal e ns a e composed o in e connec ed ponds wi h
inc easing sal concen a ions and hey cons i u e excellen
models o s udying mic obial popula ions g owing along a salini-
y g adien (1). They a e used o he comme cial p oduc ion o
sal by e apo a ion o seawa e (2). Mic obiological s udies o
hese ex eme habi a s ha e enabled iden i ica ion o he mic oo -
ganisms inhabi ing hem (3–5). Howe e , mos s udies ha e been
ocused on he mos concen a ed hype saline ponds, designa ed
c ys allize s, in which he NaCl is p ecipi a ed (6–8). Molecula
s udies ha e shown ha he wo main p oka yo es which a e p e-
dominan in hese NaCl-sa u a ed ponds a e he haloa chaeon
Haloquad a um walsbyi and he bac e oide e Salinibac e ube (9,
10). Recen me agenomic s udies ca ied ou in he sal e ns in
San a Pola, eas Spain, ha e con i med hese indings (11). How-
e e , in con as o he indings o educed mic obial di e si y in
he highly saline ponds (c ys allize s), analysis o he me age-
nomic da a se o an in e media e-salini y pond wi h 19% sal s
indica ed ha a much wide p oka yo ic di e si y han expec ed
was p esen (11). In his epo , we desc ibe he sequencing o a
p oka yo ic me agenome ob ained om a pond o in e media e
salini y o a ma ine sal e n loca ed in Isla C is ina, Huel a, sou h-
wes Spain, on he A lan ic Ocean, a away om he San a Pola
sal e n, which is on he Medi e anean coas .
A 50-li e wa e sample was ob ained om he su ace o a
pond wi h 21% o al sal s (Global Posi ioning Sys em [GPS] co-
o dina es 37°13=08⬙N, 7°20=17⬙W) on July 2010. The sample was
sequen ially il e ed h ough 5.0- and 0.22-
␮
m-po e size polyca -
bona e il e s (Millipo e) as p e iously desc ibed (11). To al en i-
onmen al DNA was ex ac ed and pu i ied ollowing he p o o-
cols desc ibed elsewhe e (11,12). The pu i ied p oka yo ic DNA
was sequenced by py osequencing (Roche 454 GS-FLX sys em,
Ti anium chemis y) by GATC, Cons ance, Ge many. A o al o
1,223,923 eads wi h an a e age ead leng h o 397 bp we e ob-
ained. The size o he me agenomic da a se was 486,317,437 bp.
Sequences ela ed o 16S ibosomal RNA genes we e iden i ied
using he Ribosomal Da abase P ojec (RDP) (13). In addi ion,
sequences we e compa ed agains he MG-RAST se e (14),
Communi y Cybe in as uc u e o Ad anced Mic obial Ecology
Resea ch and Analysis (CAMERA) (15), and In eg a ed Mic obial
Genomes wi h Mic obiome Samples-Expe Re iew (IMG/MER)
(16) da abases.
Eu ya chaeo a (~84%) was he mos abundan phylum p esen in
his sample, ollowed by Bac e oide es (~8%) and Gammap o eobac-
e ia (~7%), sugges ing a dec ease o he mic obial di e si y in sal
pondso sal e nswhen hesalini y eachessal concen a ions⬎20%,
being mo e simila o hose ound in c ys allize ponds han hose
ound in in e media e-salini y ponds wi h ⬍20% sal s.
Fu he me agenome analysis will con ibu e inc eased unde -
s anding o he gene ic di e si y p esen in ma ine sal e ns and
pa icula ly in ponds wi h in e media e salini ies whe e he en i-
onmen al condi ions limi he p esence o halophilic bac e ia and
enhance he g ow h o haloa chaea.
Nucleo ide sequence accession numbe . The sequences ob-
ained in his p ojec ha e been deposi ed in he NCBI Sequence
Read A chi e unde he accession no. SRP029970.
ACKNOWLEDGMENTS
This esea ch was suppo ed by g an s om he Spanish Minis y o Sci-
ence and Inno a ion (CGL2010-19303) and he Jun a de Andalucía (P10-
CVI-6226). FEDER unds and he Plan Andaluz de In es igación also
suppo ed his p ojec . Ana Bea iz Fe nández and Ma ía José León we e
suppo ed by pos doc o al and pos g adua e g an s, espec i ely, om he
Jun a de Andalucía.
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Fe nández e al.
Genome Announcemen s2genomea.asm.o g Janua y/Feb ua y 2014 Volume 2 Issue 1 e00045-14