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Snap Shut Slide Imaging Chamber V1

Kurtz, Ryan

Abstract

The Snap Shut Slide Imaging Chamber V1 is a 3D-printable, reusable chamber designed for stable mounting and live imaging of zebrafish (Danio rerio) embryos under a microscope. The chamber has the same outer dimensions as a standard glass slide and can be viewed from either side, allowing consistent sample positioning during optical imaging. The design was inspired by the viewing chamber constructed from standard glass cover slips described in The Zebrafish Book (Westerfield, 2000). The 3D-printed housing secures the viewing chamber with a snap-fit mechanism, enabling rapid sample preparation, compatibility with multiple microscope types and sample orientations, improved reproducibility, and support for open-hardware practices in developmental biology research.

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Title: Snap Shut Slide Imaging Chamber V1 Author/Designer: Ryan Kurtz, Amherst College Date: September 2025 This 3D model is released under the Creative Commons Attribution–NonCommercial 4.0 International License (CC BY-NC 4.0). Licensed under CC BY-NC 4.0 (https://creativecommons.org/licenses/by-nc/4.0/). You are free to: - Share — copy and redistribute the material in any medium or format - Adapt — remix, transform, and build upon the material Under the following terms: - Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. - NonCommercial — You may not use the material for commercial purposes. Required attribution: “This design, Snap Shut Slide Imaging Chamber V1, was created by Ryan Kurtz (2025) and is used under the CC BY-NC 4.0 license.” If used in a publication, please cite as: Kurtz, R. (2025, October). Snap Shut Slide Imaging Chamber V1 [3D model]. Amherst College. Zenodo. https://doi.org/10.5281/zenodo.17244895 No warranties are provided. Use at your own risk. Happy imaging. Inspiration for this work came from: Bradford, Y.M., Van Slyke, C.E., Ruzicka, L., Singer, A., Eagle, A., Fashena, D., Howe, D.G., Frazer, K., Martin, R., Paddock, H., Pich, C., Ramachandran, S., Westerfield, M. (2022) Zebrafish Information Network, the knowledgebase for Danio rerio research. Genetics. 220(4). PMID: 35166825 PMCID: PMC8982015 DOI: 10.1093/genetics/iyac016