DETERMINATION OF WATER-SOLUBLE VITAMINS IN THE ABOVE-GROUND PART OF THE CREEPING CLOVER

233 Volume 5, Issue 10: Special Issue (EJAR) ISSN: 2181-2020 MPHAPP THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “MODERN PHARMACEUTICS: ACTUAL PROBLEMS AND PROSPECTS” TASHKENT, OCTOBER 17, 2025 in-academy.uz DETERMINATION OF WATER-SOLUBLE VITAMINS IN THE ABOVE-GROUND PART OF THE CREEPING CLOVER Abduganieva M.F.1 Xalilova Sh.R.1 Urmanova F.F.1 Sayakova G.M.2 1Tashkent pharmaceutical institute, Tashkent city, Republic of Uzbekistan 2Asfendiyarov Kazakh National Medical University, Almaty city, Republic of Kazakhstan e-mail: [email protected] https://doi.org/10.5281/zenodo.17333391 Relevance. it is well known that vitamins play a role in energy metabolism, the biosynthesis of amino acids and fatty acids, photorepression processes, blood coagulation, calcium absorption, and more. Among the water-soluble vitamins, the B-group vitamins (B1, B2, B5, B6, B9/folate, B12) and vitamin C are excreted in urine if consumed in excess, as they are not stored in the body. Therefore, their daily intake is essential. These vitamins play an important role in strengthening the immune system, ensuring proper energy production, and protecting the body from various diseases. The aim of the study: to determine the content of water-soluble vitamins in the herb of the creeping plant Trifolium repens L. (white clover) using high-performance liquid chromatography (HPLC). Materials and methods: the aerial parts of the creeping plant Trifolium repens L. were collected in Tashkent city in 2025 during the full flowering phase and used as the object of study. For the determination of water-soluble vitamins using high-performance liquid chromatography (HPLC), acetate buffer systems and acetonitrile were used as eluents (Figure 1). Chromatographic conditions: HPLC system: Agilent 1200, equipped with an autosampler; Column: Eclipse XDBC18, 5 µm, 4.6 × 250 mm; Detector: Diode Array Detector (DAD), detection at 250 nm; Flow rate: 0.8 mL/min; Eluent composition (acetate buffer : acetonitrile): 0–5 min → 96:4; 6–8 min → 90:10; 9–15 min → 80:20; 15–17 min → 96:4; Column oven temperature: 25°C. Figure 1. Chromatogram of water-soluble vitamins in the aerial part of Trifolium repens L. Results: the quantitative content of water-soluble vitamins in the aerial part of Trifolium repens L., as determined by HPLC, is summarized in Table 1. Table 1 № Vitamins Retention time Quantity, mg/gr 1. С (ascorbic acid) 5,356 59,3 2. В3 (nikotinic acid) 5,979 2,54 3. B1 (thiamine) 9,670 25,6 234 Volume 5, Issue 10: Special Issue (EJAR) ISSN: 2181-2020 MPHAPP THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “MODERN PHARMACEUTICS: ACTUAL PROBLEMS AND PROSPECTS” TASHKENT, OCTOBER 17, 2025 in-academy.uz The Content of Water-Soluble Vitamins in the Aerial Part of Trifolium repens L. Conclusions: HPLC analysis of the aerial part of Trifolium repens L. identified ascorbic acid (vitamin C) as the predominant water-soluble vitamin, supporting the plant’s chemical characterization. 4. B6 (pyridoxine) 11,657 37,2 5. B9 (folic acid) 14,046 42,4 6. B2 (riboflavin) 15,012 39,4