DETERMINATION OF THE AUTHENTICITY OF THE GLABRUS DRY EXTRACT

251 Volume 5, Issue 10: Special Issue (EJAR) ISSN: 2181-2020 MPHAPP THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “MODERN PHARMACEUTICS: ACTUAL PROBLEMS AND PROSPECTS” TASHKENT, OCTOBER 17, 2025 in-academy.uz DETERMINATION OF THE AUTHENTICITY OF THE GLABRUS DRY EXTRACT Karayeva N.Y.¹ Turdiyeva Z.V.¹ Abdullajonova N.G‘.² ¹ Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan ²Institute of Bioorganic Chemistry named after A.S. Sodiqov, Tashkent city, Republic of Uzbekistan e-mail: [email protected] https://doi.org/10.5281/zenodo.17333630 Relevance: according to literature data, about 250 species of the plant Rhus belonging to the family Anacardiaceae are known and occur on almost every continent. Both the world scientific community and researchers in our country are studying the composition of Rhus species and exploring their practical applications. The smooth sumac (Rhus glabra), a member of this family, was used in folk medicine by Avicenna (Abu Ali ibn Sino). Preparations made from this plant were applied in the treatment of intestinal ulcers, ear pain, facial nerve paralysis, to enhance the function of the digestive organs, for diarrhea, to stop bleeding, for diabetes, and to treat various other ailments. Purpose of the study: the main objective of our research was to determine the authenticity of the conditionally named “Glabrus” dry extract obtained from the smooth sumac (Rhus glabra) plant using the thin-layer chromatography (TLC) method. Materials and Methods: the study was carried out in accordance with Section 2.2.27 (TLC method) of the State Pharmacopoeia of the Republic of Uzbekistan, Vol. I, Part 1. First, 100 mg of the working standard sample was dissolved in 10 ml of a methanol–water (70:30) mixture and filtered. The filtrate was used as the standard solution. A color-developing reagent was prepared by mixing equal volumes of the following 0.1 % alcoholic solutions: aluminum chloride, 4aminoantipyrine, antimony (III) chloride, and diphenylamine. This reagent was used for spraying the plates. For the test solution, 1.0 g of the powdered sample was dissolved in 10 ml of a methanol–water (70:30) mixture and filtered. Depending on the active components of the plant, different mobile-phase compositions were selected to separate various active substances. Procedure: the TLC analysis was performed in three stages: Application: A small amount of the prepared solution was applied to one edge of a TLC plate (coated with a thin layer of silica gel) using a micropipette. After application, the solvent quickly evaporated, leaving small spots on the silica gel. Development: The spotted plate was carefully placed in a chamber containing the mobile phase. The chamber was closed, and the solvent front was allowed to rise evenly. Identification: After the colored spots separated, they were immediately visible. Because many substances are colorless, the plate was treated with the fluorescent reagent and examined under UV light. The retention factor (Rf) was calculated as: Rf = distance traveled by the substance distance traveled by the solvent front Rf=\frac{\text{distance traveled by the substance} }{\text {distance traveled by the solvent front}}Rf =distance traveled by the solvent frontdistance traveled by the substance. The Rf value always lies between 0 and 1 and remains constant only if chromatographic conditions—such as the solvent system, type and thickness of adsorbent, sample volume, and temperature—are unchanged. Results: the experimental results showed that the spot of the test solution corresponded to that of the standard solution, with an Rf value of 0.6. 252 Volume 5, Issue 10: Special Issue (EJAR) ISSN: 2181-2020 MPHAPP THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “MODERN PHARMACEUTICS: ACTUAL PROBLEMS AND PROSPECTS” TASHKENT, OCTOBER 17, 2025 in-academy.uz Conclusions: based on the results obtained, the authenticity of the Glabrus dry extract, when examined by thin-layer chromatography, meets the requirements of the State Pharmacopoeia.