ISOLATION OF THE METABOLITES FROM ASTRAGALUS ALOPECIAS AND DETERMINATION OF THEIR CHEMICAL STRUCTURE

340 Volume 5, Issue 10: Special Issue (EJAR) ISSN: 2181-2020 MPHAPP THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “MODERN PHARMACEUTICS: ACTUAL PROBLEMS AND PROSPECTS” TASHKENT, OCTOBER 17, 2025 in-academy.uz ISOLATION OF THE METABOLITES FROM ASTRAGALUS ALOPECIAS AND DETERMINATION OF THEIR CHEMICAL STRUCTURE Akhmedov A.D.1 Mamadalieva N.Z.1, 2 1Institute of the Chemistry of Plant Substances, Tashkent city, Republic of Uzbekistan 2Alfraganus University, Tashkent city, Republic of Uzbekistan e-mail: [email protected] Tel: (90) 010-61-50 https://doi.org/10.5281/zenodo.17337332 Relevance: Currently, the drugs used in medicine are mainly synthetic drugs, their therapeutic effect is effective and fast, but they have some harmful effects. Therefore, a lot of work is being done in the world to create drugs from relatively harmless plant raw materials. In Uzbekistan, providing our people with harmless, high-quality, affordable drugs remains one of the important tasks of our state policy. Purpose of the study: Astragalus (family Leguminosae) is a large genus widespread in temperate regions of the world, mainly in Eurasia and North America. Astragalus has been used in Central Asian traditional medicine for centuries to treat kidney and bladder stones. Phytochemical studies of Astragalus species have led to the isolation and identification of triterpenoid saponins, flavonoids, phenylpropanoids, alkaloids and some other compounds. In terms of medicinal use and scientific research, our scientific work focuses on the isolation of biologically active substances from the Astragalus alopecias Pall. species of the Astragalus genus. Astragalus alopecias Pall. - locally known as Tulkikabi astragal, is spread out in Central Asia (Tian-Shan, Pamir-Alay, Kopetdog), Afghanistan, Iran, and Pakistan. It is a perennial plant growing to 40-80 cm tall and flowering from May to June. Based on the results of the literature review and examination, it was found that there is almost no available information about the chemical components of this species. We continued our search for natural compounds in the Astragalus genus by studying the aerial parts of A. alopecias collected from Boysuntog (Surkhandarya region, Uzbekistan). Methods and techniques: Thin layer chromatography of the methanol extract of A. alopecias revealed the presence of several flavonoids and triterpene glycosides in the plant. Processing of the methanol extract with ethyl acetate yielded the ethyl acetate fraction. This fraction was subjected to multiple column chromatography to isolate the isoflavones ononine (C22H22O9) (1) and daidzein (C15H10O4) (2). The methanol extract was treated with butanol to obtain a butanol fraction. This fraction was subjected to multiple column chromatography to identify the glycoside cycloalpioside D (C35H58O9) (3). Results: The chemical structure of the substances was determined by mass and NMR spectra. This substance was previously isolated from some Astragalus species. It was first identified from A. alopecias. Conclusions: For the first time, three secondary metabolites were isolated from A. alopecias. Research is currently being conducted to evaluate the biological activities of the isolated compounds. 341 Volume 5, Issue 10: Special Issue (EJAR) ISSN: 2181-2020 MPHAPP THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “MODERN PHARMACEUTICS: ACTUAL PROBLEMS AND PROSPECTS” TASHKENT, OCTOBER 17, 2025 in-academy.uz 1 2 3