ANALYSIS OF FLAVONOID COMPOSITION OF ALLIUM MOTOR BY HPLC

353 Volume 5, Issue 10: Special Issue (EJAR) ISSN: 2181-2020 MPHAPP THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “MODERN PHARMACEUTICS: ACTUAL PROBLEMS AND PROSPECTS” TASHKENT, OCTOBER 17, 2025 in-academy.uz ANALYSIS OF FLAVONOID COMPOSITION OF ALLIUM MOTOR BY HPLC Yuldoshev S.S. National University of Uzbekistan, Tashkent city, Republic of Uzbekistan e-mail: [email protected] https://doi.org/10.5281/zenodo.17337480 Relevance: In recent years, the isolation of bioactive compounds from natural sources and the evaluation of their pharmaceutical potential have become important directions of scientific research. In particular, plants of the genus Allium have long been used in traditional medicine. Their chemical composition has been shown to include flavonoids, phenolic acids, vitamins, and other biologically active compounds. In this context, a comprehensive chemical analysis of Allium motor and the assessment of its pharmaceutical and bioactive properties represent a relevant task. Aim of the study. The main aim of this study was to identify flavonoids present in the extracts of vegetative parts of Allium motor using high-performance liquid chromatography (HPLC), to determine their quantitative proportions, and to substantiate their pharmaceutical significance. Methods. As research objects, ethanol extracts were prepared from the leaves and bulbs of Allium motor. Analytical tests were carried out using an Agilent HPLC system operating with ChemStation software. A diode array detector (DAD) was used at wavelengths of 254 nm, 265 nm, and 281 nm. Standard reference substances such as apigenin, rutin, and resveratrol were applied for comparison. Results. HPLC analysis confirmed the presence of several flavonoid compounds in Allium motor extracts. Their relative contents are presented in Table 1. Table 1. Relative content of flavonoids in Allium motor extract (based on HPLC results) № Compound Retention time (min) Relative content (%) 1 Apigenin 1.71–1.73 18.3 – 24.0 2 Rabinin 1.93–1.94 4.6 – 40.9 3 Rutin 2.35 3.7 – 3.9 4 Resveratrol 7.13 3.7 – 22.3 5 Other flavonoids 1.62–1.81 16.4 – 46.8 The results indicate that Allium motor is rich in flavonoids. Rabinin and apigenin were identified as the main components, while resveratrol and rutin also made up a significant proportion of the composition. The diversity of flavonoids demonstrates the strong antioxidant potential of the plant. Furthermore, the high content of rabinin suggests possible immunomodulatory and antiinflammatory properties. The considerable presence of apigenin supports its sedative and antiallergic activity. The detection of resveratrol indicates potential cardioprotective and anticancer effects. Compared to data on other Allium species reported in the literature, Allium motor appears to be distinguished by its richness in bioactive compounds. Thus, extracts of this plant may represent a promising source for the development of new phytopharmaceuticals. Conclusions. Allium motor is rich in flavonoids, with rabinin, apigenin, rutin, and resveratrol being the main components. The chemical composition of the plant confirms its antioxidant and pharmaceutical potential. The findings provide a scientific basis for the development of new phytopreparations and their use in clinical pharmacy.