MACRO- AND MICROELEMENTS IN THE AERIAL PARTS OF ATRAPHAXIS PYRIFOLIA

394 Volume 5, Issue 10: Special Issue (EJAR) ISSN: 2181-2020 MPHAPP THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “MODERN PHARMACEUTICS: ACTUAL PROBLEMS AND PROSPECTS” TASHKENT, OCTOBER 17, 2025 in-academy.uz MACROAND MICROELEMENTS IN THE AERIAL PARTS OF ATRAPHAXIS PYRIFOLIA A. Yulchiyeva PhD Student (Basic Doctoral Student), Namangan State University Sh.V. Abdullayev Doctor of Chemical Sciences (DSc), Professor, Namangan State University N.G. Abdullajonova Leading Researcher, Institute of Bioorganic Chemistry, Academy of Sciences of Uzbekistan, Doctor of Chemical Sciences (DSc), Professor https://doi.org/10.5281/zenodo.17339569 In our research, the main objective was defined as the study of the chemical components of Atraphaxis pyrifolia, a plant belonging to the Polygonaceae family. In June 2025, we initiated the qualitative and quantitative analysis of macroand microelements in the aerial parts of Atraphaxis pyrifolia, collected in the mountainous areas of Parkent District, Tashkent Region, as part of the study of its chemical components. The analysis of the chemical composition of the studied plant provides an opportunity to draw more precise conclusions about this species. An accurately weighed sample of 0.1000 g was quantitatively transferred into Teflon autoclaves. Subsequently, 3 mL of purified concentrated nitric acid (HNO₃) and 2 mL of purified hydrogen peroxide (H₂O₂) were added. The autoclaves were sealed and placed into a microwave digestion system Berghof (SpeedWave Xpert or a similar microwave oven). The digestion process was carried out according to a specific program set via the instrument interface. The number of autoclaves, as well as the temperature and pressure inside them, were automatically controlled and monitored by the system. Process parameters were displayed on a liquid-crystal display. The digestion was conducted under conditions of moist decomposition, with a minimum temperature of T = 50 °C and a maximum temperature of T = 230 °C, at a maximum pressure of P = 40 bar, for a duration of 35–45 minutes. After digestion, the autoclaves were cooled to room temperature, and the liquid mixture was quantitatively transferred to a volumetric flask of 50 or 100 mL capacity. Each autoclave was rinsed 2–3 times, and the rinsings were combined, after which the flask was filled to the calibration mark with distilled water. The solution was thoroughly mixed, transferred into an autosampler vial, and placed in the designated position of the autosampler. In the program, the position of each vial, the weighed mass, and the dilution factor were entered. The mineralized solution was analyzed using an Avio-200 ICP-OES (Inductively Coupled Plasma Optical Emission Spectrometer, PerkinElmer) to quantitatively determine the macroand microelements, heavy metal salts, and rare metals relative to the corresponding standard samples. At the end of the process, the instrument automatically recalculated the results based on the sample mass and dilution factors, providing the degree of accuracy and the relative standard deviation (RSD) values. Table 1 presents the chemical elemental composition of the aerial parts of Atraphaxis pyrifolia. Table 1 Determination of Macroand Microelement Contents in the Samples by the Avio 200 (ICP-OES) Optical Emission Spectrometric Method Elements Stem Flower Leaf 395 Volume 5, Issue 10: Special Issue (EJAR) ISSN: 2181-2020 MPHAPP THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “MODERN PHARMACEUTICS: ACTUAL PROBLEMS AND PROSPECTS” TASHKENT, OCTOBER 17, 2025 in-academy.uz 1 2 3 4 Al 1,125 1.265 2.437 Ba 0.750 1.248 2.647 B 0.086 0.101 0.102 V 0.064 0.045 0.035 Bi 0 0 0 Fe 1.288 3.744 1.878 Cd 0.033 0.022 0.024 Sr 0.212 0.273 0.608 K* 45.063 126.408 102.265 Ca* 80.440 144.507 536.259 Co 0.033 0.032 0.033 Si 0.434 1.572 0.732 Li 0.067 0.089 0.732 Mg* 3.313 7.582 15.068 Mn 0.182 0.525 2.344 Cu 0.318 0.210 0.140 As 0 0.007 0 Na* 2.388 17.608 4.659 Ni 0.094 0.172 0.132 Pb 0.019 0.001 0 Se 0.216 0.186 0.201 Ag 0 0 0 Sn 0 0 0 Ti 0.083 0.046 0.066 Cr 0.052 0.054 0.052 Zn 0.332 0.424 0.316 P* 4.637 15.152 12.251 Mo 0.014 0.023 0.013 Hg 0 0 0 S 2.384 2.466 2.899 The analysis of the macroand microelements in the aerial parts of Atraphaxis pyrifolia revealed that elements such as potassium (K), calcium (Ca), and magnesium (Mg) were present in higher amounts in the flowers, stems, and leaves of the plant. In addition, aluminum (Al), iron (Fe), sulfur (S), phosphorus (P), and sodium (Na) were also detected in comparatively higher amounts than other elements, although at lower concentrations overall. Importantly, toxic elements such as Pb, Cd, As, and Hg were not detected in the composition of Atraphaxis pyrifolia, and the levels of other heavy metals did not exceed permissible limits. On the contrary, the relatively high content of magnesium, potassium, and calcium in different plant organs highlights the significance of the studied plant as a subject of research.