DETERMINATION OF ANTIOXIDANT AND ANTIRADICAL PROPERTIES OF GOSSITAN AND SAFFLOWER EXTRACTS

491 Volume 5, Issue 10: Special Issue (EJAR) ISSN: 2181-2020 MPHAPP THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “MODERN PHARMACEUTICS: ACTUAL PROBLEMS AND PROSPECTS” TASHKENT, OCTOBER 17, 2025 in-academy.uz DETERMINATION OF ANTIOXIDANT AND ANTIRADICAL PROPERTIES OF GOSSITAN AND SAFFLOWER EXTRACTS Qalandarova G.O. Tashkent Pharmaceutical Institute, Tashkent city, Republic of Uzbekistan e-mail: [email protected] https://doi.org/10.5281/zenodo.17343011 Relevance of the topic: In the study of biochemical reactions associated with the action of free radicals, there is a relationship between changes in tissues in the body's aging, atherosclerosis, type 2 diabetes and cancer. Purpose of work: selective gossitan consists of studying the antioquism as well as antiradicality nature of safflower extracts. To achieve the purpose of the work, we used the following research tasks. Study of solubility and properties of extracted gossitan, gallora extracts. Determination of the antioxidant and antiradicality properties of selected gossitan, gallora extracts. The effect of Gossitan and safflower extracts on LPO and oxygen balance in the body The procedure for conducting research. Determination of the antioxidant property due to the thinning of the adrenaline autooxidation reaction; - Determination of antiradical activity of Ekstrakt saflora and Gossitan by recording the change in optical density of the free radical of DFPG (radical); - Determination of antiradical and antioxidant activity of compounds (quercetin and dihydroquercetin exsample) in relation to hydrogen peroxide according to the method based on the Ruch method; - The effect of quercetin polyphenols, tannins, flavonoids on hypoxia was tested by prepatas, which divided animals into 6 groups. The Clark electrode is positioned so that it is sensitive to the oxygen content in the environment. Results: The antioxidant activity of polyphenols, tannins, flavonoids isolated from plants was determined by thinning the autoxidation reaction of adrenaline in vitro. In this, we examined antioxidant activity in concentrations of 100, 250, 500, 750, 1000mg/ml, dissolving substances extracted from plants in 30% alcohol and water. The substances extracted from plants were compared with the antioxidants of the standard antioxidants quercetin and glyclazide. All polyphenols, tannins, flavonoids isolated from plants have antioxidant properties. Because they all showed higher rates than glyclazide. In them, Gossitan in a concentration of 1000 mg/ml showed the highest antioxidant activity with 37.70%, and Gallora extract with 33.0%. In the perexis oxidation processes of lipids in mitochondrial membranes, the ion channels in the membrane break down, allowing various ions and free radicals to enter and the phenomenon of staining is observed. The addition of Gossitan and Gallora extract reduces the mitochondrial bullshit phenomenon. Conclusion: Based on individual solutions for each of the extracted and selected substances, 30 mcg/ml was selected for in vitro conditions. Substances isolated from plants have been found to have high antioxidant properties.The presence of antiradicality properties of quercitin and rutin flavonoids in the Japanese saffron plant and comparing it to synthetic flavonoids showed quercitin 3.4 times higher, rutin 1.84 times higher activity.