Incidence Of Indoor Bacteria For Food Storage House

Journal of Research and Development Peer Reviewed International, Open Access Journal. ISSN : 2230-9578 | Website: https://jrdrvb.org Volume-17, Issue-9| September 2025 30 Incidence Of Indoor Bacteria For Food Storage House Dr. Vasantrao P. Pawar Dept. of Botany Arts & Science College Bhalod, Tal. Yawal Dist. Jalgaon. (M.S.) [email protected] Manuscript ID: JRD -2025-170906 ISSN: 2230-9578 Volume 17 Issue 9| Pp. 30-31 September 2025 Submitted:17 Aug. 2025 Revised: 27 Aug . 2025 Accepted: 12 Sept. 2025 Published: 30 Sept. 2025 Abstract Microorganism which is including fungi, algae, bacteria virus and organic dust present in air can cause several allergic infections. To study was conducted in 5 different as of indoor food storage house in Bholad to analyze the composition and intensity of bacteria using petri-plate method. Bactria are present in order 1.8x102 to 5.3x106 .Bacteria identified in there are the most dominating species are, .Bacillus Pseudomonas and Ssaphyococcus. The present study investigates the incidence and diversity of airborne bacteria in food storage houses located in Bhalod, District Jalgaon (M.S.). Air samples were collected from five different storage facilities during rainy and winter seasons using the Petri-plate exposure method. A total of four dominant bacterial species were identified: Bacillus subtilis, Staphylococcus aureus, Streptococcus lactis, and Pseudomonas spp. The results revealed higher bacterial concentrations during the rainy season, with Bacillus subtilis (55–85%) and Staphylococcus aureus (56– 59%) being most prevalent. Seasonal variations indicated that microbial load and spore concentration were relatively higher in the winter months. These airborne microorganisms, particularly endotoxinproducing species, pose potential occupational health risks, including respiratory impairments and allergic responses, to workers exposed to grain dust. The findings highlight the importance of regular microbial monitoring in food storage environments to minimize health hazards and ensure safe grain storage practices. KeywordBacteria, air indoor store house, season,bhalod IntroductionThere are several food storage houses in around Bhalod for storing food grains for months. A large quantity of dust is generated in storage grain house therefore causing a potential health risk to workers due to inhalation of grain dust. Bacteira produce endotoxin in which can cause allergies to the farm workers (Williams et.,al.,1964). Which can earlier reports, mostly from abroad revealed that grain dust exposure caused both acute chronic respiratory impairments (Chan-Yeung et al.,1979) .It was also reported the grain dust fever and asthma are very much common amongst the workers rxposed to grain dust (Dvorackova1976) Material and Method Sampling of air was performed from Rainy and Winter season in different pules , cereals and other grains food storage house located at the Bhalod, DistrictJalgaon. Air Sampling. The air sample was taken by the use of Petri-plate method. Two samples were collected from one day per months. Plates were exposed to air 10 minute so that the air borne micro flora can be trapped. The Petri plates were placed at o ft. to 7 ft. height above the ground. Microbiological Examination of the Air: Petri plates containing rose Bengal chloramphenicol agar were exposed to air to trap any spore that falls into them. After exposing the open petri plate for 10 min. the plates were closed and incubated at 25 c for 5 days .For isolation bacteria nutrient agar plates were exposed to air from 3 to 5 minutes and incubated at 37 c for the bacteria are were collected. Quick Response Code: Website: https://jrdrvb.org/ DOI: Creative Commons (CC BY-NC-SA 4.0) This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License, which allows others to remix, tweak, and build upon the work noncommercially, as long as appropriate credit is given and the new creations ae licensed under the idential terms. Address for correspondence: Dr. Vasantrao P. Pawar, Dept. of Botany Arts & Science College Bhalod, Tal. Yawal, Dist. Jalgaon. (M.S.) How to cite this article: Vasantrao P. Pawar.,(2025) Incidence Of Indoor Bacteria For Food Storage House. Journal of Research & Development, 17(9), 30-31. Original Article Journal of Research and Development Peer Reviewed International, Open Access Journal. ISSN : 2230-9578 | Website: https://jrdrvb.org Volume-17, Issue-9| September 2025 31 Bacteria isolated were identified based on the morphological character like colony,colour texture followed by biochemical tests according to the standard protocols Table 1: Bacterial Species of Percentage Bacterial Species Rainy Season Winter season 1 2 3 4 5 1 2 3 4 5 Bacillus subtilis 82 63 30 80 81 72 85 44 69 46 Staphylococcus aurens 56 31 24 54 56 29 70 43 45 37 Streptococcus lactis 14 06 21 15 17 - 26 65 11 05 Pseudomouns species 51 16 34 52 58 28 33 31 43 32 Table 2: Bacterial Identification Sr. No Test Bacillus subtillis Staphylococcus species Streptococcus species Pseudomouns species 1 Gram Staining Rod,G + Coccus G + Coccus G + Rod,G - 2 Culture Characteristics Abundant Opaque white waxy Abundant Opaque Golden growth Thin even growth Abundant thin white, medium tums green 3 Starch Hydrolysis + - - - 4 Lipid Hydrolysis + - - + 5 Litmus milk test Peptonization Acid reduction Acid rapid reduction Rapid Peptonization Result and Discussion During the 6 months research work 4 bacterial species were recorded in 5 different food storage house of Bhalod. The bacteria in were high during rainy season ( Table 1 and 2) .Bacteria like Bacillus subtilis varied form 55 %to 85% and staphylococcus aurens 56% to 59% were high during rainy season .The percentage of Pseudomonas species and Streptococcus species lactis were shown in (table 1). The different spore concentration of air borne microorganism inside he air were higher in winter season compared to rainy season.Our study also reported as higher presence of microorganisms in winter season (Chatopadhyayat et al.,2007) .Bacterial spore concentration in different food grain house were in rainy and winter season. On an average bacteria like Pseudomonas species were in the air which produced endotoxin which were considered as potential agent of occupational disease( Ewa et al.,2005) .In present study the bacteria were present in all sampling potential risk of allergies. References 1. Chan Yeung .M. Wong R and Maclean L.,( 1979). 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