Replication of Genomes in Eukaryotes: Analyses of the Structure and Functions of the Replicative ε Polymerases from Yeasts, Plants and Animals

 Corresponding author: Peramachi Palanivelu Copyright © 2025 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution License 4.0. Replication of Genomes in Eukaryotes: Analyses of the Structure and Functions of the Replicative ε Polymerases from Yeasts, Plants and Animals Peramachi Palanivelu * Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai – 625 021, India. World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 Publication history: Received on 28 April 2025; revised on 31 May 2025; accepted on 03 June 2025 Article DOI: https://doi.org/10.30574/wjarr.2025.26.3.2222 Abstract In all living organisms, genome replication is an indispensable activity in their life cycle. As compared to prokaryotes, genome replication in eukaryotes is much more complex. In eukaryotes, the genome replication is accomplished by a more specialized, multi-protein complex known as replisome. Therefore, for a complete understanding of the replication process in eukaryotes, the structure-function relationship of their replisome complex is important. The eukaryotic replisome complex is mainly composed of 3 replicative polymerases (pols), viz. α, ε and δ. The initiation of genome replication starts with the synthesis of primers by the α pol (a primase), which is followed by the synthesis of leadingand lagging-strands by the next two replicative pols, viz. ε and δ, respectively. Among them, the pol ε is an essential enzyme that links the DNA replication machinery to S-phase checkpoint-control in eukaryotes. The active sites of pol and proofreading (PR) domains of α and δ were analyzed from yeasts, animal and plant sources and reported by this author already [1, 2]. The third replicative enzyme, viz. the pol ε is analyzed and reported here. Interestingly, the yeast, animal and plant ε pols possess almost identical pol, PR and carboxyl terminal domains (CTD) with the same active site amino acids. Their catalytic cores use the same template-binding pair (–YG-), the catalytic amino acid (K), the nucleotide selection amino acid (Q) and similar catalytic metal-binding motifs in the pol domain as reported for the other replicative pols. However, they exhibit poor identities among themselves, e.g., the human pol ε showed only 46.69% and 43.19% identities to the plant (Arabidopsis thaliana) and yeast (Saccharomyces cerevisiae) pol ε sequences, respectively. Furthermore, these eukaryotic ε pols use the same active site amino acids in the PR exonuclease domain, and belong to the DEDD(Y)-superfamily of PR exonucleases. Moreover, similar to the other two replicative enzymes, the ε pols also use similar regulatory Zn2+-binding motifs (ZBMs) in their CTD. In addition, it harbours a unique ZBM in their pol domain. Interestingly, the two invariant motifs, viz –SLYPSand -YGDTDwhich are the characteristic motifs found in the other replicative α and δ pols and B-family of DNA pols, are not found in ε pols. Many specialized, conserved sequence motifs are also identified in the ε pols and discussed. Keywords: Eukaryotic genome replication; DNA polymerase δ; DNA polymerase ε; DNA polymerase ε active sites; Proofreading exonuclease active sites; Saccharomyces cerevisiae; Arabidopsis thaliana; Homo sapiens 1. Introduction In all living organisms, DNA pols play a central role in DNA replication to ensure faithful transmission of genetic information from one generation to another. Therefore, to accomplish this important task in their lifecycle, organisms have evolved and possess a high-fidelity genome replication machinery to preserve and maintain the blueprint of life. The replication machinery possesses several types of DNA pols and repair mechanisms to ensure the duplication of an exact copy of the original genome. To date, five different DNA pols have been characterized in Escherichia coli, eight in S. cerevisiae, and as many as 16 in humans [3]. However, only three of these DNA pols, viz. α, ɛ and δ are involved in the duplication of the nuclear genome in all eukaryotes [4]. Even though more than a dozen different DNA pols have also World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 546 been reported to perform various replicative and repair functions in plant cells, yet the detailed properties of them are still very limited. Whereas the α pol (the primase) is involved in the synthesis of the DNA primers to initiate the DNA duplication process, the other two replicative pols, viz. δ and ɛ, use these DNA primers and replicate the whole genome and make an exact copy of the original genome. All three replicative pols belong to B-family pols which are not only involved in replication, but also in repair any error that may occur during the replication process [5]. The B-family DNA pols are reported to function in both prokaryotes and eukaryotes, e.g., pol II, pol B, pol α, pol ε, pol δ, pol ζ. They are bifunctional enzymes, i.e., in addition to polymerization, they also exhibit 3’→5’ PR exonuclease (PR exo) repair function [6]. Even though the replicative pols have an efficient PR function, mutation(s) that rarely escape the replication and repair processes generate the most important new genetic variants in plants and animals. The structural and functional aspects of the replicative pols, viz. α and δ, from yeasts, higher fungi and animals have been extensively analyzed and reported by this author [1, 2]. In this article, the third replicative pol ɛ from yeasts, higher fungi, plants and animals is analyzed and reported. The DNA replication process is highly conserved in all domains of life [7]. Though genome replication in eukaryotes is known to be incredibly more complex, but it is successfully accomplished by a highly sophisticated and coordinated series of molecular events. The replication process, apart from the essential roles played by the three replicative pols, also depends on the participation of other enzymes and regulatory proteins like, primases, cell-cycle kinases, replicative helicases, single-strand binding proteins (SSBs), additional repair enzymes, ligases, etc. Given the importance of accurate DNA replication, proper functioning of all these enzymes and the accessory proteins is critical to maintain the genome integrity. The first step in genome replication in both prokaryotes and eukaryotes is the initiation. This is accomplished by a multi-structural enzyme and protein complex known as a primosome. The primosome essentially consists of an originof-replication initiator protein, a replicative helicase, a helicase loader, SSBs, a primase, a topoisomerase, etc. [8]. After successful initiation of replication, the replication process is taken over by a next multi-protein complex known as the replisome, which extends the replication process and completes it. In both prokaryotes and eukaryotes, the initiation step is performed by the enzyme, RNA primase, which synthesizes the required RNA primer (short segment of SS-RNA typically of 10-20 nucleotides) to initiate the replication process. However, for eukaryotic replications, in addition to the RNA primer, an additional primer, viz. a DNA primer is also required for further extension, which is provided by the enzyme, DNA pol α. The DNA pol α extends the RNA primers by synthesizing short stretches of DNA, which is used by the pol ε and pol δ for further extension of the DNA synthesis in a processive manner and complete the replication process. During replication, the leading-strand is synthesized by pol ε (pol2), and the lagging-strand is synthesized by pol δ (pol3). During replication the leading-strand is synthesized continuously, whereas the lagging-strand is synthesized discontinuously, in fragments of ~200 nucleotides long, known as the Okazaki fragments (OFs), named after the discoverer. All these OFs are connected together in the next step to form a complete complementary strand by a DNA ligase [9]. 1.1. Eukaryotic ε DNA Polymerases From the above discussions, it is clear that the bulk of the DNA synthesis in eukaryotes is performed by only two of the three replicative polymerases, viz. pols ε and δ. DNA pol ε (also known as Pol2 in yeasts and plants and PolE in humans) completes the leadingand lagging-strand syntheses, respectively. Pol ε was first isolated and characterized from yeast cells as early as 1970 as a nuclear polymerase [10]. It was initially considered to be a form of pol δ that was processive in the absence of proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) [11] (The function of RFC is to load PCNA, the processivity factor of the eukaryotic DNA polymerases δ and ɛ, onto primed DNA templates). Later, it was found that pol ε is an independent replicative enzyme in all eukaryotes. Nowadays, its catalytic subunit is isolated and characterized and its gene is cloned and sequenced from several eukaryotes [11 and references therein]. This enzyme, along with the other two eukaryotic replicative (pols α and δ), are grouped under the B-family polymerases [12]. A great deal of information is available now on the structure and functions of the DNA pols ε from a large number of eukaryotes like yeasts, higher fungi, plants and animals. The plant enzyme from A. thaliana has been characterized in sufficient detail and used for further analysis and comparison. 1.1.1. Subunit structure Interestingly, all the pol ε enzymes from eukaryotes are composed of 4 subunits exhibiting similar subunit structures. Figures 1A, B and C show the subunit structures of the ε pols from yeasts, plants and animals. For example, the yeast enzyme’s 4 subunits are named as, Pol2, Dpb2, Dpb3 and Dpb4 (with the molecular masses of 256, 79, 23 and 22 kDa, respectively) and the plant enzyme also follows more or less the same nomenclature as the yeast enzyme as, Pol2A, Dpb2, Dpb3 and Dpb4 (with the molecular masses of 249, 60, 23 and 24 kDa, respectively), but the human enzyme subunits are named as, PolE1-PolE4 (with the molecular masses of 262, 60, 17 and 12 kDa, respectively). The 4 subunits World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 547 are assembled with a stoichiometry of 1:1:1:1. Thus, the DNA pol ε is a heterotetrameric enzyme, made up of the main catalytic subunit (Pol2/Pol2A/PolE1) which assumes a biloped structure, where the NTD and CTD lopes are connected by a linker region and the non-catalytic subunit (Dpb2/PolE2). The Dpb2/PolE2 subunit mediates the homodimerization of the enzyme to its active form and therefore, is essential for the enzyme activity and cell viability. Genetic and biochemical studies indicate that the putative zinc-finger regions in the catalytic subunit (Pol2/Pol2A/PolE1) are important for its interactions with the Dpb2/PolE2 essential subunit. The other two smaller subunits (Dpb3/PolE3 and Dpb4/PolE4) are found to be nonessential for the catalytic activity of the enzyme, but are found to play important regulatory roles [11]. For example, each of the smaller subunits, viz. PolE3 and PolE4 possess H2A/H2B type histone folds and form a tight PolE3-PolE4 dimer. The dimer interacts with the larger catalytic subunit of the enzyme. (For example, PolE3 interacts with subunits PolE1 and PolE4; and the PolE4 interacts with subunits PolE1 and PolE3 and with the subunit of chromatin remodelling complex, (CHRAC), a multi-protein assembly that regulate gene expression and chromatin structure. Though the non-catalytic subunits are highly conserved from yeasts to humans, they largely differ in their sizes in humans [11 and references therein]. Among the plant ε pols (Pol2), the pol ε from A. thaliana has been the most studied one. The pol ε complex in A. thaliana has been shown to play diverse regulatory roles. For example, it is implicated in DNA replication, DNA repair (nucleotide and base excision repairs), DNA recombination and also in epigenetic gene-silencing activities [11]. Unlike the yeast and human enzymes, the Arabidopsis enzyme possesses two isoenzymes, viz. Pol2A and Pol2B. The Pol2A gene is ~16 kb long (49 exons) and encodes a protein of 2,161 amino acids (pI/Mw: 6.19/2,48,897), whereas the ~13 kb long Pol2B gene with same number of exons (49 exons) encodes a protein of 2,138 amino acids which shows 78.5% identity to Pol2A [13]. Importantly, both the isozymes contain all the motifs that are necessary for a functional Pol2 catalytic subunit. However, the expression of Pol2B has been detected mostly under adverse environmental conditions and loss of function of Pol2B has resulted in no visible phenotype, suggesting that the Pol2A forms the main enzyme [13]. Furthermore, the knockout mutants of the (Pol2A) are found to be lethal, suggesting its importance [14, 12]. The nonenzymatic CTD plays a crucial role and it not only mediates the interaction of the main catalytic subunit with the other three smaller regulatory subunits, but also links the replication machinery to the S-phase checkpoint [15, 16]. However, its overall subunit composition and various domains of its catalytic subunit’s structure are mostly understood from the data available from yeast and human enzymes. Adapted from [11, 12]. The pol ε subunit p17 has been identified as an integral subunit of the chromatin-remodelling factor, CHRAC (Chromatin Accessibility Complex). CHRAC is an ISWI (Imitation Switch), i.e., a chromatin remodelling nuclear-ATPase containing complex that regulates chromatin accessibility and nucleosome spacing. In CHRAC, the p17 forms a dimer with p12 subunit that can interact with histones as well as DNA [17]. Figures 1A, B and C. Subunit structures of the eukaryotic DNA pol ε (S. cerevisiae, A. thaliana and Homo sapiens) (the numbers in brackets indicate molecular masses of the subunits in kDa) 1.1.2. Domain organization of the pol ε catalytic subunit The domain organization of the pol ε’s (pol2) catalytic subunit from the yeast, S. cerevisiae, has been extensively studied and characterized. It contains two pol modules, one in the N-terminal domain (NTD) and the other in the CTD. The NTD pol ε module possesses both the pol and PR exonuclease activities, whereas the CTD mediates the crucial interactions between the three smaller regulatory subunits with the catalytic subunit and also links the replication machinery to the S-phase checkpoint [11]. The pol ε exhibits the highest processivity as compared to the other two replicative pols, viz. pols α, and δ. This high processivity was found to be increased even further by the nonessential subunits, Dpb3 and Dpb4, through their interactions with the replication clamp, the PCNA. Furthermore, the genetic and biochemical studies indicate that the putative ZFMs in the main catalytic subunit of the enzyme are important for their interactions with the other subunits and for the catalytic efficiency of the enzyme [18]. World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 548 The main catalytic subunit of the yeast pol ε holoenzyme is dissected into different functional domains as, NTD (31– 281), PR exonuclease domain (282–527) and the palm (528–950), fingers (769–833), and thumb (951–1186) subdomains of the pol domain (that are organized into a toroid) [19], and a linker domain which connects the pol to the CTD. Figures 2A, 2B and 2C show the various domains and the interactions of the subunits with the CTD of the pols ε from different eukaryotic sources. ZBM, Zinc-binding motif; A & B are CysA and CysB, respectively Figures 2A, B and C. Tentative domain organization the main catalytic subunit of the ε pols from eukaryotes (S. cerevisiae, A. thaliana and Homo sapiens) and their interacting accessory subunits 2. Materials and Methods The protein sequence data of ε DNA pols from yeasts, higher fungi, plants and animals were obtained from PUBMED and SWISS-PROT databases. The advanced version of Clustal Omega was used for protein sequence analysis. Along with the conserved motifs identified by the bioinformatics analysis, and the data already available from biochemical, SDM, cryogenic-Electron Microscopy (cryo-EM) and X-ray crystallographic analyses on the ε pols are used to confirm the possible amino acids at the active sites of the pol, PR exonuclease domains and regulatory motifs. 3. Results and Discussion 3.1. MSA analysis of DNA polymerase ε from various plant sources The DNA pol ε was analyzed from yeasts, higher fungi, plants and animal sources. The structural and functional aspects of this enzyme from yeasts and higher fungal sources were already reported by this author [8]. Fig. 3 shows the MSA of pol ε from various plant sources (only the required regions for the discussions are shown). The model plant, A. thaliana’s sequence is used as the standard and highlighted. All the major domains (NTD, PR Exo, Pol, Linker and CTD) are indicated with arrow marks. Large numbers of highly conserved small and large peptides are found from NTD to CTD. It is interesting to note that the highly conserved active site amino acids are usually embedded within these conserved peptides (highlighted). The NTD is conserved in all, but aligned with some gaps. It harbours 3 conserved -DxDtype metal-binding motifs and 3 polybasic peptides (highlighted) and some are implicated as nuclear localization signal (NLS). The NTD is followed by the PR exonuclease and pol domains. The proposed PR exonuclease active site amino acids are highly conserved in all the enzymes (highlighted) and it belongs to the DEDD-superfamily of exonucleases and uses an invariant Y as proton acceptor during catalysis. This is in complete agreement with the other DNA-dependent DNA pols (DdDps) from prokaryotes and eukaryotes [1, 6]. An invariant motif, –SYLPQGS- (highlighted in yellow) is found within the PR exo region in the plant ε pols as found in yeasts, higher fungi and animals [8]. This sequence is somewhat similar to the characteristic –SLYPSmotif found in the other two eukaryotic replicative DNA pols, α and δ. In both the cases, this motif is implicated in dNTP-binding. In the former, it is found within the proposed PR exo region, whereas in the latter two pols, it is located in the pol region itself. The PR exo domain is followed by the pol domain and A ZBM Dpb2 NTD Exo Palm & Fingers Thumb Linker A CTD B 1 281 527 1187 1308 Dpb3 Dpb4 2222 S. cerevisiae B ZBM Dpb2 NTD Exo Palm & Fingers Thumb Linker A CTD B 1 238 502 1143 1283 Dpb3 Dpb4 2161 A. thaliana C ZBM PolE2 NTD Exo Palm & Fingers Thumb Linker A CTD B 1 266 512 1174 1317 PolE3 PolE4 2286 H. sapiens ZBM, Zinc-binding motif; A & B are CysA and CysB, respectively Figures 2A, B & C. Tentative domain organization the catalytic subunit and its interaction with the other accessory subunits of the DNA pol ε from eukaryotes (S. cerevisiae, A. thaliana and the humans) World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 549 is also highly conserved in all. The proposed template-binding and catalytic pairs, and metal-binding sites are highlighted in yellow and green, respectively. The template-binding pair (-YG-), catalytic amino acid (K) and the nucleotide selection amino acid (Q) are in close agreement with other DdDps and DdRps [6]. Unlike other two eukaryotic replicative pols, the pol ε domain contains a ZBM (highlighted) within. The pol domain is followed by a linker region which connects the pol domain to the CTD. Interestingly, a second putative pol active site amino acids are identified in the CTD and highlighted in yellow. The CTD contains the two typical, highly conserved ZBMs and one of them (CysB) is known to bind the 4Fe-4S cluster (highlighted in orange). It is interesting to note that these 2 ZBMs are found invariably in all three replicative pols at their CTDs. In addition to the Cs which make these ZBMs, there are invariant Cs found throughout the sequence (data not shown) which may be involved in disulphide bond formation. The CTD also contains a –DxDtype metal-binding motif within the CysA. It is interesting to note that the NTD, pol and linker regions contain conserved polybasic peptides within them (highlighted) and are implicated as NLSs. (A NLS is a short amino acid sequence that tags proteins for transport into the nucleus of a cell. NLSs are made up of positively charged amino acids, such as Ks and Rs, that are exposed on the protein's surface, with a consensus sequence of -KK/RxK/Re. g., -PKKKRKVis the first discovered NLS from the SV40 large antigen. Interestingly, the deletion of an NLS usually disrupts the nuclear import process. In the same way, fusing a non-nuclear protein to an NLS often results in the protein being imported into the nucleus). A large number of invariant Ws are found throughout the sequence. CLUSTAL O (1.2.4) MSA of the ε DNA polymerases from various plant sources World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 550 World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 551 tr|A0A8D9D0I3|A0A8D9D0I3_BRACM LDRDLEYAITVEGKMRMDSVSNYDEVKEKIKEKLEKLRDCPIREEGPLIYHLDVAAMYPN 649 tr|A0A3P5YCF9|A0A3P5YCF9_BRACM LDRDLEYAITVEGKMRMDSVSNYDEVKEKIKEKLEKLRDCPIREEGPLIYHLDVAAMYPN 649 sp|F4HW04|DPOE1_ARATH LGRDLEYAITVEGKMRMDSISNYDEVKDEIKEKLEKLRDDPIREEGPLIYHLDVAAMYPN 605 tr|D7KI06|D7KI06_ARALL LGRDLEYAITVEGKMRMDSISNYDEVKDEIKEKLEKLRDDPIREEGPLIYHLDVAAMYPN 599 tr|A0A9Q0FIP5|A0A9Q0FIP5_9ROSI LDRDLQYAIRVEGKMELDSVSNYDEVKNAIKQKLLSLRDDPVREECPLIYHLDVAAMYPN 621 tr|A0A7J7C034|A0A7J7C034_TRIWF LDRDLQYAIRVEGKMELDSVSNYDEVRNAILEKLVRLQDEPTREECPLIYHLDVAAMYPN 606 tr|A0A2C9VYJ6|A0A2C9VYJ6_MANES LDRDLQYAIRVEGKMDLDSVSNYDEIKNAIMEKLVRLRDEPIREECPLIYHLDVAAMYPN 607 tr|A0A2C9VYG0|A0A2C9VYG0_MANES LDRDLQYAIRVEGKMDLDSVSNYDEIKNAIMEKLVRLRDEPIREECPLIYHLDVAAMYPN 606 tr|A0A2N9F976|A0A2N9F976_FAGSY LDRDLQYAIRVEGKMDLESVSNYDEVKNAIME---------------------------- 597 tr|A0A7N2KME6|A0A7N2KME6_QUELO LDRDLQYAIRVEGKMDLELVSNYDEVKNAIMEKLVRLQGEPIRDECPLIYHLDVAAMYPN 605 tr|A0A7I8KHB8|A0A7I8KHB8_SPIIN LDRDIQYAITIEGKMDIDSVSNYEEVKTAILEKLQSLRDHPIRDECPLIYHLDVAAMYPN 606 tr|A0A6I9QIG1|A0A6I9QIG1_ELAGV LDRDLQYAITVEGKMDIDSILNYEDVKNAILEKLIFLRDHPMREECPLIYHLDVAAMYPN 604 tr|A0A8B8JBU2|A0A8B8JBU2_PHODC LDRDLQYAITVEGKMDIDSILNYEDVKNAIMEKLILLRDRPTREECPLIYHLDVAAMYPN 604 tr|A0A8B8JBC2|A0A8B8JBC2_PHODC LDRDLQYAITVEGKMDIDSILNYEDVKNAIMEKLILLRDRPTREECPLIYHLDVAAMYPN 604 tr|A0A8I6WFC8|A0A8I6WFC8_HORVV LDRDLQYAIAVEGKLDIDSVTNYDEVKDAIKQKLVSLRDHPTREECPLIYHLDVAAMYPN 629 tr|A0A452YAR6|A0A452YAR6_AEGTS LDRDLQYAIAVEGKLDIDSVTNYDEVKDAIKQKLVSLRDHPTREECPLIYHLDVAAMYPN 569 tr|A0A3B5XXC3|A0A3B5XXC3_WHEAT LDRDLQYAIAVEGKLDIDSVTNYDEVKDAIKQKLVSLRDHPTREECPLIYHLDVAAMYPN 624 tr|A0A3L6EI43|A0A3L6EI43_MAIZE LDRDLHYVIAVEGKLDIDSVTNYDEVKDAIEQKLVALRDHPIREERPLIYHLDVAAMYPN 602 tr|A0A1D6H8Z0|A0A1D6H8Z0_MAIZE LDRDLHYVIAVEGKLDIDSVTNYDEVKDAIEQKLVALRDHPIREERPLIYHLDVAAMYPN 602 tr|A0A835B357|A0A835B357_9POAL LDRDLQYAISVEGKLDIGSVTNYDEVKDAIKQKLISLRDHPIREERPLIYHLDVAAMYPN 615 tr|A0A835EHQ0|A0A835EHQ0_9POAL LDRDLQYAISVEGKLDIGSVTNYDEVKDAIEQKLISLRDHPIREERPLIYHLDVAAMYPN 620 tr|A0A2S3GPC0|A0A2S3GPC0_9POAL LDRDLQYAISVEGKLDIGSVTNYDEVKDAINQKLVSLRDHPIREERPLIYHLDVAAMYPN 620 tr|A0A368PL62|A0A368PL62_SETIT LDRDLQYAISVEGKLDIASVTNYDEVKDAIKQKLVSLRDHPIREERPLIYHLDVAAMYPN 624 *.**:.*.* :***: : : **:::: * : World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 552 World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 553 tr|A0A8D9D0I3|A0A8D9D0I3_BRACM -NHSVAQDDMDDIEDFCKENK-PGVKGPKP--IARSYEVNKEQFGREQQE---------- 1265 tr|A0A3P5YCF9|A0A3P5YCF9_BRACM -NHSVAQDDMDDIEDFCKENK-PGVKGPKP--IARSYEVNKEQFGREQQE---------- 1265 sp|F4HW04|DPOE1_ARATH -DLPVTKDNVEDIEDFCKENR-PSVKGPKP--IARSYEVNKKQSECEQQE---------- 1213 tr|D7KI06|D7KI06_ARALL -DHPVTKDNVEDIEDFCKENR-PSVKGPKP--IARSYEVNKKQSEREQQE---------- 1230 tr|A0A9Q0FIP5|A0A9Q0FIP5_9ROSI SNH-LADRNVQDLEDFLNQS--SSVNGPRP--IVRSYEVSNGKKSVKKTG---KVDSAEQ 1241 tr|A0A7J7C034|A0A7J7C034_TRIWF INPVTNGEHVEDMEDFGSKTR-SPVNGPRP--VVNSYEFNNKERPVQTIG---QVESLQQ 1227 tr|A0A2C9VYJ6|A0A2C9VYJ6_MANES ADHIMNKENVEDLEDFQNKS--SSKNGPRP--IVRLYEMNNGKCLQNATG---RMDSSQQ 1226 tr|A0A2C9VYG0|A0A2C9VYG0_MANES ADHIMNKENVEDLEDFQNKS--SSKNGPRP--IVRLYEMNNGKCLQNATG---RMDSSQQ 1225 tr|A0A2N9F976|A0A2N9F976_FAGSY TDGVMNEEIVEDLEDFGNKKK-SSVNGPRP--IVRCYEVNNRQHSVKTNG---QVGHLER 1212 tr|A0A7N2KME6|A0A7N2KME6_QUELO ANGVMNEEIVKDLEDFGNKSR-KSVTGPRP--IVRCYEVNNRQNSVKTND---QVGCLQQ 1227 tr|A0A7I8KHB8|A0A7I8KHB8_SPIIN SNHELHEMIIGDMEDIMNKEHHSSTVGPRP--TVRSYAANKENLLANASSPCSKTLDRQQ 1231 tr|A0A6I9QIG1|A0A6I9QIG1_ELAGV -----DEMNVGDMEDLIAKKDV-S-KGPRP--VVHSYEVNKENYSSKQSCPEAGLILSHK 1217 tr|A0A8B8JBU2|A0A8B8JBU2_PHODC -----DGTNVGDMEDLVTKRVV-SEVGLRP--VAHSYEVNKENCSSKQSCPEAGVILNHG 1217 tr|A0A8B8JBC2|A0A8B8JBC2_PHODC -----DGTNVGDMEDLVTKRVV-SEVGLRP--VAHSYEVNKENCSSKQSCPEAGVILNHG 1217 tr|A0A8I6WFC8|A0A8I6WFC8_HORVV -----NLNGTGDIEDLLTSD-----KGLRKVTASHGFNMGKENHPNGLPSAEASLGHCKN 1239 tr|A0A452YAR6|A0A452YAR6_AEGTS -----NLNGTGDIEDLLTSD-----KGLRKTTASHGFNIGKENHPNGSPSAKASLGHCKN 1179 tr|A0A3B5XXC3|A0A3B5XXC3_WHEAT -----NLNGTGDIEDLLTSD-----KGLRKATASHGFNIGKENHPNGSPSAKASLGHCKN 1234 tr|A0A3L6EI43|A0A3L6EI43_MAIZE -----NLNRTGDMEDLLISN-----KDLRKN-PSHGLDIDKENNPNGASVGSG-LNISKK 1210 tr|A0A1D6H8Z0|A0A1D6H8Z0_MAIZE -----NLNRTGDMEDLLISN-----KDLRKN-PSHGLDIDKENNPNGASVGSG-LNISKK 1210 tr|A0A835B357|A0A835B357_9POAL -----NLNGTGDMEDLLVSN-----KDLRKN-SSHGLDIDKENNPNGASVGAG-SNNSKN 1223 tr|A0A835EHQ0|A0A835EHQ0_9POAL -----NLNGTGDMEDLLVSN-----KDLRKN-SSHGLDIDKENNPNGASVGAG-SNNSKN 1228 tr|A0A2S3GPC0|A0A2S3GPC0_9POAL -----NLNGAGDMEDLLMSN-----KDLRKN-SSHGLDIDKENNPNGASVGAG-SNNSKK 1228 tr|A0A368PL62|A0A368PL62_SETIT -----SLNGTGDMEDLLMSN-----KDLRKK-SSHGLDIDKENDPNGASVGAG-SNNSKN 1232 *:**: . . : . .: : tr|A0A8D9D0I3|A0A8D9D0I3_BRACM ---S-------KDPKCDDDISFENIDKNVDYQGWLEVKKRKWKGIVEKKKKRRLGDQRSL 1315 tr|A0A3P5YCF9|A0A3P5YCF9_BRACM ---S-------KDPKCDDDISFENIDKNVDYQGWLEVKKRKWKGIVEKKKKRRLGDQRSL 1315 sp|F4HW04|DPOE1_ARATH ---S-------WDTEF-HDISFQNIDKSVNYQGWLELKKRKWKVTLEKKKKRRLGDLRSS 1262 NLS tr|D7KI06|D7KI06_ARALL ---S-------WDPEF-HDISFQNIDKSVNYQGWLELKKRKWKVTLEKKKKRRLGDLRSP 1279 tr|A0A9Q0FIP5|A0A9Q0FIP5_9ROSI QSSHTAEIHEFSSSLQLNTSPSEDIDRNVDYQGWLVVKKRKWKETLARRKKQRLGSLGTS 1301 tr|A0A7J7C034|A0A7J7C034_TRIWF QTDGK------------NDLFTDNIDKNKDYQGWLELKKRKWKDTLARRKRQRLGSSRTP 1275 tr|A0A2C9VYJ6|A0A2C9VYJ6_MANES QINN----RESIELLQQNASSTESIDRNVDYQGWLELKKRKWKDVLDRRKRQRLGSLRNS 1282 tr|A0A2C9VYG0|A0A2C9VYG0_MANES QINN----RESIELLQQNASSTESIDRNVDYQGWLELKKRKWKDVLDRRKRQRLGSLRNS 1281 tr|A0A2N9F976|A0A2N9F976_FAGSY QTDHSENVLQELPPLQQNSLSSENIDRNVDYQGWLELKKRKWKDTLERRKKQRLSNSRTP 1272 tr|A0A7N2KME6|A0A7N2KME6_QUELO QTDHRL-----------PALSSENIDKNVDYRGWLELKKRKWKDTLERRKRQRLSNSRTP 1276 tr|A0A7I8KHB8|A0A7I8KHB8_SPIIN QEDGAHGKHQPLLSPMENLISTDSIDRNVDYQGWLDAKKRKWKDTRERKKRRRLGAMDGS 1291 tr|A0A6I9QIG1|A0A6I9QIG1_ELAGV QQNA--TLCRPLMSLSQNGTCGESVDRNIDYQAWLEVKKRKWKDTREERKRRRLGITKMS 1275 tr|A0A8B8JBU2|A0A8B8JBU2_PHODC QQNA--TLCRPLLSFNQNDICSEIVDRNVNYQAWLEVRKRKWKDTREERKRRRLGITKMS 1275 tr|A0A8B8JBC2|A0A8B8JBC2_PHODC QQNA--TLCRPLLSFNQNDICSEIVDRNVNYQAWLEVRKRKWKDTREERKRRRLGITKMS 1275 tr|A0A8I6WFC8|A0A8I6WFC8_HORVV QQKSVIRSNEP----LRDDSADERVDRSTDYQGWLEARKRKWKYVREQKKRRRLGAAASS 1295 tr|A0A452YAR6|A0A452YAR6_AEGTS QQKSVIRSNEP----LRDDSADEIVDRSTDYQGWLEARKRKWKYVREQKKRRRLGAAASS 1235 tr|A0A3B5XXC3|A0A3B5XXC3_WHEAT QQKSVIRSNEP----LRDDSADEIVDRSTDYQGWLEARKRKWKYVREQKKRRRLGAAASS 1290 tr|A0A3L6EI43|A0A3L6EI43_MAIZE QQNCMTGLNVPCTSQIQNAASYETVDKGTDYQGWLDAKKRKWKYVREQKKRRSNYAEEQS 1270 tr|A0A1D6H8Z0|A0A1D6H8Z0_MAIZE QQNCMTGLNVPCTSQIQNAASYETVDKGTDYQGWLDAKKRKWKYVREQKKRRRLGAAATF 1270 tr|A0A835B357|A0A835B357_9POAL QRNSKTGLNAPLSSQMPNAAVDETIDRSSDYQGWLDAKKRKWKHVREQKKRRRLGAAATF 1283 tr|A0A835EHQ0|A0A835EHQ0_9POAL QRNSKTGLNAPLSSQMPNVAVDETIDRSSDYQGWLDAKKRKWKHVREQKKRRRLGAAATF 1288 tr|A0A2S3GPC0|A0A2S3GPC0_9POAL QQNSVTGLNVPLSSQIQNVAADETTDRSTDYQGWLDAKKRKWKHVREQKKRRRLGAAATF 1288 tr|A0A368PL62|A0A368PL62_SETIT QQNSITGLNVPLSSQIENAAADESIDRSTDYQGWLDAKKRKWKHVREQKKRRRLGAAATF 1292 : *:. :*:.** :*****.:*:: Linker CTD tr|A0A8D9D0I3|A0A8D9D0I3_BRACM KQIESH----------EIKKKVTQVRRGVGS FFRRPEEALTSSHWQI------------- 1352 tr|A0A3P5YCF9|A0A3P5YCF9_BRACM KQIESH----------EIKKKVTQVRRGVGS FFRRPEEALTSSHWQI------------- 1352 sp|F4HW04|DPOE1_ARATH NQVDTH----------EINQKVGQGRGGVGS YFRRPEEALTSSHWQI------------- 1299 tr|D7KI06|D7KI06_ARALL NQSDAH----------EINQKVGQGRGGVGS YFRRPEEALTSSHWQVCT----------- 1318 tr|A0A9Q0FIP5|A0A9Q0FIP5_9ROSI RGSDGAFEHLGSLGTEKE----AQRKTGVGS YFTRPEVVLTRCHWQ-------------- 1343 tr|A0A7J7C034|A0A7J7C034_TRIWF HRADGASEVMEGMISKRG----DQAKTGVGS YFRRHEVALTRCHWQ-------------- 1317 tr|A0A2C9VYJ6|A0A2C9VYJ6_MANES NRANGASEPLGSLINNKK----AQHRTGVGS YFATHEISLTRCHWQ-------------- 1324 tr|A0A2C9VYG0|A0A2C9VYG0_MANES NRANGASEPLGSLINNKK----AQHRTGVGS YFATHEISLTRCHWQ-------------- 1323 tr|A0A2N9F976|A0A2N9F976_FAGSY HRGNGVSDLLGGVTNGKD----TQGRSGVGS YFRRQEVALTRCHWQ-------------- 1314 tr|A0A7N2KME6|A0A7N2KME6_QUELO RRGKGVLEVLGDVTNHKD----TQGRSGVSS YFKRHEASLTRCHWQ-------------- 1318 tr|A0A7I8KHB8|A0A7I8KHB8_SPIIN SQFVAMAGNHESMTSNKH----NIAKNGAVS FFRRQELLLVHNHWQ-------------- 1333 tr|A0A6I9QIG1|A0A6I9QIG1_ELAGV QQSAGAAKLPASMFNYRRG----QDRNGVSS FFRRQELALVQSHWQ-------------- 1317 tr|A0A8B8JBU2|A0A8B8JBU2_PHODC QQSAGAAKLPASMFNYRHN----QDRSGVSS FFRRQELALVQSHWQ-------------- 1317 tr|A0A8B8JBC2|A0A8B8JBC2_PHODC QQSAGAAKLPASMFNYRHN----QDRSGVSS FFRRQELALVQSHWQ-------------- 1317 tr|A0A8I6WFC8|A0A8I6WFC8_HORVV EGPSNN------LFSARNGSQLHGNGRNRST FFQKQELSLFRSHWQ-------------- 1335 tr|A0A452YAR6|A0A452YAR6_AEGTS EGPSNN------LFSARNVSQLHGNSRNRST FFQKQELSLFRSHWQ-------------- 1275 tr|A0A3B5XXC3|A0A3B5XXC3_WHEAT EGPSNN------LFSARNDSQLHGNGRNRST FFQKQELSLFRSHWQ-------------- 1330 tr|A0A3L6EI43|A0A3L6EI43_MAIZE HVS-LL------L------------------- ---------LPGWVLPPLLTILTLCSRP 1295 tr|A0A1D6H8Z0|A0A1D6H8Z0_MAIZE DDP-NA------LLSARHANQLPVNSRNRST FFQKQELALFRSHWQACV--FCYLLCSVA 1321 tr|A0A835B357|A0A835B357_9POAL DGPTNA------LLSSRNANQLPGNSRNRST FFQKQELALFRSHWQ-------------- 1323 tr|A0A835EHQ0|A0A835EHQ0_9POAL DGPTNA------LLSSRNVNQLPDNSRNRST FFQKQELALFRSHWQ-------------- 1328 tr|A0A2S3GPC0|A0A2S3GPC0_9POAL DGPTNA------LLSSRNVSQLPGNSRNRAT FFQKQELALFRSHWQ-------------- 1328 tr|A0A368PL62|A0A368PL62_SETIT DGPTNA------LLSSRNVNQLPGNSRNRST FFQKQELALFRSHWQ-------------- 1332 * World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 560 Polymerase CTD tr|A0A6Q2WWN8|A0A6Q2WWN8_ESOLU ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1189 tr|A0A673YLH4|A0A673YLH4_SALTR ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1195 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1195 tr|A0A6P6MI98|A0A6P6MI98_CARAU ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1194 tr|A0A498LK69|A0A498LK69_LABRO ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1170 tr|A0A3Q2YTI5|A0A3Q2YTI5_HIPCM ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDVYKQKKISEIFTSEGKRQ 1195 tr|M4A042|M4A042_XIPMA ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1195 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC ERLSSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDVFKQKKISELFTSEGKRQ 1193 tr|A0A3Q2PG22|A0A3Q2PG22_FUNHE ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1195 tr|A0A6A4SMP2|A0A6A4SMP2_SCOMX ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1173 tr|A0A673CZR7|A0A673CZR7_9TELE ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1196 tr|A0A671YAK3|A0A671YAK3_SPAAU ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKKQ 1195 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1195 tr|A0A7N8YN60|A0A7N8YN60_9TELE ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1203 tr|A0A3P8N896|A0A3P8N896_ASTCA ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1194 tr|A0A3B4GLI9|A0A3B4GLI9_9CICH ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1194 tr|A0A6A5F6M3|A0A6A5F6M3_PERFL ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1195 tr|A0A3Q1FLK4|A0A3Q1FLK4_9TELE ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1195 tr|A0A4W6G9K4|A0A4W6G9K4_LATCA ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1195 tr|A0A3B4YTJ5|A0A3B4YTJ5_SERLL ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1195 tr|A0A665X3K2|A0A665X3K2_ECHNA ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFTSEGKRQ 1195 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE ERLGSAVQKIITIPAALQQVRNPVPRVKHPEWLHRKLLE KNDIYKQKKISELFILEGKRQ 1167 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFVLEGRRQ 1186 tr|A0A7J8H4F1|A0A7J8H4F1_ROUAE ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDIYKQKKISELFVLEGRRQ 1200 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF ERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLE KNDVCKQKKISELFVLEGRRQ 1229 sp|Q07864|DPOE1_HUMAN ERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLE KNDVYKQKKISELFTLEGRRQ 1194 tr|A0A2I3S482|A0A2I3S482_PANTR ERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLE KNDVYKQKKISELFTLEGRRQ 1194 tr|A0A2K6CI58|A0A2K6CI58_MACNE ERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLE KNDVYKQKKISELFTLEGRRQ 1171 tr|A0A834DIT3|A0A834DIT3_9CHIR ERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLE KNDIYKQKKISELFVPEGKRQ 1194 tr|F6Z911|F6Z911_HORSE ERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLE KNDIYKQKKISELFILEGKRQ 1194 tr|A0A5G2RB90|A0A5G2RB90_PIG ERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLE KNDVYKQKKISELFVLEGKRQ 1167 tr|A0A670JP16|A0A670JP16_PODMU ERLGSTIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDMYKQKKISELFTSEGKRQ 1192 tr|A0A852LC41|A0A852LC41_UROIN ERLGSTIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDVYKQKKINELFTLQGKKQ 1211 tr|F6ZFB6|F6ZFB6_ORNAN ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLE KNDVYKQKKISELFVSEGKRQ 1069 tr|A0A6P5LNS3|A0A6P5LNS3_PHACI ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLME KHDIYKQKKISELFIHEGKRQ 1195 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR ERLGSAIQKIITIPAALQQVKNPVPRVRHPDWLHKKLME KHDIYKQKKISELFIHEGKRQ 1179 ***.*::*************:******:**:***:**:* *:*: *****.*:* :*::* tr|A0A6Q2WWN8|A0A6Q2WWN8_ESOLU WREILGPPPPTGETQEERLVWLRYHKKKWELQLRQRKERRKRRRLLDGEAQPVGGGVIRD 1305 tr|A0A673YLH4|A0A673YLH4_SALTR WREILGPPPPSGETQEARLVWLRYHKKKWELQLRQRKERRKRRRLQDGEVQAMGGGVIRD 1312 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE WREILGPPPPTGETQEARLVWLRYHKKKWELQLRQRRERRKRRRLLDGEVQAMGGGVIRD 1310 tr|A0A6P6MI98|A0A6P6MI98_CARAU WREILGPPPTIGANREELLVWLRYHKKKWELQLRQRKERRKRRRLVDGESQPTGGGVIRG 1309 tr|A0A498LK69|A0A498LK69_LABRO WREILGPPPAMGATREEILVWLRYHKKKWELQLRQRKERRKRRRLIDGESQPTGGGVIRD 1285 tr|A0A3Q2YTI5|A0A3Q2YTI5_HIPCM WREVLGPPPPMGKTKEEHLVWLRYHKKKWELQMRQRKERKKKRRLLDGEAQPVSGGVIRD 1310 tr|M4A042|M4A042_XIPMA WREILGPPPPTGKTREERLVWLRYHKKKWELQLRQRKEKKKRRKLLDGEPQPVGGGVIRG 1309 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC WREILGAPPAVGNSREEILVWLRYHKKKWELQLRQRKERKKRRRLLDGEAQPVGGGVIRD 1308 tr|A0A3Q2PG22|A0A3Q2PG22_FUNHE WREILGPPPPMGKTREERLVWLRYHKKKWELQLRQRKEKKKRRKLLDGEPQPVGGGVIRG 1310 tr|A0A6A4SMP2|A0A6A4SMP2_SCOMX WREILGPPPPMGKTREERLVWLRYHKKKWELQLRQRKERKKRRRLLDGEAQPVGGGVIRD 1288 tr|A0A673CZR7|A0A673CZR7_9TELE WREILGPPPPKGTTKEERLVWLRYHKKKWELQLRQRKERKKRRRLLDGEAHPSGGGVIRG 1311 tr|A0A671YAK3|A0A671YAK3_SPAAU WREILGPPPPSGKTREERLVWLRYHKKKWELQLRQRKERKKRRRLLDGEAQPVGGGVIRD 1305 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE WREILGPPPPKGTTKEERLVWLRYHKKKWELQLRQRKERQKRRRLLDGEAQPVGGGVIRD 1309 tr|A0A7N8YN60|A0A7N8YN60_9TELE WREILGPPPPMGTTREERLVWLRYHKKKWELQLRQRKERKKRRRLLDGEAQPVSGGVIRD 1318 tr|A0A3P8N896|A0A3P8N896_ASTCA WREILGPPPPTGKTREERQVWLRYHKKKWELQLKQRKERKKRRRLLDGEAQPVGGGVIRG 1309 tr|A0A3B4GLI9|A0A3B4GLI9_9CICH WREILGPPPPTGKTREERQVWLRYHKKKWQLQLKQRKERKKRRRLLDGEAQPVGGGVIRG 1309 tr|A0A6A5F6M3|A0A6A5F6M3_PERFL WREILGPPPPMGKTREERLVWLRYHKKKWELQLRQRKERKKKRRLLDGEAQPVGGGVIRG 1310 tr|A0A3Q1FLK4|A0A3Q1FLK4_9TELE WREILGPPPPTGKTREELLVWLRYHKKKWELQLRQRKERKKRRRLLDGEAQPVGGGVIRD 1307 tr|A0A4W6G9K4|A0A4W6G9K4_LATCA WREILGPPPPMGKTREERLVWLRYHKKKWELQLKQRKERKKRRRLLDGEAQPVGGGVIRD 1310 tr|A0A3B4YTJ5|A0A3B4YTJ5_SERLL WREILGPPPPMGKTREERLVWLRYHKKKWELQLRQRKERKKRRRLLDGEAQPVGGGVIRG 1310 tr|A0A665X3K2|A0A665X3K2_ECHNA WREILGPPPPMGTTREERLVWLRYHKKKWELQLRQRKERKKRRRLLDGEAQPVGGGVIRD 1310 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE WQEVLGRPPALGTTQEEWLVWLRFHKKKWQLQARQRLARRKRRRLEGAEGAPQPGA-IR1281 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA WQEVLGRPPALGTTQEEWLVWLRFHKKKWQLQARQRLARRKRQRLDTAEGAPQPGA-VR1300 tr|A0A7J8H4F1|A0A7J8H4F1_ROUAE WQEVLGRPPALGTTQEEWLVWLRFHKKKWQLQARQRLARRKRRRLDTAKGAPHPGA-VR1314 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF WQEILGQPPALGTTQEEWLVWLRFHKKKWQLQARQRLACKKRRCVEVAEGEPLPGA-IR1343 sp|Q07864|DPOE1_HUMAN WQEILGQPPALGTSQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGA-IR1308 NLS tr|A0A2I3S482|A0A2I3S482_PANTR WQEILGQPPALGTTQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGA-IR1308 tr|A0A2K6CI58|A0A2K6CI58_MACNE WQEILGQPPALGTTQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGA-IR1285 tr|A0A834DIT3|A0A834DIT3_9CHIR WQEILGQPPALGTTQEEWLVWLRFHKKKWQLQARQRLARRKRRRLE-AEGMLQPGA-MR1307 tr|F6Z911|F6Z911_HORSE WQEILGQPPALGTTQEEWLVWLRFHKKKWQLQSRQRLARKKRRRLEVAEGAPRPGA-IR1308 tr|A0A5G2RB90|A0A5G2RB90_PIG WQEILGQPPALGTTQEEWLVWLRFHKKKWQLQARQRLAHRKRRRLEVAEGVPRPGA-IR1281 tr|A0A670JP16|A0A670JP16_PODMU WREILGPPPPSGTTKEERLVWLCYHKKKWEVQARQRQERRKRRRLADGESVLGGGL-IR1304 tr|A0A852LC41|A0A852LC41_UROIN WREILGPPPPLGTTKEQRLVWLRYHQRKWELQAQQRRERRKRRRLEDGGMD--TGT-GR1325 tr|F6ZFB6|F6ZFB6_ORNAN WREILGQPPPLGTTKEERLVWLRFHKKKWELQARQRQERRKRRRLQAGEAAAGGGV-IR1183 tr|A0A6P5LNS3|A0A6P5LNS3_PHACI WREILGQPPSLGTTKEEWLVWLTFHKKKWELQARQRQERRKRRRVETRKGVLGSGV-VR1309 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR WREILGQPPSLGTTKEEWLVWLTFHKKKWELQARQRQERRKRRRVETREGVLGSGV-IR1293 *:*:** ** * .:* *** :*::**::* :** :*:: : * * tr|A0A6Q2WWN8|A0A6Q2WWN8_ESOLU NQKVPKQE-EGASYKKVNRLLPRSNIVYFLYQYSVPEDMYQEHINEINADLSAPDIEGVY 1424 tr|A0A673YLH4|A0A673YLH4_SALTR NQKVPKQE-EGATYRKVNRLLPRSNIVYYLYQYSVPEDMYQEHINEINADLSAPDIEGVY 1431 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE NQKVPKQE-EGATYKKVNRLLPRSNIVYYLYQYSVPEDMYQEHINEINADLSAPDIEGVY 1429 tr|A0A6P6MI98|A0A6P6MI98_CARAU NQKVPKQE-EGANCKKVNRILPRSGVVYYLYQYAVPEDMYQEHINEINADLSAPDIEGVY 1427 tr|A0A498LK69|A0A498LK69_LABRO NQKVPKQE-EAANCKKVNRILPRSGVVYYLYQYAVPEDMYQEHINEINADLSAPDIEGVY 1403 tr|A0A3Q2YTI5|A0A3Q2YTI5_HIPCM NQKVPKQE-EGPTYKKVNRMLPRSNMVYYLYEYCVPEDMYQEHINEINADLSAPDIEGVY 1428 tr|M4A042|M4A042_XIPMA NQKVPKQDEGGVAYRKVNRMLPRSNIVYYLYEYSVPEDMYQNHINEINADLSAPDIEGVY 1428 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC NQKVPKQE-EGATYKKVNRMLPRSNIVYFLYEYSVPEDMYLKHINEINADLSAPDIEGVY 1427 tr|A0A3Q2PG22|A0A3Q2PG22_FUNHE NQRVPKQDEAGAAYRKVNRMLPRSNIVYYLYEYSVPEDMYQKHINEINADLSAPDIEGVY 1429 tr|A0A6A4SMP2|A0A6A4SMP2_SCOMX NQKVPKQE-EGATYRKVNRMLPRSNMVGYLYEYCVPEDMYQEHINEINADLSAPDIEGVY 1406 tr|A0A673CZR7|A0A673CZR7_9TELE NQKVPKQE-EGLTYKKVNRMLPRSNMVYYLYEYSVPEDMYQEHINEINADLSAPDIEGVY 1429 tr|A0A671YAK3|A0A671YAK3_SPAAU NQKVPKQE-EGATYKKVNRMLPRSNMVYYLYEYCVPEDMYQEHINEINADLSAPDIEGVY 1423 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE NQKVPKQE-EGAAYKKVNRMLPRSNMVYYLYEYCVPEDMYQEHINEINADLSAPDIEGVY 1427 tr|A0A7N8YN60|A0A7N8YN60_9TELE NQKVPKQE-EGATYKKVNRMLPRSNMVYYLYEYCVPEDMYQEHINEINADLSAPDIEGVY 1436 tr|A0A3P8N896|A0A3P8N896_ASTCA NQKVPKQE-EGATYKKVNRMLPRSNMVYYLYEYSVPEDMYQEHINEINADLSAPDIEGVY 1427 tr|A0A3B4GLI9|A0A3B4GLI9_9CICH NQKVPKQE-EGATYKKVNRMLPRSNMVYYLYEYSVPEDMYQEHINEINADLSAPDIEGVY 1427 tr|A0A6A5F6M3|A0A6A5F6M3_PERFL NQKVPKQE-EGATYKKVNRMLPRSNIVYYLYEYCVPEDMYQEHINEINADLSAPDIEGVY 1428 tr|A0A3Q1FLK4|A0A3Q1FLK4_9TELE NQKVPKQE-EGPTYKKVNRMLPRSNMVYYLYEYCVPEDMYQEHINEINADLSAPDIEGVY 1425 tr|A0A4W6G9K4|A0A4W6G9K4_LATCA NQKVPKQE-EGATYKKVNRMLPRSNIVYYLYEYCVPEDMYQEHINEINADLSAPDIEGVY 1428 tr|A0A3B4YTJ5|A0A3B4YTJ5_SERLL NQKVPKQE-EGATYKKVNRMLPRSNIVYYLYEYCVPEDMYQEHINEINADLSAPDIEGVY 1428 tr|A0A665X3K2|A0A665X3K2_ECHNA NQKVPKQE-EGATYKKVNRLLPRSNIVYYLYEYCVPEDMYQEHINEINADLSAPDIEGVY 1428 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE NQRVPKAE-EGPSSRKVNRVLPRSHTVYNLYEYSVPEDMYQGHINEISTELSAPDIEGVY 1400 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA NQRVPKAE-EGPSYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELSAPDIEGVY 1419 tr|A0A7J8H4F1|A0A7J8H4F1_ROUAE NQRVPKAE-EGPSYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELSAPDIEGVY 1433 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF NQQVAKAE-EGPSYRKVSRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELSAPDIEGVY 1462 sp|Q07864|DPOE1_HUMAN NQRVAKAE-EGASYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINAELSAPDIEGVY 1427 Exo 2 tr|A0A2I3S482|A0A2I3S482_PANTR NQRVAKAE-EGASYRKVNRVLPRSNVVYNLYEYSVPEDMYQEHINEINAELSAPDIEGVY 1427 tr|A0A2K6CI58|A0A2K6CI58_MACNE NQRVAKAE-EGTSYRKVNRVLPRSNVVYNLYEYSVPEDMYQEHINEINAELSAPDIEGVY 1404 tr|A0A834DIT3|A0A834DIT3_9CHIR NQRVPKAE-EGPAYRKVNRALPRSNMVYNLYEYSVPEDMYQEHINEINTELSAPDIEGVY 1426 tr|F6Z911|F6Z911_HORSE NQRVAKAE-EGPSYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELSAPDIEGVY 1427 tr|A0A5G2RB90|A0A5G2RB90_PIG NQRVAKVE-ESPSYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELAAPDIEGVY 1400 tr|A0A670JP16|A0A670JP16_PODMU NQRVPKPE-ESTAYRKVNRILPRSNLVYNLYEYSVPEEMYQEHINEINASLSAPDIEGVY 1423 tr|A0A852LC41|A0A852LC41_UROIN NQRVPKPE-EGAAYRKVNRILPRSNLVYNLYEYSVPEDMYQEHINEINADLSAPDIEGVY 1444 tr|F6ZFB6|F6ZFB6_ORNAN NQRVAKAE-EGATFRKVNRILPRSNVVYHLYEYSVPEDMYQEHINEINADLSAPDIEGVY 1302 tr|A0A6P5LNS3|A0A6P5LNS3_PHACI NQRVPKEE-DGPSFHKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINADLSAPDIEGVY 1428 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR NQRVPKEE-DGPSFRKVNRVLPRSNVVYNLYEYSVPEDMYQEHINEINADLSAPDIEGVY 1412 **:* * : . :**.* **** * **:*.***:** *****.:.*:******** World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 561 tr|A0A6Q2WWN8|A0A6Q2WWN8_ESOLU ETQVPLLFRALVQLGCVCMVNKQVVRALGGREAEAYDLEHLEMRSLAQFSYLEPGSVRHI 1484 tr|A0A673YLH4|A0A673YLH4_SALTR ETQVPLLFRALVQLGCMCMVNKQLVRDLGGREAETYDLEHLEMRSLAQFSYLEPGSVRHI 1491 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE ETQVPLLFRALVQLGCMCMVNKQLVRDLGGREAETYDLEHLEMRSLAQFSYLEPGSVRHI 1489 tr|A0A6P6MI98|A0A6P6MI98_CARAU ETQVPLLFRALVQLGCMCMVNKQVVRDLAGREADTFDLEHLEMRSLAQFSYLEPGSVRHI 1487 tr|A0A498LK69|A0A498LK69_LABRO ETQVPLLFRALVQLGCMCMVNKQVVRDLAGREADTFDLDHLEMRSLAQFSYLEPGSVRHI 1463 tr|A0A3Q2YTI5|A0A3Q2YTI5_HIPCM ETQVPLLFRALVQLGCVCMVNKRVGRDMAGREADTFDLENLDMRSLAQFSYLEPGSVRHM 1488 tr|M4A042|M4A042_XIPMA ETQVPLLFRALVQLGCVCMVNKHIVRDLAGRETDSFDLEHLDMRSLAQFSYLEPGSVRHM 1488 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC ETQVPLLFRALVQLGCVCMVNKHVVRDMAGREADTFDLENLEMRSLAQFSYLEPGSVRHM 1487 tr|A0A3Q2PG22|A0A3Q2PG22_FUNHE ETQVPLLFRALVQLGCVCMINKHAARDLAGRESDSYDLAHLEMRSLAQFSYLEPGSVRHM 1489 tr|A0A6A4SMP2|A0A6A4SMP2_SCOMX ETQVPLLFRALVKLGCVCMVNKHVVRDLAGREADSFDLEHLEMRSLAQFSYLEPGSVRHM 1466 tr|A0A673CZR7|A0A673CZR7_9TELE ETQVPLLFRALVQLGCVCMVNKHVVRDLAGREADTFDLDHLEMRSLAQFSYLEPGSVRHM 1489 tr|A0A671YAK3|A0A671YAK3_SPAAU ETQVPLLFRALVRLGCVCMVNKHVVRDLAGREADTFDLEHLEMRSLAQFSYLEPGSVRHM 1483 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE ETQVPLLFRALVQLGCVCMVNKHVVRDLAGREADTFDLEHLEMRSLAQFSYLEPGSVRHI 1487 tr|A0A7N8YN60|A0A7N8YN60_9TELE ETQVPLLFRALVQLGCVCMVNKHAVRDLAGREADTFDLDQLEMRSLAQFSYLEPGSVRHM 1496 tr|A0A3P8N896|A0A3P8N896_ASTCA ETQVPLLFRALVQLGCVCMVNKHVVRDMAGREADTFDLEHLEMRSLAQFSYLEPGSVRHM 1487 tr|A0A3B4GLI9|A0A3B4GLI9_9CICH ETQVPLLFRALVQLGCVCMVNKHVVRDMAGREADTFELEHLEMRSLAQFSYLEPGSVRHM 1487 tr|A0A6A5F6M3|A0A6A5F6M3_PERFL ETQVPLLFRALVQLGCVCMVNKHVVRDLAGREADTFDLEHLEMRSLAQFSYLEPGSVRHM 1488 tr|A0A3Q1FLK4|A0A3Q1FLK4_9TELE ETQVPLLFRALVQLGCVCMVNRLVVRDLAGREADTFDLEHLEMRSLAQFSYLEPGSIRHM 1485 tr|A0A4W6G9K4|A0A4W6G9K4_LATCA ETQVPLLFRALVQLGCVCMVNKHVVRDLAGREADTFDLEHLEMRSLAQFSYLEPGSVRHM 1488 tr|A0A3B4YTJ5|A0A3B4YTJ5_SERLL ETQVPLLFRALVQLGCVCMVNKYVVRDLAGREADTFDLEHLEMRSLAQFSYLEPGSVRHM 1488 tr|A0A665X3K2|A0A665X3K2_ECHNA ETQVPLLFRALVQLGCVCMVNKHVVRDLAGREADTFDLEHLEMRSLAQFSYLEPGSVRHM 1488 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE ETQVPLLFRALVQLGCVCVVSKQLRRHLSGWETDAFTLEHLEMRSLAQFSYLEPGSIRHI 1460 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA ETQVPLLFRALVQLGCVCVVNKQLARHLSGWETETFALEHLEMRSLAQFSYLEPGSIRHI 1479 tr|A0A7J8H4F1|A0A7J8H4F1_ROUAE ETQVPLLFRALVQLGCVCVVNKQLARHLSGWETETFALEHLEMRSLAQFSYLEPGSIRHI 1493 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF ETQVPLLFRALVQLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHL 1522 sp|Q07864|DPOE1_HUMAN ETQVPLLFRALVHLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHI 1487 tr|A0A2I3S482|A0A2I3S482_PANTR ETQVPLLFRALVHLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHI 1487 tr|A0A2K6CI58|A0A2K6CI58_MACNE ETQVPLLFRALVHLGCVCVVSKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHI 1464 tr|A0A834DIT3|A0A834DIT3_9CHIR ETQVPLLFRALVHLGCVCVVSKQLVRHLSGWEAETFSLEHLEMRSLAQFSYLEPGSIRHM 1486 tr|F6Z911|F6Z911_HORSE ETQVPLLFRALVQLGCVCVVNKQLVRQLSGREAETFTLEHLEMRSLAQFSYLEPGSIRHI 1487 tr|A0A5G2RB90|A0A5G2RB90_PIG ETQVPLLFRALVQLGCVCVVNKHLVRHLSGWEAETFALEHLEMRSLAQFNYLEPGSIRHI 1460 tr|A0A670JP16|A0A670JP16_PODMU ETQIPLLLRALIRLGCVCMVNRNLVRHLTGREAETFNLEHLEMRSLAQFSYLEPGSIRHI 1483 tr|A0A852LC41|A0A852LC41_UROIN ETQVPLLLRALIQLGCVCMVSRQLVRHLAGREAEAFDIEHLEMRSLAQFSYLEPGSIRHI 1504 tr|F6ZFB6|F6ZFB6_ORNAN ETQVPLLQRALVQLGCVCMVSRQLAKHLMGREAETFELEHLEMRSLAQFSYLEPGSIRHI 1362 tr|A0A6P5LNS3|A0A6P5LNS3_PHACI ETQVPLLLRALIQLGCVCVVSRQLVQHLTGREAETFALEYLEMRSLAQFSYLEPGSIRHI 1488 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR ETQVPLLLRALIQLGCVCMVNRQLVQHLTGREAETFPLEYLEMRSLAQFSYLEPGSIRHT 1472 ***:*** ***::***:*::.: : : * *:::: : *:*******.******:** tr|A0A6Q2WWN8|A0A6Q2WWN8_ESOLU YLYHHTQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLSTLYGAERTALLEKTSEELLP 1544 tr|A0A673YLH4|A0A673YLH4_SALTR YLYHHSQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLNTLYGAERTALLEKTSEELLP 1551 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE YLYHHSQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLSTLYGAERIALLEKTSEELLP 1549 tr|A0A6P6MI98|A0A6P6MI98_CARAU YLYHHSQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLSSLYGAERTALLEKTTEELLP 1547 tr|A0A498LK69|A0A498LK69_LABRO YLYHHSQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLSSLYGAERTALLEKTTEDLLP 1523 tr|A0A3Q2YTI5|A0A3Q2YTI5_HIPCM YLYHHSQGHKALFGLFIPSQRKANIFILDTVRSNQMPNLSNVYSAERTALLEKTTEDLLP 1548 tr|M4A042|M4A042_XIPMA YLYHHSQGQKALFGLFIPSQRKASVFILDTVRSNQMPNLSNVYTAERTALLEKTSEELLP 1548 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC YLYHHSQGHKALFGLFIPSQRKASIFILDTVRTNQMPNLTNLYTAERIALLEKTSEELLP 1547 tr|A0A3Q2PG22|A0A3Q2PG22_FUNHE YLYHHSQGQKALFGLFIPSQRKASIFVLDTVRSNQMPNLSNLYTAERIALLEKTSEELLP 1549 tr|A0A6A4SMP2|A0A6A4SMP2_SCOMX YLYHHSQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLNNLYTAERTALLEKTTEDLLP 1526 tr|A0A673CZR7|A0A673CZR7_9TELE YLYHHSQGHKALFGLFIPSQRKASIFILDTVRSNQMPNLSNVYTAERTALLEKTTEELLP 1549 tr|A0A671YAK3|A0A671YAK3_SPAAU YLYHHSQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLSNLYTAERTALLEKTTEELLP 1543 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE YLYHHSQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLSNLYTAERTALLEKTTEDLLP 1547 tr|A0A7N8YN60|A0A7N8YN60_9TELE YLYHHSQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLSNLYTTERTALLEKTTEELLP 1556 tr|A0A3P8N896|A0A3P8N896_ASTCA YLYHHSQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLSNLYTAERTALLEKTTEELLP 1547 tr|A0A3B4GLI9|A0A3B4GLI9_9CICH YLYHHSQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLSNLYTAERTALLEKTTEELLP 1547 tr|A0A6A5F6M3|A0A6A5F6M3_PERFL YLYHHSQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLTNLYTAERTALLEKTTEELLP 1548 tr|A0A3Q1FLK4|A0A3Q1FLK4_9TELE YLYHHCQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLSNLYTAERTALLEKTTEELLP 1545 tr|A0A4W6G9K4|A0A4W6G9K4_LATCA YLYHHSQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLSNLYTTERTALLEKTTEELLP 1548 tr|A0A3B4YTJ5|A0A3B4YTJ5_SERLL YLYHHRQGHKALFGLFIPSQRKASIFVLDTVRSNQMPNLSNLYTAERTALLEKTTEELLP 1548 tr|A0A665X3K2|A0A665X3K2_ECHNA YLYHHSQGHKALFGLYIPSQRKASIFVLDTVRSNQMPNLSNLYTAERTALLERTTEELLP 1548 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE YLYHRTQGHKALFGIFVPSQRRASVFVLDTVRSNQMPNLSALYTAEHGLLLDKVGPELLP 1520 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA YLYHHTQGHKALFGIFVPSQRRATVFVLDTVRSNQMPSLSALYSAEHSLLLERVGPELLP 1539 tr|A0A7J8H4F1|A0A7J8H4F1_ROUAE YLYHHTQGHKALFGIFVPSQRRATVFILDTVRSNQMPSLGALYSAEHSLLLERVGPELLP 1553 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF YLYHHAQGHKALFGLFVPSQRRASVFVLDTVRSNQMPSLSALYSAEHSLLMEKVGPELLP 1582 sp|Q07864|DPOE1_HUMAN YLYHHAQAHKALFGIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHGLLLEKVGPELLP 1547 tr|A0A2I3S482|A0A2I3S482_PANTR YLYHHAQAHKALFGIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHSLLLEKVGPELLP 1547 tr|A0A2K6CI58|A0A2K6CI58_MACNE YLYHHAQGHKALFGIFIPSQRRASVFVLDTVRSNQMPNLGALYSAEHGLLLEKVGPELLP 1524 tr|A0A834DIT3|A0A834DIT3_9CHIR YLYHHTQGHKALFGIFVPSQRRASVFVLDTVRSNQMPSLSALYSAEHSLLLEKVGPELLP 1546 tr|F6Z911|F6Z911_HORSE YLYHHAQGHKALFGIFVPSQRRVSVFVLDTVRSNQMPSLSALYSAEHSLLLEKVGPELLP 1547 tr|A0A5G2RB90|A0A5G2RB90_PIG YLYHHMQGHKALFGIFVPSQRKASVFVLDTVRSNQMPSLSALYSAEHSLLLEKVGPELLP 1520 tr|A0A670JP16|A0A670JP16_PODMU YLYHKSQGNKALFGLFIPSQRKASVFVLDTVRSNQMPNLNNMYSAEHSAMVAKVGEELLP 1543 tr|A0A852LC41|A0A852LC41_UROIN YLYHSSQGSKALLGLFIPSQRKAAVFVLDKVRSNQMPNLTSLFSAERSALLAKAGEELLP 1564 tr|F6ZFB6|F6ZFB6_ORNAN YLYHHSQGSKALFGLFIPSQRKASVFVLDTVRSNQMPNLNSMYSSEHSLLLGKVGPDLLP 1422 tr|A0A6P5LNS3|A0A6P5LNS3_PHACI FLYHHSQGSKALFGLFIPSQRKASVFVLDTVRSNQMPNLNSMYSSEHRLLLEKVGPELLP 1548 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR FLYHHSQGSKALFGLFIPSQRKASVFVLDTVRSNQMPNLNSMYSSEHRLLLEKVGPELLP 1532 :*** *. ***:*:::****:. :*:**.**:****.* :: :*: :: :. :*** tr|A0A6Q2WWN8|A0A6Q2WWN8_ESOLU VVPVHVVDEISYNVLDWQRHGARRMIRHYLNLDSCLSQAFDMARYYHLPVGNLPLDVSIF 1664 tr|A0A673YLH4|A0A673YLH4_SALTR VVPVHMVDEISYNVLDWQRHGARRMVRHYLNLDSCLSQAFDMARYYHLPVGNLPHDVSIF 1671 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE VVPVHVVDEISYNVLDWQRHGARRMVRHYLNLDSCLSQAFDMARYYHLPVGNLPQDVSIF 1669 tr|A0A6P6MI98|A0A6P6MI98_CARAU VVPVHVIDEISYNVLDWQRHGARRMIKHYLNLDSCLSQAFDMARYYHLPVGNLPQDISIF 1667 tr|A0A498LK69|A0A498LK69_LABRO VVPVHVIDEISYNVLDWQRHGARRMIKHYLNLDSCLSQAFDMARYYHLPVGNLPQDISIF 1643 tr|A0A3Q2YTI5|A0A3Q2YTI5_HIPCM VMPVHVIDEISYSVLDWQRHGARRMIRHYLNLDSYLSQAFDMARYYHLPVGNLPQDVSIF 1668 tr|M4A042|M4A042_XIPMA VVPVHVVDEINYNVLDWQRHGARRMIRHYLNLDSCLSQAFDMARYYHLPVGNLPQDVSIF 1668 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC VVPVHVVDEISYNVLDWQRHGARRMIRHYLNLDSCLSQAFDMARYYHLPVGNLPQDVSIF 1667 tr|A0A3Q2PG22|A0A3Q2PG22_FUNHE VVPVHVVDEISYNVLDWQRLGARRMVRHYLNLDSCLSQAFDMARYYHLPVGNLPQDVSIF 1669 tr|A0A6A4SMP2|A0A6A4SMP2_SCOMX VVPVHVVDEISYNVLDWQRHGARRMIRHYLNLDSCLSQAFDMARYYHLPVGNLPQDVSIF 1646 tr|A0A673CZR7|A0A673CZR7_9TELE VVPVHVVDEISYNVLDWQRHGARRMIRHYLNLDSCLSQAFDMARYYHLPVGNLPQDVSIF 1669 tr|A0A671YAK3|A0A671YAK3_SPAAU VVPVHVIDEINYNVLDWQRHGARRMIRHYLNLDSCLSQAFDMARYYHLPVGNLPQDVSIF 1663 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE VVPVHVVDEISYNVLDWQRHGARRLIRHYLNLDSCLSQAFDMARYYHLPVGNLPQDVSIF 1667 tr|A0A7N8YN60|A0A7N8YN60_9TELE VVPVHVVDEISYNVLDWQRHGARRMIRHYLNLDSCLSQAFDMARYYHLPVGNLPHDVSIF 1676 tr|A0A3P8N896|A0A3P8N896_ASTCA VVPVHVVDEISYNVLDWQRLGARRMIRHYLNLDSCLSQAFDMARYYHLPVGNLPQDVSIF 1667 tr|A0A3B4GLI9|A0A3B4GLI9_9CICH VVPVHVVDEISYNVLDWQRLGARRMIRHYLNLDSCLSQAFDMARYYHLPVGNLPQDVSIF 1667 tr|A0A6A5F6M3|A0A6A5F6M3_PERFL VVPVHVVDEISYNVLDWQRHGARRMIRHYLNLDSCLSQAFDMARYFHLPVGNLPQDVSIF 1668 tr|A0A3Q1FLK4|A0A3Q1FLK4_9TELE VVPVHVVDEISYNVLDWQRHGARRMIRHYLNLDSCLSQAFDMARYYHLPVGNLPQDVSIF 1665 tr|A0A4W6G9K4|A0A4W6G9K4_LATCA VVPVHVVDEISYNVLDWQRHGARRMIRHYLNLDSCLSQAFDMARYYHLPVGNLPQDVSIF 1668 tr|A0A3B4YTJ5|A0A3B4YTJ5_SERLL VVPVHVADEISYNVLDWQRHGARRMIRHYLNLDSCLSQAFDMARYYHLPVGNLPQDVSIF 1668 tr|A0A665X3K2|A0A665X3K2_ECHNA VVPVHVVDEISYNVLDWQRHGARRMVRHYLNLDSCLSQAFDMARYYHLPVGNLPQDVSIF 1668 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE LVPIRVADHISYSVLDWQRHGARHMLRHYLNLDTCLSQAFEMSRYFHVPVGNLPEDISTF 1640 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA LVPIRVANKISYGVLDWQRHGARHMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTF 1659 tr|A0A7J8H4F1|A0A7J8H4F1_ROUAE LVPVRVANKISYGVLDWQRHGARHMIRHYLNLDTCLSQAFEMSRYFHVPIGNLPEDISTF 1673 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF LVPVHVADKISYGVLDWQRLGARRMIRHYLNLDTSLSQAFEMSRYFHIPIGNLPEDISTF 1702 sp|Q07864|DPOE1_HUMAN LVPICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTF 1667 Exo 2 tr|A0A2I3S482|A0A2I3S482_PANTR LVPICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTF 1667 tr|A0A2K6CI58|A0A2K6CI58_MACNE QVPICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTF 1644 tr|A0A834DIT3|A0A834DIT3_9CHIR LVPVRVADKISYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTF 1666 tr|F6Z911|F6Z911_HORSE LVPVRMVDKVSYGVLDWQRHGAQRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTF 1667 tr|A0A5G2RB90|A0A5G2RB90_PIG LVPIHVADKISYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPVGNLPEDISTF 1640 tr|A0A670JP16|A0A670JP16_PODMU LVPVQVADDVNYSILDWQRHAARHMIRRYLSLDTCLSQAFEMSRYYHIPIGNLPDDISTF 1663 tr|A0A852LC41|A0A852LC41_UROIN LVPIRLLDDVSYSVLDWQRHAARRMIRHYLSLDSCLSQAFEMSRYYHIPVGNLPDDISTF 1684 tr|F6ZFB6|F6ZFB6_ORNAN LVPICVADEINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPDDISTF 1542 tr|A0A6P5LNS3|A0A6P5LNS3_PHACI LVPVCVTDDISYGALDWQRHAARRMIRHYLNLDTCLSQAFEMSRYFHIPVGNLPEDISTF 1668 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR LVPVCVTDDISYGALDWQRHAARRMIRHYLNLDTCLSQAFEMSRYFHIPVGNLPEDISTF 1652 :*: : :.:.*. ***** .*::::::**.**: *****:*:**:*:*:**** *:* * World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 562 ZBM-CTD-Pol tr|A0A6Q2WWN8|A0A6Q2WWN8_ESOLU GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMEADDRGSMEINAQGCYSTVCV 1724 tr|A0A673YLH4|A0A673YLH4_SALTR GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMEADDRGSMEINAQGCYSTVCV 1731 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMEADDRGSMEINAQGCYSTVCV 1729 tr|A0A6P6MI98|A0A6P6MI98_CARAU GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMESDERGSMEINSHGCYSTVCV 1727 tr|A0A498LK69|A0A498LK69_LABRO GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMESDERGSMEINSHGCYSTVCV 1703 tr|A0A3Q2YTI5|A0A3Q2YTI5_HIPCM GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMESDDRGSVEINAQGCYSTVCV 1728 tr|M4A042|M4A042_XIPMA GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMENDEKGSVEINSQGCYSTACV 1728 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMESDDRGSVEINAQGCYSTVCV 1727 tr|A0A3Q2PG22|A0A3Q2PG22_FUNHE GSDLFLARHLRKHNHLLWLSPTVRPDLGGKEADDSRLVMENDERGSVEINSQGCYSTACV 1729 tr|A0A6A4SMP2|A0A6A4SMP2_SCOMX GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMESEDRGSVEINAQGCYSTVCV 1706 tr|A0A673CZR7|A0A673CZR7_9TELE GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMESDDRCSVEINAQGCYSTVCV 1729 tr|A0A671YAK3|A0A671YAK3_SPAAU GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMENDERGSVEMNASGCYSTVCV 1723 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE GSDLFLARHLRKHNHLLWFSPTARPDLGGKEADDSRLVMESDDRGSVEINAQGCYSTACV 1727 tr|A0A7N8YN60|A0A7N8YN60_9TELE GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMESDDRGSVEINAQGCYSTVCV 1736 tr|A0A3P8N896|A0A3P8N896_ASTCA GSDLFLARHLRKHNHLLWLSPTSRPDLGGKEADDSRLVMENDDRGSVEINAQGCYSTACV 1727 tr|A0A3B4GLI9|A0A3B4GLI9_9CICH GSDLFLARHLRKHNHLLWLSPTSRPDLGGKEADDSRLVMENDDRGSVEINAQGCYSTACV 1727 tr|A0A6A5F6M3|A0A6A5F6M3_PERFL GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMESDDRGSVEINAQGCYSTVCV 1728 tr|A0A3Q1FLK4|A0A3Q1FLK4_9TELE GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMESDERGSMEINAQGCYSTVCV 1725 tr|A0A4W6G9K4|A0A4W6G9K4_LATCA GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMESDDRGSVEINAQGCYSTVCV 1728 tr|A0A3B4YTJ5|A0A3B4YTJ5_SERLL GSDLFLARHLRKHNHLLWLSPTARPDLGGKEADDSRLVMESDDRSSVEINAQGCYSTVCV 1728 tr|A0A665X3K2|A0A665X3K2_ECHNA GSDLFLARHLRKHNHLLWLSPTSRPDLGGKEADDSRLVMESDDRSTVEINSQGCYSTVCV 1728 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE GSDLFFARHLQCHNHLLWLSPTPRPDLGGKEADDNRLVMEFDDQATVEINSPGCHSTVCV 1700 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA GSDLFFARHLQRHNHLLWLSPTSRPDLGGKEADDNRLVMEFEDQATVEINSSGCYSTVCV 1719 tr|A0A7J8H4F1|A0A7J8H4F1_ROUAE GSDLFFARHLQRHNHLLWLSPTSRPDLGGKEADDNRLVMEFEDQATVEINSSGCYSTVCV 1733 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF GSDLFFARHLQRHSHLLWLSPTSRPDLGGKEADDNRLVMEFDDQASVEINCPGCYSTVCV 1762 sp|Q07864|DPOE1_HUMAN GSDLFFARHLQRHNHLLWLSPTARPDLGGKEADDNCLVMEFDDQATVEINSSGCYSTVCV 1727 tr|A0A2I3S482|A0A2I3S482_PANTR GSDLFFARHLQRHNHLLWLSPTARPDLGGKEADDNYLVMEFDDQATVEINSSGCYSTVCV 1727 tr|A0A2K6CI58|A0A2K6CI58_MACNE GSDLFFARHLHRHNHLLWLSPTARPDLGGKEADDNCLVMEFDDQATVEINSSGCYSTVCV 1704 tr|A0A834DIT3|A0A834DIT3_9CHIR GSDLFFARHLQRHNHLLWLSPTCRPDLGGKEADDNRLVMEFEDQATMEINSSGCYSTVCV 1726 tr|F6Z911|F6Z911_HORSE GSDLFFARHLQRHNHLLWLSPTSRPDLGGKEADDNRLIMEFEDQATVEINSSGCYSTVCV 1727 tr|A0A5G2RB90|A0A5G2RB90_PIG GSDLFFARHLQRHNHLLWLSPTSRPDLGGKEADDNRLVMEFDDQAPVEINSSGCYSTVCV 1700 tr|A0A670JP16|A0A670JP16_PODMU GTDLFFSRHLHRHNHLLWLSPTSRPDLGGKEADDSRLVMEFDERVSVEINNPGCYSTVCV 1723 tr|A0A852LC41|A0A852LC41_UROIN GSDLFFSRHLRRHNHLLWLSPTARPDLGGKEADDNRLVMEFDERASVEINNPGCYSTVCL 1744 tr|F6ZFB6|F6ZFB6_ORNAN GSDLFFSRHLRRHNHLLWFSPTSRPDLGGKEADDNRLVMEFDDKASVEINNSGCYSTVCL 1602 tr|A0A6P5LNS3|A0A6P5LNS3_PHACI GSDLFFSRHLRHHNHLLWFSPTSRPDLGGKEADDNRLVMEFDDQASVEINNPGCYSTVCL 1728 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR GSDLFFSRHLRHHNHLLWFSPTSRPDLGGKEADDNRLVMEFDDQASVEINNPGCYSTVCL 1712 *:***::***: *.****:*** ***********. *:** ::: :*:* **:**.*: ZBM-CTD-Pol tr|A0A6Q2WWN8|A0A6Q2WWN8_ESOLU CSNTFSRILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHSMMK 1844 tr|A0A673YLH4|A0A673YLH4_SALTR CSNTF-RILKSMVVGWVREITRYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHNMMK 1850 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE CSNTF-RILKSMVVGWVREITRYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHNMMK 1848 tr|A0A6P6MI98|A0A6P6MI98_CARAU CSNTF-RILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLLNMMK 1846 tr|A0A498LK69|A0A498LK69_LABRO CSNTF-RILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLLNMMK 1822 tr|A0A3Q2YTI5|A0A3Q2YTI5_HIPCM CSNTF-RILKSMVVGWVREITQYHNVYADNQVMHFYRWLHSPSSLLYDPALHRTLHNMMK 1847 tr|M4A042|M4A042_XIPMA CSNTF-RILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHNMMK 1847 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC CSNTF-RILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHNMMK 1846 tr|A0A3Q2PG22|A0A3Q2PG22_FUNHE CSNTF-RILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPTSLLYDPALHRTLHNMMK 1848 tr|A0A6A4SMP2|A0A6A4SMP2_SCOMX CSNTF-RILKSMVVGWVREITQFHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHNMMK 1825 tr|A0A673CZR7|A0A673CZR7_9TELE CSNTF-RILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHNMMK 1848 tr|A0A671YAK3|A0A671YAK3_SPAAU CSNTFSRILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHNMMK 1843 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE CSNTF-RILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPNSLLYDPALHRTLHNMMK 1846 tr|A0A7N8YN60|A0A7N8YN60_9TELE CSNTF-RILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHNMMK 1855 tr|A0A3P8N896|A0A3P8N896_ASTCA CSNTFSRILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHNMMK 1847 tr|A0A3B4GLI9|A0A3B4GLI9_9CICH CSNTFSRILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHNMMK 1847 tr|A0A6A5F6M3|A0A6A5F6M3_PERFL CSNTF-RILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHNMMK 1847 tr|A0A3Q1FLK4|A0A3Q1FLK4_9TELE CSNTF-RILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPNSLLYDPALHRTLHNMMK 1844 tr|A0A4W6G9K4|A0A4W6G9K4_LATCA CSNTF-RILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHNMMK 1847 tr|A0A3B4YTJ5|A0A3B4YTJ5_SERLL CSNTF-RILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHNMMK 1847 tr|A0A665X3K2|A0A665X3K2_ECHNA CSNTF-RILKSMVVGWVREITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHNMMK 1847 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE CSSTF-RILKSMVVGWVKEITQYGNVYADNQVVHFYRWLRSPSSLLHDPALHRTLHGWMK 1819 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA CSSTF-RVLKSMVVGWVKEITQYRNVYADNQVMHFYRWLRSPASLLHDPALHRTLHSMMK 1838 tr|A0A7J8H4F1|A0A7J8H4F1_ROUAE CSSTF-RVLKSMVVGWVKEITQYRNVYADNQVMHFYRWLRSPASLLHDPALHRTLHGMMK 1852 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF CSSTF-RILKSMVVGWVKEITQYRNIYADNQVMHFYRWLRSPSSLLHDPVLHRTLHNMMK 1881 sp|Q07864|DPOE1_HUMAN CSNTF-RILKSMVVGWVKEITQYHNIYADNQVMHFYRWLRSPSSLLHDPALHRTLHNMMK 1846 tr|A0A2I3S482|A0A2I3S482_PANTR CSNTF-RILKSMVVGWVKEITQYHNIYADNQVMHFYRWLRSPSSLLHDPALHRTLHNMMK 1846 tr|A0A2K6CI58|A0A2K6CI58_MACNE CSNTF-RILKSMVVGWVKEITQYHNIYADNQVMHFYRWLRSPSSLLHDPALHRTLHNMMK 1823 tr|A0A834DIT3|A0A834DIT3_9CHIR CSSTF-RILKSMVVGWVREITQYRNVYADNQVMHFYRWLRSPSSLLHDPALHRTLHNMMK 1845 tr|F6Z911|F6Z911_HORSE CSSTF-RILKSMVVGWVREITQYRNIYADNQVVHFYRWLRSPSSLLHDPALHRTLHNMMK 1846 tr|A0A5G2RB90|A0A5G2RB90_PIG CSSTF-RILKSMVVGWVKEITQYRNVYADNQVMHFYRWLRSPSSLLHDPALHRTLHGMMK 1819 tr|A0A670JP16|A0A670JP16_PODMU CSNTF-RVLKSVVVGWVKEITQYHNVYADNQVIHFYRWLRSPTSLLYDPALHRMLHSMMK 1842 tr|A0A852LC41|A0A852LC41_UROIN CSNTF-RILKSMVVSWVKEITQYHNVYADNQVIHFYRWLRSPSSLLYDPALHRTLHNMMK 1863 tr|F6ZFB6|F6ZFB6_ORNAN CSNTF-RILKSMVVGWVKEIMQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLHNMMK 1721 tr|A0A6P5LNS3|A0A6P5LNS3_PHACI CSNTF-RILKSMVVGWVKEITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLLSMMK 1847 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR CSNTF-RILKSMVVGWVKEITQYHNVYADNQVMHFYRWLRSPSSLLYDPALHRTLLSMMK 1831 **.** *:***:**.**:** :: *:******:******:** ***:**.*** * . ** ZBM-CTD-Pol tr|A0A6Q2WWN8|A0A6Q2WWN8_ESOLU KVFLQLVAEFKRLGSTVVYGNFNRILLCTKKRRIDDAIGYVEYITNSIHSREIFHSLSIS 1904 tr|A0A673YLH4|A0A673YLH4_SALTR KVFLQLVAEFKRLGSTVVYGNFNRILLCTKKRRIDDAIGYVEYITNSIHSREIFHSLSIS 1910 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE KVFLQLVAEFKRLGSTVVYGNFNRILLCTKKRRIDDAIGYVEYITNSIHSREIFHSLSIS 1908 tr|A0A6P6MI98|A0A6P6MI98_CARAU KIFLQLVAEFKRLGSSVIYGNFNRLILCTKKRRIDDAIAYVEYITNSIHSKEIFHSLSIT 1906 tr|A0A498LK69|A0A498LK69_LABRO KIFLQLVAEFKRLGSSVIYGNFNRLILCTKKRRIDDAIAYVEYITNSIHSKEIFHSLSIT 1882 tr|A0A3Q2YTI5|A0A3Q2YTI5_HIPCM KVFLQLIAEFKRLGSTVVYGNFNRIILCTKKRRIDDAIGYVEYITNSIHSREIFHSLSIS 1907 tr|M4A042|M4A042_XIPMA KVFLHAKTYLKV-G------YFCFLIS-----VFKQWKSLSICFSSSIHSREIFHSLSIS 1895 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC KVFLQLVSEFKRLGSTVVYGNFNRIMLCTKKRRIDDAISYVEYITNSIHSREIFHSLSIS 1906 tr|A0A3Q2PG22|A0A3Q2PG22_FUNHE KVFLQLVAEFKRLGSTVVYGNFNRIILCTKKRRIDDAIAYVDYITNSIHSKEIFHSLSIS 1908 tr|A0A6A4SMP2|A0A6A4SMP2_SCOMX KVFLQLVAEFKRLGSTVVYGNFNRIILCTKKRRIDDAIGYVEYITSSIHTKEIFHSLSLS 1885 tr|A0A673CZR7|A0A673CZR7_9TELE KVFLQLIAEFKRLGSTVVYGNFNRIILCTKKRRIDNAIGYIEYITNSIHSREIFHSLSIS 1908 tr|A0A671YAK3|A0A671YAK3_SPAAU KVFLQLISEFKRLGSTVVYGNFNRIILCTKKRRIDDAIGYVEYITNSIHSREIFHSLSIS 1903 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE KVFLQLIAEFKRLGSTVVYGNFNRIILCTKKRRIDDAIGYVEYITKSIHTREIFHSLSIS 1906 tr|A0A7N8YN60|A0A7N8YN60_9TELE KVFLQLVAEFKRLGSTVVYGNFNRIILCTKKRRTDDAIGYMDYITNSIHSKEIFHSVSIS 1915 tr|A0A3P8N896|A0A3P8N896_ASTCA KVFLQLVAEFKRLGSTVVYGNFNRIILCTKKRRIDDAIGYVEYITNSIHSKEIFHSLSIS 1907 tr|A0A3B4GLI9|A0A3B4GLI9_9CICH KVFLQLVAEFKRLGSTVVYGNFNRIILCTKKRRIDDAIGYVEYITNSIHSKEIFHSLSIS 1907 tr|A0A6A5F6M3|A0A6A5F6M3_PERFL KVFLQLVSEFKRLGSTVVYGNFNRIMLCTKKRRIDDAIGYVEYITKSIHSREIFHSLSIS 1907 tr|A0A3Q1FLK4|A0A3Q1FLK4_9TELE KVFLQLVAEFKRLGSTVVYGNFNRIILCTKKRRIDDAIGYVEYITNSIHSREIFHSLSIS 1904 tr|A0A4W6G9K4|A0A4W6G9K4_LATCA KVFLQLVAEFKRLGSTVVYGNFNRMILCTKKRRIDDAIGYVEYITNSIHSREIFHSLSMS 1907 tr|A0A3B4YTJ5|A0A3B4YTJ5_SERLL KVFLQLVAEFKRLGSTVVYGNFNRIILCTKKRRIDNAIGYVEYITNSIHSREIFHSLSLS 1907 tr|A0A665X3K2|A0A665X3K2_ECHNA KVFLQLVAEFKRLGSTVVYGNFNRIILCTKKRRTDDAIGYIEYITKSIHSREMFHSLSLS 1907 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE KLFLQLIAEFKRLGSSVVYANFNRIVLCTRKRRVEDALAYVEYITSSIHSKEIFHSLTIS 1879 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA KLFLQLIAKFKRLGSSVVYANFNRVVLCTKKQRVEDAVAYVEYITSSIHSKEIFHSLTIS 1898 tr|A0A7J8H4F1|A0A7J8H4F1_ROUAE KLFLQLIAEFKRLGSSVVYANFNRVVLCTKKRRVEDAIAYAEYITSSIHSKEIFHSLTIS 1912 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF KLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRIEDAIAYMEYITSSIHSKEIFHSLTIS 1941 sp|Q07864|DPOE1_HUMAN KLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRVEDAIAYVEYITSSIHSKETFHSLTIS 1906 tr|A0A2I3S482|A0A2I3S482_PANTR KLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRVEDAIAYVEYITSSIHSKEIFHSLTIS 1906 tr|A0A2K6CI58|A0A2K6CI58_MACNE KLFLQLITEFKRLGSSVVYANFNRIILCTKKRRVEDAIAYVEYITSSIHAKEIFHSLTIS 1883 tr|A0A834DIT3|A0A834DIT3_9CHIR KLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRIEDALAYVEYITTSIHSKEIFHSLTIS 1905 tr|F6Z911|F6Z911_HORSE KLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRIDDAIAYVDYITNSIHSKEIFHSLTIS 1906 tr|A0A5G2RB90|A0A5G2RB90_PIG KLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRVEDALAYVEYITNSIHSKEIFHSLTIS 1879 tr|A0A670JP16|A0A670JP16_PODMU KLFLQLVAEFKRLGSSVVYANFNRIILCTKKRRIEDALAYVEYITNSIHSREIFHSLTIS 1902 tr|A0A852LC41|A0A852LC41_UROIN KLFLQLVAEFKRLGSSVVYANFNRIILCTRKRRIEDALSYMHYIISSIHSKEIFHSLTIS 1923 tr|F6ZFB6|F6ZFB6_ORNAN KLFLQLVAEFKRLGSSVIYANFNRIILCTKKRRIEDALAYVDYITNSIHSKEIFHSLTIS 1781 tr|A0A6P5LNS3|A0A6P5LNS3_PHACI KLFLQLVAEFKRLGSSVIYANFNRIILCTKKRRIEDAITYIDYITNSIHSKEIFHSLTIS 1907 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR KLFLQLVAEFKRLGSSVIYANFNRIILCTKKRRIEDAIAYIDYITNSIHSKEIFHSLTIS 1891 *:**: : :* * * :: .: : .***::* ***:::: World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 563 tr|A0A6Q2WWN8|A0A6Q2WWN8_ESOLU FSRCWEFLMWMDPSNHGGVKGRLPSSVLYGETAQK--KK--GTGDEEGSEDEDEA---DG 1957 tr|A0A673YLH4|A0A673YLH4_SALTR FSRCWEFLMWMDLANYGGVKGKLPSSVLYGEVPSSSAKY--EDGEEEGSEDEEEA---DE 1965 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE FSRCWEFLMWMDLANYGGVKGKLPSSILYGEVPSSS------DGDEEGSEDEEEA---D1958 tr|A0A6P6MI98|A0A6P6MI98_CARAU FSRCWEFLLWMDPTNYGGVKGKLPSSLLYGEDGNEKKKKKNVEE-EDGSEEEE--DDEEN 1963 tr|A0A498LK69|A0A498LK69_LABRO FSRCWEFLLWMDPANCGGVKGKLPSSLLYGEHANEKKKNKDGEE-EDGSEDED--EDEVN 1939 tr|A0A3Q2YTI5|A0A3Q2YTI5_HIPCM FSQCWEFLLWMDPANNGGVKGKLPSSVLYGEVVQT---------------DEDEEEDEAN 1952 tr|M4A042|M4A042_XIPMA FSRCWEFLLWMDPSNYGGVKGKLPSSILYGEEGAKQKKRKQGEEDEEGSEDEDE----DD 1951 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC FSRCWEFLMWMDPSNFSGVKGKLPSSLMSGEDGAKQKKKSRGEGDEEGSEDEEE----DP 1962 tr|A0A3Q2PG22|A0A3Q2PG22_FUNHE FSRCWEFLMWMDPANYGGVKGKLPSSILYGEKKRK-----QGEEDEEGSE----DEDEAD 1959 tr|A0A6A4SMP2|A0A6A4SMP2_SCOMX FSRCWEFLLWMDPANYGGVKGKLPSNVLYGEEGGKRKKSRRGEGDEDGSEDE----DEAN 1941 tr|A0A673CZR7|A0A673CZR7_9TELE FSRCWEFLMWMDPANYGGVKGKLPSSVLYGERKK----TQQQEGAEDGSEDEEEDEEDAR 1964 tr|A0A671YAK3|A0A671YAK3_SPAAU FSRCWEFLLWMDPANCGGVKGKLPSR-------AKQKKNKRGEGEEEGSEDEDEEE---A 1953 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE FSRCWEFLMWMDPANYGGVKGKLPSSILYGEEGAKRKKNRQEEEDEDGSEDEDED----N 1962 tr|A0A7N8YN60|A0A7N8YN60_9TELE FSRCWEFLLWMDPANYSGVKGKLPPSLLYGEEGAKQKKNRQGEGHEEGSDDEDE--DEAD 1973 tr|A0A3P8N896|A0A3P8N896_ASTCA FSRCWEFLLWMDPANYGGVKGKLPSSVMYGEKKTR-----QEEGDEDGSE----DEDEEN 1958 tr|A0A3B4GLI9|A0A3B4GLI9_9CICH FSRCWEFLLWMDPANYGGVKGKLPSSVMYGEKKTR-----QEEGDEDGSE----DEDEEN 1958 tr|A0A6A5F6M3|A0A6A5F6M3_PERFL FSRCWEFLLWMDPANYGGVKGKLPSSIMYGEEGAQQKKKSRGEGDEEGSDDEDE--DEAV 1965 tr|A0A3Q1FLK4|A0A3Q1FLK4_9TELE FSRCWEFLLWMDPANYGGVKGKLPSSILYGEDGAKQKKNRQGEGDEDGSEDED----EED 1960 tr|A0A4W6G9K4|A0A4W6G9K4_LATCA FSRCWEFLMWMDPANYGGVKGKLPSSVLYGEGAKQRKNKRE--GDEDGSED--EDEDEAG 1963 tr|A0A3B4YTJ5|A0A3B4YTJ5_SERLL FSRCWEFLLWMDPANYGGVKGKLPSSLLYGEEGTKKKKNRQGDGDEDGSEDEDE--DEAD 1965 tr|A0A665X3K2|A0A665X3K2_ECHNA FSRCWEFLLWMDPANHGGVKGKLPSSLLYGEERPKKKKNGQGEDDQDGSEDEDEDEDEAD 1967 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE FSRCWEFLLWMDPSNYGGIKGKVPSGIHCGQRDSPKEGSTQGDE---DDEDD-------- 1928 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA FSRCWEFLLWMDPSNYGGIKGNVPSSIHCGQDSQNEGKEQDDED---GDEDG-------- 1947 tr|A0A7J8H4F1|A0A7J8H4F1_ROUAE FSRCWEFLLWMDPSNYGGIKGNVPSSVHCGQDPQNEGKEQDDED---GD--G-------- 1959 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF FSRCWEFLLWMDPSNYGGIKGNAPSSIHYGQQDSQKGGRVEEEE---EEEEE--EE---- 1992 sp|Q07864|DPOE1_HUMAN FSRCWEFLLWMDPSNYGGIKGKVSSRIHCGLQDSQKAGGAEDEQ---ENEDD--EEER-- 1959 CTD-Pol tr|A0A2I3S482|A0A2I3S482_PANTR FSRCWEFLLWMDPSNYGGIKGKVSSRIHCGLQDSQKAGGAEDEQ---ENEDD--EEER-- 1959 tr|A0A2K6CI58|A0A2K6CI58_MACNE FSRCWEFLLWMDPSNYGGIKGRVSSRVHHGLQDSQKVQGAEDEQ---ENEDN--EEER-- 1936 tr|A0A834DIT3|A0A834DIT3_9CHIR FSRCWEFLLWMDPSNFGGIKGNVPSRIHCGQQDSPKGGRAEEEE---EEEEE--EEEPEG 1960 tr|F6Z911|F6Z911_HORSE FSRCWEFLLWMDPCNYGGIKGNVPSSIHCGQPDSQKVGRVEGEE---EE--E--EEEPEE 1959 tr|A0A5G2RB90|A0A5G2RB90_PIG FSRCWELLLWMDPSNYGGIKGNVPSRIHCGQQDSPKEGRVEEEE---AE--E--EE--EE 1930 tr|A0A670JP16|A0A670JP16_PODMU FSRCWEFLLWMDPANYGGIKGKVPSQIHYGEVRKPAKKAEGEE-----------EEE-EE 1950 tr|A0A852LC41|A0A852LC41_UROIN FSRCWEFLLWMDPANYGGIKGKVDSRVHYGEEDTSKRQEVEEEE---DSEEE--EE---- 1974 tr|F6ZFB6|F6ZFB6_ORNAN FSRCWEFLLWMDPSNYGGIKGKVPSSVHYGERDTKKRQKAEEEE---GSDDE--EEE-E1834 tr|A0A6P5LNS3|A0A6P5LNS3_PHACI FSRCWEFLLWMDPANYGGIKGKVPNRVHYGEQSTQKEQKAEEEQ---ESEEE--EEE-EE 1961 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR FSRCWEFLLWMDPANYGGIKGKVPTHVHYGEVSSKQK--AEEEQ---ES-----EEE-EE 1940 **:***:*:*** * .*:**. CysA tr|A0A6Q2WWN8|A0A6Q2WWN8_ESOLU LVDVGEFSEDAQFRDPCNSYILPEVICHHCNFCRDLDLCK---DPSVAQDGSVLPQWFCS 2186 tr|A0A673YLH4|A0A673YLH4_SALTR LVDVGEFSEEAQFRDPCNSYILPEVICHQCNFCRDLDLCK---DPSVAQDGSVLPQWFCS 2193 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE LVDVGEFSEDAQFRDPCNSYILPEVICHHCNFCRDLDLCK---DPSVAQDGSVLPQWFCS 2186 tr|A0A6P6MI98|A0A6P6MI98_CARAU LVDVGEFSEDAQFRDPCKSYILPEVICHQCNFCRDLDLCK---DPAVSQDGSVLPQWFCS 2192 tr|A0A498LK69|A0A498LK69_LABRO LVDVGEFSEDAQFHDPCKSYVLPEVICHHCNFCRDLDLCK---DPAVSQDGSVLPQWFCS 2168 tr|A0A3Q2YTI5|A0A3Q2YTI5_HIPCM LVDVGEFSEEATFRDPCNSYILPEVICHNCNFCRDLDLCK---DPSVVQDGAALPQWFCS 2181 tr|M4A042|M4A042_XIPMA LVDVGEFSEEAQFRDPCNSYILPEVICHHCNFCRDLDLCK---DPTVAQDGSVLPQWFCS 2180 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC LVDVGEFSMQAQFSDPCNSYILPEVICHQCNFCRDLDLCK---DPSVAQDGSVLPQWFCS 2191 tr|A0A3Q2PG22|A0A3Q2PG22_FUNHE ------------------------------------------------------------ 2016 tr|A0A6A4SMP2|A0A6A4SMP2_SCOMX LVDVGEFSENAQFQDPCNSYILPEVICHHCNFCRDLDLCK---DPSVAQDGSVLPQWFCS 2170 tr|A0A673CZR7|A0A673CZR7_9TELE LVDVGEFSEEAQFRDPCNSYILPEVICHHCNFCRDLDLCK---DPSMAQDGSVLPQWFCS 2193 tr|A0A671YAK3|A0A671YAK3_SPAAU LVDVGEFSDDAQFRDPCNSYILPEVICHHCNFCRDLDLCK---DPSVAQDGSVLPQWFCS 2182 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE LVDVGEFSEDAQFRDPCNSYILPEVICHHCNFCRDLDLCK---DPSMAQDGSVLPQWFCS 2191 tr|A0A7N8YN60|A0A7N8YN60_9TELE LVDVGEFSEDAQFRDPCNSYILPEVICHHCNFCRDLDLCK---DPSVAQDGSVLPQWFCS 2202 tr|A0A3P8N896|A0A3P8N896_ASTCA ------------------------------------------------------------ 2036 tr|A0A3B4GLI9|A0A3B4GLI9_9CICH ------------------------------------------------------------ 2015 tr|A0A6A5F6M3|A0A6A5F6M3_PERFL LVDVGEFSGEAQFCDPCNSYILPEVICHHCNFCRDLDLCR---DPSVAQDGSVLPQWFCS 2194 tr|A0A3Q1FLK4|A0A3Q1FLK4_9TELE ------------------------------------------------------------ 2010 tr|A0A4W6G9K4|A0A4W6G9K4_LATCA LVDVGEFSDDAQFRDPCNSYILPEVICHHCNFCRDLDLSALLNSNSSNIDGSVLPQWFCS 2195 tr|A0A3B4YTJ5|A0A3B4YTJ5_SERLL LVDVGEFSEDAQFRDPCNSYILPEVICHQCNFCRDLDLCK---DPSVAQDGSVLPQWFCS 2194 tr|A0A665X3K2|A0A665X3K2_ECHNA LVDVGEFSEDAQFRDPCNSYILPEVICHHCNFCRDLDLCK---DPSVAQDGSVLPQWFCS 2196 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE LVDVGEFSAEAQFRDPCRSYVLPEVICHSCHFCRDLDLCK---DPFVSQDGAMPPRWLCS 2157 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA LVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCQDLDLCR---DPSVSQDGAVLPQWLCS 2184 tr|A0A7J8H4F1|A0A7J8H4F1_ROUAE LVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCQDLDLCR---DPSVSQDGAVLPQWLCS 2188 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF LVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCK---EFAFSQDGAVLPQWLCS 2174 sp|Q07864|DPOE1_HUMAN LVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCK---DSSFSEDGAVLPQWLCS 2188 tr|A0A2I3S482|A0A2I3S482_PANTR LVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCK---DSSFSEDGAVLPQWLCS 2188 tr|A0A2K6CI58|A0A2K6CI58_MACNE LVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCK---DSSFSEDGAVLPQWLCS 2165 tr|A0A834DIT3|A0A834DIT3_9CHIR LVDVGEFSEEAQFRDPCHSYVLPEVICHSCNFCRDLDLCK---EPSFSQDGAMLPQWLCS 2189 tr|F6Z911|F6Z911_HORSE LVDVGEFSEEAQFQDPCRSYVLPEVICHSCNFCRDLDLCK---DPSFSQDGAVLPQWLCS 2188 tr|A0A5G2RB90|A0A5G2RB90_PIG LVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCK---EPSFSQDGAVLPQWLCS 2159 tr|A0A670JP16|A0A670JP16_PODMU LIEVGEFSEEAQFRDPCRSYVIPEMICRNCNFCRDLDLCK---DPALSQDGSVLPTWACS 2179 tr|A0A852LC41|A0A852LC41_UROIN LAEVGEFSDEAQFRDPCRSYVLPEVICRNCNFCRDLDLCK---DPALSQDGSVLPGWVCS 2202 tr|F6ZFB6|F6ZFB6_ORNAN LVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCK---DPSVSQDGSVLPQWLCS 2061 tr|A0A6P5LNS3|A0A6P5LNS3_PHACI LVDVGEFSEEARFRDPCRSYVLPEVICRSCNFCRDLDLCK---DPFLSQDVSVLPQWLCS 2190 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR LVDVGEFSEEAQFRDPCRSFVLPEVICRNCNFCRDLDLCK---DPFLSQDVSILPQWLCS 2169 CysA 4Fe-4S tr|A0A6Q2WWN8|A0A6Q2WWN8_ESOLU NCQAQYETESIEMALVEALQKKLMSYTLQDLACSKCKGVKEANMPLYCTCAGDFELTFPT 2246 tr|A0A673YLH4|A0A673YLH4_SALTR NCQTQYETESIEMALVEALQKKLMSYTLQDLACAKCKGVKEANMPLYCTCAGDFELTFPT 2253 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE NCQTQYETESIEMALVEALQKKLMSYTLQDLACAKCKGVKEANMPLYCTCAGDFELTFPT 2246 tr|A0A6P6MI98|A0A6P6MI98_CARAU NCQAQYDTESIEMALVEALQKKLMSYTLQDLECSKCKGVKEANMPLYCTCAGDFNLSFTT 2252 tr|A0A498LK69|A0A498LK69_LABRO NCQAQYDTESIEMALVEALQKKLMSYTLQDL----------------------------- 2199 tr|A0A3Q2YTI5|A0A3Q2YTI5_HIPCM NCQAQYETESIEMALVEALQKKLMSYTLQDLVCTKCKGVKEANMPLYCKCAGDFELTFPA 2241 tr|M4A042|M4A042_XIPMA NCQMQYETESIETALVDALQKKLMSYTLQDLVCTKCKGVKEANMPLYCACAGNFDLTFSA 2240 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC NCQTQYDTESIEMALVEALQKKMMSYTLQDLVCTKCKGVKEANMPLYCKCAGDFILTFSA 2251 tr|A0A3Q2PG22|A0A3Q2PG22_FUNHE ------------------------------------------------------------ 2016 tr|A0A6A4SMP2|A0A6A4SMP2_SCOMX NCQAQYETESIEMALVEALQKKLMSYTLQDL----------------------------- 2201 tr|A0A673CZR7|A0A673CZR7_9TELE NCQAQYETESIEMALVEALQKKLMSYTLQDLVCTKCKGVKEANMPLYCSCAGDFDLTFSA 2253 tr|A0A671YAK3|A0A671YAK3_SPAAU NCQAQYETESIEMALVEALQKKLMSYTLQDLVCNKCKGVKEANMPLYCRCAGDFELTFPA 2242 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE NCQAPYETDSIEMSLVEALQKKLMSYTLQDLACNKCKGVKEANMPLYCRCAGDFELTFSA 2251 tr|A0A7N8YN60|A0A7N8YN60_9TELE NCQAQYETESIEMALVEALQKKLMSYTLQDLVCTKCKGVKEANMPLYCGCAGNFDLTFSA 2262 tr|A0A3P8N896|A0A3P8N896_ASTCA ------------------------------------------------------------ 2036 tr|A0A3B4GLI9|A0A3B4GLI9_9CICH ------------------------------------------------------------ 2015 tr|A0A6A5F6M3|A0A6A5F6M3_PERFL NCQAQYETESIEMALVEALKKKLMSYTLQDLVCTKCKGVKEANMPLYCKCAGDFDLTFPA 2254 tr|A0A3Q1FLK4|A0A3Q1FLK4_9TELE ------------------------------------------------------------ 2010 tr|A0A4W6G9K4|A0A4W6G9K4_LATCA NCQAQYETETIEMALVEALQKKLMSYTLQDLVCTKCKGVKEANMPLYCGCAGDFDLSFSA 2255 tr|A0A3B4YTJ5|A0A3B4YTJ5_SERLL NCQAQYETESVEMALVEALQKKLMSYTLQDLVCTKCKGVKEANMPLYCGCAGDFDLTFSA 2254 tr|A0A665X3K2|A0A665X3K2_ECHNA NCQAQYETESVEMALVEALQKKLMSYTLQDLVCVKCKGVKEANMPLYCGCAGDFDLTFSA 2256 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE NCQVAYDSSVIEMALVAALQKKLMAFTLQDLVCLKCRGVKETHMPTYCSCAGGFSLTIHT 2217 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA NCQAAYDSSVIEAALVEALQKKLMAFTLQDLACLRCGGVKETHMSVYCTCAGNFALTIRT 2244 tr|A0A7J8H4F1|A0A7J8H4F1_ROUAE NCQAAYDSSVIEAALVEALQKKLMAFTLQDLACLRCRGVKETHMPVYCACAGSFALTIHT 2248 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF NCQVAYDTSVIEMALVEALQKKLMAFTLQDLICLKCRGVKETNMPVYCSCAGGFTLTIHT 2234 sp|Q07864|DPOE1_HUMAN NCQAPYDSSAIEMTLVEVLQKKLMAFTLQDLVCLKCRGVKETSMPVYCSCAGDFALTIHT 2248 tr|A0A2I3S482|A0A2I3S482_PANTR NCQAPYDSSAIEMTLVEVLQKKLMAFTLQDLVCLKCRGVKETSMPVYCSCAGDFALTIHT 2248 tr|A0A2K6CI58|A0A2K6CI58_MACNE NCQAPYDSSVIEMTLVEVLQKKLMAFTLQDLICLKCRGVKETSMLVYCSCAGDFTLTVHT 2225 tr|A0A834DIT3|A0A834DIT3_9CHIR NCQVAYDSSVIEMALVEALQKKLMAFTLQDLICLKCRGVKETNMAVYCSCAGDFALTIHT 2249 tr|F6Z911|F6Z911_HORSE NCQIAYDSSVIEMALVEALQKKLMAFTLQDLICLKCRGVKETNMPVYCDCAGGFTLTIHT 2248 tr|A0A5G2RB90|A0A5G2RB90_PIG NCQVAYDSSVIEMALVEALQKKLMAFTLQDLVCVKCRGVKETNMAVYCSCAGGFALTIHT 2219 tr|A0A670JP16|A0A670JP16_PODMU NCQAQYDSDAIEMALVEALQKKLMAFTLQDLVCLKCKGIKDTHMPVYCSCAGDFDLLLPT 2239 tr|A0A852LC41|A0A852LC41_UROIN SCQAQYDSGAIEAALLEALQKKLMAFVLQDLVCRKCHGVKDTHMPVYCSCAGDFALTISS 2262 tr|F6ZFB6|F6ZFB6_ORNAN NCQAAYDTDHIEMALVEVLQKKLMAFSLQDLVCLKCKGVKEANMPIYCSCAGDFSLTIPT 2121 tr|A0A6P5LNS3|A0A6P5LNS3_PHACI NCQAAYDSGHIEMALVEALQKKLMAFTLQDLICLKCKGVKEANMPIYCSCAGDFALTIQT 2250 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR NCQAAYDSGHIEMALVEALQKKLMAFTLQDLICLKCKGVKEANMPVYCSCAGDFALTIQT 2229 World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 564 Figure 4 MSA of ε DNA polymerases from various animal sources 3.2. ‘Mix and Match’ MSA analysis of the DNA pols ε from yeasts, higher fungi, plants and animals Figure 5 shows the ‘Mix and Match’ MSA of the ε pols from all the four eukaryotic sources, viz. yeasts, higher fungi, plants and animals (only the required regions for the discussions are shown). S. cerevisiae, Aspergillus niger, A. thaliana and human sequences are used as the standards and highlighted. All the major domains (NTD, PR Exo, Pol, Linker and CTD) are indicated tentatively with arrow marks. The NTDs show many gaps in the alignment, but with a small number of conserved peptides. Strikingly, a highly conserved dodecapeptide is found in this region. The NTD is followed by a highly conserved PR exo domain. The proposed PR exonuclease active site amino acids are completely conserved in all (highlighted) and it belongs to the DEDD-superfamily with an invariant Y as a proton acceptor. The presence of a typical –DEDD-superfamily exonuclease active site in all ε pols is in close agreement with the other DdDps [8]. An invariant, characteristic motif –SYLPQ/VGS- (highlighted in yellow) is found within the PR exo domain in the ε pols of all eukaryotes. This sequence is somewhat similar to the characteristic –SLYPSmotif reported in the other two replicative pols, α and δ [2] and, in general, in all the B-family pols. In all these pols, viz. ε, δ and α, this motif is implicated in dNTPbinding. //End of ε pol sequences from animal sources tr|A0A6Q2WWN8|A0A6Q2WWN8_ESOLU AAHYNMNFLEETIDWLLVMSPQISQAARH2288 tr|A0A673YLH4|A0A673YLH4_SALTR AAHYNMNFLEETIDWLLVMSPQINQSR--- 2293 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE AAHYNMNFLEETIDWLLVMSPQISQSR--- 2286 tr|A0A6P6MI98|A0A6P6MI98_CARAU AAHYNMNFLLETIDWVLSMNA--------- 2286 tr|A0A498LK69|A0A498LK69_LABRO AAHYNMNFLLETIDWVLSMNA--------- 2232 tr|A0A3Q2YTI5|A0A3Q2YTI5_HIPCM ASHYNMSFLKETIDWLMVMSPQISQSAS-- 2282 tr|M4A042|M4A042_XIPMA ASHYNMRFLGETIDWLLEMSPQIGQSRH-- 2281 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC ASHYSMSYLGETIDWLLVMSPQISQSAL-- 2292 tr|A0A3Q2PG22|A0A3Q2PG22_FUNHE ------------------------------ 2016 tr|A0A6A4SMP2|A0A6A4SMP2_SCOMX ASHYNMSFLEETIDWLLVMSPHVGQSIH-- 2236 tr|A0A673CZR7|A0A673CZR7_9TELE ASHYNMSFLEETIEWLRGMSPQINQSTHKI 2296 tr|A0A671YAK3|A0A671YAK3_SPAAU ASHYSMSFLEETIDWLVVMSPHINQSAR-- 2283 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE ASHYNMTFLEETIDWLLLMSPQISQSAR-- 2292 tr|A0A7N8YN60|A0A7N8YN60_9TELE ASHYNMRFLEETIDWLLVMSPQISQSAH-- 2303 tr|A0A3P8N896|A0A3P8N896_ASTCA ------------------------------ 2036 tr|A0A3B4GLI9|A0A3B4GLI9_9CICH ------------------------------ 2015 tr|A0A6A5F6M3|A0A6A5F6M3_PERFL ASHYKMSFLDETLDWLLVMSPQISQNAH-- 2295 tr|A0A3Q1FLK4|A0A3Q1FLK4_9TELE ------------------------------ 2010 tr|A0A4W6G9K4|A0A4W6G9K4_LATCA ASHYSMSFLEETIDWLLVMSPQISQSAR-- 2296 tr|A0A3B4YTJ5|A0A3B4YTJ5_SERLL ASHYNMSFLEETIDWLLAMSPQISQSAH-- 2295 tr|A0A665X3K2|A0A665X3K2_ECHNA ASHYNMSFLEETIDLLLAMSPQISQTAH-- 2297 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE AQHYGMSYLMETLEWLLEKNPQLGH----- 2255 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA AQHCGMSYLMETLEWLLQRNPQLGH----- 2282 tr|A0A7J8H4F1|A0A7J8H4F1_ROUAE AQHCGMSYLMETLEWLLQKNPQLGQ----- 2286 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF AQHYGMSYLTETLEWLLQKNPQLGH----- 2319 sp|Q07864|DPOE1_HUMAN AQHYGMSYLLETLEWLLQKNPQLGH----- 2286 tr|A0A2I3S482|A0A2I3S482_PANTR AQHYSMSYLLETLEWLLQKNPQLGR----- 2286 tr|A0A2K6CI58|A0A2K6CI58_MACNE AQHYGMSYLLETLEWLLQKNPQRGR----- 2263 tr|A0A834DIT3|A0A834DIT3_9CHIR AQHYGMSYLMETLEWLLQKNPQLSH----- 2287 tr|F6Z911|F6Z911_HORSE AQHYGMSYLMESLEWLLQKNPQLGH----- 2286 tr|A0A5G2RB90|A0A5G2RB90_PIG AQHYGMSYLTETLEWLLQQNPQLGH----- 2257 tr|A0A670JP16|A0A670JP16_PODMU ARHYNMDYLLENIKWLLQMNPQLLL----- 2277 tr|A0A852LC41|A0A852LC41_UROIN ARHYSMAHLLETIEWLLSTNPQLRP----- 2300 tr|F6ZFB6|F6ZFB6_ORNAN AQHYAMSYLLETIEWLLQMNPRLPR----- 2159 tr|A0A6P5LNS3|A0A6P5LNS3_PHACI ARHYGMTYLTEMIEWLLQMNPQLAN----- 2288 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR ARHYGMTYLTEMIEWLLQMNPQLAN----- 2267 Figure 4 MSA of DNA pols ε from animal sources A0A6Q2WWN8_ESOLU, Esox lucius A0A673YLH4_SALTR, Salmo trutta A0A4W5MAK2_9TELE, Hucho hucho A0A6P6MI98_CARAU, Carassius auratus A0A498LK69_LABRO, Labeo rohita A0A3Q2YTI5_HIPCM, Hippocampus comes M4A042_XIPMA, Xiphophorus maculates A0A6P8SZE0_GYMAC, Gymnodraco acuticeps A0A3Q2PG22_FUNHE, Fundulus heteroclitus A0A6A4SMP2_SCOMX, Scophthalmus maximus A0A673CZR7_9TELE, Sphaeramia orbicularis A0A671YAK3_SPAAU, Sparus aurata A0A6P7J2Q5_9TELE, Parambassis ranga A0A7N8YN60_9TELE, Mastacembelus armatus A0A3P8N896_ASTCA, Astatotilapia calliptera A0A3B4GLI9_9CICH, Pundamilia nyererei A0A6A5F6M3_PERFL, Perca fluviatilis A0A3Q1FLK4_9TELE, Acanthochromis polyacanthus A0A4W6G9K4_LATCA, Lates calcarifer A0A3B4YTJ5_SERLL, Seriola lalandi dorsalis A0A665X3K2_ECHNA, Echeneis naucrates A0A7J7RQ84_RHIFE, Rhinolophus ferrumequinum A0A6P6D0R3_PTEVA, Pteropus vampyrus A0A7J8H4F1_ROUAE, Rousettus aegyptiacus A0A5F4D2S5_CANLF, Canis lupus familiaris Q07864|DPOE1_HUMAN, Homo sapiens A0A2I3S482_PANTR, Pan troglodytes A0A2K6CI58_MACNE, Macaca nemestrina A0A834DIT3_9CHIR, Phyllostomus discolor F6Z911_HORSE, Equus caballus A0A5G2RB90_PIG, Sus scrofa A0A670JP16_PODMU, Podarcis muralis A0A852LC41_UROIN, Urocolius indicus F6ZFB6_ORNAN, Ornithorhynchus anatinus A0A6P5LNS3_PHACI, Phascolarctos cinereus A0A4X2K1K8_VOMUR, Vombatus ursinus World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 565 The PR exo domain is followed by the pol domain and is also highly conserved in all. Interestingly, the template-binding pair (-YG-), catalytic amino acid (K) and dNTP selection amino acid (Q) are completely conserved in all (highlighted in yellow) and are in close agreement with other DdDps and DdRps [6, 21]. The ε pol’s active site amino acids are found within highly conserved large peptides. The pol domain also contains the completely conserved–DxDtype catalytic Mg2+ site and is in close agreement with other DNA pols. A unique ZBM that is conserved within the pol domain in all ε pols (highlighted) is suggested to play a role in linking redox status of cells to replication. The pol domain is followed by a linker region which connects the pol to the CTD. The linker region contains a highly conserved NLS-like motif in all pols ε (highlighted). The linker region is not conserved much. The CTD possesses two invariant ZBMs and one of them binds to the 4Fe-4S cluster (highlighted in orange) and a –DxDtype metal-binding motif is found within the first ZBM. The second pol and PR exo active sites do not align in all. Interestingly, the catalytic amino acid K, pairs with –Cas -KCin plant and animal enzymes, but with -Vas–KVin yeasts and higher fungal enzymes. CLUSTAL O (1.2.4) ‘Mix and Match’ MSA of all the four eukaryotic ε DNA pols (from yeasts, higher fungi, plants and animals) tr|A0A3P5YCF9|A0A3P5YCF9_BRACM ILAGK-----------------REQRLQDCLDSLLDLREYDVPYHVRFAVDKDVRSGQWY 218 sp|F4HW04|DPOE1_ARATH ILAGK-----------------REQRPQDCLDSIVDLREYDVPYHVRFAIDNDVRSGQWY 218 tr|D7KI06|D7KI06_ARALL ILAGK-----------------REQRPQDCLDSIVDLREYDVPYHVRFAIDNDVRSGQWY 218 tr|A0A8I6WFC8|A0A8I6WFC8_HORVV IYGVK-----------------RVERPQDYINCIIDLREYDVPYHVRFAIDNDVRSGQWY 242 tr|A0A452YAR6|A0A452YAR6_AEGTS IYGVK-----------------RVERPQDYINCIIDLREYDVPYHVRFAIDNDVRSGQWY 182 tr|A0A3B5XXC3|A0A3B5XXC3_WHEAT IYGVK-----------------RVERPQDYINCIIDLREYDVPYHVRFAIDNDVRSGQWY 237 tr|A0A1D6H8Z0|A0A1D6H8Z0_MAIZE IHGVK-----------------RVERPQDYINYIIDLREYDVPYHVRFAIDNDVRSGQWY 234 tr|A0A835B357|A0A835B357_9POAL IHGVK-----------------RVERPQDYINYIIDLREYDVPYHVRFAIDNDVRCGQWY 228 tr|A0A2S3GPC0|A0A2S3GPC0_9POAL IHGVK-----------------RVERPQDYMNYIIDLREYDVPYHVRFAIDNDVRCGQWY 233 tr|A0A368PL62|A0A368PL62_SETIT IHGVK-----------------RVERPQDYINYIIELREYDVPYHVRFAIDKDVRCGQWY 237 tr|A0A7I8KHB8|A0A7I8KHB8_SPIIN IFTGK-----------------SKERPQDFIDCIVDLREYDVPYHVRFAIDNDVRCGEWY 219 tr|A0A6I9QIG1|A0A6I9QIG1_ELAGV IYNGK-----------------SKERPQDYIDCITDLREYDVPYHVRFAIDNDVRCGQWY 217 tr|A0A8B8JBC2|A0A8B8JBC2_PHODC MYNGK-----------------SKERPQDYIDCITDLREYDVPYHVRFAIDNDVRCGQWY 217 tr|A0A9Q0FIP5|A0A9Q0FIP5_9ROSI ILSGKR--QVFHDHMHELTHYFLEQRLQDFLDSIIDLREYDVPYHVRFAIDNDIRCGQWY 234 tr|A0A2C9VYJ6|A0A2C9VYJ6_MANES LLTGKS----------------REQRPQDLLDCIVDLREYDVPYHVRFAIDNDIRCGQWY 220 tr|A0A7J7C034|A0A7J7C034_TRIWF ILTGK-----------------REQRPQDFLDCVVDLREYDVPYHVRFAIDNDVRCGQWY 219 tr|A0A2N9F976|A0A2N9F976_FAGSY FDISV-----------------ENKGPQDFIDCIVDLREYDVPYHVRFAIDNDVRCGQWY 238 tr|A0A7N2KME6|A0A7N2KME6_QUELO IIAGK-----------------REQRPQDFLDCIVDLREYDVPYHVRFAIDNDVRCGQWY 218 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE MLSSALAGGSV--SAA--DEDGMSKKTSDQMDNIVDMREYDVPYHVRLSIDLKIHVAHWY 245 tr|A0A8C7Q9V4|A0A8C7Q9V4_ONCMY MLSSALAGGSV--SAA--DEDGLSKKTSDQMDNIVDMREYDVPYHVRLSIDLKIHVAHWY 203 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE MLSSALSGGTV--ASA--DEDSISKSISDQLDNIVDMREYDVPYHVRVSIDLKIHVAHWY 245 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC MLSSALSGGNV--NTA--EEDGPSKRISDQLDNIVDMREYDVPYHMRLSIDLKIHVAHWY 243 tr|A0A8J0QX44|A0A8J0QX44_XENTR MLSSALTGNNM--GA---EEEGPSKKISDQMENIVDMREYDVPYHVRVSIDLKIHVAHWY 242 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR MLSSALMGGSV--TT---DEEGSSKKIADQLDNVVDMREYDVPYHIRLSIDLKIHVAHWY 229 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF MLSSVLQGGSV--VI---DEEEASKKVTDQLDNIVDMREYDVPYHIRLSIDLKIHVAHWY 279 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE MLSSVLQGGSV--IL---DEEEASKKTADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWY 217 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA MLSSVLQGGGA--II---DEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWY 236 tr|A0A2K6CI58|A0A2K6CI58_MACNE LLSSVLQRGGV--IT---DEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWY 244 tr|F6QAQ1|F6QAQ1_MACMU LLSSVLQRGGV--IT---DEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWY 246 tr|G3RQP2|G3RQP2_GORGO LLSSVLQRGGV--IT---DEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWY 223 sp|Q07864|DPOE1_HUMAN LLSSVLQRGGV--IT---DEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWY 244 tr|A0A2I3S482|A0A2I3S482_PANTR LLSSVLQRGGV--IT---DEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWY 244 tr|F6Z911|F6Z911_HORSE MLSSVLQGGSV--II---DEEETSKKMADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWY 244 tr|A0A5G2RB90|A0A5G2RB90_PIG MLSSVLQGGSV--VM---DEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWY 217 tr|A0A420PL83|A0A420PL83_FUSOX ----NGDFDLFDDSRD--DDRNMNTALSDASDFIVDIREYDVPYHVRVMIDLDIRAGKWY 222 tr|A0A1V6PGE7|A0A1V6PGE7_PENDC MSSATASFDLFDDEII--NEQRPN-GNTQASDFIIDIREYDVPYHVRVCIDKDIRVGKWY 257 tr|A0A0G4NU82|A0A0G4NU82_PENCA MTSATAGIDLFDDEII--NEQRPN-GNLQASDFIVDIREYDVPYHVRVCIDKDIRIGKWY 257 tr|A0A1V6TET5|A0A1V6TET5_9EURO MTSATAGIDLFDDEII--NEQRPN-GNLQASDFIVDIREYDVPYHVRVCIDKDIRIGKWY 257 tr|A0A167WJ50|A0A167WJ50_PENCH MTSATAGIDLFDDEII--NEQRPN-GNLQASDFIVDIREYDVPYHVRVCIDKDIRIGKWY 257 tr|A0A3A2Z9P9|A0A3A2Z9P9_9EURO ISSANAGFDLFDDELI--NESRTN-NSMNASDFIVDIREYDVPYHVRVAIDKDIRIGKWY 257 tr|A0A401L0U3|A0A401L0U3_ASPAW ISSANAGFDLFDDELI--NESRSN-ATMNASDFIIDIREYDVPYHVRVSIDKDIRIGKWY 257 tr|A0A317VH96|A0A317VH96_ASPEC ISSANAGFDLFDDELI--NESRSN-ATMNASDFIIDIREYDVPYHVRVSIDK----GKWY 253 tr|A0A117E1M7|A0A117E1M7_ASPNG ISSANAGFDLFDDELI--NESRSN-ATMNASDFIIDIREYDVPYHVRVSIDKDIRIGKWY 257 tr|A0A1D8PTD0|A0A1D8PTD0_CANAL KEI---------------------ETIMDPSVYIDDIREYDVPYHVRVSIDRNVRVGKWY 263 tr|C5M3R9|C5M3R9_CANTT KDV---------------------DSSSDPSTYIDDIREYDVPYHVRVSIDKQLRVGKWY 258 sp|Q6FNY7|DPOE_CANGA SNISNGNGMLSSNVNR--FASSSVQDKKDAKQYIEDIREYDVPYHVRVSIDKDIRVGKWY 265 sp|P21951|DPOE_YEAST AAN--------------------GSEKVDAKHLIEDIREYDVPYHVRVSIDKDIRVGKWY 205 tr|A0A7H9HLU1|A0A7H9HLU1_9SACH QGA--------------------GNSFRDAKSLIEEVREYDVPYHVRVSIDKNIRVGKWY 255 sp|Q6CUS7|DPOE_KLULA GSD---------------------YSTRDVKTLIEDIREYDVPYHVRVSIDKNIRVGKWY 182 sp|Q752B8|DPOE_ASHGO NDT---------------------TNRRDAKMLIDDIREYDVPYHVRVCIDKEIRVGKWY 179 : : ::********:*. :* ..** World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 566 NTD PR exo tr|A0A3P5YCF9|A0A3P5YCF9_BRACM NVSISSTD—VTLEKRTDILQR AEVRVCAFDIETTKLELKFPDAEYDQIMMISYMVDGQG 276 sp|F4HW04|DPOE1_ARATH NVSISSTD—VILEKRTDLLQR AEVRVCAFDIETTKLPLKFPDAEYDQIMMISYMVDGQG 276 tr|D7KI06|D7KI06_ARALL NVSISSTD—VILEKRTDLLQR AEVRVCAFDIETTKLPLKFPDAEYDQIMMISYMVDGQG 276 tr|A0A8I6WFC8|A0A8I6WFC8_HORVV NVGVSGSD—VLLQRREDLLQR AEVHVCAFDIETTKLPLKFPDAEYDSVMMISYMIDGQG 300 tr|A0A452YAR6|A0A452YAR6_AEGTS NVGVSGSD—VLLQRREDLLQR AEVHVCAFDIETTKLPLKFPDAEYDSVMMISYMIDGQG 240 tr|A0A3B5XXC3|A0A3B5XXC3_WHEAT NVGVSGSD—VLLQRREDLLQR AEVHVCAFDIETTKLPLKFPDAEYDSVMMISYMIDGQG 295 tr|A0A1D6H8Z0|A0A1D6H8Z0_MAIZE NVNVSGSD—VLLQRREDLLQR AEVHVCAFDIETTKLPLKFPDAEYD------------- 279 tr|A0A835B357|A0A835B357_9POAL NVSVSSSD—VLLQRREDLLQR AEVHVCAFDIETTKLPLKFPDAEYDTVMMISYMIDGQG 286 tr|A0A2S3GPC0|A0A2S3GPC0_9POAL NVSVSGSD—VLLQRREDLLQR AEVHVCAFDIETTKLPLKFPDAEYDTVMMISYMIDGQG 291 tr|A0A368PL62|A0A368PL62_SETIT NVSVSGSD—ALLQRREDLLQR AEVHVCAFDIETTKLPLKFPDAEYDTVMMISYMIDGQG 295 tr|A0A7I8KHB8|A0A7I8KHB8_SPIIN DVSISGIN—VLLQKRNDLLQR AEVHVCAFDIETTKLPLKFPDAEYDQVMMISYMVDGHG 277 tr|A0A6I9QIG1|A0A6I9QIG1_ELAGV DVNVSSAG—ILLEKRADLLQR AEVHVCAFDIETTKLPLKFPDAEYDLIMMISYMIDGQG 275 tr|A0A8B8JBC2|A0A8B8JBC2_PHODC DINVSSSG—ILLDKRTDLLQR AEVHVCAFDIETTKLPLKFPDAEYDLIMMISYMIDGQG 275 tr|A0A9Q0FIP5|A0A9Q0FIP5_9ROSI DVSVSSTG—VMLEKRTDLLQR AEVHICAFDIETTKLPLKFPDADYDQVMMISYMVDGQG 292 tr|A0A2C9VYJ6|A0A2C9VYJ6_MANES DVTVSNRG—VTLEKRTDLLQR AEVRVCAFDIETTKLPLKFPDADYDLIMMISYMVDGQG 278 tr|A0A7J7C034|A0A7J7C034_TRIWF DVSVSSNG—VVLEKRTDLLQR AEVHVCAFDIETTKLPLKFPDAEYDLVMMISYMVDGQG 277 tr|A0A2N9F976|A0A2N9F976_FAGSY DVSVSSTG—LMLGKRTDLLQR AEVHVCAFDIETTKLPLKFPDAEYDLIMMISYMVDGQG 296 tr|A0A7N2KME6|A0A7N2KME6_QUELO DVSVSSTG—LMLEKRTDLLQR AEVHVCAFDIETTKLPLKFPDAEYDLIMMISYMVDGQG 276 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE NVRYRGSAYPPEIILRSDLVER PDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQG 305 tr|A0A8C7Q9V4|A0A8C7Q9V4_ONCMY NVRYRGSAYPPEIILRSDLVER PDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQG 263 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE NVRFRGSAYPPEIVRRDDLVER PDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQG 305 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC NVRYRGSAYHPEIIRRDDLVER PDPVVLAYDIETTKLPLKFPDAESDQIMMISYMIDGQG 303 tr|A0A8J0QX44|A0A8J0QX44_XENTR NIRYRGSSSPPEITRRDDLVER PDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQG 302 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR NVRYRGSSLPVEITRRDDLVER PDPVVLAFDIETTKLPLKFPDAETDQIMMISYMVDGQG 289 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF NIRYRGSAFPVEITRRDDLVER PDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQG 339 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE NVRYRGNAFPVEVTRRDDLVER PDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQG 277 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA NVRFRGNAFPVELTRRDDLVER PDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQG 296 tr|A0A2K6CI58|A0A2K6CI58_MACNE NVRYRGNAFPVEITRRDDLVER PDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQG 304 tr|F6QAQ1|F6QAQ1_MACMU NVRYRGNAFPVEITRRDDLVER PDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQG 306 tr|G3RQP2|G3RQP2_GORGO NVRYRGNAFPVEITRRDDLVER PDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQG 283 sp|Q07864|DPOE1_HUMAN NVRYRGNAFPVEITRRDDLVER PDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQG 304 tr|A0A2I3S482|A0A2I3S482_PANTR NVRYRGNAFPVEITRRDDLVER PDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQG 304 tr|F6Z911|F6Z911_HORSE NVRYRGNAFPVEIARRDDLVER PDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQG 304 tr|A0A5G2RB90|A0A5G2RB90_PIG NVRYRGNAFPVEIARRDDLVER PDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQG 277 tr|A0A420PL83|A0A420PL83_FUSOX FVEAKHGV—TKIYPNEERSLP AEPVVMAYDIETSKLPLKFPDAATDQIIMISYMIDGQG 280 tr|A0A1V6PGE7|A0A1V6PGE7_PENDC NVQAKHGV—ISLTCLEERLQR ADPVVLAFDIETTKLPLKFPDAVIDQIMMISYMIDGQG 315 tr|A0A0G4NU82|A0A0G4NU82_PENCA NVEADHGV--ISLTCLEDRLQ RADPVVLAFDIECTKLPLKFPDATMDQIMMISYMIDGQG 315 tr|A0A1V6TET5|A0A1V6TET5_9EURO NVEADHGV—ISLTCLEDRLQR ADPVVLAFDIECTKLPLKFPDAAMDQIMMISYMIDGQG 315 tr|A0A167WJ50|A0A167WJ50_PENCH NVEADHGV—ISLTCLEDRLQR ADPVVLAFDIECTKLPLKFPDAATDQIMMISYMIDGQG 315 tr|A0A3A2Z9P9|A0A3A2Z9P9_9EURO TVEAKHGL—ISLTCLEDRLQR ADPVVLAFDIETTKLPLKFPDSVIDQIIMISYMIDGQG 315 tr|A0A401L0U3|A0A401L0U3_ASPAW TVEAKHGV—ISLTCIEERLTR ADPVVLAFDIETTKLPLKFPDSVIDQIMMISYMIDGQG 315 tr|A0A317VH96|A0A317VH96_ASPEC TVEAKHGV—ISLTCIEERLTR ADPVVLAFDIETTKLPLKFPDSVIDQIMMISYMIDGQG 311 tr|A0A117E1M7|A0A117E1M7_ASPNG TVEAKHGV—ISLTCIEERLAR ADPVVLAFDIETTKLPLKFPDSVIDQIMMISYMIDGQG 315 tr|A0A1D8PTD0|A0A1D8PTD0_CANAL DVYAKHSK—VDFVEDKEKIAF ADPVVLAFDIETTKAPLKFPDAKIDQIMMISYMIDGEG 321 tr|C5M3R9|C5M3R9_CANTT DVYAKHSK—IEIVEDKEKIAF ADPVVLAFDIETTKAPLKFPDAKVDQIMMISYMIDGEG 316 sp|Q6FNY7|DPOE_CANGA KVTH------EGFFVWPDKVAF ADPVVLAFDIETTKAPLKFPEASVDQVMMISYMIDGDG 319 sp|P21951|DPOE_YEAST KVTQ------QGFIEDTRKIAF ADPVVMAFDIETTKPPLKFPDSAVDQIMMISYMIDGEG 259 X-ray tr|A0A7H9HLU1|A0A7H9HLU1_9SACH KVAS------DGLHEITEKVAF ADPVVLAFDIETTKAPLKFPDSAVDQIMMISYMIDGDG 309 sp|Q6CUS7|DPOE_KLULA AVSA------QGLVELEEKVTF ADPVVLAFDIETTKAPLKFPDSAIDQIMMISYMIDGEG 236 sp|Q752B8|DPOE_ASHGO RVTS------GGLVEYTDKVAF ADPVVLAFDIETTKAPLKFPDSAIDQIMMISYMIDGEG 233 : . : : *:*** :* ****:: * tr|A0A3P5YCF9|A0A3P5YCF9_BRACM YNGDFFDWPFIERRASHHEIKMNEELGFRCDHNQGECRAKFVCHLDCFAWVKRDSYLPQG 393 sp|F4HW04|DPOE1_ARATH YNGDFFDWPFIERRASHHGIKMNEELGFRCDQNQGECRAKFACHLDCFAWVKRDSYLPQG 393 tr|D7KI06|D7KI06_ARALL YNGDFFDWPFIERRASHHGIKMNEELGFRCDHNQGECRAKFVCHLDCFAWVKRDSYLPQG 396 tr|A0A8I6WFC8|A0A8I6WFC8_HORVV YNGDFFDWPFLEKRAAHHGIKMNEEIGFQCDSNQGECRAKFSCHLDCFAWVKRDSYLPQG 417 tr|A0A452YAR6|A0A452YAR6_AEGTS YNGDFFDWPFLEKRAAHHGIKMNEEIGFQCDSNQGECRAKFSCHLDCFAWVKRDSYLPQG 357 tr|A0A3B5XXC3|A0A3B5XXC3_WHEAT YNGDFFDWPFLEKRAAHHGIKMNEEIGFQCDSNQGECRAKFSCHLDCFAWVKRDSYLPQG 412 tr|A0A1D6H8Z0|A0A1D6H8Z0_MAIZE YNGDFFDWPFLEKRAAHHGIKMNEEIGFQCDNNQGECRAKFSCHLDCFAWVKRDSYLPQG 390 tr|A0A835B357|A0A835B357_9POAL YNGDFFDWPFLEKRAAHHGVKMNEEIGFQCDNNQGECRAKFSCHLDCFAWVKRDSYLPQG 403 tr|A0A2S3GPC0|A0A2S3GPC0_9POAL YNGDFFDWPFLEKRAAHHGIKMNEEIGFQCDNNQGECRAKFSCHLDCFAWVKRDSYLPQG 408 tr|A0A368PL62|A0A368PL62_SETIT YNGDFFDWPFLEKRAAHHGIKMNEEIGFQCDSNQGECRAKFSCHLDCFAWVKRDSYLPQG 412 tr|A0A7I8KHB8|A0A7I8KHB8_SPIIN YNGDFFDWPFLEKRAAHHGIKMSEELGFVCDSVQGECRAKFSCHLDCFAWVKRDSYLPQG 394 tr|A0A6I9QIG1|A0A6I9QIG1_ELAGV YNGDFFDWPFIEKRAAHHGLKLSEEIGFHCDNNQGECRAKFSCHLDCFAWVKRDSYLPQG 392 tr|A0A8B8JBC2|A0A8B8JBC2_PHODC YNGDFFDWPFLEKRAAHYGLKMSEEIGFQCDNNQGECRAKFSCHLDCFAWVKRDSYLPQG 392 tr|A0A9Q0FIP5|A0A9Q0FIP5_9ROSI YNGDFFDWPFLEARAAHHGFRMSDEVGFSCDKNQGECRAKFACHLDCFAWVKRDSYLPQG 409 tr|A0A2C9VYJ6|A0A2C9VYJ6_MANES YNGDYFDWPFLESRAAYHGLKMSDEVGFSCDKNQGECRAKFACHLDCFAWVKRDSYLPQG 395 tr|A0A7J7C034|A0A7J7C034_TRIWF YNGDFFDWPFLERRAAHHGYILSNELGFQCDKNQGECRAKFACHLDCFAWVKRDSYLPQG 394 tr|A0A2N9F976|A0A2N9F976_FAGSY YNGDFFDWPFLESRAAYHGLKMSEEVGFQCDKNQGECRAKFACHLDCFAWVKRDSYLPQG 413 tr|A0A7N2KME6|A0A7N2KME6_QUELO YNGDFFDWPFLESRAAYHGLKMSEEVGFQCDKNQGECRAKFACHLDCFAWVKRDSYLPQG 393 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE YNGDFFDWPFVETRAAHHGLNMYREIGFQKD-NQGEYRASQAIHMDAFRWVKRDSYLPVG 421 tr|A0A8C7Q9V4|A0A8C7Q9V4_ONCMY YNGDFFDWPFVETRAAHHGLNMYREIGFQKD-NQGEYRASQAIHMDAFRWVKRDSYLPVG 379 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE YNGDFFDWPFIETRASLHGLSMHREIGFQKD-SQGEYKSSQAIHMDCLRWVKRDSYLPVG 421 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC YNGDFFDWPFVETRAALHGLDMYKEIGFQKD-NQGEYKSTQAIHMDCYRWVKRDSYLPMG 419 tr|A0A8J0QX44|A0A8J0QX44_XENTR YNGDFFDWPFVETRASVHGMSMLQEIGFQKD-NQGEYKSPPCIHMDCLRWVKRDSYLPVG 418 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR YNGDFFDWPFVEARAAVHGLSMHQEIGFQKD-SQGEYKSSQCIHMDCLRWVKRDSYLPVG 405 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF YNGDFFDWPFVEARAAVHGLSMYQEIGFQKD-SQGEYKASQCIHMDCLRWVKRDSYLPVG 455 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE YNGDFFDWPFVEARAAAHGLSMYEEIGFQKD-SQGEYKAPQCIHMDCLRWVKRDSYLPVG 393 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA YNGDFFDWPFVEARAAAHGLSMYQEIGFQKD-SQGEYKAPQCIHMDCLRWVKRDSYLPVG 412 tr|A0A2K6CI58|A0A2K6CI58_MACNE YNGDFFDWPFVEARAAVHGLSMQQEIGFQKD-SQGEYKAPQCIHMDCLRWVKRDSYLPVG 420 tr|F6QAQ1|F6QAQ1_MACMU YNGDFFDWPFVEARAAVHGLSMQQEIGFQKD-SQGEYKAPQCIHMDCLRWVKRDSYLPVG 422 tr|G3RQP2|G3RQP2_GORGO YNGDFFDWPFVEARAAVHGLSMQQEIGFQKD-SQGEYKAPQCIHMDCLRWVKRDSYLPVG 399 sp|Q07864|DPOE1_HUMAN YNGDFFDWPFVEARAAVHGLSMQQEIGFQKD-SQGEYKAPQCIHMDCLRWVKRDSYLPVG 420 tr|A0A2I3S482|A0A2I3S482_PANTR YNGDFFDWPFVEARAAVHGLSMQQEIGFQKD-SQGEYKAPQCIHMDCLRWVKRDSYLPVG 420 tr|F6Z911|F6Z911_HORSE YNGDFFDWPFVEARAAVHGLSMYQEIGFQKD-NQGEYKAPQCIHMDCLRWVKRDSYLPVG 420 tr|A0A5G2RB90|A0A5G2RB90_PIG YNGDFFDWPFVEARAAVHGLSMYQEIGFQKD-SQGEYKSSQCIHMDCLRWVKRDSYLPVG 393 tr|A0A420PL83|A0A420PL83_FUSOX YNGDFFDWPFVEARASINGIDMYQEIGWKKD-NEDQYKCNYSVHMDCFHWVNRDSYLPQG 396 tr|A0A1V6PGE7|A0A1V6PGE7_PENDC YNGDFFDWPFVETRASVQGIDMYREIGFRKN-SEDIYQSDYCVHMDCFAWVNRDSYLPQG 431 tr|A0A0G4NU82|A0A0G4NU82_PENCA YNGDFFDWPFVETRASILGLDMYMEIGFRKN-SEDIYQADNCVHMDCFAWVNRDSYLPQG 431 tr|A0A1V6TET5|A0A1V6TET5_9EURO YNGDFFDWPFVETRASILGLDMYMEIGFRKN-SEDIYQADNCVHMDCFAWVNRDSYLPQG 431 tr|A0A167WJ50|A0A167WJ50_PENCH YNGDFFDWPFVETRASILGLDMYMEIGFRKN-SEDIYQADNCVHMDCFAWVNRDSYLPQG 431 tr|A0A3A2Z9P9|A0A3A2Z9P9_9EURO YNGDFFDWPFVEARASVLGIDMYTEIGFRKN-SEDIYQSDHCVHMDCFAWVNRDSYLPQG 431 tr|A0A401L0U3|A0A401L0U3_ASPAW YNGDFFDWPFVEARASVLGIDMYREIGFRKN-SEDIYQSDHCVHMDCFAWVNRDSYLPQG 431 tr|A0A317VH96|A0A317VH96_ASPEC YNGDFFDWPFVEARASVLGIDMYREIGFRKN-SEDIYQSDHCVHMDCFAWVNRDSYLPQG 427 tr|A0A117E1M7|A0A117E1M7_ASPNG YNGDFFDWPFVEARASVLGIDMYREIGFRKN-SEDIYQSDHCVHMDCFAWVNRDSYLPQG 431 tr|A0A1D8PTD0|A0A1D8PTD0_CANAL FNGDFFDWPFVENRTRFHDMDMFEEIGFAKD-NEGEYKSKYCVHMDCFRWVKRDSYLPQG 437 tr|C5M3R9|C5M3R9_CANTT FNGDFFDWPFVEKRARFHDMDMFDEIGFAKD-NEDEYKSKYCVHMDCFRWVKRDSYLPQG 432 sp|Q6FNY7|DPOE_CANGA FNGDFFDWPFIEKRASVRGLDMFEEIGFAPD-SEGEYKSSYCVHMDCFRWVKRDSYLPQG 435 sp|P21951|DPOE_YEAST FNGDFFDWPFIHNRSKIHGLDMFDEIGFAPD-AEGEYKSSYCSHMDCFRWVKRDSYLPQG 375 X-ray tr|A0A7H9HLU1|A0A7H9HLU1_9SACH FNGDFFDWPFIDNRSKIHGLDMFEEIGFLPD-SEGEYKSSYCSHMDCFRWVKRDSYLPQG 425 sp|Q6CUS7|DPOE_KLULA FNGDFFDWPFVENRAKFHGLNMFDEIGFAPD-SEGEYKSSYCTHMDCFRWVKRDSYLPQG 352 sp|Q752B8|DPOE_ASHGO FNGDFFDWPFVEARSKIRGLSMFDEIGFAPD-SEGEYKSSYCAHMDCYRWVKRDSYLPQG 349 :***:*****:. *: : *:*: : :. :. *:*. **:****** * World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 567 tr|A0A3P5YCF9|A0A3P5YCF9_BRACM SHGLKAVTKAKLGYDPLEVNPEDMVQFAMEKPQTMASYSVSDAVATYYLYMTYVHPFIFS 453 sp|F4HW04|DPOE1_ARATH SHGLKAVTKAKLGYDPLEVNPEDMVRFAMEKPQTMASYSVSDAVATYYLYMTYVNPFIFS 453 tr|D7KI06|D7KI06_ARALL SHGLKAVTKAKLGYDPLEVNPEDMVRFAMEKPQTMASYSVSDAVATYYLYMTYVNPFIFS 456 tr|A0A8I6WFC8|A0A8I6WFC8_HORVV SQGLKAVTKAKLGYDPLEVNPEDMVRFAMEQPQTMASYSVSDAVATYYLYMTYVHPFIFS 477 tr|A0A452YAR6|A0A452YAR6_AEGTS SQGLKAVTKAKLGYDPLEVNPEDMVRFAMEQPQTMASYSVSDAVATYYLYMTYVHPFIFS 417 tr|A0A3B5XXC3|A0A3B5XXC3_WHEAT SQGLKAVTKAKLGYDPLEVNPEDMVRFAMEQPQTMASYSVSDAVATYYLYMTYVHPFIFS 472 tr|A0A1D6H8Z0|A0A1D6H8Z0_MAIZE SQGLKAVTKAKLGYDPLEVNPEDMVRFAMEQPQTMASYSVSDAVATYYLYMTYVHPFIFS 450 tr|A0A835B357|A0A835B357_9POAL SQGLKAVTKAKLGYDPLEVNPEDMVRFAMEQPQTMASYSVSDAVATYYLYMTYVHPFIFS 463 tr|A0A2S3GPC0|A0A2S3GPC0_9POAL SQGLKAVTKAKLGYDPLEVNPEDMVRFAMEQPQTMASYSVSDAVATYYLYMTYVHPFIFS 468 tr|A0A368PL62|A0A368PL62_SETIT SQGLKAVTKAKLGYDPLEVNPEDMVRFAMEQPQTMASYSVSDAVATYYLYMTYVHPFIFS 472 tr|A0A7I8KHB8|A0A7I8KHB8_SPIIN SHGLKAVTKAKLGYDPIEVNPEDMVRFAMEEPQTMASYSVSDAVATYYLYMTYVHPFIFS 454 tr|A0A6I9QIG1|A0A6I9QIG1_ELAGV SHGLKAVTKAKLGYDPLEVNPEDMVRFAMEQPQMMASYSVSDAVATYYLYMTYIHPFIFS 452 tr|A0A8B8JBC2|A0A8B8JBC2_PHODC SHGLKAVTKAKLGYDPLEVNPEDMVRFAMEQPQMMASYSVSDAVATYYLYMTYIHPFIFS 452 tr|A0A9Q0FIP5|A0A9Q0FIP5_9ROSI SHGLKAVTKAKLGYDPVEVNPEDMVRFAKDKPQMMASYSVSDAVATYYLYMTYVHPFIFS 469 tr|A0A2C9VYJ6|A0A2C9VYJ6_MANES SQGLKAVTKAKLGYDPLEVNPEDMVRFAKENPQMMASYSVSDAVATYFLYMTYVHPFIFS 455 tr|A0A7J7C034|A0A7J7C034_TRIWF SQGLKAVTKAKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAVSTYYLYMTYVHPFIFS 454 tr|A0A2N9F976|A0A2N9F976_FAGSY SQGLKAVTKAKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFS 473 tr|A0A7N2KME6|A0A7N2KME6_QUELO SQGLKAVTKAKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAVSTYYLYMTYVHPFIFS 453 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE SHNLKAAAKAKLGYDPVELDPEEMCRMATEESQTLATYSVSDAVATYYLYMKYVHPFIFA 481 tr|A0A8C7Q9V4|A0A8C7Q9V4_ONCMY SHNLKAAAKAKLGYDPVELDPEEMCRMATEESQTLATYSVSDAVATYYLYMKYVHPFIFA 439 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE SHNLKAAAKAKLGYDPVELDPEEMCRMATEESQTLATYSVSDAVATYYLYMKYVHPFIFA 481 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFA 479 tr|A0A8J0QX44|A0A8J0QX44_XENTR SHNLKAAAKAKLGYDPVELDPEEMCRMATEEPQVLATYSVSDAVATYYMYMKYVHPFIFA 478 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR SHNLKAAAKAKLGYDPVELDPEDMCRMATEEPQTLATYSVSDAVATYYMYMKYVHPFIFA 465 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFA 515 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFA 453 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFA 472 tr|A0A2K6CI58|A0A2K6CI58_MACNE SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFA 480 tr|F6QAQ1|F6QAQ1_MACMU SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFA 482 tr|G3RQP2|G3RQP2_GORGO SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFA 459 sp|Q07864|DPOE1_HUMAN SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFA 480 tr|A0A2I3S482|A0A2I3S482_PANTR SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFA 480 tr|F6Z911|F6Z911_HORSE SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFA 480 tr|A0A5G2RB90|A0A5G2RB90_PIG SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFA 453 tr|A0A420PL83|A0A420PL83_FUSOX SRGLKAVTVAKLGYDPDELDPELMTPYATERPQTLAEYSVSDAVATYYLYMKYVHPFIFS 456 tr|A0A1V6PGE7|A0A1V6PGE7_PENDC SRGLKAVTVAKLGYDPDELDPELMTPYASERPQTLAEYSVSDAVATYYLYMKYVHPFIFS 491 tr|A0A0G4NU82|A0A0G4NU82_PENCA SRGLKAVTVAKLGYDPDELDPELMTPYASERPQTLAEYSVSDAVATYYLYMKYVHPFIFS 491 tr|A0A1V6TET5|A0A1V6TET5_9EURO SRGLKAVTVAKLGYDPDELDPELMTPYASERPQTLAEYSVSDAVATYYLYMKYVHPFIFS 491 tr|A0A167WJ50|A0A167WJ50_PENCH SRGLKAVTVAKLGYDPDELDPELMTPYASERPQTLAEYSVSDAVATYYLYMKYVHPFIFS 491 tr|A0A3A2Z9P9|A0A3A2Z9P9_9EURO SRGLKAVTVAKLGYDPDELDPELMTPYASERPQTLAEYSVSDAVATYYLYMKYVHPFIFS 491 tr|A0A401L0U3|A0A401L0U3_ASPAW SRGLKAVTVAKLGYDPDELDPELMTPYASERPQTLAEYSVSDAVATYYLYMKYVHPFIFS 491 tr|A0A317VH96|A0A317VH96_ASPEC SRGLKAVTVAKLGYDPDELDPELMTPYASERPQTLAEYSVSDAVATYYLYMKYIHPFIFS 487 tr|A0A117E1M7|A0A117E1M7_ASPNG SRGLKAVTVAKLGYDPDELDPELMTPYASERPQTLAEYSVSDAVATYYLYMKYVHPFIFS 491 tr|A0A1D8PTD0|A0A1D8PTD0_CANAL SQGLKAVTTAKLGYNPTELDPELMTPYAYDKPQLLSEYSVSDAVATYYLYYKYVHPFIFS 497 tr|C5M3R9|C5M3R9_CANTT SQGLKAVTTVKLGYNPTELDPELMTPYAYEKPQLLSEYSVSDAVATYYLYYKYVHPFIFS 492 sp|Q6FNY7|DPOE_CANGA SQGLKAVTQAKLGYNPIELDPELMTPYAYERPQQLSEYSVSDAVATYYLYMKYVHPFIFS 495 sp|P21951|DPOE_YEAST SQGLKAVTQSKLGYNPIELDPELMTPYAFEKPQHLSEYSVSDAVATYYLYMKYVHPFIFS 435 tr|A0A7H9HLU1|A0A7H9HLU1_9SACH SQGLKAVTQVKLGYNPIELDPELMTPYAFEKPQQLSEYSVSDAVATYYLYMKYVHPFIFS 485 sp|Q6CUS7|DPOE_KLULA SQGLKAVTQAKLGYNPLELDPELMTPYAYEKPQILSEYSVSDAVATYYLYMKYVHPFIFS 412 sp|Q752B8|DPOE_ASHGO SQGLKAVTQVKLGYNPIELDPELMTPYAYEKPQHLSEYSVSDAVATYYLYMKYVHPFIFS 409 *:.***.: ****:* *::** * * :. * :: *******:**::* .*::****: PR exo Pol tr|A0A3P5YCF9|A0A3P5YCF9_BRACM LATIIPMVPDEVLRKGSGTLCEMLLMVE---------AYKA NVVCPNKNQADPEKF-YQS 503 sp|F4HW04|DPOE1_ARATH LATIIPMVPDEVLRKGSGTLCEMLLMVE---------AYKA NVVCPNKNQADPEKF-YQN 503 tr|D7KI06|D7KI06_ARALL LATIIPMVPDEVLRKGSGTLCEMLLMVQVSTYLPQAQAYKV NVVCPNKNQADPEKF-YQN 515 tr|A0A8I6WFC8|A0A8I6WFC8_HORVV LATIIPMSPDEVLRKGSGTLCEMLLMVQ---------AFKA SIICPNKHQSDLEKF-YNN 527 tr|A0A452YAR6|A0A452YAR6_AEGTS LATIIPMSPDEVLRKGSGTLCEMLLMVQ---------AFQA NIICPNKHQADLEKF-YNN 467 tr|A0A3B5XXC3|A0A3B5XXC3_WHEAT LATIIPMSPDEVLRKGSGTLCEMLLMVQ---------AFQA NIICPNKHQADLEKF-YNN 522 tr|A0A1D6H8Z0|A0A1D6H8Z0_MAIZE LATIIPMSPDEVLRKGSGTLCEMLLMVQ---------AFKA SVICPNKHQADLEKF-YNN 500 tr|A0A835B357|A0A835B357_9POAL LATIIPMSPDEVLRKGSGTLCEMLLMVQ---------AFKA NVICPNKHQADLEKF-YNN 513 tr|A0A2S3GPC0|A0A2S3GPC0_9POAL LATIIPMSPDEVLRKGSGTLCEMLLMVQ---------AFKA NVICPNKHQADLEKF-YNN 518 tr|A0A368PL62|A0A368PL62_SETIT LATIIPMSPDEVLRKGSGTLCEMLLMVQ---------AFKA NVICPNKHQADLEKF-YNN 522 tr|A0A7I8KHB8|A0A7I8KHB8_SPIIN LATIIPMPPDEVLRKGSGTLCEMLLMVQ---------AYKA NVICPNKHHADPEKF-YNN 504 tr|A0A6I9QIG1|A0A6I9QIG1_ELAGV LATIIPMTPDEVLRKGSGTLCEMLLMVQ---------AYKA NIICPNKHQDDPEKF-YNN 502 tr|A0A8B8JBC2|A0A8B8JBC2_PHODC LATIIPMTPDEVLRKGSGTLCEMLLMVQ---------AYKA NIICPNKHQDDPEKF-YNN 502 tr|A0A9Q0FIP5|A0A9Q0FIP5_9ROSI LSTIIPMSPDEVLRKGSGTLCEMLLMVQ---------AYKA NVICPNKHQSDPEKF-HRN 519 tr|A0A2C9VYJ6|A0A2C9VYJ6_MANES LATIIPMSPDEVLRKGSGTLCEMLLMVQ---------SYKA NVICPNKHQSDPEKF-YKN 505 tr|A0A7J7C034|A0A7J7C034_TRIWF LATIIPMSPDEVLRKGSGTLCEMLLMVQ---------AYKA NVICPNKHQSDPEKF-YKN 504 tr|A0A2N9F976|A0A2N9F976_FAGSY LATIIPMSPDEVLRKGSGTLCEMLLMVQ---------AYEA NVICPNKHQSDPEKF-YTN 523 tr|A0A7N2KME6|A0A7N2KME6_QUELO LATIIPMSPDEVLRKGSGTLCEMLLMVQ---------AYQA NVICPNKHQSDPEKF-YNS 503 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AYHA NIVFPNKQEQVFNKLTDDG 532 tr|A0A8C7Q9V4|A0A8C7Q9V4_ONCMY LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AYHA NIVFPNKQEQVFNKLTDDG 490 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AFHA NIIFPNKQEQVFNKLTDDG 532 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AFHA NIVFPNKQDQVFNKMTGDG 530 tr|A0A8J0QX44|A0A8J0QX44_XENTR LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AYHA NIIFPNKQEAVFNKLTSDG 529 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AFHA NIVFPNKQEQEFNKLTEDG 516 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AFHA NIIFPNKQEQEFNKLTGDG 566 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AFHA NIIFPNKQEQEFNKMTSDG 504 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AFHA NIVFPNKQEQEFNKLTDDG 523 tr|A0A2K6CI58|A0A2K6CI58_MACNE LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AFHA NIIFPNKQEQEFNKLTDDG 531 tr|F6QAQ1|F6QAQ1_MACMU LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AFHA NIIFPNKQEQEFNKLTDDG 533 tr|G3RQP2|G3RQP2_GORGO LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AFHA NIIFPNKQEQEFNKLTGNG 510 sp|Q07864|DPOE1_HUMAN LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AFHA NIIFPNKQEQEFNKLTDDG 531 tr|A0A2I3S482|A0A2I3S482_PANTR LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AFHA NIIFPNKQEQEFNKLTDDG 531 tr|F6Z911|F6Z911_HORSE LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AFHA NIIFPNKQEQEFNKLTDDG 531 tr|A0A5G2RB90|A0A5G2RB90_PIG LCTIIPMEPDEVLRKGSGTLCEALLMVQ---------AFHA NIIFPNKQEQEFNKLTDDG 504 tr|A0A420PL83|A0A420PL83_FUSOX LCTILPLSGDEVLRKGTGTLCEMLLMVQ---------AYQR EIVLPNKYITPKEAF-WDG 506 tr|A0A1V6PGE7|A0A1V6PGE7_PENDC LCTIIPLNADETLRKGTGTLCEMLLMVQ---------AYQH EIILPNKHKDPPEAF-YDG 541 tr|A0A0G4NU82|A0A0G4NU82_PENCA LCTIIPLNADETLRKGTGTLCEMLLMVQ---------AYQH EIVLPNKHKNPPEAF-YEG 541 tr|A0A1V6TET5|A0A1V6TET5_9EURO LCTIIPLNPDETLRKGTGTLCEMLLMVQ---------AYQH EIVLPNKHKNPPEAF-YEG 541 tr|A0A167WJ50|A0A167WJ50_PENCH LCTIIPLNPDETLRKGTGTLCEMLLMVQ---------AYQH EIVLPNKHKNPPEAF-YEG 541 tr|A0A3A2Z9P9|A0A3A2Z9P9_9EURO LCTIVPLNPDDTLRKGTGTLCEMLLMVQ---------AYKG EIILPNKHKDPPESF-YEG 541 tr|A0A401L0U3|A0A401L0U3_ASPAW LCTILPINPDDTLRKGTGTLCEMLLMVQ---------AYQG NIVLPNKHKDPPESF-YEG 541 tr|A0A317VH96|A0A317VH96_ASPEC LCTILPINPDDTLRKGTGTLCEMLLMVQ---------AYQG NIVLPNKHKDPPESF-YEG 537 tr|A0A117E1M7|A0A117E1M7_ASPNG LCTILPINPDDTLRKGTGTLCEMLLMVQ---------AYQG NIVLPNKHKDPPESF-YEG 541 tr|A0A1D8PTD0|A0A1D8PTD0_CANAL LCTIIPLNPDEVLRKGTGTLCEMLLSVQ---------AYEG NILLPNKHSDPIERF-YEG 547 tr|C5M3R9|C5M3R9_CANTT LCTIIPLNPDEVLRKGTGTLCEMLLSVQ---------AYEG NILLPNKHADPIERF-YDG 542 sp|Q6FNY7|DPOE_CANGA LCTIIPLNPDEVLRKGTGTLCEMLLMVQ---------AYQG NILLPNKHTDPLERF-YDG 545 sp|P21951|DPOE_YEAST LCTIIPLNPDETLRKGTGTLCEMLLMVQ---------AYQH NILLPNKHTDPIERF-YDG 485 tr|A0A7H9HLU1|A0A7H9HLU1_9SACH LCTIIPLNPDEVLRKGTGTLCEMLLMVQ---------AYNG NILLPNKYTDPLERF-YDG 535 sp|Q6CUS7|DPOE_KLULA LCTIIPLNPDEVLRKGTGTLCEMLLMVQ---------AYQN SVLLPNKHTDPIERF-YDG 462 sp|Q752B8|DPOE_ASHGO LCTVLPLNPDEVLRKGTGTLCEMLLMVQ---------AYDH GILLPNKHTEPIERF-YDG 459 *.*::*: *:.****:***** ** *: ::. :: *** : : . World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 568 ZBM tr|A0A3P5YCF9|A0A3P5YCF9_BRACM EKLRDCPIREEGPLIYHLDVAAMYPNIILTNRL-----QPPSIVTNEICTACDFNRPGKT 678 sp|F4HW04|DPOE1_ARATH EKLRDDPIREEGPLIYHLDVAAMYPNIILTNRL-----QPPSIVTDEICTACDFNRPGKT 634 tr|D7KI06|D7KI06_ARALL EKLRDDPIREEGPLIYHLDVAAMYPNIILTNRLQVHLISPPSIVTDEVCTACDFNLPGKT 633 tr|A0A8I6WFC8|A0A8I6WFC8_HORVV VSLRDHPTREECPLIYHLDVAAMYPNIILTNRL-----QPPSIVTDVDCTACDFNRPGKN 658 tr|A0A452YAR6|A0A452YAR6_AEGTS VSLRDHPTREECPLIYHLDVAAMYPNIILTNRL-----QPPSIVTDVDCTACDFNRPGKN 598 tr|A0A3B5XXC3|A0A3B5XXC3_WHEAT VSLRDHPTREECPLIYHLDVAAMYPNIILTNRL-----QPPSIVTDVDCTACDFNRPGKN 653 tr|A0A1D6H8Z0|A0A1D6H8Z0_MAIZE VALRDHPIREERPLIYHLDVAAMYPNIILTNRL-----QPPSIVTDVDCTACDFNRPGKN 631 tr|A0A835B357|A0A835B357_9POAL ISLRDHPIREERPLIYHLDVAAMYPNIILTNRL-----QPPSIVTDVDCTACDFNRPGKN 644 tr|A0A2S3GPC0|A0A2S3GPC0_9POAL VSLRDHPIREERPLIYHLDVAAMYPNIILTNRL-----QPPSIVTDVDCTACDFNRPGKN 649 tr|A0A368PL62|A0A368PL62_SETIT VSLRDHPIREERPLIYHLDVAAMYPNIILTNRL-----QPPSIVTDVDCTACDFNRPGKN 653 tr|A0A7I8KHB8|A0A7I8KHB8_SPIIN QSLRDHPIRDECPLIYHLDVAAMYPNIILTNRL-----QPPSIVSDEVCTACDFNRPGKT 635 tr|A0A6I9QIG1|A0A6I9QIG1_ELAGV IFLRDHPMREECPLIYHLDVAAMYPNIILTNRL-----QPPSIVSDVVCTACDFNRPGKN 633 tr|A0A8B8JBC2|A0A8B8JBC2_PHODC ILLRDRPTREECPLIYHLDVAAMYPNIILTNRL-----QPLSIVSDVVCTACDFNRPGKN 633 tr|A0A9Q0FIP5|A0A9Q0FIP5_9ROSI LSLRDDPVREECPLIYHLDVAAMYPNIILTNRL-----QPPSIVTDEICTACDFNRPDKT 650 tr|A0A2C9VYJ6|A0A2C9VYJ6_MANES VRLRDEPIREECPLIYHLDVAAMYPNIILTNRL-----QPPSIVTEEICTACDFNRPDKT 636 tr|A0A7J7C034|A0A7J7C034_TRIWF VRLQDEPTREECPLIYHLDVAAMYPNIILTNRL-----QPPSIVTDDVCTACDFNRPGKT 635 tr|A0A2N9F976|A0A2N9F976_FAGSY --------------------------------------KPPSIVTDEVCTACDFNRPGKT 619 tr|A0A7N2KME6|A0A7N2KME6_QUELO VRLQGEPIRDECPLIYHLDVAAMYPNIILTNRL-----QPPSIVTDEVCTACDFNRPGKT 634 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE ISLKEVPNRIECPLIYHLDVAAMYPNIILTNRL-----QPSAMVDEATCAACDFNKPGAN 663 tr|A0A8C7Q9V4|A0A8C7Q9V4_ONCMY LSLKEVPNRIECPLIYHLDVAAMYPNIILTNRL-----QPSAMVDEATCAACDFNKPGAN 621 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE ISLKEVPNRIECPLIYHLDVGAMYPNIILTNRL-----QPSAMVDEATCAACDFNKPGAT 663 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC ISLKEVPNRIECPLIYHLDVGAMYPNIILTNRL-----QPSAVVDEATCAACDFNKHGAT 661 tr|A0A8J0QX44|A0A8J0QX44_XENTR NSLKEVPNRIECPLIYHLDVGAMYPNIILTNRL-----QPSAMVDEVTCAACDFNKPGAT 660 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR TSLKDVPNRIECPLIYHLDVGAMYPNIILTNRL-----QPSAIVNEATCAACDFNKPGAN 647 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF TSLKDVPNRIECPLIYHLDVGAMYPNIILTNRL-----QPSAVVDEATCAACDFNKPGAN 697 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE TSLKDVPNRIECPLIYHLDVGAMYPNIILTNRL-----QPSAMVDEATCAACDFNKPGAN 635 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA TSLRDVPNRIECPLIYHLDVGAMYPNIILTNRL-----QPSAMVDEATCAACDFNKPGAN 654 tr|A0A2K6CI58|A0A2K6CI58_MACNE ASLKDVPSRIECPLIYHLDVGAMYPNIILTNRL-----QPSAMVDEATCAACDFNKPGAN 662 tr|F6QAQ1|F6QAQ1_MACMU ASLKDVPSRIECPLIYHLDVGAMYPNIILTNRL-----QPSAMVDEATCAACDFNKPGAN 664 tr|G3RQP2|G3RQP2_GORGO ASLKDVPSRIECPLIYHLDVGAMYPNIILTNRL-----QPSAMVDEATCAACDFNKPGAN 641 sp|Q07864|DPOE1_HUMAN ASLKDVPSRIECPLIYHLDVGAMYPNIILTNRL-----QPSAMVDEATCAACDFNKPGAN 662 tr|A0A2I3S482|A0A2I3S482_PANTR ASLKDVPSRIECPLIYHLDVGAMYPNIILTNRL-----QPSAMVDEATCAACDFNKPGAN 662 tr|F6Z911|F6Z911_HORSE TSLKDVPNRIECPLIYHLDVGAMYPNIILTNRL-----QPSAMVDEATCAACDFNKPGAN 662 tr|A0A5G2RB90|A0A5G2RB90_PIG TSLKDVPNRIECPLIYHLDVGAMYPNIILTNRL-----QPSAMVDEATCAACDFNKPGAN 635 tr|A0A420PL83|A0A420PL83_FUSOX NKLKETPNRNEKPLIYHLDVASMYPNIMTTNRL-----QPDSMIQESDCAACDFNRPGKT 637 tr|A0A1V6PGE7|A0A1V6PGE7_PENDC LDLKNNPVRNEVPFIYHLDVASMYPNIMITNRL-----QPDSLIKESDCAACDFNRPGKT 672 tr|A0A0G4NU82|A0A0G4NU82_PENCA LDLKNNPVRNEVPFIYHLDVASMYPNIMITNRL-----QPDSLIDESNCAACDFNRPGKT 672 tr|A0A1V6TET5|A0A1V6TET5_9EURO LDLKSNPVRNEVPFIYHLDVASMYPNIMITNRL-----QPDSLIDESNCAACDFNRPGKT 672 tr|A0A167WJ50|A0A167WJ50_PENCH LDLKNNPVRNEVPFIYHLDVASMYPNIMITNRL-----QPDSLIDESNCAACDFNRPGKT 672 tr|A0A3A2Z9P9|A0A3A2Z9P9_9EURO ADMRDRPVRDEVPFIYHLDVASMYPNIMITNRL-----QPDSMIQESNCAACDFNRPGKT 672 tr|A0A401L0U3|A0A401L0U3_ASPAW IDLKERPHRDEVPFIYHLDVASMYPNIMITNRL-----QPDSMIQESNCAACDFNRPGKT 672 tr|A0A317VH96|A0A317VH96_ASPEC IDLKERPHRDEVPFIYHLDVASMYPNIMITNRL-----QPDSMIQESNCAACDFNRPGKS 668 tr|A0A117E1M7|A0A117E1M7_ASPNG IDLKERPHRDEVPFIYHLDVASMYPNIMITNRL-----QPDSMIQESNCAACDFNRPGKS 672 tr|A0A1D8PTD0|A0A1D8PTD0_CANAL LELKNNPSRQEKPLIYHVDVASMYPNIMTSNRL-----QPDSMKSEEDCAACDFNRPGKN 678 tr|C5M3R9|C5M3R9_CANTT LNLKNTPIRHEKPLIYHVDVASMYPNIMTTNRL-----QPDSMKTEEDCAACDFNRPGKN 673 sp|Q6FNY7|DPOE_CANGA LELKENNKRHEKPLIYHVDVASMYPNIMTTNRL-----QPDSIKTEKDCASCDFNRPGKS 676 sp|P21951|DPOE_YEAST LELKENNIRNELPLIYHVDVASMYPNIMTTNRL-----QPDSIKAERDCASCDFNRPGKT 616 tr|A0A7H9HLU1|A0A7H9HLU1_9SACH LDLKTNNKRCELPLIYHVDVASMYPNIMTTNRL-----QPDSMKTERDCASCDFNRPGKS 666 sp|Q6CUS7|DPOE_KLULA TDLKINNKRNELPLIYHVDVASMYPNIMTTNRL-----QPDSMKDEKDCASCDFNRPGKS 593 sp|Q752B8|DPOE_ASHGO QELKMNNKRKELPLIYHVDVASMYPNIMTTNRL-----QPDSMKTERDCASCDFNRPGKK 590 .* :: : *::**** . . tr|A0A3P5YCF9|A0A3P5YCF9_BRACM CLRKLEWVWRGVTYMAKKSDYYHLKKQIESEFVDAGANIQ--SSKSFLDLPKVEQQSKLK 736 sp|F4HW04|DPOE1_ARATH CLRKLEWVWRGVTFMGKKSDYYHLKKQIESEFVDAGANIM--SSKSFLDLPKVDQQSKLK 692 tr|D7KI06|D7KI06_ARALL CLRKLEWVWRGVTFMGKKSDYYHLKKQIESEFVDAGANIQ--SSKSFLDLPKLDQQSKLK 691 tr|A0A8I6WFC8|A0A8I6WFC8_HORVV CLRTLEWVWRGETYTAKKSDYHHIKRQIESEMIQAG-GVT--SSKPFLDLSKPEHLLKLK 715 tr|A0A452YAR6|A0A452YAR6_AEGTS CLRTLEWVWRGETYTAKKSDYHHIKRQIESEMIQTG-GVT--SSKPFLDLSKPEHLLKLK 655 tr|A0A3B5XXC3|A0A3B5XXC3_WHEAT CLRTLEWVWRGETYTAKKSDYHHIKRQIESEMIQTG-GVT--SSKPFLDLSKPEHLLKLK 710 tr|A0A1D6H8Z0|A0A1D6H8Z0_MAIZE CLRKLEWVWRGETYMAKKNDYYHIKRQIESELIQSG-GIA--SSKPFLDLSKPEHLLKLK 688 tr|A0A835B357|A0A835B357_9POAL CLRKLEWVWRGETYMAKKNDYYHIKRQIESELIQSG-GIA--STKPFLDLSKPEHLLKLK 701 tr|A0A2S3GPC0|A0A2S3GPC0_9POAL CLRNLEWVWRGETYMAKKNDYYHIKRQIESELIQSG-GIA--SSKPFLDLSKPEHLLKLK 706 tr|A0A368PL62|A0A368PL62_SETIT CLRKLEWVWRGETYTAKKNDYYHIKRQIESELIQSG-GIA--SSKPFLDLSKPEHLLKLK 710 tr|A0A7I8KHB8|A0A7I8KHB8_SPIIN CLRKLEWVWRGETFTAKKSDYYHLKKQIESELVEVG-EDR--LSKAFLDLPKSEQQLKMK 692 tr|A0A6I9QIG1|A0A6I9QIG1_ELAGV CLRKLEWVWRGEIYMAKKSDYYHIKKQIESELVENG-EGQ--AIKPFLDLPKSEQQLKLK 690 tr|A0A8B8JBC2|A0A8B8JBC2_PHODC CLRKLEWVWRGETYMAKKSDYYHIKKQIESELVENG-EGQ--TIKPFLDLPKSEQQLKLK 690 tr|A0A9Q0FIP5|A0A9Q0FIP5_9ROSI CLRTLEWVWRGETYMAKKSDYYHLKKQIESEFVDGA-DGQ--LSKSFLDMPKVEQQLKLK 707 tr|A0A2C9VYJ6|A0A2C9VYJ6_MANES CLRKLEWVWRGEIFMAKKSDYYHLKKQIESEFVDGT-DGQ--LSKSFLDLSKVDQQSKLK 693 tr|A0A7J7C034|A0A7J7C034_TRIWF CLRKLEWVWRGDIFMAKKSDYYHLKKQIESEFVEGG-NGQ--LSKSFLDLPKTEQQLKLK 692 tr|A0A2N9F976|A0A2N9F976_FAGSY CLRKLEWVWRGEIFMAKKSDYYHLKKQIESEFVDGA-GGK--LPKSFLDLPKTEQQLRLK 676 tr|A0A7N2KME6|A0A7N2KME6_QUELO CLRKLEWVWRGETFMAKKSDYYHLKKQIESEFVDGT-GGQ--LPKSFLDLPKTEQQLRLK 691 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE CQRKMSWQWRGEIMPASRSEFHRIQQQLESEKFPPLFPNG--RPRAFHELNLEEQARHEK 721 tr|A0A8C7Q9V4|A0A8C7Q9V4_ONCMY CQRKMSWQWRGEIMPASRSEFHRIQQQLESEKFPPLFPNG--RPRAFHELNLEEQARHEK 679 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE CQRKMAWQWRGEIMPATRSEFHRIQQQLESEKFPPFFPNG--PPRAFHTLNREEQAKHEK 721 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC CQRKMAWQWRGEIMPASRSEFHRIQQQLESEKFPPYFPNG--PPRAFHSLNREEQAKHEK 719 tr|A0A8J0QX44|A0A8J0QX44_XENTR CQRQMTWQWRGEFMPASRSEYHRIQQQLESEKFPPLFPGK--PPRAFHELTREEQAKYEK 718 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR CQRKMTWQWRGEFMPASRSEYHRIQQQLESEKFPPLFPDK--PARAFHELSREEQAKYEK 705 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF CQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLTAEG--PARAFHELSREEQAKYEK 755 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE CQRKMAWQWRGEFMPASRSEYHRIRHQLESEKFPPLTPEG--PARAFHELSREEQAKYEK 693 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA CQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPALIAEG--PTRAFHELSREEQAKYEK 712 tr|A0A2K6CI58|A0A2K6CI58_MACNE CQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPALFPEG--PARAFHELSREEQAKYEK 720 tr|F6QAQ1|F6QAQ1_MACMU CQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPALFPEG--PARAFHELSREEQAKYEK 722 tr|G3RQP2|G3RQP2_GORGO CQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEG--PARAFHELSREEQAKYEK 699 sp|Q07864|DPOE1_HUMAN CQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEG--PARAFHELSREEQAKYEK 720 tr|A0A2I3S482|A0A2I3S482_PANTR CQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEG--PARAFHELSREEQAKYEK 720 tr|F6Z911|F6Z911_HORSE CQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLTPEG--PARAFHELSREEQAKYEK 720 tr|A0A5G2RB90|A0A5G2RB90_PIG CQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLTPEG--PARAFHELSREEQAKYEK 693 tr|A0A420PL83|A0A420PL83_FUSOX CDRRLPWAWRGEYLPPKRDEYNMIKNALQNEKFPGKYPSS--PMRTFQELSADEQANLVR 695 tr|A0A1V6PGE7|A0A1V6PGE7_PENDC CDRRMPWAWRGEFLPAKRDEYNMIRRATANERFPGKYKNS--PMRSFGDLSVEEQAAVIK 730 tr|A0A0G4NU82|A0A0G4NU82_PENCA CDRRMPWAWRGEFLPAKRDEYNMIRRATANERFPGRYPNS--PMRSFGELSIEEQAGVVK 730 tr|A0A1V6TET5|A0A1V6TET5_9EURO CDRRMPWAWRGEFLPAKRDEYNMIRRATANERFPGRHPKS--PMRSFNELSIEEQAGVVK 730 tr|A0A167WJ50|A0A167WJ50_PENCH CDRRMPWAWRGEFLPAKRDEYNMIRRATANERFPGRHPKS--PMRSFNELSTEEQAAVVK 730 tr|A0A3A2Z9P9|A0A3A2Z9P9_9EURO CDRRLPWAWRGEFLPAKRDEYNMIRQAVANERFPGRTKNS--PMRLFTDMSAEEQASIVK 730 tr|A0A401L0U3|A0A401L0U3_ASPAW CDRRLPWAWRGEFLPAKRDEYNMIRQAVANERFPGKHKNS--PMRSFGDMSIEEQAGIIK 730 tr|A0A317VH96|A0A317VH96_ASPEC CDRRMPWAWRGEFLPAKRDEYNMIRQAVANERFPGKHKNS--PMRSFGDMSIEEQAGIVK 726 tr|A0A117E1M7|A0A117E1M7_ASPNG CDRRMPWAWRGEFLPAKRDEYNMIRQAVANERFPGKHKNS--PMRSFGDMSIEEQAGIVK 730 tr|A0A1D8PTD0|A0A1D8PTD0_CANAL CDRRLPWAWRGEYYPAEMNEYNMIKRTLQNETFPGARPWL--PPRTFDELSYAEQAAKIK 736 tr|C5M3R9|C5M3R9_CANTT CDRRLPWAWRGEYYPAEMNEYNMIKRTLQNETFPGAKPWL--PPRTFDELPYAEQASLIK 731 sp|Q6FNY7|DPOE_CANGA CARRLKWAWRGEFYPAKSDEYNMIKRALQNETFPNKNKFSKKPYLTFDELSYSEQVAHIK 736 sp|P21951|DPOE_YEAST CARKLKWAWRGEFFPSKMDEYNMIKRALQNETFPNKNKFSKKKVLTFDELSYADQVIHIK 676 tr|A0A7H9HLU1|A0A7H9HLU1_9SACH CDRRLKWSWRGEFFPAKMDEYNMVKRALQNETFLNKNKFSKKKYLTFDELSYAEQVSHIK 726 sp|Q6CUS7|DPOE_KLULA CDRRLKWAWRGEFFPAKMDEYGMVKRALQNELFPNKNPKSKKQFLTFEELSYSDQVSHIK 653 sp|Q752B8|DPOE_ASHGO CDRRLKWTWRGEFLPAKMDEYAMVKRALMNEVFPNKYKNTTKKLLTFDELSYSEQVSHIK 650 * * : * *** .:: ::. .* . * : :: : World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 569 ZBM tr|A0A3P5YCF9|A0A3P5YCF9_BRACM ERLKKYCQKAYRRVLDKPSTEILEAGICMRENPFYVDTVRSFRDRRYEYKTLNKVWKGKL 796 sp|F4HW04|DPOE1_ARATH ERLKKYCQKAYKRVLDKPITEVREAGICMRENPFYVDTVRSFRDRRYEYKTLNKVWKGKL 752 tr|D7KI06|D7KI06_ARALL ERLKKYCQKAYKRVLDKPITEVREAGICMRENPFYVDTVRSFRDRRYEYKTLNKVWKGKL 751 tr|A0A8I6WFC8|A0A8I6WFC8_HORVV DRLKKYCQKAYKRVVDKPITEVREAGICMRENSFYVDTVRSFRDRRYEYKGLNKTWKGKL 775 tr|A0A452YAR6|A0A452YAR6_AEGTS DRLKKYCQKAYKRVVDKPITEVREAGICMRENSFYVDTVRSFRDRRYEYKGLNKTWKGKL 715 tr|A0A3B5XXC3|A0A3B5XXC3_WHEAT DRLKKYCQKGYKRVVDKPITEVREAGICMRENSFYVDTVRSFRDRRYEYKGLNKTWKGKL 770 tr|A0A1D6H8Z0|A0A1D6H8Z0_MAIZE DRLKKYCQKAHKRVVDKPITEVREAGICMRENSFYVDTVRSFRDRRYEYKGLNKTWKGKL 748 tr|A0A835B357|A0A835B357_9POAL DRLKKYCQKAHKRVVDKPITEVREAGICMRENSFYVDTVRSFRDRRYEYKGLNKTWKGKL 761 tr|A0A2S3GPC0|A0A2S3GPC0_9POAL DRLKKYCQKAHKRVVDKPITEVREAGICMRENSFYVDTVRSFRDRRYEYKGLNKTWKGKL 766 tr|A0A368PL62|A0A368PL62_SETIT DRLKKYCQKAHKRVVDKPITEVREAGICMRENSFYVDTVRSFRDRRYEYKGLNKTWKGKL 770 tr|A0A7I8KHB8|A0A7I8KHB8_SPIIN ERLKKYCQKAYKRVLDKPLTELREAWICMRENPFYVDTVRSFRDRRYEYKGLNKVWKGKL 752 tr|A0A6I9QIG1|A0A6I9QIG1_ELAGV ERLRKYSQKAYKRVLDKPITELREAGICMRENPFYVDTVRSFRDRRYEYKGLNKVWKGKL 750 tr|A0A8B8JBC2|A0A8B8JBC2_PHODC ERLRKYCQKAYKRVLDKPITELREAGICMRENPFYVDTVRSFRDRRYEYKSLNKLWKGKL 750 tr|A0A9Q0FIP5|A0A9Q0FIP5_9ROSI ERLKKYCQKAYKRVLDKPVTEVREAGICMRENSFYVDTVRSFRDRRYEYKGLNKVWKGKL 767 tr|A0A2C9VYJ6|A0A2C9VYJ6_MANES ERLKKYCQKAYKRVLDKPVTELREAGICMRENPFYVDTVRSFRDRRYEYKGLNKVWKGKL 753 tr|A0A7J7C034|A0A7J7C034_TRIWF ERLKKYCQKAYKRVLDKPVTELREGGICMRENPFYVDTVRSFRDRRYEYKGLNKTWKGKL 752 tr|A0A2N9F976|A0A2N9F976_FAGSY ERLKKYCQKAYKRVIDKPVTELREAGICMRENPFYVDTVRSFRDRRYEYKGLNKVWKGKL 736 tr|A0A7N2KME6|A0A7N2KME6_QUELO ERLKKYSQKAYKRVLDKPVTELREAGICMRENPFYVDTVRSFRDRRYEYKGLNKVWKGKL 751 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE KRLGDYCRRAYKKVRQTK-LEERVTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKL 780 tr|A0A8C7Q9V4|A0A8C7Q9V4_ONCMY KRLGDYCRRAYKKVRQTK-LEERVTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKL 738 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE KRLADYCKRAYKKIHITK-LEERVTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKL 780 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC KRLADYCKRAYKKTHVTK-LEERTTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKL 778 tr|A0A8J0QX44|A0A8J0QX44_XENTR KRLADYCRKAYKKIHVTR-LEEKVTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKL 777 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR KRLADYCRKAYKKIHITK-VEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKL 764 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF RRLSDYCRKAYKKIHVTR-VEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKL 814 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE RRLADYCRKAYKKIHVTK-VEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKL 752 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA RRLADYCRKAYKKIHVTK-VEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKRKL 771 tr|A0A2K6CI58|A0A2K6CI58_MACNE RRLADYCRKAYKKIHITK-VEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKL 779 tr|F6QAQ1|F6QAQ1_MACMU RRLADYCRKAYKKIHITK-VEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKL 781 tr|G3RQP2|G3RQP2_GORGO RRLADYCRKAYKKIHITK-VEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKL 758 sp|Q07864|DPOE1_HUMAN RRLADYCRKAYKKIHITK-VEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKL 779 tr|A0A2I3S482|A0A2I3S482_PANTR RRLADYCRKAYKKIHITK-VEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKL 779 tr|F6Z911|F6Z911_HORSE RRLADYCRKAYKKIHVTK-VEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKL 779 tr|A0A5G2RB90|A0A5G2RB90_PIG RRLADYCRKAYKKIHVTK-VEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKL 752 tr|A0A420PL83|A0A420PL83_FUSOX KRLQLYSQKVYHKIHDST-TIVREAIICQRENPFYINTVRDFRDRRYDYKGKAKVWKGKT 754 tr|A0A1V6PGE7|A0A1V6PGE7_PENDC KRLQDYSKKIYHKIHDSK-TMEREAIICQRENPFYVDTVRSFRDRRYDFKGKQKVWKNKT 789 tr|A0A0G4NU82|A0A0G4NU82_PENCA KRLQDYSKKIYHKIHDSK-TMEREAIICQRENPFYVDTVRDFRDRRYDFKGKQKSWKNKA 789 tr|A0A1V6TET5|A0A1V6TET5_9EURO KRLQDYSKKIYHKIHDSK-TMEREAIICQRENPFYVDTVRAFRDRRYDFKGKQKSWKNKA 789 tr|A0A167WJ50|A0A167WJ50_PENCH KRLQDYSKKIYHKIHDSK-TMEREAIICQRENPFYVDTVRAFRDRRYDFKGKQKSWKNKA 789 tr|A0A3A2Z9P9|A0A3A2Z9P9_9EURO KRLQDYSKKIYHKIHDSK-TMTREAIICQRENPFYVNTVRSFRDRRYDFKGKQKVWKNKT 789 tr|A0A401L0U3|A0A401L0U3_ASPAW KRLQDYSKKIYHKIHDSK-TMVREAIICQRENPFYVDTVRSFRDRRYDFKGKQKVWKGKT 789 tr|A0A317VH96|A0A317VH96_ASPEC KRLQDYSKKIYHKIHDSK-TMVREAIICQRENPFYVDTVRSFRDRRYDFKGKQKVWKGKT 785 tr|A0A117E1M7|A0A117E1M7_ASPNG KRLQDYSKKIYHKIHDSK-TMVREAIICQRENPFYVDTVRSFRDRRYDFKGKQKVWKGKT 789 tr|A0A1D8PTD0|A0A1D8PTD0_CANAL KRISDYSRKVYHRVKSSK-VVTREAIICQRENPFYVDTVRSFRDRRYEFKGLAKVWKGKV 795 tr|C5M3R9|C5M3R9_CANTT KRISDYSRKVYHRVKASK-VVTREAIICQRENPFYVNTVRSFRDRRYEFKGLAKVWKGKV 790 sp|Q6FNY7|DPOE_CANGA KRLTEYSKKVYHRIKVSE-IVEREAIVCQRENPFYVNTVKSFRDRRYEFKGLAKKWKGKM 795 sp|P21951|DPOE_YEAST KRLTEYSRKVYHRVKVSE-IVEREAIVCQRENPFYVDTVKSFRDRRYEFKGLAKTWKGNL 735 tr|A0A7H9HLU1|A0A7H9HLU1_9SACH KRLTDYSRKVYHRVKVSE-VVERESIVCQRENPFYVDTVRSFRDRRYEFKGLAKTWKGKL 785 sp|Q6CUS7|DPOE_KLULA KRLTDYSRKVYHRVKVTE-TVEREAIVCQRENPFYVNTVRSFRDRRYEFKGLAKLWKGKL 712 sp|Q752B8|DPOE_ASHGO KRLSEYSRKVYHRVKVTE-SVVRESIVCQRENPFYVNTVRSFRDRRYEFKGHAKTWKKKL 709 *: *.:: ::: . :* *** **::**: ******::* * ** : tr|A0A3P5YCF9|A0A3P5YCF9_BRACM SEAKASGNS-IKIQEAQDMVVVYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 855 sp|F4HW04|DPOE1_ARATH SEAKASGNS-IKIQEAQDMVVVYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 811 tr|D7KI06|D7KI06_ARALL SEAKASGNS-IKIQEAQDMVVVYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 810 tr|A0A8I6WFC8|A0A8I6WFC8_HORVV SEAKASGNS-IKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 834 tr|A0A452YAR6|A0A452YAR6_AEGTS SEAKASGNS-IKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 774 tr|A0A3B5XXC3|A0A3B5XXC3_WHEAT SEAKASGNS-IKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 829 tr|A0A1D6H8Z0|A0A1D6H8Z0_MAIZE AEAKASGNS-MKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 807 tr|A0A835B357|A0A835B357_9POAL AEAKASGNS-MKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 820 tr|A0A2S3GPC0|A0A2S3GPC0_9POAL AEAKASGNS-MKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 825 tr|A0A368PL62|A0A368PL62_SETIT AEAKASGNS-IKIQEAQDLVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 829 tr|A0A7I8KHB8|A0A7I8KHB8_SPIIN EDAKNSGNS-MKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 811 tr|A0A6I9QIG1|A0A6I9QIG1_ELAGV AEAKSSGNS-IKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 809 tr|A0A8B8JBC2|A0A8B8JBC2_PHODC AEAKSSGNS-MKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 809 tr|A0A9Q0FIP5|A0A9Q0FIP5_9ROSI SEAKASGNS-IKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 826 tr|A0A2C9VYJ6|A0A2C9VYJ6_MANES SEAKASGNS-IKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 812 tr|A0A7J7C034|A0A7J7C034_TRIWF AEAKASGNS-IKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 811 tr|A0A2N9F976|A0A2N9F976_FAGSY SEAKASGNS-IKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 795 tr|A0A7N2KME6|A0A7N2KME6_QUELO SEAKASGNS-IKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYT 810 tr|A0A4W5MAK2|A0A4W5MAK2_9TELE SAAQESGDA-ALVKRCKNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCYT 839 tr|A0A8C7Q9V4|A0A8C7Q9V4_ONCMY SAAQESGDA-ALVKRCKNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCYT 797 tr|A0A6P7J2Q5|A0A6P7J2Q5_9TELE STAQDNGDA-AEVKRCKNMEILYESLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCYT 839 tr|A0A6P8SZE0|A0A6P8SZE0_GYMAC SAAQESGDA-AEVKRCKNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFT 837 tr|A0A8J0QX44|A0A8J0QX44_XENTR SSACESGDA-AEIKRCKNMEILYESLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCYT 836 tr|A0A4X2K1K8|A0A4X2K1K8_VOMUR SAATEMGDA-AEVKRCRNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFT 823 tr|A0A5F4D2S5|A0A5F4D2S5_CANLF SAAMEVGDA-AEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRRGARWYSMEMAGIVCFT 873 tr|A0A7J7RQ84|A0A7J7RQ84_RHIFE CAAVEAGDA-AEVRRCRNMEVLYESLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFT 811 tr|A0A6P6D0R3|A0A6P6D0R3_PTEVA CAAVEAGDA-AEVRRCKNMEVLYESLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFT 830 tr|A0A2K6CI58|A0A2K6CI58_MACNE SAAVEVGDA-AEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFT 838 tr|F6QAQ1|F6QAQ1_MACMU SAAVEVGDA-AEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFT 840 tr|G3RQP2|G3RQP2_GORGO SAAVEVGDA-AEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFT 817 sp|Q07864|DPOE1_HUMAN SAAVEVGDA-AEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFT 838 tr|A0A2I3S482|A0A2I3S482_PANTR SAAVEVGDA-AEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFT 838 tr|F6Z911|F6Z911_HORSE SAAVEVGDA-AEVKRCRNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFT 838 tr|A0A5G2RB90|A0A5G2RB90_PIG SAAVEAGDA-AEVKRCRNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFT 811 tr|A0A420PL83|A0A420PL83_FUSOX SSLQSAGAAQSEVDAAKKMIILYDSLQLAHKVILNSFYGYVMRKGSRWYSLEMAGVTCLT 814 tr|A0A1V6PGE7|A0A1V6PGE7_PENDC DSLQASGASAAEIEEAKKMIVLFDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLT 849 tr|A0A0G4NU82|A0A0G4NU82_PENCA DGLQSSGGSAAEIEEAKKMIILYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLT 849 tr|A0A1V6TET5|A0A1V6TET5_9EURO DSLQSSGGSAAEIDEAKKMIILFDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLT 849 tr|A0A167WJ50|A0A167WJ50_PENCH DGLQSSGGSAAEIDEAKKMIILFDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLT 849 tr|A0A3A2Z9P9|A0A3A2Z9P9_9EURO DSLRSSGASAAEIEEANKMIILFDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLT 849 tr|A0A401L0U3|A0A401L0U3_ASPAW DALKSSGASAAEVEEAKKMIVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLT 849 tr|A0A317VH96|A0A317VH96_ASPEC DALKSSGASAAEVEEAKKMIVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLT 845 tr|A0A117E1M7|A0A117E1M7_ASPNG DALKSSGASAAEVEEAKKMIVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLT 849 tr|A0A1D8PTD0|A0A1D8PTD0_CANAL GKIASNDTI--ARDEAKKMVVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLT 853 tr|C5M3R9|C5M3R9_CANTT GKIDASDTI--ARDEAKKMVVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLT 848 sp|Q6FNY7|DPOE_CANGA SSIDRNDKH--GKDEANKMVVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGITCLT 853 sp|P21951|DPOE_YEAST SKIDPSDKH--ARDEAKKMIVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGITCLT 793 tr|A0A7H9HLU1|A0A7H9HLU1_9SACH SSIPSADKH--ARDEAKKMIVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGITCLT 843 sp|Q6CUS7|DPOE_KLULA SKIKPDDVH--SKDEAKKMIVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGITCLT 770 sp|Q752B8|DPOE_ASHGO SQIDPEDKV--ARDEARKMIVLYDSLQLAHKVILNSFYGYAMRKGSRWYSIEMAGITCST 767 . ...: ::::******* ********.**:*:****:****:. * World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 576 DNA axis. Pol ε’s fidelity to a nascent Watson-Crick base-pairing was determined primarily by residues V825, N828, S829, Y831and G832 from the fingers’ domain (all the five amino acids are in the proposed catalytic core of the enzyme) and by Y645 from the palm domain. As discussed elsewhere, the Y831→A replacement in pol ε reduced replication fidelity and its participation in chromosomal replication, but without eliminating an additional function that is essential for viability [25]. It is interesting to note that the same invariant Y831G832 pair is proposed here as the template-binding pair by sequence similarity and is in close agreement with other DdDps and DdRps. The pol active site is characterized by the catalytic metal-binding residues, D640 and D877. Both (–HVD640and –DTD877-) are found to be completely conserved in the eukaryotic ε pols. The PR exonucleases active site amino acids are further confirmed by SDM and X-ray crystallographic data. When the D290 and E292 of the first triad in the PR exonuclease were mutated to A (highlighted in dark blue in Table 1), the exonuclease activity was abolished, confirming their direct involvement in PR function [15]. The x-ray crystallographic data further confirmed that the PR exo domain was located on the opposite side of the DNA of the thumb domain and contained the catalytic residues (D290, E292 and D477 and D275, E277 (numberings are based on the yeast and human enzymes, respectively) for PR activity [25]. A catalytic metal ion was found to be coordinated by D290 (D275 in human enzyme) (Fig. 5A). These active site amino acids are in complete agreement with the present MSA analysis (highlighted in light blue). Table 1 shows a comparative account of the active site amino acids in the PR exonuclease and pol domains of the eukaryotic DNA pol ε from different sources from yeasts to humans. The PR exonuclease and pol active sites are almost identical in all eukaryotes from lower (yeasts and higher fungi) to higher (plants and animals) except for a small difference where the plant and animal enzymes use a –KCpair at the proton abstraction site and the yeast and higher fungal enzymes use a –KVpair without altering the invariant K in all. The putative second pol active site amino acids are also identified in the CTD. However, the second PR exonuclease active site in the CTD is not complete in all ε pols (Table 1). Table 1 Proposed active site amino acids of PR exonuclease and pol domains of eukaryotic ε pols Adapted from Palanivelu [8]; Active site amino acids in dark blue are confirmed by SDM analysis; Mutations in pol ε PR exo active site region (highlighted in dark blue) lead to several types of cancers, e.g., D275 leads to endometrial, breast, glioblastoma, colorectal, lung cancers; F367 leads to endometrial, colon cancers; S459 leads to colon, endometrial, glioblastoma, duodenal cancers. The 3’→5’ PR exonucleolytic activity is intrinsic to the replicative DNA pols ε and δ and is essential for the faithful replication of the genomic DNA. Figs. 6A, B and C show the proposed active site amino acids at the PR exonuclease active sites of yeast, plant and human ε pols, respectively, which is based on the crystallographic, SDM and MSA data. Figures 6A, B and C. Proposed amino acids at the PR exonuclease active sites of DNA pols ε of S. cerevisiae (A), A thaliana (B) and humans (C) World Journal of Advanced Research and Reviews, 2025, 26(03), 545-578 577 The proposed two-metal ion in the active site in the PR exonucleases is based on Beese and Steitz’s observation of similar active site structure in the 3’→5’ PR exonuclease active site in E. coli pol I [28] and is also further confirmed from a large number DdRps [6]. 5. Conclusions Understanding the functions of the replicative pols, viz. α, ɛ and δ, which make the replisome complex, is a prerequisite for understanding the mechanism of genome replication and maintenance of genome integrity in eukaryotes. The present study unravels striking similarities of one of the main replicative pols ɛ from lower to higher eukaryotes at the molecular level. The MSA analysis has shown that the ε pols in all eukaryotes contain the same template-binding pairs, catalytic and nucleotide selection amino acids and also the same catalytic metal-binding motifs. Furthermore, the two completely conserved ZBMs in the CTDs of all the three replicative pols suggest their essential roles in genome replication and regulation. A unique [4Fe-4S]2+ cluster-binding motif in the ε pol domain is implicated in the redox signaling and the regulation of the replication process in eukaryotes as this enzyme links the DNA replication machinery to S-phase checkpoint-control in eukaryotes. Moreover, the PR exonuclease active site amino acids are identical in all eukaryotic enzymes and belong to the DEDD(Y) superfamily of PR exonucleases. These findings establish that the pol, PR exonuclease and CTD domains of the ε pols and their roles in genome replication, maintenance of genome integrity and regulation are highly conserved across all eukaryotes, from yeasts to higher animals. The highly conserved pol, PR exonuclease and CTD domains strongly suggest a possible common evolutionary origin of the ε pols in eukaryotes. Compliance with ethical standards Acknowledgments The author wishes to thank Dr. P. Sathiyamoorthy, Chief Scientist, GEM Research Foundation, Chennai, for critical evaluation of the manuscript. Disclosure of conflict of interest The author has declared that no competing interests exist. References [1] Palanivelu P. Analyses of priming reactions and proofreading functions during initiation of replication of prokaryotic and eukaryotic genomes. Br J Pharm Med Res. 2022; 7:3790-7828. [2] Palanivelu P. 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(2014) An Iron-Sulfur Cluster in the Polymerase Domain of Yeast DNA Polymerase epsilon. J Mol Biol. 2014; 426:301–308. [24] Pinto MN, Beek JT, Ekanger LA, Johansson E, Barton JK. The [4Fe-4S] Cluster of yeast DNA polymerase ε is redox active and can undergo DNA-mediated signaling. J Am Chem Soc. 2021: DOI: 10.1021/jacs.1c07150. [25] Jain R, Rajashankar KR. Buku A, Johnson RE, Prakash L, Prakash S, et al. Crystal structure of yeast DNA polymerase epsilon catalytic domain. PLoS ONE. 2014; 9:e94835. doi: 10.1371/journal.pone.0094835. [26] Jain R, Rice WJ, Malik R, Johnson RE, Prakash L, Prakash S, et al. Cryo-EM structure and dynamics of eukaryotic DNA polymerase δ holoenzyme. Nat Struct Mol Biol. 2019; 26:955–962. [27] Ohya T, Kawasaki Y, Hiraga S, Kanbara S, Nakajo K, Nakashima N, Suzuki A, Sugino A. The DNA Polymerase Domain of pol ε Is Required for Rapid, Efficient, and Highly Accurate Chromosomal DNA Replication, Telomere Length Maintenance, and Normal Cell Senescence in Saccharomyces cerevisiae. J Biol Chem. 2002; 277:28099– 28108 [28] Beese LS, Steitz TA. Structural basis for the 3’-5’ exonuclease activity of Escherichia coli DNA polymerase I: a twometal ion mechanism. EMBO J. 1991; 10:25–33.