A biological activity of Cicerbita alpina (L.) leaf extracts

A biological activity of Cicerbita alpina (L.) leaf extracts Mariya Rogova, Nikol Kalinova, Desislava Mantovska, Ana-Mariya Nedelcheva, Detelina Petrova, Dimitar Danchev, Miroslava Zhiponova Department of Plant physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, Sofia, Bulgaria e-mail: mrogov[email protected] Cicerbita alpina (L.) Wallr. is a perennial plant, common in the alpine parts of the mountains of the Republic of Bulgaria. The plant is used in the traditional cuisine of some countries such as Italy and Scandinavia, as well as in traditional folk medicine of many countries for the treatment of various diseases, including infectious diseases. Aim: The aim of the study was to investigate the anti-inflammatory and antimicrobial activity of the leave extracts from Cicerbita alpina, growing on Vitosha Mountain, Republic of Bulgaria, using the haemolytic complement method and the disk diffusion method. Results: The study showed that methanol extracts had higher antihaemolytic activity than chloroform extracts. The methanol extracts showed bactericidal activity against most Gram-positive bacterial strains and a bacteriostatic effect to the Gram-negative bacteria - A. calcoaceticus and E. cloacae.No antibacterial activity of the chloroform extracts towards the tested microorganisms was recorded. Conclusion: In conclusion, the leave methanol extracts showed better anti-inflammatory and antimicrobial activity compared to chloroform extracts, which is probably due to the polar compounds contained in them. Gram-positive (green colour) and Gramnegative (yellow colour) bacterial strains Inhibition zone (mm) Methanol extracts (5 mg/ml) Chloroform extracts (5 mg/ml) B. subtilis (A) 14 BC - B. cereus - - S. aureus (B) 12 BC - E. faecalis (C) 10 BC - S. epidermidis (D) 10 BC - P. aeruginosa - - K. pneumoniae - - E. cloacae (E) 13 BS - A. calcoaceticus (F) 10 BS - AB CD EF A B Fig. 1 Graphs of anti-inflammatory activity by the Complement method for chloroform (A) and methanol extracts (B) with indexed IC50 IC50=1732.741 IC50=1344.953 Table 1. Antibacterial activity of methanol and chloroform Alpine cicerbita leaf extracts against G (+) and G (-) bacteria