HPLC analysis of sugar content in agricultural waste hydrolysates – a step towards the sustainable synthesis of bioactive compounds

Plovdiv, Bulgaria, 30-31 October HPLC analysis of sugar content in agricultural waste hydrolysates – a step towards the sustainable synthesis of bioactive compounds Apostol Apostolov, Greta Naydenova, Dragomir Yankov Bulgarian Academy of Sciences, Institute of Chemical Engineering, Laboratory of Biochemical Engineering 6th balkan.bio conference 2025 Additional information: Science project - Novel approaches for the selection and integration of BIOTICS (pro/post and metabiotics) into functional formulations for dietary supplements with high biological potential based on agro and industrial wastes. Head of project - Svetla Danova, prof, research field - lactic acid bacteria and probiotics Apostol Apostolov - chemical engineer, Ph.D. student, assistant *Contact info: [email protected], Institute of Chemical Engineering, Sofia, acad. G. Bonchev str., Bl.103, Sofia 1113, Bulgaria Acknowledgments: The authors gratefully acknowledge the National Recovery and Sustainability Plan its financial support via the contract ПВУ – 63 от 16.12.2024 г. /BG-RRP-2.017-0047-C01/. Inspiration: The following work is part of the project BioNutriAgro. The main goal is to convert agro and industrial wastes into functional formulations. Conversion of organic waste via acid hydrolysis yields essential compounds that are further used to produce pro-, meta-, and postbiotics. Method: High-performance liquid chromatography coupled with a refractive index detector is applied to analyse the sugar content after the acid hydrolysis of: spent coffee grounds (SCG); dried distilled grains with solubles; grain leftovers; sunflower seed press cake; sunflower seed husks. Material Sugars after Acid Hydrolysis (g/L) Spent Coffee Grounds Mannose (0.1), Galactose (7.23), Arabinose (0.1), Glucose (12.36), Oligosaccharides Dried Distilled Grains with Solubles Glucose (4.46), Xylose (0.82), Arabinose (0.89) Grain Leftovers Glucose (43.17), Xylose (2.67), Arabinose (3.39), Oligosaccharides Material Sugars after Acid Hydrolysis (g/L) Sunflower Seed Press Cake Glucose (33.64), Xylose (9.76), Arabinose (3.89), Oligosaccharides Sunflower Seed Husks Glucose (19.38), Xylose (3.73), Arabinose (1.61)