Supplemetary material: Soil application of urban waste-derived compost increased mi-crobial community diversity in the grapevine rhizosphere: a rhizobox approach

Appendix A – Supplementary material Supplementary Figure 1 - Analysis of similarities (ANOSIM) at species level of the amended soil analyzed (ACM, ACF, GDD) and the control (CK). Supplementary Table 1. Chemical-physical analysis of the soil used in the experiment Determination Unit Value Sandy % 20.5 Loam % 41.8 Clay % 37 pH - 7.2 EC mS cm-1 0.29 SOM % 1.52 C/N - 8.1 Total CaCO3 % 2.02 Active CaCO3 % 1.7 CEC Meq/100g 27.9 Total N g kg-1 1.09 P2O5 mg kg-1 47 K2O mg kg-1 262 Ca mg kg-1 6260 Mg mg kg-1 365 Na mg kg-1 421 Fe mg kg-1 19.6 Mn mg kg-1 23.4 Cu mg kg-1 5.4 Zn mg kg-1 1.7 Supplementary Table 2. Effect of the amendments on plant growth, total DW biomass, chlorophyll content, and root length. Treatment Leaf Area (cm²) Total leaves (n) Total dry biomass (g) Leaf chlorophyll (SPAD) Root length (LOG cm) Shoot Length (cm) CK 965 24.5 31.9 38.0 3.82 b 70.1 ACM 1033 23.5 30.1 38.7 4.03 ab 62.9 ACF 1008 24.5 28.2 37.8 3.91 ab 52.3 GDD 1138 29.3 28.6 39.3 4.1 a 57.1 Significance ns ns ns ns * ns ns, *: effect of treatment not significant or significant at p < 0.05, respectively. Values in the same column followed by the same letter are not statistically different according to the Student Neuwman-Keuls test (p < 0.05). Supplementary Table 3. Statistics of scaftigs (≥500 bp) SampleID Total len (bp) Scaftigs num Average len (bp) N50 len (bp) N90 len (bp) Max len (bp) ACF.1 222,413,970 267,242 832.26 755 531 173,935 ACF.2 221,498,110 286,418 773.34 713 526 62,066 ACF.3 236,997,084 323,915 731.66 687 524 36,788 ACF.4 162,444,944 201,783 805.05 731 527 61,312 ACM.1 214,248,976 276,148 775.85 716 527 30,130 ACM.2 185,129,046 244,638 756.75 698 525 33,864 ACM.3 307,938,492 326,665 942.67 900 543 194,993 ACM.4 322,665,793 346,245 931.9 886 540 105,737 GDD.1 274,163,445 310,473 883.05 813 538 639,185 GDD.2 348,052,630 416,217 836.23 766 533 299,996 GDD.3 301,995,332 375,786 803.64 735 530 105,035 GDD.4 257,535,072 314,712 818.32 750 530 107,349 CK.1 288,727,137 384,998 749.94 705 528 199,750 CK.2 357,453,814 471,130 758.72 714 529 163,955 CK.3 334,666,881 414,127 808.13 747 533 156,457 CK.4 170,647,550 202,069 844.5 782 534 58,988 Supplementary Table 4. QC statistics SampleID Raw Base (G) Clean Base(G) Clean_Q2 0(%) Clean_Q3 0(%) Clean_GC (%) Effective (%) NonHost _Base(G) ACF.1 6.91 6.77 97.42 93.1 63.79 98.04 6.75 ACF.2 7.24 7.08 95.84 89.17 64.56 97.75 7.08 ACF.3 7.95 7.78 96.14 89.92 64.65 97.83 7.77 ACF.4 5.96 5.83 95.78 88.99 64.48 97.76 5.83 ACM.1 6.84 6.69 95.79 88.93 64.37 97.85 6.69 ACM.2 6.4 6.26 95.83 89.07 64.62 97.77 6.26 ACM.3 6.69 6.56 95.29 87.69 64.9 97.99 6.56 ACM.4 7.54 7.35 95.68 88.79 64.82 97.45 7.32 GDD.1 7.15 7.02 97.63 93.56 63.15 98.24 7.02 GDD.2 8.7 8.51 95.84 89.14 65.07 97.89 8.51 GDD.3 8.52 8.33 95.84 89.17 65.24 97.8 8.32 GDD.4 7.29 7.16 95.92 89.22 64.97 98.16 7.16 CK.1 7.66 7.47 95.48 88.24 65.54 97.58 7.47 CK.2 8.88 8.67 95.88 89.37 65.28 97.66 8.66 CK.3 8.71 8.5 95.65 88.69 65.4 97.53 8.5 CK.4 6.09 5.95 95.43 88.19 64.03 97.76 5.93