Microbead-Encapsulated Luminescent Bioreporter Screening of P. aeruginosa via Its Secreted Quorum-Sensing Molecules

Citation: Paul, A.A.; Kadosh, Y.S.; Kushmaro, A.; Marks, R.S. Microbead-Encapsulated Luminescent Bioreporter Screening of P. aeruginosa via Its Secreted Quorum-Sensing Molecules. Biosensors 2024,14, 383. https://doi.org/10.3390/bios14080383 Received: 4 July 2024 Revised: 3 August 2024 Accepted: 5 August 2024 Published: 8 August 2024 Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). biosensors Article Microbead-Encapsulated Luminescent Bioreporter Screening of P. aeruginosa via Its Secreted Quorum-Sensing Molecules Abraham Abbey Paul 1, Yael Schlichter Kadosh 1, Ariel Kushmaro 1,2,3 and Robert S. Marks 1,2,* 1 Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Be’er Sheva 84105, Israel; [email protected] (A.A.P.); [email protected] (Y.S.K.); [email protected] (A.K.) 2The Ilse Katz Center for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Be’er Sheva 84105, Israel 3School of Sustainability and Climate Change, Ben-Gurion University of the Negev, Be’er Sheva 84105, Israel *Correspondence: [email protected]; Tel.: +972-547391291 Abstract: Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium that remains a prevalent clinical and environmental challenge. Quorum-sensing (QS) molecules are effective biomarkers in pinpointing the presence of P. aeruginosa. This study aimed to develop a convenient-to-use, whole-cell biosensor using P. aeruginosa reporters individually encapsulated within alginate-poly-Llysine (alginate-PLL) microbeads to specifically detect the presence of bacterial autoinducers. The PLL-reinforced microbeads were prepared using a two-step method involving ionic cross-linking and subsequent coating with thin layers of PLL. The alginate-PLL beads showed good stability in the presence of a known cation scavenger (sodium citrate), which typically limits the widespread applications of calcium alginate. In media containing synthetic autoinducers—such as N-(3-oxo dodecanoyl) homoserine lactone (3-oxo-C 12 -HSL) and N-butanoyl-L-homoserine lactone (C 4 -HSL), or the cell-free supernatants of planktonic or the flow-cell biofilm effluent of wild P. aeruginosa (PAO1)—the encapsulated bacteria enabled a dose-dependent detection of the presence of these QS molecules. The prepared bioreporter beads remained stable during prolonged storage at 4 and −80 ◦C and were ready for on-the-spot sensing without the need for recovery. The proof-of-concept, optical fiber-based, and whole-cell biosensor developed here demonstrates the practicality of the encapsulated bioreporter for bacterial detection based on specific QS molecules. Keywords: alginate; microbeads; optical biosensors; bioencapsulation; autoinducers; Pseudomonas aeruginosa; quorum sensing; poly-lysine; hydrogels; C 4 -HSL; 3-oxo-C 12 -HSL; Furanone C-30; QS inhibitor; whole-cell biosensors 1. Introduction Pseudomonas aeruginosa is an increasingly prevalent opportunistic Gram-negative bacterium that causes nosocomial and life-threatening infections of immunocompromised [ 1 ] and cystic fibrotic patients [ 2 ]. Many Gram-negative bacteria regulate the expression of specialized gene sets in response to their population density by autoinduction, in which small molecules called autoinducers (AIs) are produced and can diffuse freely across the bacterial cell wall [ 3 ]. In response to the levels of AIs, individual cells can determine whether the population density is high enough to initiate the expression of a particular phenotype. This type of gene regulation is termed quorum sensing (QS) [3]. The study of QS has emerged as a key research area in the past two decades due to its involvement in vital microbial activities. For instance, bacterial swarming and motility, sporulation, biofilm formation, bioluminescence, and the production of virulence factors [ 4 ] are regulated by QS. Moreover, about 80% of microbial infections have been associated with biofilm formation, and the contribution of QS molecules in biofouling is well established. N-acyl homoserine lactones (AHLs) are used by P. aeruginosa as autoinducers for quorum Biosensors 2024,14, 383. https://doi.org/10.3390/bios14080383 https://www.mdpi.com/journal/biosensors Biosensors 2024,14, 383 2 of 22 sensing. The length of the fatty acyl group in the AHLs varies from 4 to 14 carbons, and the group can be either fully reduced, have a 3-oxo or 3-hydroxyl group, or have an unsaturated bond. P. aeruginosa signal molecules–N-butyryl-homoserine lactone (C 4 -HSL) and N-3-oxododecanoyl-homoserine lactone (3OC 12 -HSL) have been detected in clinical samples of cystic fibrosis patients [5–8], with positive correlations found with the infection rate [9]. A highly sensitive detection of AHLs can be achieved by biosensors and liquid and gas chromatographic methods [ 10 , 11 ]. Although such physical–chemical techniques can be sensitive, their widespread use is hampered by the need for solvent extraction, derivatization of the analytes, and the cost of the instrument, as well as the requirement for trained personnel. Consequently, biosensors have shown to be viable alternatives due to their affordable cost, fast analysis time, and high sensitivity, thus making them ideal for routine testing and the screening of samples [ 10 ]. The bioreporter bacteria strain can detect quorum-sensing molecules because they are genetically rendered incapable of producing AHLs themselves but maintaining their capability of sensing their presence. Such genetic mutations are usually introduced upstream of a LuxR-controlled promoter, and they are fused to a gene coding for an easily detectable output signal. For details on the construction of bacterial lux-biosensors, readers are directed to the review work of Bazhenov et al. [12]. In this work, the bioluminescent reporter gene (luxCDABE) is fused downstream of the luxR-promoter. Among cell-based biosensors, the luxCDABE reporter is reputed for the lowest detection limits (down to 10 −18 mol) it offers [ 13 ]. The luxA and luxB genes within the luxCDABE gene cassette encode for bacterial luciferase, while the luxC, luxD, and luxE genes in the same cassette encode for the enzymes required for the synthesis and recycling of the luciferase substrate. This setup eliminates the need for any external addition of the substrate, enabling continuous real-time monitoring with low background interferences [ 12 ]. Transcription is initiated by binding the AHL–luxR complex to the promoter, leading to concomitant luminescent production (Figure 1). The amount of luminescence generated can be correlated with the concentration of the standard AHLs from which the calibration curve is generated. This can be used to quantitatively determine the presence of the quorumsensing molecules in test/unknown samples. Our research advanced the development of whole-cell sensing systems by immobilizing them through encapsulation in reinforced alginate beads. These beads can be utilized as reporter microbeads for monitoring the AHLs in biological and environmental samples, including culture supernatants and biofilm flowthroughs. Whole-cell biosensing systems possess attributes that make them ideal for portable field kits. These attributes include the capacity to withstand a wide range of environmental conditions (such as various temperatures, pH levels, and ionic strengths), providing information on analyte bioavailability and requiring minimal or no sample pretreatment. Additionally, they are highly sensitive, selective, are easily prepared, provide rapid detection, are cost-effective, and are amenable to high-throughput screening and miniaturization [14]. Hydrogels are water-retaining and water-insoluble three-dimensional cross-linked polymeric networks. Sodium alginates are naturally occurring polyanionic polysaccharides comprising a linear copolymer of 1 → 4-linked β -D-mannuronic acid (M units) and α -L-guluronic acid (G units). Sodium alginate possesses a unique ability to chelate with multivalent cations, such as divalent ions like Ca 2+ and Ba 2+ in solution via the carboxylic acid groups of the G-G blocks, to form insoluble calcium alginate with an “egg-box” molecular structure [ 15 ]. Owing to this rapid and nontoxic preparation approach, alginate has emerged as a choice biomaterial for various encapsulation applications [ 16 , 17 ]. Calcium alginate hydrogel is unstable in physiological solutions and the presence of cation scavengers such as citrate, ethylene diamine tetra acetic acid, and phosphates. The calcium ions can, therefore, be exchanged by other ions, resulting in a loss of cross-links from the alginate gels. This makes the gels structurally and mechanically weaker, leading to uncontrollable permeability and eventual loss of the stable 3D structure [ 15 ]. This effect has continued to limit the widespread applications of calcium alginate hydrogels. Therefore, to address the above problem, efforts have been made to increase the chemical and physical durability Biosensors 2024,14, 383 3 of 22 of alginate gels by coating them with cationic and anionic polymers such as chitosan, as well as by exploiting several covalent cross-linking strategies and a composite-based approach [ 15 , 18 ]. To that effect, in this study, poly-lysine-reinforced calcium alginate was used to encapsulate the bioreporter in a two-step approach. Although Strand et al. 2003 employed confocal laser scanning microscopy (CLSM) to characterize the microcapsules of alginate-poly-L-lysine in terms of layer formation, stability, and distribution [ 19 ], the biotechnological applications of these capsules have been sparingly employed in drug release experiments [16] and cell encapsulation studies [20]. Biosensors 2024, 14, x FOR PEER REVIEW 3 of 23 Figure 1. Schematic of the cellular events that result in the expression of a reporter protein. The cell can easily take up a bioavailable analyte, such as AHL, through its membrane. AHL (3-oxo-C 12 -HSL and C 4 -HSL) binds with a regulatory protein called LasR or RhlR, forming a protein–AHL complex. This complex binds to its corresponding promoter, Plasl or Prhl, which triggers activation of the transcription and translation of a reporter gene called luxCDABE. This results in the expression of the reporter protein, luciferase, inducing the bioluminescent reaction characterized by the emission of blue-green light (~490 nm). Created with BioRender.com, accessed on 13 June 2024. Hydrogels are water-retaining and water-insoluble three-dimensional cross-linked polymeric networks. Sodium alginates are naturally occurring polyanionic polysaccharides comprising a linear copolymer of 1 → 4-linked β-D-mannuronic acid (M units) and α-L-guluronic acid (G units). Sodium alginate possesses a unique ability to chelate with multivalent cations, such as divalent ions like Ca 2+ and Ba 2+ in solution via the carboxylic acid groups of the G-G blocks, to form insoluble calcium alginate with an “egg-box” molecular structure [15]. Owing to this rapid and nontoxic preparation approach, alginate has emerged as a choice biomaterial for various encapsulation applications [16,17]. Calcium alginate hydrogel is unstable in physiological solutions and the presence of cation scavengers such as citrate, ethylene diamine tetra acetic acid, and phosphates. The calcium ions can, therefore, be exchanged by other ions, resulting in a loss of cross-links from the alginate gels. This makes the gels structurally and mechanically weaker, leading to uncontrollable permeability and eventual loss of the stable 3D structure [15]. This effect has continued to limit the widespread applications of calcium alginate hydrogels. Therefore, to address the above problem, efforts have been made to increase the chemical and physical durability of alginate gels by coating them with cationic and anionic polymers such as chitosan, as well as by exploiting several covalent cross-linking strategies and a composite-based approach [15,18]. To that effect, in this study, poly-lysine-reinforced calcium alginate was used to encapsulate the bioreporter in a two-step approach. Although Strand et al. 2003 employed confocal laser scanning microscopy (CLSM) to characterize the microcapsules of alginate-poly-L-lysine in terms of layer formation, stability, and distribution [19], the biotechnological applications of these capsules have been sparingly employed in drug release experiments [16] and cell encapsulation studies [20]. This work aimed to develop an alginate-based bioreporter encapsulation for detecting quorum-sensing molecules with a rationally designed coating developed through Figure 1. Schematic of the cellular events that result in the expression of a reporter protein. The cell can easily take up a bioavailable analyte, such as AHL, through its membrane. AHL (3-oxo-C 12 -HSL and C 4 -HSL) binds with a regulatory protein called LasR or RhlR, forming a protein–AHL complex. This complex binds to its corresponding promoter, Plasl or Prhl, which triggers activation of the transcription and translation of a reporter gene called luxCDABE. This results in the expression of the reporter protein, luciferase, inducing the bioluminescent reaction characterized by the emission of blue-green light (~490 nm). Created with BioRender.com, accessed on 13 June 2024. This work aimed to develop an alginate-based bioreporter encapsulation for detecting quorum-sensing molecules with a rationally designed coating developed through polylysine assembly. In brief, our approach involves sending a feed solution of alginate and engineered P. aeruginosa (PAO-JP2) strains through a microcapillary (which is connected to an airstream) into a reservoir containing Ca 2+ to form calcium alginate microbeads (Figure 2). The resulting PAO-JP2-alginate microbeads were incubated in a PLL solution, resulting in a core–shell structure with a core of alginate that is cross-linked by Ca 2+ and a thin shell of PLL that forms electrostatic interactions between the carboxyl of alginate and the ε -amino groups of PLL. The thin polymer shell around the alginate core stabilizes the overall microcapsule. Even if the alginate core became degraded due to ion exchange or chelation (in citrate or other solution), the microcapsule remains intact thanks to the PLL membrane. Biosensors 2024,14, 383 4 of 22 Biosensors 2024, 14, x FOR PEER REVIEW 4 of 23 poly-lysine assembly. In brief, our approach involves sending a feed solution of alginate and engineered P. aeruginosa (PAO-JP2) strains through a microcapillary (which is connected to an airstream) into a reservoir containing Ca 2+ to form calcium alginate microbeads (Figure 2). The resulting PAO-JP2-alginate microbeads were incubated in a PLL solution, resulting in a core–shell structure with a core of alginate that is cross-linked by Ca 2+ and a thin shell of PLL that forms electrostatic interactions between the carboxyl of alginate and the ε-amino groups of PLL. The thin polymer shell around the alginate core stabilizes the overall microcapsule. Even if the alginate core became degraded due to ion exchange or chelation (in citrate or other solution), the microcapsule remains intact thanks to the PLL membrane. Figure 2. Schematics of the microcapsule synthesis. The size of the beads can be controlled by changing the flow rate of the feed solution (alginate + bioreporter) and the air pressure. The above procedure was used to encapsulate bioreporter bacteria and allow the free passage of exogenously added small quorum-sensing (QS) molecules that elicit a dosedependent biochemical response from the bioreporter (Figure 1). The prepared bioreporter beads demonstrated biosensing ability toward both synthetic and secreted autoinducers. Furthermore, the bioreporter beads effectively detected the presence of a synthetic quorum-sensing inhibitor, furanone C-30. These beads exhibited excellent storability in all tested conditions, requiring no additional preparation before use. As a proof of concept, the bioreporters in this study were employed to develop optical fiber-based, whole-cell biosensors using the established optical setup developed in our laboratory [21–24]. The encapsulated bioreporters serve as the recognition element, the optical fiber as the transducer, and the photomultiplier tube as the detector in a compact, light–tight ‘black box’ (Figures 3 and S19). This makes the microbeads suitable as an ondemand capsule for potential wide application in monitoring and diagnostics. Figure 2. Schematics of the microcapsule synthesis. The size of the beads can be controlled by changing the flow rate of the feed solution (alginate + bioreporter) and the air pressure. The above procedure was used to encapsulate bioreporter bacteria and allow the free passage of exogenously added small quorum-sensing (QS) molecules that elicit a dosedependent biochemical response from the bioreporter (Figure 1). The prepared bioreporter beads demonstrated biosensing ability toward both synthetic and secreted autoinducers. Furthermore, the bioreporter beads effectively detected the presence of a synthetic quorumsensing inhibitor, furanone C-30. These beads exhibited excellent storability in all tested conditions, requiring no additional preparation before use. As a proof of concept, the bioreporters in this study were employed to develop optical fiber-based, whole-cell biosensors using the established optical setup developed in our laboratory [ 21 – 24 ]. The encapsulated bioreporters serve as the recognition element, the optical fiber as the transducer, and the photomultiplier tube as the detector in a compact , light–tight ‘black box’ (Figures 3and S19). This makes the microbeads suitable as an on-demand capsule for potential wide application in monitoring and diagnostics. Biosensors 2024, 14, x FOR PEER REVIEW 5 of 23 Figure 3. The basic components of the whole-cell, fiber-optic biosensors. (A) Optical fiber with a bioreporter immobilized unto the core. (B) Light–tight portable black box encasing all the biosensor components. For the image of the black box, readers are directed to the Supplementary Materials section (Figure S19). 2. Materials and Methods 2.1. Materials Sodium alginate (low viscosity, A2158), trimethoprim, poly-l-lysine, poly-D-lysine, calcium chloride, Luria–Bertani medium (LB broth), and LB agar were purchased from Becton (Dickinson & Company, Le Pont de Claix, France) . Acyl-homoserine lactone (acylHSL) 3-oxo-C 12 -HSL, C 4 -HSL, C 6 -HSL, 3-oxo-C 8 -HSL, 3-oxo-C 6 -HSL, and (as in Figure S2) the stock (5 mM) solutions were stored at −20 °C until required, and, as well as QS inhibitor—furanone C-30, were purchased from Sigma-Aldrich (St. Louis, MO, USA). Difco Luria–Bertani (LB) broth, Miller (10 g of L −1 tryptone; 5 g of L −1 yeast extract; and 10 g of L −1 NaCl) and Difco LB agar, and Miller agar (10 g of L −1 tryptone; 5 g of L −1 yeast extract; 10 g of L −1 NaCl; and 15 g of L −1 agar) were obtained from Becton (Dickinson & Company, Le Pont de Claix, France). The LIVE/DEAD TM BacLight TM Bacterial Viability Kit was obtained from Invitrogen, Thermo Fisher Scientific, Waltham, MA USA. Bacteria Strains LasR and RhlR reporters—P. aeruginosa PAO-JP2-luxCDABE and PAO-JP2 (pKDrhlA) QS reporters PAO-JP2 (pKD201-lasI) is a double mutant (lasI/rhlI-deleted) strain of P. aeruginosa PAO1. It harbors a plasmid pKD201 with a lasI promoter coupled upstream to the luxCDABE luminescence system operon, which is responsive to 3OC12-HSL for LasR activity detection, as described previously by Ganin et al. [25]. PAO-JP2 (pKD-rhlA) is a lasIrhlI double mutant of Pseudomonas aeruginosa PAO1 that harbors a pKD vector with a rhlA promoter coupled upstream to the luxCDABE operon. K. Duan et al. [26] developed the reporter strain responsive to C 4 -HSL (Figure S1). The bacteria strains were obtained from Prof. Michael Meijler, M. M. Ben-Gurion University of the Negev, Be’er Sheva, Israel. The scheme of the plasmid is presented in the Supplementary Materials, Figure S1. 2.2. Synthesis of Microcapsules The microbead synthesis device was fabricated similarly to what Ahn et al. described [27] The feed solution was 2% (w/v) sodium alginate, and the reservoir was made of 150 mM of CaCl 2 in water. The feed was loaded into a syringe and fed through the capillary device mentioned above, with the feed flow rate typically being 30 mL/h. The feed Figure 3. The basic components of the whole-cell, fiber-optic biosensors. (A) Optical fiber with a bioreporter immobilized unto the core. (B) Light–tight portable black box encasing all the biosensor components. For the image of the black box, readers are directed to the Supplementary Materials section (Figure S19). Biosensors 2024,14, 383 5 of 22 2. Materials and Methods 2.1. Materials Sodium alginate (low viscosity, A2158), trimethoprim, poly-l-lysine, poly-D-lysine, calcium chloride, Luria–Bertani medium (LB broth), and LB agar were purchased from Becton (Dickinson & Company, Le Pont de Claix, France). Acyl-homoserine lactone (acylHSL) 3-oxo-C 12 -HSL, C 4 -HSL, C 6 -HSL, 3-oxo-C 8 -HSL, 3-oxo-C 6 -HSL, and (as in Figure S2) the stock (5 mM) solutions were stored at − 20 ◦ C until required, and, as well as QS inhibitor—furanone C-30, were purchased from Sigma-Aldrich (St. Louis, MO, USA). Difco Luria–Bertani (LB) broth, Miller (10 g of L −1 tryptone; 5 g of L −1 yeast extract; and 10 g of L −1 NaCl) and Difco LB agar, and Miller agar (10 g of L −1 tryptone; 5 g of L −1 yeast extract; 10 g of L −1 NaCl; and 15 g of L −1 agar) were obtained from Becton (Dickinson & Company, Le Pont de Claix, France). The LIVE/DEAD TM BacLight TM Bacterial Viability Kit was obtained from Invitrogen, Thermo Fisher Scientific, Waltham, MA USA. Bacteria Strains LasR and RhlR reporters—P. aeruginosa PAO-JP2-luxCDABE and PAO-JP2 (pKD-rhlA) QS reporters. PAO-JP2 (pKD201-lasI) is a double mutant (lasI/rhlI-deleted) strain of P. aeruginosa PAO1. It harbors a plasmid pKD201 with a lasI promoter coupled upstream to the luxCDABE luminescence system operon, which is responsive to 3OC12-HSL for LasR activity detection, as described previously by Ganin et al. [ 25 ]. PAO-JP2 (pKD-rhlA) is a lasI-rhlI double mutant of Pseudomonas aeruginosa PAO1 that harbors a pKD vector with a rhlA promoter coupled upstream to the luxCDABE operon. K. Duan et al. [ 26 ] developed the reporter strain responsive to C 4 -HSL (Figure S1). The bacteria strains were obtained from Prof. Michael Meijler, M. M. Ben-Gurion University of the Negev, Be’er Sheva, Israel. The scheme of the plasmid is presented in the Supplementary Materials, Figure S1. 2.2. Synthesis of Microcapsules The microbead synthesis device was fabricated similarly to what Ahn et al. described [ 27 ] The feed solution was 2% (w/v) sodium alginate, and the reservoir was made of 150 mM of CaCl 2 in water. The feed was loaded into a syringe and fed through the capillary device mentioned above, with the feed flow rate typically being 30 mL/h. The feed solution was converted into droplets using an air-driven droplet generator (Figure 2). As previously described, droplets were sheared off from the capillary tip by pulses of compressed air into an unstirred reservoir solution; after that, they were cross-linked into capsules. The capsules were allowed to cross-link for 10 min, after which they were washed and stored in double-distilled water (DDW) for subsequent steps. 2.2.1. Bacteria Strain Growth Conditions All cells involved in encapsulation and characterization experiments were PAO-JP2 (pKD201-lasI) and PAO-JP2 (pKD-rhlA), abbreviated as LasR and RhlR, respectively. The glycerol stocks of reporter strains PAO-JP2 (pKD201-lasI) or PAO-JP2 (pKD-rhlA) were stored at − 80 ◦ C. The stocks were streaked on to an LB agar plate containing 300 µ g/mL of trimethoprim (TMP), incubated at 37 ◦ C overnight in an incubator (Binder, Camarillo, CA, USA) to form colonies, and then stored in the refrigerator for less than a month. Starter cultures of LasR or RhlR were prepared by individually introducing a single colony into 10 mL of LB (Difco Lenox medium, BD, Le Pont de Claix, France) broth, supplemented with 300 µ g mL −1 of TMP (Sigma-Aldrich, MO, USA), grown overnight in a shaking incubator (New Brunswick™ Innova ® 44/44R Shaker) at 37 ◦ C, and shook at 220 rpm to attain an exponential growth phase. The fast-growing bacteria were reinoculated into fresh LB broth in a 1:50 dilution and grown to an approximate optical density of 0.6–0.9 at 600 nm (OD 600 ). Then, a determined volume of the bacteria culture was pelleted in a sterile 50 mL tube by centrifugation at 4 ◦ C and 3000 rpm for 15 min. The supernatant was discarded while the cell pellet was resuspended in 1000–2000 µL of LB and refrigerated until use. Biosensors 2024,14, 383 6 of 22 2.2.2. Encapsulation of Bacteria in Single-/Multiple-Layer Microcapsules Poly-L/D-lysine-stabilized, bacteria-loaded alginate microbeads were prepared using a simple two-step approach similar to the method used in [ 28 ]. First, 1 mL of the diluted bacteria was added to a 4 mL portion of 2.5% w/valginate solution (resulting in 2% w/v alginate solution), and this was then mixed well using a revolver tube rotator. The alginate bacteria solution was extruded into 150 mM of CaCl 2 under a controlled extrusion flow rate and air pressure using the above-described capillary setup (Figure 2), and the resulting microbeads were allowed to harden for 10 min and rinsed with sterile double-distilled water (DDW). The microbeads were incubated in 2 mL of a 0.05% w/vsolution of PLL (or PDL) for 10 min to allow the formation of a thin layer around the microbead, followed by washing with DDW. The resulting bacteria-alginate/PLL microbeads were subjected to 10 min incubation in 0.05% w/valginate to form an outer layer on the capsules and to neutralize the non-reacted PLL, creating a negatively charged surface, and then rinsed again. This standard encapsulation approach was adopted in all subsequent experiments unless otherwise stated. For double-membrane microbeads, the same procedure was followed as for the singlemembrane microbeads, except that the PLL incubation time was 7.5 min. After the alginate step, the microcapsules were incubated for 7.5 min in 2 mL of 0.05% PLL, which were then rinsed, incubated in 0.05% alginate for 10 min, and then the rinsed again, resulting in double-membrane microbeads. The triple-membrane microbeads were prepared in the same way as the doublemembrane microbeads. After the 0.05% w/valginate step, the double-membrane microbeads were incubated in 2 mL of 0.05% w/vPLL for 5 min and washed. The microbeads were then coated again with a 0.05% w/valginate solution. The size (diameter) of the beads was determined using a standard bright-field microscope, a 2.5 × objective lens, and ImageJ 1.54f. Alginate-poly-lysine and alginate-alone beads were tested for stability in the presence of a Ca2+ ion chelator and sodium citrate. 2.3. Structural Characterization 2.3.1. X-ray Photoelectron Spectroscopy (XPS) Analysis X-ray photoelectron spectroscopy (XPS) analysis was conducted on the dehydrated microcapsules, beads, alginate, and PLL films using an “ESCALAB Xi+” instrument by Thermo-Fisher Scientific. The ionization energies of the various elements present in the samples were measured. The analysis was performed under an ambient pressure of <1 × 10 −10 mbar. Aluminum K α radiation source (1486.68 eV) was used for photoelectron emission at room temperature, and the spectra were collected at a 90 ◦ angle from the X-ray source. A low-energy electron flood gun was employed to minimize surface charging, and the measurements were taken with a spot diameter of 650 µ m. Spectral analysis was carried out using the Avantage software version 6.6.0 provided by Thermo-Fisher Scientific. High-resolution spectra of C1s, O1s, N1s, and Ca1s peaks and a survey spectrum at 20 eV pass energy were obtained. 2.3.2. Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) Analyses The outer portion of alginate-PLL microcapsules was examined using Smart iTR™ Diamond ATR-FTIR sampling accessory and Nicolet 6700 spectrometer to examine the interactions between the molecules within the alginate-PLL microcapsule membrane. All measurements were taken on the built-in diamond ATR crystal. The FTIR spectra were recorded over the range of 4000–650 cm −1 at 4 cm −1 resolutions and represented an average of 36 scans. The spectrum of the clean dry diamond ATR crystal in the ambient atmosphere (air) was used as the background for infrared measurement. 2.3.3. Scanning Electron Microscopy (SEM) Scanning electron microscopy was performed on the double-layer hydrogels (freezedried to maintain the porous structure without any collapse) to obtain information on Biosensors 2024,14, 383 7 of 22 the external structure of PLL-coated and non-coated alginate hydrogels. The sufficiently swollen hydrogels were removed from the solution, quickly frozen at − 86 ◦ C for 2 h, and then freeze-dried under a vacuum at room temperature for 3 days to remove the imbibed water completely until the samples became completely dry prior to morphological observation. The freeze-dried hydrogels were mounted onto the base plate and coated with gold (~10 nm). The morphology of the hydrogels at room temperature was observed using a field-emission scanning electron microscope (FESEM, Thermo Fisher Verios 460L) at different magnifications. 2.4. Bioluminescent Assay The bioluminescent response of the alginate-poly-lysine immobilized bioreporter PAOJP2 (pKD201-lasI) and PAO-JP2 (pKD-rhlA) strains were acquired throughout a 20–30 h period using the Luminoskan Ascent Luminometer (Thermo Fisher Scientific, Waltham, MA, USA) and were maintained at 37 ◦ C. The luminometer was set to a kinetic mode at 5 or 10 min intervals between readings. White (non)-transparent, 96-well, and flat-bottom microplates (Nunc, Roskilde, Denmark) was used in all experiments. The experiments were carried out in three/four biological replicates with at least two independent experiments. The maximum luminescence values (expressed in the relative luminescent unit, RLU) were extracted using Microsoft Office Excel, from where the induction factor was calculated as a ratio of the maximum RLU of the test to that of the negative control (in the absence of an inducer). 2.4.1. Viability Assay of the Immobilized Bacterial Strains Microcapsules containing LasR and RhlR strains were placed in a sterile 24-well plate containing LB broth and were mildly shaken for about 25 h in an orbital shaker in a hot room maintained at 30 ◦ C. For live/dead staining, capsules were washed with saline solution (0.9% w/vNaCl) three times. After washing, capsules were incubated at room temperature for 15 min in a saline solution containing a propidium iodide (dead) and SYTO™ 9 (live) stain washed with saline, and these were then transferred to an ibid 12-well plate for confocal microscope imaging. 2.4.2. The Detection of Synthetic AHLs (3-oxo-C12-HSL and C4-HSL) The ability of the immobilized reporter bacteria to detect the sub-nanomolar concentration of the inducer was assessed by incubating the bioreporter microbeads in the presence of a known concentration of the inducers. Typically, 3–10 microbeads were added to each well in a white non-transparent, 96-well, and flat-bottom microplate (Nunc, Roskilde, Denmark), followed by the addition of 190 µL of LB broth (containing 300 µ g/mL of Trimethoprim) incubated at 37 ◦ C with/without shaking at 220 rpm for a specified period. Dedicated calibration curves were obtained by adding 10 µ L of the QS molecules to the well containing the microbeads at a range of final concentrations, 5 µ M–500 pM of 3-oxo-C 12 -HSL (for the LasR reporter), and 10 µ M–100 pM of C 4 -HSL (for RhlR reporter). The plate was incubated for 20–30 h at 37 ◦ C, and, during this time, luminescence measurements were performed at 5 or 10 min intervals using a Luminoskan ascent multi-mode reader luminometer (Thermo Scientific, Shanghai, China) and another luminometer (Luminoskan, Thermo Fisher Scientific, Waltham, MA, USA). The equations derived from the calibration curves were used to calculate the concentration of the 3OC 12 -HSL and C 4 -HSL present in both culture supernatant and biofilm flow-through samples. All the experiments were carried out in three or four replicates and repeated at least twice. Biosensors 2024,14, 383 8 of 22 2.4.3. Detection of AHLs in the Supernatant of Wild Cultures of PAO1 Strains A bioreporter was employed to detect the presence of the QS molecules in the supernatants of the culture of a wild strain of P. aeruginosa. The overnight culture of PAO1 was briefly reinoculated and grown overnight in a shaking incubator (New Brunswick™ Innova ® 44/44R Shaker) at 37 ◦ C and shook at 220 rpm until the OD 600 was about 0.9. Then, a determined volume of the bacteria culture was pelleted by centrifugation at 4 ◦ C and 10,000 × gfor 10 min. The supernatant was filtered through a 0.22 µ m-membrane filter and used as a source of AHLs for the biosensor testing. 2.4.4. The Detection of AHLs in the Biofilm Effluent Obtained in the Flow-Cell Experiment The biofilm experiment was carried out under flow conditions using a standard method, as shown in the following Supplementary Materials scheme (Protocol S1 and Figure S12) according to Goldberg et al. [ 29 ]. The flow was allowed to continue undisturbed for 12, 24, 48, and 72 h, and the flow through (effluent) at the determined time was collected, filtered using a 0.22 µ m-membrane filter, and stored at − 80 ◦ C until required. The flow through was used as the source of AHLs for the biosensor testing. As stated in the Supplementary Materials section (Protocol S2), the resulting biofilms were macroscopically visualized using CLSM and were 3D-processed by IMARIS 2.7 software (Bitplane AG, Zurich, Switzerland). 2.4.5. Quorum-Sensing Inhibition Assay The ability of the biosensor to detect quorum-sensing inhibitors was evaluated by incubating the bioreporter microbeads with various concentrations of a synthetic quorumsensing inhibitor (furanone-C-30) in the presence of a known concentration of autoinducers or cell-free supernatants of the wild P. aeruginosa strain (PAO1). The bioluminescent measurement was carried out as usual, and the percent inhibition was determined according to the following equation: Quorum-sensing inhibition (%) = Induction Factor in the presence o f Inhibitor Indcution Factor in the Absence o f Inhibitor ×100. 2.5. Development of Optical Biosensors As a proof of concept for biosensor development, the bioreporter hydrogels were adapted to the fiber optic setup established in our laboratory [ 21 – 24 ]. The set up comprises the biological recognition elements (LasR and RhlR bioreporter strains) attached to a transducer (fiber optic) whose far end is in contact with the detector (photomultiplier tube), as shown in Figure 3. 2.5.1. Instrument Setup The photon-counting system was designed and built in our laboratory. The instrument setup was placed in a light-tight box (Figure 3). A manual shutter (71430, Oriel) was placed in front of the detector to prevent damage to the photon-counting unit by environmental light [ 23 ]. The analog output signal was the mean of the AC components (pulses) generated after multi-anode amplification in the photomultiplier tube (PMT). The bioluminescence measurements at 490 nm were recorded using a Hamamatsu HC135-01 PMT Sensor Module, which combines the sensitivity of a photomultiplier tube with the advanced capabilities of a microcontroller [ 21 ]. The detector was optimized to the blue light region and includes a 21 mm-diameter active area convenient for gathering light radiation without any opticalfocusing elements [23]. 2.5.2. Experimental Probe Setup The optical fiber, with its adsorbed bioreporter, was placed in a 0.5 mL conical tube (Jonplast, Lesmo, MB, Italy) containing the test sample solution and was held at the epicenter of the light-proof sample holder to avoid ambient light interferences. The far end Biosensors 2024,14, 383 9 of 22 of the fiber was held by a fiber holder (FPH-DJ, Newport) and placed into an adjustable single-fiber mount (77837, Oriel). 2.5.3. Preparation of the Optical-Fiber Surface and Bioluminescent Measurements Multimode optical fibers, PUV 400 BN (CeramOptec, GmBH, Bonn, Germany), were used in these experiments. They are made of a pure silica core diameter of 400 µ m, with a refractive index of 1.4571 (at 633 nm) and a cladding diameter of 440 µ m with a refractive index of 1.4011 (at 633 nm). The black nylon jackets were stripped away from a 1 cm long optical-fiber tip, which was then used for the immobilization of bioreporter strains [ 21 , 22 ]. The 1 cm-optical-fiber tip was exposed to a bacterial alginate suspension and then dipped into a calcium chloride solution, entrapping the bioreporter within a hardened calcium alginate matrix. Repeating these steps six (6) times resulted in a thick adlayer, increasing the number of bioreporters immobilized on the optical-fiber transducer [ 22 ]. At this step, the functionalized optical fibers were ready for the experimental detection of autoinducers. Fibers were used immediately after preparation. 2.6. Statistical Analysis A two-way or one-way ANOVA, followed by a Tukey multiple comparisons test, was conducted using GraphPad Prism version 8.0.2 for Windows (GraphPad Software, San Diego, CA, USA, www.graphpad.com, accessed on 26 November 2023). 3. Results 3.1. Encapsulation and Optimization 3.1.1. Optimization of Microbead Formations Using the experimental setup described earlier, it was possible to control the bead’s size by controlling the microfluidic pump’s air pressure and flow rate. At a constant flow rate of 10 mL/h, the higher air pressure (valve opening) produced correspondingly smallersized beads until 40 psi (Figure S3). Much smaller beads were obtained when the flow rate was tripled (30 mL/h) at a constant air pressure of 40 psi, resulting in an average bead diameter of 956.23 ± 38 µ m. Therefore, a 30 mL/h flow rate, 40 psi pressure, and 15-unit aperture were adopted in all the subsequent experiments. 3.1.2. Effect of Encapsulation Methods Next, our research investigated the encapsulation of the bacteria in the microbeads. The process of encapsulating bacteria within alginate presents challenges due to potential gel degradation from ion exchange or rapid bacteria growth, which could lead to bacteria escaping from the gel matrix [ 30 ]. To address this issue, the polymer shell surrounding the alginate core can serve to protect the cells and maintain them within the core. It is important to note that the polymer shell allowed for the diffusion of small molecules in and out of the core, which is crucial for maintaining cell viability. Two approaches were investigated to prepare a poly-lysine-reinforced alginate microbead encapsulation system. The first approach involved preparing an alginate inner sphere followed by stepwise incubation in a poly-lysine solution to create a thin membrane around the alginate (Figure 2). In the second approach, the microbeads were dropped into a calcium-poly-lysine solution, followed by two additional incubations in a PLL solution (Figure S4). Both approaches led to reinforced alginate microbeads that are stable when incubated in the presence of 5% sodium citrate. 3.1.3. ATR-FTIR Data Figure 4compares the ATR-FTIR absorbance spectrum of the outer portion of lyophilized alginate-PLL and calcium-alginate microbeads. The absorbance bands that were characteristic of the alginate were identified with a broad peak around 3304 cm −1 , which were ascribed to the stretching vibrations of the hydrogen-bonded OH groups and the two peaks around 1590 and 1416 cm −1 representing asymmetric and symmetric stretching vibrations, Biosensors 2024,14, 383 16 of 22 detect P. aeruginosa-specific autoinducers in the presence of other bacteria in the clinical and environmental samples. Biosensors 2024, 14, x FOR PEER REVIEW 16 of 23 panel shows the dose–response relationships inferred from the data in the left panel. Linear regression lines (bold) were drawn on logarithmic and linear (upper insert) scales, and the 95% confidence interval limits were the black dotted lines parallel to the regression lines. The equation and the R2 value for each regression line are shown. Data are the mean ± SD of three independent experiments. Figure 13. The bioreporter specificity test. The RhlR (A) and LasR (B) response toward 5 µM each of synthetic QS molecules, respectively. The second experiment was conducted with either 20 µL of cell-free supernatants of each Gram-negative bacterium such as Escherichia coli (E. coli), Pseudomonas aeruginosa (PAO1), and Acinetobacter baumannii (A. baumannii) (72 h biofilm set up), or 5 µM of C4-HSL for the RhlR strain (C) or 5 µM of 3-oxo-C12-HSL for the LasR strain (D). 3.3. Selectivity and Inhibition Studies 3.3.1. Selectivity of the Bioreporters to the Synthetic and Secreted QS Molecules The bioluminescent response of the bioreporter was tested in the presence of synthetic autoinducers (Figure 13A,B) and cell-free supernatants obtained from Pseudomonas aeruginosa, Acinetobacter baumannii, and Escherichia coli (Figure 13C,D). Each strain of the reporter bacteria in this study distinctly responded to their cognate autoinducers. The bioreporter selectively responded to the cell-free samples of P. aeruginosa but not E. coli or A. baumannii, as shown in Figure 14. This is desirable because the biosensor can selectively detect P. aeruginosa-specific autoinducers in the presence of other bacteria in the clinical and environmental samples. C4-HSL C6-HSL C6-oxo-HSL C8-oxo-HSL C12-oxo-HSL 0 20 40 60 80 100 PDL RhlR 16.11.23 A 3-oxo-C12-HSL C4-HSL C6-HSL 3-oxo-C6-HSL 3-oxo-C8-HSL 0 20 40 60 80 py Induction Factor B C4-HSL (5 M) PAO1 A. baumannii E. coli 0 50 100 C 3-oxo-C12-HSL PAO1 A. baumannii E. coli 0 20 40 60 80 100 Induction Factor D Induction Factor Induction Factor AB Figure 14. The QS inhibitory effect of furanone C-30 in the presence of synthetic AHLs. (A) The inhibition of RhlR signaling in the presence of 5 µ M of C 4 -HSL and (B) the inhibition of the LasR bioluminescence system in the presence of 5 µ M of 3-OC 12 -HSL. Luminescent readings were taken for 10 h, and the maximum relative light unit (RLU) was plotted. The results are the mean ± SD of biological triplicates. 3.3.2. Determination of AIs in the Flow-Cell Biofilm Effluent Biofilm formation was followed microscopically and as a function of autoinducer secretion, and it was confirmed by luminescence assays with RlhR and LasR bioreporters (Figures S13 and S14). The effluents of flow-cell biofilm were collected at 12, 24, 48, and 72 h intervals and filtered. The filtered effluents were used as the source of autoinducers, and the bioluminescence responses were recorded as usual. The bioluminescence was interpolated from the standard plot. 3.3.3. Inhibition Studies The biosensor’s ability to detect the presence of an inhibitor was tested by incubating it with either synthetic autoinducers (Figure 14) or cell-free supernatants of the wild PAO1 type in the presence of varying concentrations of QSI (Figures S15 and S16). 3.4. Storability Assessments The bioreporter beads were tested for long storability under different temperatures. First, the bacterial beads were stored at − 80 ◦ C for 48 h, after which their activities were tested and compared with the corresponding beads stored at 4 ◦ C (Figure S11). There was no statistically significant difference between the responses to the added autoinducers. The bioreporters were further stored at 4 ◦ C for at least 57 days, and the activities were tested at intervals (Figure 15A,B). After that, the beads were stored at − 80 ◦ C and 4 ◦ C for 30 days, after which the activities were tested and compared as usual and found to be the same at p< 0.05 (Figure 15C,D). Also, an attempt was made to store the beads at room temperature in dry and wet conditions. As shown in Figure 16, the beads stored in a dried state for 24 h and 5 days retained the sensing ability to the autoinducer without statistically significant differences (Figure 16). However, in both RhlR and LasR strains, when the beads were stored in sterile DDW for 24 h at room temperature, there was a significant (p< 0.05) loss in the sensing ability of the immobilized bioreporters. Similarly, when the beads were lyophilized, there was a considerable loss in the residual bioluminescent response (Figure S17). Biosensors 2024,14, 383 17 of 22 Biosensors 2024, 14, x FOR PEER REVIEW 17 of 23 Figure 14. The QS inhibitory effect of furanone C-30 in the presence of synthetic AHLs. (A) The inhibition of RhlR signaling in the presence of 5 µM of C4-HSL and (B) the inhibition of the LasR bioluminescence system in the presence of 5 µM of 3-OC12-HSL. Luminescent readings were taken for 10 h, and the maximum relative light unit (RLU) was plotted. The results are the mean ± SD of biological triplicates. 3.3.2. Determination of AIs in the Flow-Cell Biofilm Effluent Biofilm formation was followed microscopically and as a function of autoinducer secretion, and it was confirmed by luminescence assays with RlhR and LasR bioreporters (Figures S13 and S14). The effluents of flow-cell biofilm were collected at 12, 24, 48, and 72 h intervals and filtered. The filtered effluents were used as the source of autoinducers, and the bioluminescence responses were recorded as usual. The bioluminescence was interpolated from the standard plot. 3.3.3. Inhibition Studies The biosensor’s ability to detect the presence of an inhibitor was tested by incubating it with either synthetic autoinducers (Figure 14) or cell-free supernatants of the wild PAO1 type in the presence of varying concentrations of QSI (Figures S15 and S16). 3.4. Storability Assessments The bioreporter beads were tested for long storability under different temperatures. First, the bacterial beads were stored at −80 °C for 48 h, after which their activities were tested and compared with the corresponding beads stored at 4 °C (Figure S11). There was no statistically significant difference between the responses to the added autoinducers. The bioreporters were further stored at 4 °C for at least 57 days, and the activities were tested at intervals (Figure 15A,B). After that, the beads were stored at −80 °C and 4 °C for 30 days, after which the activities were tested and compared as usual and found to be the same at p < 0.05 (Figure 15C,D). Also, an attempt was made to store the beads at room temperature in dry and wet conditions. As shown in Figure 16, the beads stored in a dried state for 24 h and 5 days retained the sensing ability to the autoinducer without statistically significant differences (Figure 16). However, in both RhlR and LasR strains, when the beads were stored in sterile DDW for 24 h at room temperature, there was a significant (p < 0.05) loss in the sensing ability of the immobilized bioreporters. Similarly, when the beads were lyophilized, there was a considerable loss in the residual bioluminescent response (Figure S17). ✱✱ Figure 15. Storability of the bioreporter beads in refrigerator conditions. (A) RhlR and (B) LasR 2 µ M inducers. The RhlR strain experienced a significant (shown as ** at p< 0.05) reduction in activities between Days 7 and 50 and remained fairly constant thereafter. Comparison of the effect of storage at 4 and − 80 degrees (for 30 days) on the luminescence. The residual activity of RhlR (C) and LasR (D) was compared after storage in the refrigerator and freezer at − 80 ◦ C for a minimum of 30 days. Two concentrations of each AHL were tested, and the results are reported as the mean ± SD, n = 4. Statistical analysis revealed no significant differences in the readings at p< 0.05. Biosensors 2024, 14, x FOR PEER REVIEW 18 of 23 Figure 15. Storability of the bioreporter beads in refrigerator conditions. (A) RhlR and (B) LasR 2 µM inducers. The RhlR strain experienced a significant (shown as ** at p < 0.05) reduction in activities between Days 7 and 50 and remained fairly constant thereafter. Comparison of the effect of storage at 4 and −80 degrees (for 30 days) on the luminescence. The residual activity of RhlR (C) and LasR (D) was compared after storage in the refrigerator and freezer at −80 °C for a minimum of 30 days. Two concentrations of each AHL were tested, and the results are reported as the mean ± SD, n = 4. Statistical analysis revealed no significant differences in the readings at p < 0.05. Figure 16. Storage at room temperature. (A) RhlR and (B) LasR results. The results are presented as the mean ± SD, (n = 4). RT: beads stored dried at room temperature. 4. Discussion Monitoring quorum-sensing molecules (QSMs) has become a crucial analytical tool in researching bacteria-related disturbances. The levels of these signaling molecules can potentially serve as biomarkers for disease and environmental monitoring. Therefore, there is an increasing demand for analytical methods that can sensitively, quantitatively, rapidly, and cost-effectively detect bacterial QSMs. Whole-cell biosensing systems have been shown to detect QSMs directly with little or no sample pretreatment. Moreover, microorganisms are tolerant of suboptimal assay conditions and can be prepared in unlimited quantities relatively inexpensively, making them candidates of choice for highthroughput screening, miniaturization, and automation [38]. In this study, we postulated that the stable immobilization of bioreporter bacteria within reinforced alginate could offer robust whole-cell sensing systems for monitoring the levels of AHLs. First, poly-lysine-coated alginate beads were prepared, which remained intact in the presence of the known Ca2+ chelator citrate (Figure 7). The bacteria were retained within the beads, and the bioluminescent assays demonstrated the passage of autoinducers and quorum-sensing inhibitors into the core of the beads. The alginate-poly-lysine capsules utilized in this research exhibited similar physical properties regardless of the type of poly-lysine (L and D) isomers and identical permeability to the QS molecules. This suggests that either Lor D-isomers of poly-lysine can be employed to reinforce alginate based on specific applications. For instance, the D-isomer is known to be less susceptible to proteolysis, making it suitable for the encapsulation of applications that are likely to encounter protease. For this study, the L-isomer of polylysine was utilized in all experiments. The physicochemical analyses presented clear evidence of the presence of alginate and PLL or PDL in the membrane of the APA microcapsules. The X-ray photoelectron spectroscopy (XPS) analysis of the microcapsules’ elemental composition indicated that relatively high amounts of PLL were very close to the surface, within the outermost being 10 nm. Tam et al. [31] and De Vos et al. [33,39] obtained similar surface compositions using XPS, implying that these results are predictable. Furthermore, the spectroscopic analyses indicated that both alginate and the PLL (or PDL) were present at the surface, suggesting that at least some of the polysaccharides bound to the PLL membrane during the capsule’s final incubation are diluted alginate. We excluded the possibility that the alginate at the surface originated from the gel core since XPS detected no calcium. Thu et al. also demonstrated that, using radiolabeling techniques, the coating alginate binds to the microcapsule’s PLL layer. Furthermore, the confocal 5 days RT 24 hrs RT 24 hrs DDW 4 C (5 days) Induction Factor 5 days RT 24 hrs RT 24 hrs DDW 4 C (5 days) Induction Factor AB Figure 16. Storage at room temperature. (A) RhlR and (B) LasR results. The results are presented as the mean ±SD, (n = 4). RT: beads stored dried at room temperature. 4. Discussion Monitoring quorum-sensing molecules (QSMs) has become a crucial analytical tool in researching bacteria-related disturbances. The levels of these signaling molecules can potentially serve as biomarkers for disease and environmental monitoring. Therefore, there is an increasing demand for analytical methods that can sensitively, quantitatively, rapidly, and cost-effectively detect bacterial QSMs. Whole-cell biosensing systems have been shown to detect QSMs directly with little or no sample pretreatment. Moreover, microorganisms are tolerant of suboptimal assay conditions and can be prepared in unlimited quantities relatively inexpensively, making them candidates of choice for high-throughput screening, miniaturization, and automation [38]. In this study, we postulated that the stable immobilization of bioreporter bacteria within reinforced alginate could offer robust whole-cell sensing systems for monitoring the levels of AHLs. First, poly-lysine-coated alginate beads were prepared, which remained intact in the presence of the known Ca 2+ chelator citrate (Figure 7). The bacteria were retained within the beads, and the bioluminescent assays demonstrated the passage of autoinducers and quorum-sensing inhibitors into the core of the beads. Biosensors 2024,14, 383 18 of 22 The alginate-poly-lysine capsules utilized in this research exhibited similar physical properties regardless of the type of poly-lysine (L and D) isomers and identical permeability to the QS molecules. This suggests that either Lor D-isomers of poly-lysine can be employed to reinforce alginate based on specific applications. For instance, the D-isomer is known to be less susceptible to proteolysis, making it suitable for the encapsulation of applications that are likely to encounter protease. For this study, the L-isomer of poly-lysine was utilized in all experiments. The physicochemical analyses presented clear evidence of the presence of alginate and PLL or PDL in the membrane of the APA microcapsules. The Xray photoelectron spectroscopy (XPS) analysis of the microcapsules’ elemental composition indicated that relatively high amounts of PLL were very close to the surface, within the outermost being 10 nm. Tam et al. [ 31 ] and De Vos et al. [ 33 , 39 ] obtained similar surface compositions using XPS, implying that these results are predictable. Furthermore, the spectroscopic analyses indicated that both alginate and the PLL (or PDL) were present at the surface, suggesting that at least some of the polysaccharides bound to the PLL membrane during the capsule’s final incubation are diluted alginate. We excluded the possibility that the alginate at the surface originated from the gel core since XPS detected no calcium. Thu et al. also demonstrated that, using radiolabeling techniques, the coating alginate binds to the microcapsule’s PLL layer. Furthermore, the confocal microscopic study of the binding of PLL to the alginate revealed the formation of a polyanion/polycation outer, thin-shell-like, and rough membrane (Figure 6) similar to what was observed elsewhere [ 19 , 32 ]. The threedimensional image of alginate-PLL capsules showed no visible holes in the PLL membranes, indicating that PLL covers the capsule completely. It was further demonstrated that the PLL–alginate interaction was unchanged when stored for two weeks and two years [ 19 ]. This shows the stability of the additional support provided by the PLL. It was observed that, in this study, the PLL-coated alginate beads were protected from the effects of a known metal chelator, sodium citrate. Chelators such as EDTA and sodium citrate can induce the complete degradation of alginate gels, while the gels may undergo partial degradation when immersed in buffers like phosphate-buffered saline (PBS). This occurs as some Ca 2+ cross-links are exchanged with Na + , reducing mechanical rigidity and cross-link density and ultimately weakening the gel. When the coated and uncoated alginate beads were incubated in citrate (2.5–5% w/v), the uncoated alginate capsule was completely dissolved. In contrast, PLL-coated alginate beads, as well as the dual layer having the interpenetrating network, persisted in a 5% citrate solution for over 14 h at room temperature (Figures 7and S4). This shows the protective effect of the PLL layers on the materials encapsulated within the alginate beads. Thus, regardless of any loss of cross-links from the alginate core, the poly-lysine layers will ensure that the microcapsules preserve their structural integrity, similar to the report of Gugerli et al. [ 28 ]. Additionally, the dried beads regained their sphericity upon rehydration. Pseudomonas aeruginosa contains two transcription regulators (LasR and RhlR) that, when complexed with their specific autoinducers (3-oxo-C 12 -HSl and C 4 -HSL, respectively), activate the transcription of different virulence-associated traits and enzymes involved in rhamnolipid biosynthesis [ 40 ]. In this study, mutant strains of Pseudomonas aeruginosa were utilized. These strains have a knockout on the gene expressing 3-oxo-C 12 -HSL and C 4 -HSL autoinducers, necessitating the exogenous addition of the autoinducer for the activation of the transcription of the promoter-controlled bacterial genes fused to the reporter (luciferase) gene (Figure S1). Consequently, the expression of the reporter genes in the mutant bioreporter can be correlated with the amount of exogenously added autoinducers in the media, forming the basis for detecting QS molecules (autoinducers) using bioreporter bacterial strains. Two mutant strains of P. aeruginosa (LasR and RhlR) were genetically engineered to express bioluminescence in response to specific quorum-sensing (QS) molecules. These strains were individually encapsulated within alginate-PLL beads following the procedure shown in Figure 2. LasR and RhlR can quantitatively respond to 3-oxo-C12-HSL and C 4 -HSL, respectively. Increased bioluminescence intensity was observed with increasing Biosensors 2024,14, 383 19 of 22 concentrations of the AHL compounds used. Upon preparation, the bioreporter-loaded microcapsules were incubated in a culture media overnight (20 h) at 30 ◦ C and 150 rpm. Subsequently, viability testing was performed, and confocal imaging confirmed the viability of the encapsulated bioreporter. The bioreporter-loaded beads were subjected to various concentrations (including the absence) of autoinducers, and the resulting bioluminescence response was recorded. The obtained results provide evidence that the bacteria remain viable and exhibit their genetically programmed response, thus retaining the sensing functionality. The plasmids used in this study were constructed by taking advantage of the RhlI/RhlR and LasI/LasR quorum-sensing systems of the Gram-negative bacterium P. aeruginosa. C 4 - HSL and 3-oxo-C 12 -HSL are known to be the respective cognate activator molecules, i.e., the natural ligands that P. aeruginosa synthesize, sense, and respond to [ 41 ]. In this study, we observed increased bioluminescence intensity with increasing concentrations of C 4 -HSL and 3-oxo-C 12 -HSL. The RhlR-based bioreporter beads demonstrated a dynamic range and limit of detection of 10 µ M–0.1 nM and 50 pM, respectively, for C 4 -HSL. In contrast, the LasR-based bioreporter showed a dynamic range and a detection limit of 5 µ M–0.2 nM and 0.1 nM, respectively, for 3-oxo-C 12 -HSL. A calibration curve was generated with either C 4 -HSL or 3-oxo-C 12 -HSL at known concentrations, which can be used to calculate the concentration of C 4 -HSL or 3oxo-C 12 -HSL activity in each sample. The molecule C 4 -HSL, at a concentration of 10 µ M, induced the maximum response in the RhlR system carrying the plasmid pKD-rhlA. Meanwhile, 2 µ M of the 3-oxo-C 12 -HSL molecule exhibited a maximum response in the bioreporter containing the plasmid pKD201 lasl. These findings align with previous studies, indicating that long-chain AHLs induce the LasR regulatory system to a greater extent, whereas short-chain AHLs efficiently activate the RhlR regulatory system [ 42 ]. Additionally, the limit of detection (LOD) was defined as the lowest concentration of AHL capable of inducing a 20% increase in luminescence by the biosensor without any added molecule. The wide range of response and the high sensitivity of these bioreporters suggest that the bacteria strain in this study could be used for the direct determination of the 3-oxo-C 12 -HSL and C4-HSL levels in P. aeruginosa laboratory cultures and clinical samples where P. aeruginosa is implicated. According to previous reports, the highest concentration of AHLs in laboratory cultures and clinical samples (cystic fibrosis sputa) is ≤ 28 µ M and ≤21 nM, respectively [8,43]. After assay optimization, our whole-cell sensing systems were employed to detect the presence of the AHLs in samples collected from cell-free biofilm and planktonic bacteria cultures. We also evaluated our whole-cell sensing systems for their ability to detect the presence of quorum-sensing inhibitors (QSI) by measuring the residual QS activity in the presence of autoinducers and QSI. To this end, we tested our system’s response to furanone C-30 (FC30), a well-known QSI molecule that interferes with RhlR and LasR QS signaling [ 44 ]. The system exhibited a similar inhibition response to FC30 when tested in the presence of either synthetic autoinducers (Figure 14) or a cell-free culture of the PAO1 strain of P. aeruginosa (Figure S15). Furthermore, the system maintained its sensing ability when stored at 4 ◦ C for 60 days (Figure 15) and when stored for over 85 days) and frozen at −80 ◦C . Lyophilization resulted in a significant loss of bioluminescence response, but simple air drying of the bioreporter did not affect its sensing capability. The biosensor strains did not require special treatment to revive them after long storage; they existed as ready-to-deploy microcapsules. Overall, the bioreporter encapsulated in alginatePLL microbeads has the potential to be a sensitive, easy-to-prepare, and easy-to-deploy analytical unit suitable for various biotechnological applications in both developed and low-income countries. Biosensors 2024,14, 383 20 of 22 5. Conclusions We developed hydrogel-immobilized bioreporters as on-demand, whole-cell biosensing systems for the sensitive, selective, and rapid detection of AHLs in spiked samples and laboratory cultures. The method is simple, sensitive, reproducible, and amenable for point-of-need deployment without extensive sample preparation. The RhlR-based bioreporter beads demonstrate a dynamic range and limit of detection of 10 µ M–0.1 nM and 50 pM, respectively, for C 4 -HSL, while the LasR-based bioreporter showed a dynamic range and a limit of detection of 5 µM–0.2 M and 0.1 nM, respectively, for 3-oxo-C12-HSL. The immobilized bioreporters can be stored stably and refrigerated as ready-to-use microbeads for over 60 days. Furthermore, the developed method is cost-effective, robust, and adaptable for screening quorum-sensing (QS) inhibitors. We believe this novel biosensing system could be utilized for diagnosis and environmental monitoring. Additionally, our systems could serve as a valuable tool for screening novel QSI-based drugs active against Pseudomonas aeruginosa infection and a variety of diseases that involve QS in their pathogenesis. Supplementary Materials: The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/bios14080383/s1. Figure S1: The plasmids used in this study; Figure S2: The structures of QS molecules used in this study; Figure S3: Bead sizes are a function of air pressure; Figure S4: Alginate-PLL IPN showed enhanced stability chelators; Figure S5: The ATRFTIR spectra of calcium alginate, sodium alginate, and calcium alginate-poly-lysine; Figure S6: XPS atomic peak composition of the lyophilized alginate-poly-lysine beads; Figure S7: The micrograph of live/dead, stained bioreporter beads; Figure S8: Bioluminescent measurement normalized by cell density; Figure S9: The dose-dependent response of the LasR bioreporter; Figure S10: Different layers of the microbead performances; Figure S11: Storage for 48 h in a refrigerator and freezer; Figure S12: Flow-cel l biofilm experiment; Protocol S1: The detection of AHLs in the biofilm effluent obtained in the flow-cell experiment; Protocol S2: Biofilm visualization; Figure S13: Typica l visualization of the biofilm after 24, 48, and 72 h; Figure S14: The bioluminescent response of the bioreporters to biofilm effluents; Figure S15: Inhibitory effect of furanone C-30 (FC30) on the activity of RhlR; Figure S16: At higher concentrations of F-C30, the bioluminescence of the RhlR reporter was almost quenched; Figure S17: Activity of the lyophilized LasR beads; Figure S18: The intermachine differences; and Figure S19: Optical-fiber, whole-cell biosensors. Reference [ 29 ] is cited in the supplementary materials. Author Contributions: Conceptualization, A.A.P. and R.S.M.; methodology, A.A.P. and Y.S.K.; validation, R.S.M. and A.K.; formal analysis, A.A.P.; investigation, A.A.P. and Y.S.K.; writing—original draft preparation, A.A.P.; writing—review and editing, A.A.P., Y.S.K., A.K. and R.S.M.; supervision, R.S.M. and A.K.; project administration, R.S.M. and A.K. All authors have read and agreed to the published version of the manuscript. Funding: This research was funded by the European Union Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement no. 872662, IPANEMA project. Institutional Review Board Statement: Not applicable. Informed Consent Statement: Not applicable. 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