Supplementary material 3 from: Esmaeilzadeh-Salestani K, Queiroz MB, Mikryukov V, Uszok S, Goto BT, Tedersoo L, Magurno F (2025) Morphological and phylogenetic analysis of the early-diverging lineage of Glomeromycota suggest two new genera and recombinations in Archaeosporales. MycoKeys 124: 249-273. https://doi.org/10.3897/mycokeys.124.166449
Abstract
ITS and LSU barcodes selected for the lineages identified in the study for each genus in Archaeosporaceae
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Figure S1. Separation of Antiquispora sp.1 from other species of Antiquispora based on ITS region (ITS2 positions 146–165 tatttttcgcaactttttgg; no mismatch allowed) and LSU (positions 676–702 ctccgagtcctcaacgggatagagcaa; one mismatch allowed). Figure S2. Separation of Antiquispora sp.2 from other species of Antiquispora based on the ITS region (ITS2 positions 188–207 tgaggaaaagcgcatacaat; one mismatch allowed) and LSU (positions 445–464 aatcaagcgaagcatttgtc; no mismatch allowed). Figure S3. Separation of Antiquispora sp.3 from other species of Antiquispora based on the ITS region (ITS2 positions 10–29 ggtgggaatactctttgggg; no mismatch allowed) and LSU (positions 447– 466 aatcaagcgaatttagctgt; no mismatch allowed).
Figure S4. Separation of Antiquispora sp.4 from other species of Antiquispora based on the ITS region (ITS2 positions 189–211 tggagaaaaggcacatacaaatt; no mismatch allowed) and LSU (positions 663–683 tgctgatggtcatttggaaat; no mismatch allowed). Figure S5. Separation of Antiquispora sp.5 from other species of Antiquispora based on the ITS region (ITS2 positions 183–204 tttctctttgtcgagaaggggc; one mismatch allowed) and LSU (positions 222–243 ccttgccgagttgtgtttgcag; one mismatch allowed). Figure S6. Separation of Antiquispora sp.6 from other species of Antiquispora based on the ITS region (ITS2 positions 204–223 acatacaaggtattttttat; no mismatch allowed) and LSU (positions 625– 649 catacctgttctctgaacggaagcc; no mismatch allowed).
Figure S7. Separation of Antiquispora sp.7 from other species of Antiquispora based on the ITS region (ITS1 positions 40–61 cacaatccaagtttgtaataat; one mismatch allowed) and LSU (positions 236– 255 gtcgtgttcgccatgtgttg; no mismatch allowed). Figure S8. Separation of Antiquispora sp.8 from other species of Antiquispora based on the ITS region (ITS2 positions 139–158 gcaactcttgagctgaattc; one mismatch allowed) and LSU (positions 453472 cgaattctgacatccattgg; no mismatch allowed). Figure S9. Separation of Antiquispora sp.9 from other species of Antiquispora based on the ITS region (ITS2 positions 201–220 ctctgggagaaaaaggcgca; no mismatch allowed) and LSU (positions 151–170 tgcattccgcagtacacctt; no mismatch allowed).
Figure S10. Separation of Antiquispora sp.10 from other species of Antiquispora based on the ITS region (ITS2 positions 43–62 agtatttcgcgatatacttt; no mismatch allowed) and LSU (positions 639–658 ctgaaagacttgcaagtaga; one mismatch allowed). Figure S11. Separation of Antiquispora sp.11 from other species of Antiquispora based on the ITS region (ITS2 positions 197–218 aaaagagaaaaggcacttgcag; one mismatch allowed) and LSU (positions 632–654 gcctgttctctgaaaggcttacg; one mismatch allowed). Figure S12. Separation of Antiquispora sp.12 from other species of Antiquispora based on the ITS region (ITS2 positions 9–28 tcgaaagcgaataatatgta; no mismatch allowed) and LSU (positions 459–478 agcggttccttgtgttcagc; one mismatch allowed).
Figure S13. Separation of Antiquispora sp.13 from other species of Antiquispora based on ITS region (ITS1 positions 4–18 gaatgcgagagttgt; no mismatch allowed) and LSU (positions 459–478 agtggttccatgtgagcagc; no mismatch allowed). Figure S14. Separation of Antiquispora sp.14 from other species of Antiquispora based on the LSU (positions 220–239 cattgccgagtttgcgtgcg; one mismatch allowed). Figure S15. Separation of Antiquispora sp.15 from other species of Antiquispora based on the ITS region (ITS2 positions 65–84 aatatttattttgattgctc; no mismatch allowed) and LSU (positions 223–242 attgtcgagatcgtgtcgct; no mismatch allowed).
Figure S16. Separation of Antiquispora sp.16 from other species of Antiquispora based on the SSU region (positions 1680–1699 catctaattgctagcaatag; one mismatch allowed) and LSU (positions 621– 640 cttgtctattctctgaatag; no mismatch allowed). Figure S17. Separation of Antiquispora sp.17 from other species of Antiquispora based on the ITS region (ITS2 positions 19–38 aaactgtatcggaatattga; no mismatch allowed) and LSU (positions 619– 638 gtacttacctgccctctgaa; no mismatch allowed). Figure S18. Separation of Antiquispora sp.18 from other species of Antiquispora based on the ITS region (ITS2 positions 25–44 ctctaggtagagacagcagt; one mismatch allowed) and LSU (positions 644– 663 agtacatttagacaacagca; no mismatch allowed).
Figure S19. Separation of Antiquispora sp.19 from other species of Antiquispora based on the ITS region (ITS2 positions 56–75 taacgcgtactgtgtgttac; no mismatch allowed) and LSU (positions 144–163 gtagagacgtactccgcgat; no mismatch allowed). Figure S20. Separation of Antiquispora sp.20 from other species of Antiquispora based on the ITS region (ITS2 positions 164–183 acaatgcttgttgggacttt; no mismatch allowed) and LSU (positions 224– 244 ccatgccattgttgtgtttgc; no mismatch allowed). Figure S21. Separation of Antiquispora sp.21 from other species of Antiquispora based on the ITS region (ITS2 positions 26–45 aaagtattgtgtattggagt; one mismatch allowed) and LSU (positions 155– 174 taccgcgtagcaagttggcc; no mismatch allowed).
Figure S22. Separation of Andinospora sp.1 from other species based on the ITS region (ITS2 positions 160–179 ccaacttgtttgattctttt; one mismatch allowed) and LSU (positions 227–246 atcgtgattcagatgtcttg; no mismatch allowed). Figure S23. Separation of Andinospora sp.2 from other species based on the ITS region (ITS2 positions 139–158 taccatcaagtcaatttcta; one mismatch allowed) and LSU (positions 588–609 ggatcgaggttgaggtttgcga; no mismatch allowed). Figure S24. Separation of Andinospora ecuadoriana from other species based on the ITS region (ITS2 positions 62–81 ttgtataggtgatggtgatt; no mismatch allowed) and LSU (positions 470–489 gataaaattatttgatgcac; no mismatch allowed).
Figure S25. Separation of Palaeospora spainii from other species based on the ITS region (ITS2 positions 113–132 tatgtacattgtataatctt; no mismatch allowed) and LSU (positions 225–244 ctgtcgtgatcttgatatcg; no mismatch allowed). Figure S26. Separation of Archaeospora sp.1 from other species based on the ITS region (ITS2 positions 3–22 aaaattaatcttggctaaaa; no mismatch allowed) and LSU (positions 164–183 tcttgatccgacgaaaagtc; no mismatch allowed). Figure S27. Separation of Archaeospora sp.2 from other species based on the ITS region (ITS2 positions 50–65 ttacctcttctgtggt; one mismatch allowed).