New and poorly known Pleurota Hübner, [1825] species from peninsular Spain, the Balearic Islands, and the Canary Islands (Lepidoptera: Oecophoridae, Pleurotinae)

43 SHILAP Revista de lepidopterología, 53 (209) marzo 2025: 43-74 https://doi.org/10.57065/shilap.1024 eISSN: 2340-4078 ISSN: 0300-5267 https://shilap.org 1 New and poorly known Pleurota Hübner, [1825] species from peninsular Spain, the Balearic Islands, and the Canary Islands (Lepidoptera: Oecophoridae, Pleurotinae) Jukka Tabell, Martin Honey, Théo Leger,Marko Mutanen, Timo Nupponen & Pasi Sihvonen Abstract Four new Pleurota Hübner, [1825] species from peninsular Spain, one species from the Balearic Islands and one species from the Canary Islands are described: P. rondaensis Tabell & Nupponen, sp. nov., P. pyrenaella Tabell, sp. nov., P. balearicaTabell & Honey, sp. nov., P. malagaensis Tabell & Nupponen, sp. nov., P. grancanariella Tabell, sp. nov. and P. subnobilella Tabell, sp. nov. In addition, adults, and genitalia of seven poorly known Spanish Pleurota species are illustrated, most of them for the first time, and photos of several type specimens are presented. Lectotypes for P. protasella Staudinger, 1883, P. glitzella (Staudinger, 1883), P. pleurotella (Staudinger, 1871), P. sobriella(Staudinger, 1859) and P. nobilellaRebel, 1901 are designated. DNA barcodes of the aforementioned species are compared with those of all other Pleurotinae available to us in the BOLD database. Each of the species studied herein has a unique BIN (Barcode Index Number). Keywords: Lepidoptera, Oecophoridae, Pleurotinae, Pleurota, new species, Spain. Especies nuevas y poco conocidas de Pleurota Hübner, [1825] de España peninsular, Islas Baleares e IslasCanarias (Lepidoptera: Oecophoridae, Pleurotinae) Resumen Se describen cuatro nuevas especies de Pleurota Hübner, [1825] de España peninsular, una especie de las Islas Baleares y una especie de las Islas Canarias: P. rondaensis Tabell & Nupponen, sp. nov., P. pyrenaella Tabell, sp. nov., P. balearica Tabell & Honey, sp. nov., P. malagaensis Tabell & Nupponen, sp. nov., P. grancanariella Tabell, sp. nov. y P. subnobilella Tabell, sp. nov. Además, se ilustran los adultos y la genitalia de siete especies españolas de Pleurota poco conocidas, la mayoría de ellas por primera vez y se presentan fotos de varios especímenes tipo. Se designan los lectotipos de P. protasella Staudinger, 1883, P. glitzella (Staudinger, 1883), P. pleurotella (Staudinger, 1871), P. sobriella (Staudinger, 1859) y P. nobilella Rebel, 1901. Los códigos de barras de ADN de las especies mencionadas se comparan con los de todos los demás Pleurotinae de que disponemos en la base de datos BOLD. Cada una de las especies aquí estudiadas tiene un BIN (Barcode Index Number) único. Palabras clave: Lepidoptera, Oecophoridae, Pleurotinae, Pleurota, nuevas especies, España. Introduction This article is the fourth contribution in a series of revisionary studies on the Palearctic Pleurotinae (Oecophoridae) (see Kaila et al. 2019, Tabell et al. 2019, 2021). The aim of this study is to present poorly known Spanish Pleurota Hübner, [1825] species and provide photos on both type and eISSN: 2340-4078 ISSN: 0300-5267 SHILAP Revista de lepidopterología, 53 (209) marzo 2025: 32 https://doi.org/10.57065/shilap.1024 https://shilap.org 44 SHILAP Revta. lepid., 53 (209) marzo 2025 recently collected specimens, as well as to describe new species. In the Spanish mainland the Pleurotinae fauna is diverse with 26 known taxa (Vives Moreno, 2014), and a remarkable portion of them are considered endemic to the Iberian Peninsula. Recent collecting excursions to Spain, carried out by Timo and Kari Nupponen and Jukka Tabell, have produced many Pleurota specimens, and some of the research findings are presented below. More results will be released in forthcoming articles. When checking the Pleurotinae specimens collected by Paul Grotenfelt in the early 1980s and 1990s, currently housed in MZH in University of Helsinki (Finland), the first author detected a series of Pleurota originating from the Canary Islands: Gran Canaria, Maspalomas. The examination of the genitalia indicated that the species may be new for science. Two samples were sent for DNA-studies, but both of them failed to yield DNA sequences. In order to get fresh material for genetic study, an excursion to Maspalomas and surrounding slopes was conducted by J. Tabell in 2019. The excursion was successful in the sense that aforementioned Pleurota species was found again. Simultaneously Per Falck, during his field work also succeeded in finding several specimens from two different areas. The species is described below as new to science. Previously only one member of the family Oecophoridae, namely Epicallima mikkolai (Lvovsky, 1995) was known to inhabit the Canary Islands (Falck et al. 2019), and therefore we report genus Pleurota here for the first time from the Canary Islands. When visiting the NHMUK in London in 2018, P. Sihvonen received a box with a series of small Pleurota species for study, collected by Martin Honey from three Balearic Islands. Small size and wing patterns indicated these may represent an unknown species, and this was confirmed later by morphological and molecular evidence. The genus Pleurota is reported here for the first time from the Balearic Islands. Material and methods Abbreviations MFNB = Museum für Naturkunde Berlin, Germany MNCN = Museo Nacional de Ciencias Naturales, Madrid, Spain MNHN = National Museum of Natural History, Paris, France MZH = Finnish Museum of Natural History, Helsinki, Finland NHMUK = Natural History Museum, London, United Kingdom HON = research collection of Martin Honey, London, United Kingdom JUJ = research collection of Jari Junnilainen, Vantaa, Finland NUP = research collection of Timo & Kari Nupponen, Espoo, Finland TAB = research collection of Jukka Tabell, Hartola, Finland bp = base-pair The holotype specimens and a part of the paratype specimens of newly described species are deposited in the public collections of MZH and NHMUK, and private collections of JUJ and NUP. Furthermore, a part of the paratype specimens is deposited in the private collection of TAB. The holotypes that are deposited in MZH have been digitized and have a unique graphical QR code and web identifier, following the open access policy of that museum. URL links for these holotype records are provided in the article. Tissues (legs) of at least of two samples of each species, when available, were sent to the Canadian Centre for DNA Barcoding (CCDB) to obtain molecular data of the 658 bp fragment of the mitochondrial COI gene (DNA barcode). The DNA barcodes of the species presented here were compared with those of all Pleurotinae available to us in BOLD, altogether clustering in 135 different BINs (Barcode Index Numbers). The results are discussed under the Molecular data section of each species. The barcodes used in this study are publicly available through the BOLD dataset DSPLEESP at dx.doi.org/10.5883/DS-PLEESP. Terminology for forewing markings and male and female genitalia, as well as methods and equipment used for dissecting and photographing adult specimens, are explained by Kaila et al. (2019) J. TABELL, M. HONEY, T. LEGER, M. MUTANEN, T. NUPPONEN & P. SIHVONEN 2SHILAP Revta. lepid., 53 (209) marzo 2025 SHILAP Revta. lepid., 53 (209) marzo 2025 45 and Tabell et al. (2019) and are not repeated here. Pleurotinae species are generally characterized by long and porrect labial palpi that are densely covered by long, piliform scales, with a straight, upward pointing 3rd segment. Morphological traits characterizing and delimiting Pleurotinae (Oecophoridae) are provided in Kaila et al. (2019). Taxonomy A review of seven Spanish Pleurota species Pleurota hebetella Ragonot, 1889 (Figures 1, 27, 51) Barcode Index Number: BOLD:ADA2839 Pleurota hebetella Ragonot, 1889. Bull. Soc. ent. Fr., (6)9, 130 Type locality: SPAIN: Albarracín and Cuenca. Paratype 1, coll. E. L. Ragonot (MNHN [not found)], designated by Back (1973), Albarracín, Cotype, male genitalia illustrated by Back (1973). Material studied: SPAIN, Aragón, prov. TERUEL, 3.5 km ENE Monteagudo del Castillo vill., 15301600 m, 2 1(DNA sample 24684 Lepid Phyl; DNA sample 24685 Lepid Phyl), 24-VI-2012, T. Nupponen leg.; same collecting data, 1 1(GP 5574 J. Tabell, DNA sample 24683 Lepid Phyl) but 25VI-2012; same collecting data, but 1500 m, N40.46847 W0.77984, 4 1(GP 5769 J. Tabell), 6-VII2016, J. Tabell leg. (coll. TAB). Diagnosis: P. hebetella is characterized by unicolorous ochreous brown forewing with dark grey costal and dorsal fringes. In the male genitalia, the large area of scobination of gnathos and the long posterior lobe of juxta are diagnostic. The female is unknown. Molecular data: Three specimens of P. hebetella were sequenced successfully, resulting in 658 bp (n=2) and 567 bp (n=1) DNA barcodes. The nearest neighbour to P. hebetella is P. rondaensis sp. nov., with a 3.13 % divergence. The barcodes of P. hebetella exhibit 0.36 % intraspecific variation. Description: Adult. Wingspan 13.5-15 mm. Labial palpus pale ochreous brown, ventrally mixed with off-white scales, apical half dark brown, about 6.5x as long as diameter of the eye (1st and 2nd palpomeres), 3rd palpomere 0.25x length of 1st and 2nd palpomeres. Antenna brown, hairy, 0.75x length of forewing. Tegula and thorax ochreous brown, frontal tuft, and neck slightly paler. Forewing narrow, ochreous brown, without longitudinal pale lines and dark spots, except for narrow pale ochre costal line from near base to 3/4. Cilia dark grey. Hindwing and cilia dark grey. Abdomen slightly lustrous, blackish grey, each segment with a transverse row of ochre scales. Male genitalia: Uncus thimble-shaped from ventral view, elongate, lined with several bristles of different length, distal margin concave. Gnathos 1.65x as long as uncus, funnel-shaped from ventral view, apex broadly rounded, with a shallow medial longitudinal crest, apical 2/3surfaced by transverse ridges and small nodules, apex with sharp spines. Valva upwards oblique, thimble-shaped, ventral margin concave; sacculus covered with long bristles. Anterior extension of juxta straight, robust; posterior lobe exceeding the apex of uncus, apex with a small tooth; valval lobe elongate, parallelsided, upwards oblique, apex evenly rounded, broadly covered with long bristles. Phallus arched, parallel-sided, with one weakly sclerotized cornutus and a group of numerous tiny spines, apical 1/4 dorsally covered with small spines and nodules. Female genitalia: Unknown. Biology: Unknown. The habitat in Teruel is a calcareous meadow. Distribution: Spain. Records from Portugal and France should be re-examined. Remarks: Back (1973) states that one paratype is housed in MNHN, but it has not been found there despite searching efforts (Sylvain Delmas, pers. comm.). The location of other type specimens is not known. The male genitalia of the paratype are illustrated and described by Back (1973), the female is unknown. The original description corresponds well with the specimens presented here, except for the different size (17 mm). It is possible that the original type series enclosed two or more different species, as was the case with P. amaurodoxa (see Remarks section of P. amaurodoxa). Back (1973) NEW AND POORLY KNOWN PLEUROTA HÜBNER, [1825] SPECIES FROM SPAIN SHILAP Revta. lepid., 52 (207) diciembre 2024 3 46 SHILAP Revta. lepid., 53 (209) marzo 2025 points out that specimens determined as P. hebetella in different collections are mostly misidentifications. Pleurota amaurodoxa Meyrick, 1935 (Figures 2-3, 28-29, 42, 51) Barcode Index Number: BOLD:AED0185 Pleurota amaurodoxa Meyrick, 1935. Exotic Microlep., 4(18-19), 593 Type locality: SPAIN: Burgos, Estepar. Lectotype 1, designated by Vives Moreno (2014) (NHMUK; examined from photographs). Material studied: SPAIN, Castilla y León, BURGOS, 8 km SE, 42.28170 -3.64025, 980 m, 4 1(GP 6124 J. Tabell; DNA sample 26327 Lepid Phyl; DNA sample 26329 Lepid Phyl), 1 0(GP 6349 J. Tabell, DNA sample 26328 Lepid Phyl), 4-VII-2019, J. Tabell leg.; same collecting data, 1 1, but 5VII-2019 (coll. TAB). Diagnosis: P. amaurodoxa is a small species which externally resembles the Spanish species P. hebetella and P. pyrenaella sp. nov. The male genitalia can be distinguished by shorter uncus and posterior lobe of juxta, and broader valval lobe. The female genitalia are similar to those of P. pyrenaella, but both apophyses are longer, the posterior signa are markedly longer, and the spines of anterior signum are longer. The female of P. hebetella is unknown. Molecular diagnosis: Three specimens of P. amaurodoxa were sequenced, resulting in 655, 654 and 651 bp DNA barcodes. The nearest neighbour to P. amaurodoxa is P. hebetella, with a 3.13 % divergence. The barcodes of P. amaurodoxa exhibit no intraspecific variation. Description: Adult. Wingspan 12-15.5 mm. Labial palpus mixed with off-white, brown and blackish brown scales, ventrally darker, about 5x as long as diameter of eye (1st and 2nd palpomeres), 3rd palpomere 0.3x length of 1st and 2nd palpomeres. Antenna brown, mixed with off-white scales, 0.85x length of forewing. Frontal tuft and neck brown, mixed with off-white, tegula and thorax ochre, mixed with off-white. Forewing narrow, covered by pale ochre, ochre-tipped and brown scales, without longitudinal lines and dark spots, except for a narrow off-white costal line from base to 2/3. Cilia brownish grey. Hindwing and cilia dark brownish grey. Abdomen slightly lustrous, dark brownish grey, each segment with a transverse row of ochre scales. Male genitalia: Uncus thimble-shaped from ventral view, elongate, lined with several bristles of different length, distal margin concave. Gnathos 1.75x as long as uncus, funnel-shaped from ventral view, tapered towards apex, with a shallow, long longitudinal median crest, medially surfaced by small nodules. Valva elongate, upwards oblique, thimble-shaped, ventral margin evenly curved. Anterior extension of juxta straight, well sclerotized; median part rounded; posterior lobe not reaching apex of uncus, apex with a small tooth; valval lobe club-shaped, moderately broad, apex evenly rounded, lined with several bristles. Phallus curved, parallel-sided, moderately narrow, with a narrow chain of small spines. Female genitalia: Papilla analis ovoid, narrow, covered with bristles of different length. Apophyses long; posterior apophysis 6x as long as papilla analis and 1.05x as long as anterior apophysis, which is 2.4x as long as segment 8. Segment 8 longitudinally elongated, rectangular, membranous, quadrate, distally sparsely covered with bristles, dorsolaterally reinforced by sclerotized band; branch of anterior apophysis apically fused, U-shaped; ventral longitudinal sclerotization narrow, shorter than segment 8. Antrum tubular, parallel-sided, anterior half sclerotized, colliculum short. Ductus bursae slightly expanded towards corpus bursae. Corpus bursae ovoid; anterior signum strongly arched, broad, protuberances long, thornlike; two leaf-shaped posterior signa with thornlike protuberance and strongly elongated base. Biology: Unknown. The specimens collected by the first author were netted around Genista sp. Distribution. Known only from northern Spain, near Burgos. Remarks: The species description by Meyrick was based on three specimens collected from Estepar, Burgos, in northern Spain. According to Vives Moreno (2014) the type series embraces three different species: P. pyropella ([Denis & Schiffermüller], 1775) (= P. iberica?), P. ericella (Duponchel, 1839) and P. amaurodoxa. The last specimen was selected as a lectotype by Vives Moreno (2014). J. TABELL, M. HONEY, T. LEGER, M. MUTANEN, T. NUPPONEN & P. SIHVONEN 4SHILAP Revta. lepid., 52 (207) diciembre 2024 SHILAP Revta. lepid., 53 (209) marzo 2025 47 Pleurota protasella Staudinger, 1883 (Figures 4-5, 30, 43, 51) Barcode Index Number: BOLD:ACY6183 Pleurota protasella Staudinger, 1883, Stettin Ent. Ztg., 44(4-6), 184-185 Type locality: SPAIN: Granada, Sierra de Alfacar. Lectotype [here designated]: “Origin.” [pink label],”Sierra de Alfacar m[ich] 80.” [pale blue label], “Paralectotypus” [red label], “ex coll. Staudinger” (MFNB, examined from photographs and barcode: DNA sample MFNLEP252, 658 bp[89n]). Material studied: SPAIN, Prov. GRANADA, Sierra Nevada, 1490 m, 4 km SW Güejar-Sierra, N37.135 W3.473, 3 1, 2 0(GP 5500 J. Tabell, DNA sample 24575 Lepid. Phyl; GP 5956 J. Tabell, DNA sample 24927 Lepid. Phyl), 2-VII-2015, J. Tabell leg.; Prov. Granada, Sierra Nevada 1620 m, 2 km NW Bayárcal, N37.040 W3.014, 4 1, 1 0(DNA sample 24574 Lepid. Phyl), 4-VII-2015, J. Tabell leg.; Granada, Sierra Nevada 2030 m, 1 km S Puerto de la Ragua, N37.104 W3.027, 3 1(GP 5471 J. Tabell, DNA sample 24572 Lepid. Phyl), 5-VII-2015, J. Tabell leg.; ALMERÍA, Sierra de los Filabres, Calar Alto 2130 m, N37.222 W2.545, 4 1(DNA sample 24573 Lepid. Phyl; DNA sample 24926 Lepid. Phyl), 5-VII-2015, J. Tabell leg.; ALICANTE, 9 km NNW Albatera, 460 m, N38.254 W0.913, 1 1 (DNA sample 25603 Lepid. Phyl), 21-V-2016, J. Tabell leg.; Alicante, 9.4 km NNW Albatera, 500 m, N38.256 W0.919, 2 1(GP 5694 J. Tabell), 22-V-2016, J. Tabell leg. (all coll. TAB). The species was not treated by Back (1973). Diagnosis: Characterized by broad white costal line, dark discal and discocellular spots and short 3rd palpomere of labial palpus. Very long posterior lobe of juxta and long chain of cornuti in the male genitalia, and the combination of long apophyses, long and broad antrum and long corpus bursae in the female genitalia distinguish P. protasella from other species. Molecular diagnosis: Seven specimens of P. protasella were sequenced, resulting in 658 bp DNA barcode sequences for all specimens. The nearest neighbour to P. protasella is the Moroccan species P. ternaria Tabell, 2019, with a 5.74 % divergence. The barcodes of P. protasella exhibit 1.71 % intraspecific variation, which may indicate cryptic diversity. Description: Adult. Wingspan 12.5-15.5 mm. Labial palpus 5.6x as long as diameter of eye (1st and 2nd palpomeres), 3rd palpomere 0.25x length of 1st and 2nd palpomeres, white, below broadly dark brown. Antenna hairy, pale brown, 0.65x length of forewing. Head, thorax and tegula off-white. Forewing white, scattered with pale fuscous, brown-tipped scales, blackish brown discal and discocellular spots distinct, between them a faint streak, another streak from base to plical spot, fringe line formed of several dark to blackish brown scales; costal line white, from base to 2/3, widest at middle; subcostal line greyish brown, mixed with white, apically scattered with a few blackish brown scales, gradually widened from near base to apex; cilia long, pale brown, basally and apically white. Hindwing pale grey, cilia pale grey, apically paler. Abdomen slightly lustrous, pale grey, each segment with a transverse row of ochre scales. Male genitalia: Uncus bell-shaped from ventral view, markedly shorter than gnathos, lined with few long bristles, apical half narrow. Gnathos funnel-shaped from ventral view, 1.55x length of uncus, gradually tapered towards apex, surfaced with small nodules medially, apex rounded, moderately broad. Valva upwards oblique; cucullus narrow, ventral margin medially concave, costa slightly concave; valval lobe narrow, finger-shaped, basally bulged, surfaced with a few long bristles apically. Anterior extension of juxta straight, median part rounded, posterior lobe narrow, very long, with a small apical tooth. Vinculum V-shaped. Phallus curved, slightly tapered towards apex, with an elongate plateshaped cornutus and a narrow chain of several spiniform cornuti, apical 1/5with small nodules dorsally. Female genitalia: Papilla analis ovoid, narrow, covered with bristles of different length. Apophyses long; posterior apophysis about 5x as long as papilla analis and 1.5x as long as anterior apophysis, which is 2x as long as segment 8. Segment 8 longitudinally elongated, rectangular, membranous, quadrate, distally very sparsely covered with bristles, dorsolaterally reinforced by sclerotized band; branch of anterior apophysis apically fused, U-shaped. Antrum tubular, long, posterior half densely covered by small spinules, anterior half more sclerotized. Ductus bursae NEW AND POORLY KNOWN PLEUROTA HÜBNER, [1825] SPECIES FROM SPAIN SHILAP Revta. lepid., 52 (207) diciembre 2024 5 48 SHILAP Revta. lepid., 53 (209) marzo 2025 membranous, short. Corpus bursae ovoid, long, with a large arched signum with two sclerotized wedge-shaped protuberances, and two leaf-shaped signa of equal size. Biology: Unknown. Distribution: Spain. Records from France should be re-examined. Remarks: The species description by Staudinger was based on four male specimens, but only three specimens labelled as paralectotypes were found in MFNB. These designations are unpublished, and according to the International Code of Zoological Nomenclature (ICZN 1999, Article 74.7) they are not valid. Consequently, one original specimen is designated herein as a lectotype. Pleurota glitzella (Staudinger, 1883) (Figures 6-7, 31, 51) Barcode Index Number:- Protasis glitzella Staudinger, 1883. Stettin Ent. Ztg., 44(4-6), 185 Type locality: SPAIN: Granada. Lectotype [here designated]: “Lectotypus 1” [red label], “Lectotypus n 9. 1Pleurota glitzella Stgr. Lvovsky det.”, ”Granada m[ich] 80” [pale blue label], “Origin.” [pink label] (MFNB, examined from photographs and barcode: DNA sample MFNLEP246, 658 bp [250n]). Material studied: SPAIN, ALMERÍA, Sierra de los Filabres, route Purchena-Senés, 1600 m, 1 1(GP 5637 J. Tabell, DNA sample 25437 Lepid Phyl [barcoding failed]), 16-VI-2007, Jan Sumpich leg. & coll. The species was not treated by Back (1973). Diagnosis: A small species, characterized by very short 3rd palpomere of labial palpus, which is concealed by hairy scales, and long cilia in the forewing with two distinct dark, broad, and slightly concave fringe lines. In the male genitalia, the shape of gnathos, valval lobe and strongly concave outer margin of cucullus distinguish P. glitzella from other species. The female is unknown. Molecular diagnosis: An old lectotype was sequenced successfully, resulting in a 658 bp DNA sequence [250n]. It exhibits 97.26 % similarity with the sequence of a Spanish still undetermined Pleurota species (n=3, private records). Description: Adult (faded to brown, originally greyer). Wingspan 11-13 mm (original description). Labial palpus white to pale brownish white above, 3rd palpomere not visible. Antenna pale brown. Head, thorax and tegula white. Forewing white, dorsal third covered with pale brown scales, blackish brown discal, discocellular and plical spots distinct; no costal line; subcostal line broad, brown, mixed with white. Fringe line V-shaped, formed of blackish brown scales, cilia long, white, with two distinct brown lines, lines slightly concave at dorsal area. Hindwing and cilia pale brown. Abdomen slightly lustrous, pale grey, each segment with a transverse row of ochre scales. Male genitalia: Uncus triangular from ventral view, lined with several long bristles. Gnathos fluke-shaped from ventral view, tapered towards apex, moderately short, 1.3x length of uncus, surfaced with small nodules medially. Valva upwards oblique; cucullus narrow, ventral margin medially concave, costa slightly bulged basally; valval lobe club-shaped, basally slightly bulged, apical half surfaced with several nodules and short bristles. Anterior extension of juxta straight and narrow, posterior lobe very long, with a small apical tooth. Vinculum U-shaped. Phallus curved, parallel-sided, with a robust plate-shaped cornutus and a long loose bundle of numerous spiniform cornuti. Female genitalia: Unknown. Biology: Unknown. Distribution: Southern Spain. Remarks: The designation by Lvovsky is unpublished, and according to the ICZN (1999, Article 74.7) it is not valid. Consequently, one original specimen is designated herein as a lectotype. Pleurota pleurotella (Staudinger, 1871) (Figures 8-10, 32, 44, 51) Barcode Index Number: BOLD:ACW2024 Protasis pleurotella Staudinger, 1871. Berl. Ent. Z., 14(3/4), 317 Type locality: SPAIN: Andalusia. Lectotype [here designated]: “Origin” [pink label], “ex Coll. Staudinger”, “Andalucia Kal.[isch]” [pale blue label], “Zool. Mus. Berlin” [pale brown label], J. TABELL, M. HONEY, T. LEGER, M. MUTANEN, T. NUPPONEN & P. SIHVONEN 6SHILAP Revta. lepid., 52 (207) diciembre 2024 SHILAP Revta. lepid., 53 (209) marzo 2025 49 “Lectotypus Pleurota pleurotella Stgr. design. Lvovsky” [red label] (MFNB; examined from photographs). Material studied: SPAIN, GRANADA, Sierra Nevada 700 m, 2 km N Cherin, 1 0, 28-III-1998, T. Nupponen leg.; ALICANTE, 3 km E San Miguel de Salinas, 1 0(GP 5951 J. Tabell), 16-IV-2009, J. Tabell leg.; Alicante, 6 km NW San Miguel de Salinas, 2 0(GP 5298 J. Tabell), 22-IV-2009, J. Tabell leg.; ALMERÍA, 1 km SW Tabernas, 370 m, 1 1(GP 5460 J. Tabell, DNA sample 23688 Lepid. Phyl), 7IV-2014, J. Tabell leg.; GRANADA, 10 km NE Baza, 700 m, N37.563 W2.709, 2 1(DNA sample 23701 Lepid. Phyl), 1 0, 8-IV-2014, J. Tabell leg.; 1 1, same collecting data, but 10-IV-2014; same collecting data, 1 1(GP 5308, DNA sample 23687 Lepid. Phyl), but 13-IV-2014; Granada, 7.5 km N Motril, 100 m, N36.811 W3.546, 1 1(GP 5409 J. Tabell), 10-IV-2014, J. Tabell leg.; Granada, 5.5 km N Cüllar, 870 m, N37.632 W2.571, 1 1, 12-IV-2014, J. Tabell leg. (all coll. TAB). The species was not treated by Back (1973). Diagnosis: From the outer appearance, P. pleurotella is similar to P. gallicella Huemer & Luquet, 1995, but the dark spots on the forewing are larger, and the 3rd palpomere of the labial palpus is not visible. The combination of a narrow cucullus, long valval lobe and long posterior lobe of juxta distinguish P. pleurotella from other species. In the female genitalia, the long segment 8 and the shape of signa are characteristic. Molecular diagnosis: Three specimens of P. pleurotella were sequenced successfully, resulting in 658 bp barcodes for all specimens. The nearest neighbour to P. pleurotella is the morphologically distinct P. candia Back, 1973, with a 7.77 % divergence. The barcodes of P. pleurotella exhibit no intraspecific variation. Description: Adult. Wingspan 13.5-16 mm. Labial palpus 5.8x as long as diameter of eye (1st and 2nd palpomeres), 3rd palpomere very short, entirely concealed by scales, greyish white, mixed with pale brown, below broadly dark brown. Antenna hairy in male, brown, 0.65x length of forewing. Head offwhite, thorax and tegula mixed with brown and grey. Forewing off-white, scattered with brownish grey scales, blackish brown discal, discocellular and plical spots large, fringe line formed of several blackish brown scales; costal line absent; subcostal line brownish grey, mixed with white, gradually widened from base to near apex, apically indistinctly edged; cilia long, off-white, with two grey lines. Hindwing pale grey, cilia pale grey with yellow tinge, apically white. Abdomen slightly lustrous, grey, each segment with a transverse row of ochre scales. Male genitalia: Uncus triangular from ventral view, lined with few long bristles. Gnathos basally fluke-shaped, distally finger-shaped from ventral view, 1.6x length of uncus, surfaced with small nodules medially, apex broadly rounded. Valva upwards oblique; cucullus narrow, ventral margin medially concave, costa slightly bulged basally; valval lobe narrow and long, club-shaped, basally slightly bulged, apical half more sclerotized, surfaced with few short bristles apically. Anterior extension of juxta straight, posterior lobe very long, with a small apical tooth. Vinculum trapezoid. Phallus curved, parallel-sided, with a robust plate-shaped cornutus and a loose bundle of several spiniform cornuti. Female genitalia: Papilla analis ovoid, narrow, apical half covered with bristles. Apophyses long; posterior apophysis 6.8x as long as papilla analis and 1.5x as long as anterior apophysis, which is 1.7x as long as segment 8. Segment 8 longitudinally elongated, membranous, quadrate, dorsolaterally reinforced by sclerotized band; branch of anterior apophysis apically fused, U-shaped, weekly sclerotized; ventral longitudinal sclerotization narrow, as long as segment 8. Antrum tubular, long, parallel-sided. Ductus bursae narrow. Corpus bursae ovoid, narrow, with one arched signum with two serrated protuberances, and two leaf-shaped signa with long, straight spine. Biology: Unknown. Distribution: Known from southern Spain. NEW AND POORLY KNOWN PLEUROTA HÜBNER, [1825] SPECIES FROM SPAIN SHILAP Revta. lepid., 52 (207) diciembre 2024 7 50 SHILAP Revta. lepid., 53 (209) marzo 2025 Remarks: The designation by Lvovsky is unpublished, and according to the ICZN (1999, Article 74.7) it is not valid. Consequently, one original specimen is designated herein as a lectotype. Pleurota sobriella (Staudinger, 1859) (Figures 11-13, 33, 45, 51) Barcode Index Number: BOLD:ADB3164 Anchinia sobriella Staudinger, 1859. Stettin ent. Ztg., 20(7-9), 245-246 Type locality: SPAIN: Granada. Lectotype [here designated]: “21/6” [pale brown label], “Origin” [pink label], “Lectotypus Pleurota sobriella Stgr. 1Lvovsky det.”, “Granada. m[ich] [pale blue label], “Lectotypus 1” [red label], “ex Coll. Staudinger”, “19” [pale brown label] (MFNB; examined from photographs). Material studied: SPAIN,JAÉN, Albánchez, 1 1(DNA sample 24672 Lepid Phyl), 8-VI-1994, F. Schepler leg. (coll. TAB); Aragón, TERUEL, Albarracín, 3 km N, 1 1(DNA sample 24812 Lepid Phyl [barcoding failed]), 12-VI-2004, T. Nupponen leg.; Aragón, Teruel, Albarracín, 1 1(GP 5338 J. Tabell, DNA sample 23657 [barcoding failed]), 17-VI-2004, J. Junnilainen leg.; Prov. Soria, SORIA, 30 [km] SW, El Temeroso, 1080 m, 1 0(DNA sample 24814 Lepid Phyl), 26-VI-2012, T. Nupponen leg.; Aragón, prov. TERUEL, Monteagudo del Castillo vill. 3.5 km ENE, 1 1(DNA sample 24811 Lepid Phyl), 14-VI-2008, K. Nupponen leg.; ibidem, 1530-1610 m, 1 0(DNA sample 24813 Lepid Phyl), 16VII-2012, T. Nupponen leg.; Aragón, Teruel, Monteagudo del Castillo 3.5 km ENE, 1550 m, N40.46847 W0.77984, 3 1(GP 6035 J. Tabell; GP 6036 J. Tabell; DNA sample 25577 Lepid Phyl), 3 0(GP 5756 J. Tabell; GP 5768 J. Tabell), 6-VII-2016, J. Tabell leg. (all coll. TAB). Diagnosis: P. sobriella is characterized by olive-brown forewing with narrow white lines. The shape of gnathos and valval lobe in the male genitalia, and thick and long apophyses in the female genitalia are distinguishing details. Molecular diagnosis: Five specimens of P. sobriella were sequenced successfully, resulting in 658 bp (n=3), 611 bp (n=1) and 572 bp (n=1) sequences. The nearest neighbour to P. sobriella is P. subnobilella with a 1.66 % divergence. The barcodes of P. sobriella exhibit 0.56 % intraspecific variation. Description: Adult. Wingspan 22-24 mm. Labial palpus ochre, ventrally mixed white and brown, dorsolaterally with a dark brown apical spot, about 7.3x as long as diameter of the eye (1st and 2nd palpomeres), 3rd palpomere 0.35x length of 1st and 2nd palpomeres. Antenna brown, basally mixed with white scales. Frontal tuft and neck ochre, tegula and thorax yellowish olive-brown. Costal line narrow, indistinctly edged, from near base to 2/3; subcostal line yellowish olive-brown, parallel-sided; median line white, narrow, not connected to dent of median line, which forms a separate narrow line from base to tornus; fringe line off-white; dorsal line narrow and short; median area yellowish olive-brown, in female paler. Cilia grey, apically off-white. Hindwing and cilia dark grey. Abdomen slightly lustrous, dark grey, each segment with a transverse row of ochre scales. Male genitalia: Uncus thimble-shaped from ventral view, lined with several bristles of different length, apex blunt. Gnathos 1.9x as long as uncus, from ventral view elongate, funnel-shaped, medially covered with small nodules, apical half narrow, parallel-sided, apex blunt. Valva elongate, upwards oblique; cucullus thimble-shaped. Anterior extension of juxta straight, short, median part rounded, posterior lobe extended to uncus, apex armed with a small tooth. Valval lobe well sclerotized, very broad, rounded apically, covered with several small nodules and short bristles apically. Phallus curved, tapered towards apex, apex pointed, with one robust plate-shaped cornutus surrounded by numerous tiny spines. Female genitalia: Papilla analis ovoid, covered with bristles of different length. Apophyses very long; posterior apophysis 10x as long as papilla analis and 1.6x as long as anterior apophysis. Segment 8 elongated longitudinally, rectangular, membranous, dorsolaterally reinforced by sclerotized band, lined with a few bristles distally, ventral longitudinal sclerotization narrow, as long as segment 8, branch of anterior apophysis narrow. Antrum tubular, short, membranous, anterior 1/3tapered and sclerotized. Ductus bursae membranous, moderately broad, parallel-sided. Corpus bursae ovoid, with one robust arched signum bearing two serrated, thorn-like protuberances, and two leaf-shaped signa. J. TABELL, M. HONEY, T. LEGER, M. MUTANEN, T. NUPPONEN & P. SIHVONEN 8SHILAP Revta. lepid., 52 (207) diciembre 2024 SHILAP Revta. lepid., 53 (209) marzo 2025 51 Biology: Unknown. Distribution: Spain. Remarks: The designation by Lvovsky is unpublished, and according to the ICZN (1999, Article 74.7) it is not valid. Consequently, one original specimen is designated herein as a lectotype. Pleurota nobilella Rebel, 1901 (Figures 14-15, 34, 51) Barcode Index Number: BOLD:ADI2705 Pleurota honorella Hb. v. nobilella Rebel, 1901. Dt. ent. Z. Iris, 13(2), 169 Type locality: SPAIN: Murcia. Lectotype [here designated] 0[abdomen missing] “Type” [red ink], “Origin.” [pink label], “ex coll. Staudinger” (MFNB, examined from photographs and barcode: DNA sample MFNLEP252, 658 bp[89n]). Material studied: SPAIN, ALICANTE, 9 km NNW Albatera, 460 m, N38.25317 W0.91450, 5 1(GP 5856 J. Tabell, DNA sample 25562 Lepid Phyl; GP 5901 J. Tabell, DNA sample 25563 Lepid Phyl; DNA sample 26200 Lepid Phyl), 21-V-2016, J. Tabell leg. (coll. TAB). Diagnosis: Brown subcostal line, which is markedly darker than the rest of the wing, distinguishes P. nobilella from P. honorella and P. subnobilella sp. nov. In the male genitalia, the broad posterior lobe of juxta is characteristic. The female genitalia are unknown. Molecular diagnosis: Three fresh specimens of P. nobilella were sequenced, resulting in 658 bp (n=2) and 652 bp (n=1) barcodes. In addition, two old type specimens were sequenced (see Remarks). The nearest neighbor to P. nobilella is the Corsican P. cyrniella Mann, 1855, with a 6.53 % divergence. Minimum and maximum genetic divergences between the lectotype and three P. nobilella specimens are 0.72 % and 0.9 %, respectively. Description: Adult. Wingspan 18-23.5 mm, 0lectotype 29 mm. Labial palpus pale grey, ventrally mixed white and brown, dorsolaterally with a dark brown apical spot, about 6.3x as long as diameter of the eye (1st and 2nd palpomeres), 3rd palpomere 0.25x length of 1st and 2nd palpomeres. Antenna dark brown. Frontal tuft and neck off-white, mixed with pale beige, tegula white, mixed with pale ochre, thorax white, medially mixed with pale ochre. Costal line white, from near base to 4/5, evenly tapered towards apex; subcostal line brown, evenly expanded towards apex; median line from base to apex, white, at junction of dent of median line narrow, then expanded, apical 1/3tapered, dent wedge-shaped, not reaching termen; fringe line indistinct, only slightly paler than median area; dorsal line parallelsided, white, from base to 1/3; median area pale ochreous yellow. Cilia pale brown. Hindwing and cilia brownish grey. Abdomen slightly lustrous, grey, each segment with a row of ochre scales. Male genitalia: Uncus thimble-shaped from ventral view, lined with several bristles of different length, apex blunt. Gnathos 1.7x as long as uncus, from ventral view elongate, funnel-shaped, medially covered with small nodules, gradually tapered towards apex, apex weakly beak-shaped. Valva elongate, upwards oblique, ventral margin evenly curved; cucullus narrow, apex slightly expanded. Juxta robust; anterior extension straight, short, median part rounded, posterior lobe broad, not extended to uncus, apex armed with a small tooth. Valval lobe broad, rounded apically, covered with several short bristles apically. Vinculum U-shaped. Phallus curved, parallel-sided, with one robust plate-shaped cornutus surrounded by numerous tiny spines. Female genitalia: Unknown. The female lectotype lacks the abdomen. The description by Back (1973) is not based on type material and is thus dubious. Biology: Unknown. Distribution: Known from south-eastern Spain, provinces of Murcia and Alicante. Remarks: P. nobilella was originally described as a variation of P. honorella (Hübner, [1813]), based on two specimens. Back (1973) upgraded it to a valid species, stating “Verbleib nicht nachgewiesen” [location is not known]. Two specimens found in MFNB bear “Origin”, “Type” and “ex coll. Staudinger” labels, and they indisputably are the original type specimens. The male genitalia are illustrated and described by Back (1973), the female genitalia are shortly described but not illustrated (from the specimen collected from Sierra de Espuña, province of Murcia). Based on the external appearance, the male and female type specimens are not conspecific. Also, NEW AND POORLY KNOWN PLEUROTA HÜBNER, [1825] SPECIES FROM SPAIN SHILAP Revta. lepid., 52 (207) diciembre 2024 9 58 SHILAP Revta. lepid., 53 (209) marzo 2025 of Pleurota species close to P. aristella (Linnaeus) from Morocco (Lepidoptera: Gelechioidea: Oecophoridae). Zootaxa, 4545(4), 451-477. https://doi.org/10.11646/zootaxa.4545.4.1 PMid:30790885 Rebel, H. (1901) Neue palaearctische Tineen. Deutsche entomologische Zeitschrift Iris, 13(2), 161-188. Tabell, J., Mutanen, M., & Sihvonen, P. (2019). Three morphologically and genetically confirmed new Pleurota species from Morocco (Lepidoptera: Gelechioidea: Oecophoridae: Pleurotinae). Zootaxa, 4624(3), 442-450. https://doi.org/10.11646/zootaxa.4624.3.12 PMid:31716219 Tabell, J., Wikström, B., Mutanen, M., Bruckner, H., & Sihvonen, P. (2021). Subspecies of Pleurota bicostella (Clerck, 1759) revisited and descriptions of nine new species in the P. bicostella species group (Lepidoptera: Gelechioidea: Oecophoridae: Pleurotinae). Zootaxa, 4941(4), 451-486. https://doi.org/10.11646/zootaxa. 4941.4.1 PMid:33756919 Tokár, Z., Lvovsky, A., & Huemer, P. (2005) Die Oecophoridae s.l. Mitteleuropas (Lepidoptera). Bestimmung, Verbreitung, Habitat, Bionomie. Frantisek Slamka. Vives Moreno, A. (2014). Catálogo sistemático y sinonímico de los Lepidoptera de la Península Ibérica, de Ceuta, de Melilla y de las islas Azores, Baleares, Canarias, Madeira y Salvajes (Insecta: Lepidoptera). Improitalia. *Jukka Tabell Martin Honey Laaksotie, 28 Natural History Museum FI-19600 Hartola Department of Life Sciences FINLANDIA / FINLAND Cromwell road E-mail: [email protected] GB-London SW7 5BD https://orcid.org/0000-0002-3477-5360 REINO UNIDO / UNITED KINGDOM E-mail: [email protected] Théo Leger https://orcid.org/0000-0002-7282-5074 Museum für Naturkunde Berlin Invalidenstraße, 43 Marko Mutanen D-10115 Berlin Ecology and Genetics research unit ALEMANIA / GERMANY University of Oulu E-mail: [email protected] P. O. Box, 3000 https://orcid.org/0000-0001-7330-3940 FI-90014 University of Oulu FINLANDIA / FINLAND Timo Nupponen E-mail: [email protected] Staffanintie, 10 A https://orcid.org/0000-0003-4464-6308 FI-02360 Espoo FINLANDIA / FINLAND Pasi Sihvonen E-mail: [email protected] Finnish Museum of Natural History https://orcid.org/0009-0003-8532-2171 University of Helsinki P.O. Box 17 FI-00014 Helsinki FINLANDIA / FINLAND E-mail: [email protected] https://orcid.org/0000-0003-2237-9325 *Autor para la correspondencia/ Corresponding author (Recibido para publicación / Received for publication 18-IV-2024 (Revisado y aceptado / Revised and accepted 26-VII-2024) (Publicado / Published 30-XII-2024) Derechos de autor: El autor(es). Este es un artículo de acceso abierto distribuido bajo los términos de la Licencia de Reconocimiento 4.0 Internacional de Creative Commons (CC BY 4.0), que permite el uso, distribución y reproducción sin restricciones en cualquier medio, siempre que se cite al autor original y la fuente. / Copyright: The author(s). This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. J. TABELL, M. HONEY, T. LEGER, M. MUTANEN, T. NUPPONEN & P. SIHVONEN 16 SHILAP Revta. lepid., 52 (207) diciembre 2024 30-III-2025) SHILAP Revta. lepid., 53 (209) marzo 2025 59 NEW AND POORLY KNOWN PLEUROTA HÜBNER, [1825] SPECIES FROM SPAIN SHILAP Revta. lepid., 52 (207) diciembre 2024 17 Figures 1-8. Adults of Pleurota spp. 1. P. hebetella Ragonot, 1. 2-3. P. amaurodoxa Meyrick. 2. 1lectotype. 3. 1. 4-5. P. protasella Staudinger. 4. 1lectotype. 5. 1. 6.-7. P. glitzella (Staudinger). 6. 1lectotype. 7. 1. 8. P. pleurotella (Staudinger), 1lectotype. 12 34 56 78 60 SHILAP Revta. lepid., 53 (209) marzo 2025 J. TABELL, M. HONEY, T. LEGER, M. MUTANEN, T. NUPPONEN & P. SIHVONEN 18 SHILAP Revta. lepid., 52 (207) diciembre 2024 Figures 9-16. Adults of Pleurota spp. 9-10. P. pleurotella (Staudinger). 9. 1. 10. 0. 11-13. P. sobriella (Staudinger). 11. 1lectotype. 12. 1. 13. 0. 14-15. P. nobilella Rebel. 14. 0lectotype. 15. 1. 16. P. sp., 1 syntype of P. nobilella. 9 10 11 12 13 14 15 16 SHILAP Revta. lepid., 53 (209) marzo 2025 61 NEW AND POORLY KNOWN PLEUROTA HÜBNER, [1825] SPECIES FROM SPAIN SHILAP Revta. lepid., 52 (207) diciembre 2024 19 Figures 17-21. Adults of Pleurota spp. 17. P. rondaensis sp. nov., 1holotype. 18-19. P. pyrenaella sp. nov. 18. 1holotype. 19. 0paratype. 20-21. P. balearica sp. nov. 20. 1holotype. 21. 0paratype. 17 18 19 20 21 62 SHILAP Revta. lepid., 53 (209) marzo 2025 J. TABELL, M. HONEY, T. LEGER, M. MUTANEN, T. NUPPONEN & P. SIHVONEN 20 SHILAP Revta. lepid., 52 (207) diciembre 2024 Figures 22-26. Adults of Pleurota spp. 22. P. malagaensis sp. nov., 1holotype. 23. P. grancanariella sp. nov., 1holotype. 24-26. P. subnobilella sp. nov. 24. 1holotype. 25. 1paratype. 26. 0paratype. 22 23 24 25 26 SHILAP Revta. lepid., 53 (209) marzo 2025 63 Figures 27-28. Male genitalia of Pleurota spp. 27. P. hebetella Ragonot, GP 5769 J. Tabell. 28. P. amaurodoxa Meyrick. lectotype. NEW AND POORLY KNOWN PLEUROTA HÜBNER, [1825] SPECIES FROM SPAIN SHILAP Revta. lepid., 52 (207) diciembre 2024 21 29 30 27 28 64 SHILAP Revta. lepid., 53 (209) marzo 2025 Figures 29-30. Male genitalia of Pleurota spp. 29. P. amaurodoxa Meyrick GP 6124 J. Tabell. 30. P. protasella Staudinger, GP 5694 J. Tabell. J. TABELL, M. HONEY, T. LEGER, M. MUTANEN, T. NUPPONEN & P. SIHVONEN 22 SHILAP Revta. lepid., 52 (207) diciembre 2024 29 30 SHILAP Revta. lepid., 53 (209) marzo 2025 65 Figures 31-32. Male genitalia of Pleurota spp. 31. P. glitzella (Staudinger), GP 5537 J. Tabell. 32. P. pleurotella (Staudinger), GP 5460 J. Tabell. NEW AND POORLY KNOWN PLEUROTA HÜBNER, [1825] SPECIES FROM SPAIN SHILAP Revta. lepid., 52 (207) diciembre 2024 23 31 32 66 SHILAP Revta. lepid., 53 (209) marzo 2025 Figures 33-34. Male genitalia of Pleurota spp. 33. P. sobriella (Staudinger), GP 5338 J. Tabell. 34. P. nobilella Rebel, GP 5901 J. Tabell. 33 34 33 J. TABELL, M. HONEY, T. LEGER, M. MUTANEN, T. NUPPONEN & P. SIHVONEN 24 SHILAP Revta. lepid., 52 (207) diciembre 2024 SHILAP Revta. lepid., 53 (209) marzo 2025 67 Figures 35-36. Male genitalia of Pleurota spp. 35. P. rondaensis sp. nov., paratype, GP 5705 J. Tabell. 36. P. pyrenaella sp. nov., holotype, GP 5710 J. Tabell. NEW AND POORLY KNOWN PLEUROTA HÜBNER, [1825] SPECIES FROM SPAIN SHILAP Revta. lepid., 52 (207) diciembre 2024 25 35 36 74 SHILAP Revta. lepid., 53 (209) marzo 2025 Figure 51. Neighbor-joining tree based on the barcoding fragment of the COI gene (p-distance model) of Spanish Pleurota species. “MM25823” etc. are BOLD sample IDs. J. TABELL, M. HONEY, T. LEGER, M. MUTANEN, T. NUPPONEN & P. SIHVONEN 32 SHILAP Revta. lepid., 52 (207) diciembre 2024