Contribution to Morphometrics and Ecology of Snow Trout (Schizothorax eurycephalus) and Stone Loach (Triplophysa ferganaensis)

3.02.4 Contribution to Morphometrics and Ecology of Snow Trout (Schizothorax eurycephalus) and Stone Loach (Triplophysa ferganaensis) Erkin Karimov, Otabek Omonov, Pieterjan Verhelst, Bakhtiyor K. Karimov, Martin Schletterer and Daniel S. Hayes Article https://doi.org/10.3390/fishes10080377 Academic Editor: Stylianos Somarakis Received: 13 June 2025 Revised: 3 July 2025 Accepted: 23 July 2025 Published: 4 August 2025 Citation: Karimov, E.; Omonov, O.; Verhelst, P.; Karimov, B.K.; Schletterer, M.; Hayes, D.S. Contribution to Morphometrics and Ecology of Snow Trout (Schizothorax eurycephalus) and Stone Loach (Triplophysa ferganaensis). Fishes 2025,10, 377. https://doi.org/ 10.3390/fishes10080377 Copyright: © 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/ licenses/by/4.0/). Article Contribution to Morphometrics and Ecology of Snow Trout (Schizothorax eurycephalus) and Stone Loach (Triplophysa ferganaensis) Erkin Karimov 1,2,† , Otabek Omonov 3 , Pieterjan Verhelst 4 , Bakhtiyor K. Karimov 3 , Martin Schletterer 2, * and Daniel S. Hayes 2,*,† 1 Department of Zootechnics and Veterinary, Tashkent State Agrarian University, Tashkent 100140, Uzbekistan; [email protected] 2 BOKU University, Institute of Hydrobiology and Aquatic Ecosystem Management, Department of Ecosystem Management, Climate and Biodiversity, 1180 Vienna, Austria 3Tashkent Institute of Irrigation and Agricultural Mechanization Engineers, National Research University, Tashkent 100000, Uzbekistan; [email protected] (O.O.); [email protected] (B.K.K.) 4Research Institute for Nature and Forest, 1000 Brussels, Belgium; pieterjan.ver[email protected] *Correspondence: [email protected] (M.S.); [email protected] (D.S.H.) †These authors contributed equally to this work. Abstract The mountainous rivers of Central Asia host diverse ichthyofauna threatened by increasing anthropogenic pressures, particularly water pollution, abstraction, and hydropower development. This study provides valuable morphometric and ecological data for Schizothorax eurycephalus (snow trout) and Triplophysa ferganaensis (stone loach) in the Shakhimardan River basin, Uzbekistan. S. eurycephalus exhibited positive allometric growth, while T. ferganaensis showed negative near-isometric growth. The mean Fulton’s Condition Factor was 1.0 for S. eurycephalus and 0.7 for T. ferganaensis, with site-specific variations. Strong correlations among morphometric parameters, particularly length–height relationships, support non-invasive monitoring techniques. Dietary analysis revealed S. eurycephalus was predominantly herbivorous, with around 70% algae consumption. Early sexual maturity was observed in S. eurycephalus males, whereas T. ferganaensis showed no clear maturity signs, but swollen bellies suggested ongoing or recent reproductive activity. These baseline morphometric and ecological data establish a solid foundation for future ecological assessments, conservation strategies, and the design and monitoring of mitigation measures to address anthropogenic impacts in this vulnerable region. Keywords: ichthyology; snowtrout; snow barbel; Schizothoracinae; Shohimardon; Margilansay River; Syr Darya River Key Contribution: This study provides valuable morphometric and ecological baseline data for S. eurycephalus and T. ferganaensis, including condition factor, length–height relationships, and data on sexual maturity. 1. Introduction The mountains of Central Asia, particularly the Tien Shan mountains, feature unique riverine ecosystems and a diverse native fish fauna adapted to cold-water, steep-sloped high-altitude ecosystems [ 1 ]. Snow trout (Schizothorax Heckel 1838) and loach (Triplophysa Rendahl 1933) are widespread in the region. Schizothorax are valuable species for touristic Fishes 2025,10, 377 https://doi.org/10.3390/fishes10080377 Fishes 2025,10, 377 2 of 12 (recreational) fishing and constitute a food source for local communities. Both genera fulfill important ecological roles [ 2 , 3 ]. However, Schizothorax and Triplophysa populations across the region are increasingly exposed to anthropogenic pressures, notably water abstraction for irrigational use and hydropower development, leading to deterioration and fragmentation of habitats [ 4 – 8 ]. Despite the ecological significance of many Central Asian fish species and their use as bioindicator for freshwater ecosystem health, detailed, species-specific morphometric, and ecological information remains limited (but see [ 7 , 9 , 10 ]). Morphometric data, including relationships between fish length, weight, width, and height, are valuable for understanding fish condition and fundamental aspects of their ecology [ 11 , 12 ]. Incorporating multiple body dimensions can yield more robust assessments of fish condition than traditional length–weight-only metrics [ 11 , 12 ]. Alongside morphometrics, initial observations on ecological parameters, such as diet composition [ 13 ] and indicators of sexual maturity [ 14 ], including the identification of potential spawning or nursery areas, can provide critical insights into resource utilization, reproductive strategies, and habitat requirements. Collectively, such morphometric and ecological datasets underpin effective fisheries management and conservation. The Aral Sea basin is home to three species of Schizothorax, each associated with a distinct sub-catchment—the Amu Darya, Syr Darya, and Zeravshan Rivers [3,15,16]. This study, conducted in a tributary system of the Syr Darya River, focuses on Schizothorax eurycephalus (Berg, 1932) and Triplophysa ferganaensis (Sheraliev and Peng, 2021) [ 7 , 17 ]. The objectives of this research are to (1) quantify the length, weight, width, and height relationships of the target species, and to (2) present ecological observations on diet composition and sexual maturity. Therefore, this work provides a crucial empirical basis for further applied studies and the development of ecologically sound conservation measures. 2. Materials and Methods 2.1. Study Area and Field Sampling This study was conducted in the Shakhimardan River basin, located south of the Fergana Valley and flowing northward into the Syr Darya River. Specifically, sampling was conducted in the tributary network of the Shakhimardan exclave, Uzbekistan, including the Koksu and Aksu Rivers, as well as the Shakhimardan River (Figure 1). These rivers are characterized by fast-flowing, cold waters and rocky substrates, typical habitats of snow trout, S. eurycephalus, and stone loach, T. ferganaensis. These two species are the sole inhabitants of the study area [2,8]. The morphological state of the river systems is near natural with only local bank protection measures. The Aksu River is a glacier-fed stream. The Koksu River’s flow is fed by underground water sources downstream of two lakes formed by natural earthen dams [ 18 ]. Apart from water abstractions for small-scale irrigation, the flow regimes are still largely intact. However, one diversion hydropower plant has recently been commissioned at the Koksu River [ 19 ]; another one at the Shakhimardan River downstream of the Aksu and Koksu River confluence is currently under construction. We conducted semi-quantitative, single-pass electrofishing fish surveys between 25 March and 1 April 2025 using a backpack generator (EFKO FEG 1500, EFKO Elektrofischfanggeräte GmbH, Leutkirch im Allgäu, Germany) and a stationary device (Electracatch WFC7 0–250 V/0–10 Amps DC control box, Electracatch International Ltd., Peterborough, United Kingdom) in combination with a Honda 2000i 2kVA generator. In total, we sampled 3.5 km, consisting of six sites along a longitudinal gradient. Site length ranged from 291 to 900 m, with a mean of 592 m. Two sites are located in Aksu River, three in Koksu River, and one in Shakhimardan River (Table 1). Fish were stunned with electric fishing gear, caught with dip nets, and transferred into holding tanks. After capture, each specimen Fishes 2025,10, 377 3 of 12 was measured to the nearest mm for total length (TL; from the tip of the snout to the end of the caudal fin), body height (H; maximum vertical body height), and body width (W; maximum horizontal body width) (Figure 2), and to the nearest 0.1 g for weight. Fish stocks were calculated as abundance per 100 m based on the sampled river length. Specimens were also sexed by putting gentle pressure on the abdomen to check for milt with the males and eggs with females. Body width and height measurements were obtained only for a subset of the specimens. After measurements, all fish were released back into the river at the site of capture.  Figure 1. Location of the study area (A) in Central Asia (UZ = Uzbekistan, KG = Kyrgyzstan, TJ = Tajikistan); (B) detailed map showing the sampling sites in the Aksu, Koksu, and Shakhimardan Rivers. Table 1. Sampling sites and site-specific catch rates for Schizothorax eurycephalus and Triplophysa ferganaensis. Site ID River River Section Coordinates 1Sampled River Length [m] No. of S. eurycephalus No. of T. ferganaensis A1 Aksu Upstream (Iordan) 39.96521, 71.76154 900 92 37 A2 Aksu Downstream (near confluence) 39.98743, 71.80612 670 48 9 K1 Koksu Upstream (of waterfall) 39.967845, 71.830784 291 2 0 K2 Koksu Mid-section 39.982223, 71.810865 571 10 0 K3 Koksu Downstream (near confluence) 39.990531, 71.806553 314 10 9 S1 Shakhimardan Downstream of Aksu and Koksu River confluence 40.007499, 71.792839 805 12 2 1Coordinate reference system: WGS84; coordinates refer to the downstream sampling point (see Figure 1). Fishes 2025,10, 377 4 of 12 Figure 2. Morphometric fish measurements, exemplified by Schizothorax eurycephalus. TL = total length, H = maximum body height, W = maximum body width. Fish illustration © Jennifer Clausen 2023, https://www.jacdraws.com/ (accessed 20 June 2025). For gut content analysis, three specimens caught from Koksu River in September 2021 were euthanized using clove oil as an anesthetic, and their gastrointestinal tracts were carefully dissected. Subsequently, the gut contents were separated into different categories (algae, macroinvertebrates, and miscellaneous) and weighed to determine the percentage of each category relative to the total gut content. For each species, two specimens were preserved in ethanol and deposited in the Fish Collection of the Natural History Museum Vienna, Austria: S. eurycephalus (NMW-101620) and T. ferganaensis (NMW-101621). All sampling was performed in accordance with ethical and legal guidelines (State Ministry of Ecology, Environmental Protection and Climate Change of the Republic of Uzbekistan, Approval Code: 02-02/6-1741). 2.2. Data Analysis We first conducted descriptive statistics for standardized fish abundance [ind./100 m] and morphometric parameters, i.e., total length [mm], weight [g], body width [mm], and body height [mm] for the entire dataset and each sampling site, respectively. To assess the linear relationships among morphometric traits, we performed a Pearson correlation analysis. A correlation matrix was visualized displaying pairwise scatterplots, Pearson correlation coefficients, histograms of individual variables with overlaid density curves, and 95% confidence ellipses for bivariate relationships. Fulton’s condition factor (K), also known as the coefficient of condition, was estimated as K=100 ∗W/L3, (1) where ‘W’ denotes the weight of the fish and ‘L’ its TL. While Fulton’s condition factor is typically used when fish have isometric growth, it remains valuable even when allometric growth is considered more appropriate [20]. The length–weight relationships (LWRs) and length–height relationships (LHRs) were calculated based on the formula W=aLb, (2) where ‘W’ represents the weight of fish in grams (or, in the case of LHR: height in centimeters), ‘L’ the TL in centimeters, ‘a’ is the scaling constant, and ‘b’ is the allometric coefficient (slope). The values of ‘a’ and ‘b’ were estimated by logarithm-based linear regression, represented as Log(W)=log(a)+b∗Log (L)(3) Fishes 2025,10, 377 5 of 12 following methodologies outlined by Froese [ 20 ] and Le Cren [ 21 ]. We calculated the 95% confidence limits for ‘a’ and ‘b’, along with the coefficient of determination (R2), using the equations from Sparre and Venema [22]. The growth pattern was classified based on the value of ‘b’: b= 3 constitutes isometric growth, which is when weight and length increase proportionally and small fish have the same shape as large specimens; b> 3 represents positive allometric growth, which is when larger fish have increased in weight more than in length; b< 3 is negative allometric growth, which is when larger fish have increased in length more than weight [20]. All statistical analyses and graphical visualizations were performed using Microsoft Excel 2021 and R version 4.3.0. 3. Results A total of 174 S. eurycephalus and 57 T. ferganaensis were captured across the six sampling sites (Table 1). The highest numbers of each species were recorded at the most upstream site in Aksu River (site A1). S. eurycephalus was present at all surveyed locations. In contrast, T. ferganaensis was not detected at the two most upstream sites in the Koksu River (K1–K2). In the Aksu River, both species were encountered at the upstream (A1) and downstream (A2) sites, with S. eurycephalus exhibiting greater abundance than T. ferganaensis at both sites. Only in the Koksu River at the downstream end (K3), S. eurycephalus and T. ferganaensis were caught in similar abundances. At the Shakhimardan site (S1), both species were present, with twelve S. eurycephalus and two T. ferganaensis captured (Table 1). Standardized fish density for S. eurycephalus ranged from 1.5 ind./100 m at S1 to 10.2 ind./100 m at A1 (Table 2). For T. ferganaensis, where present, catch rates varied from 0.5 ind./100 m at S1 to 4.1 ind./100 m at A1 (Table 2). Table 2. Standardized fish density, morphometrics, and condition factor for Schizothorax eurycephalus and Triplophysa ferganaensis per sampling site. Species Site ID Ind./100 m Total Length [mm], Mean (Min–Max) Weight [g], Mean (Min–Max) Height [mm], Mean (Min–Max) Width [mm], Mean (Min–Max) Fulton’s Condition Factor (K) S. eurycephalus A1 10.2 177.5 (91–320) 76.3 (6–387) 28.1 (15–56) 21.9 (10–50) 1.0 (0.7–1.6) A2 7.2 205.5 (39–422) 146.2 (1–839) 43.5 (21–58) 36.3 (18–50) 1.1 (0.6–1.7) K1 1.1 238.5 (234–243) 184.5 (169–200) 48.0 (45–51) 38.0 (35–41) 1.4 (1.3–1.4) K2 3.6 182.1 (144–253) 67.8 (25–158) 27.0 (19–37) 21.8 (16–32) 1.0 (0.8–1.2) K3 3.2 211.3 (91–277) 123.7 (7–255) 35.8 (15–52) 30.2 (11–45) 1.1 (0.9–1.3) S1 1.5 257.3 (214–295) 213.0 (101–346) 47.2 (37–59) 38.0 (29–51) 1.2 (1.0–1.4) T. ferganaensis A1 4.1 117.8 (76–144) 11.6 (2–19) 13.0 (9–16) 13.0 (10–18) 0.7 (0.5–0.9) A2 1.5 104.3 (43–143) 10.8 (1–24) 15.0 (10–20) 17.0 (15–20) 0.8 (0.5–1.3) K1 0 - - - - - K2 0 - - - - - K3 3.0 105.0 (77–129) 10.2 (4–18) 13.2 (8–19) 12.8 (7–19) 0.8 (0.6–1.1) S1 0.5 117.0 (110–124) 12.5 (8–17) 14.0 (14–14) 16.0 (16–16) 0.7 (0.6–0.9) 3.1. Morphometrics Fish body metrics (length, weight, width, height) were all positively correlated with R ≥ 0.91 for all comparisons in S. eurycephalus (Figure 3a) and R ≥ 0.81 in T. ferganaensis. For the latter, correlations between fish length, weight and width were highest (R > 0.90). Fishes 2025,10, 377 6 of 12 Correlations with R < 0.90 were largely found to be related to fish height (Figure 3b). Considering the high correlations, we focus on selected parameters in the section below (see Tables 2and 3). Figure 3. Pairwise correlation matrix for morphometric measurements of (a)Schizothorax eurycephalus (n= 174 for length and weight, n= 88 for width and height) and (b)Triplophysa ferganaensis (n= 57 for length and weight, n= 18 for width and height). Scatterplots below the diagonal display the bivariate relationships between variables, with Pearson correlation coefficients shown above the diagonal. Histograms on the diagonal depict the distribution of each variable, overlaid with density curves. Ellipses represent 95% confidence intervals for the correlation in each bivariate plot. The red line is a smoothed density curve; the red dot represents the mean of the bivariate distribution for each pair of variables. All variables are measured in millimeters, except for weight measured in grams. Table 3. Length–weight relationship of Schizothorax eurycephalus and Triplophysa ferganaensis. Species nTotal Length Range [mm] Weight Range [g] a (95% CI) b (95% CI) R2 S. eurycephalus 174 39–422 1–839 0.007 (0.005–0.008) 3.130 (3.068–3.192) 0.98 T. ferganaensis 57 43–144 1–24 0.011 (0.006–0.019) 2.815 (2.573–3.057) 0.91 S. eurycephalus specimens from S1 exhibited the largest mean length (257.3 mm) and weight (213 g), while those from the mid-section of Koksu River (K2) were the smallest (mean TL = 182.1 mm) and lightest fish (mean weight = 67.8 g). The broadest range of observed lengths (39–422 mm) and weights (1–839 g) for S. eurycephalus was recorded at A2. Overall, the data show that the weight of S. eurycephalus generally increases from upstream to downstream, with the exception of K1, where two fish ready to spawn were caught (Figure 4a). Fulton’s condition factor (K) for S. eurycephalus was highest at K1 (mean = 1.4) and S1 (mean = 1.2), and lowest at K2 (Table 2). Site A2 exhibited the highest variability, ranging from 0.6 to 1.7 (Table 2; Figure 4b). The overall mean value was 1.0. Mean total lengths for T. ferganaensis ranged from 104.3 mm at site A2 to 117.8 mm at A1, with mean weight ranging from 10.2 g (K3) and 12.5 g (S1) (Table 2; Figure 4a). Even though the mean fish weight was the lowest at site K3, this location including A2 featured the highest condition factor of K = 0.8. The lowest condition factor of T. ferganaensis was at sites A1 and S1 with K = 0.7 (Table 2; Figure 4b). The overall mean value was 0.7. Fishes 2025,10, 377 7 of 12 Figure 4. (a) Fish weight and (b) Fulton’s condition factor by sampling site and species. Grey dots represent statistical outliers, i.e., values that fall outside 1.5 times the interquartile range. Table 3presents the statistics related to the length–weight relationship estimates, including length and weight ranges and confidence intervals for estimated parameters. Table 4contains the statistics related to the length–height relationship estimates. Table 4. Length–height relationship of Schizothorax eurycephalus and Triplophysa ferganaensis. Species nTotal Length Range [mm] Height Range [mm] a (95% CI) b (95% CI) R2 S. eurycephalus 88 91–325 15–59 0.119 (0.0945–0.15) 1.126 (1.049–1.204) 0.91 T. ferganaensis 18 76–139 8–20 0.037 (0.008–0.181) 1.251 (0.914–1.588) 0.79 3.2. Insights into Sexual Maturity and Diet Of the 174 S. eurycephalus individuals examined, 23% (n= 40) exhibited milt upon gentle abdominal pressure, indicating male sexual maturity. No females releasing eggs were observed during this procedure. The smallest male S. eurycephalus found to be sexually mature measured 77 mm TL and weighed 3 g, although the majority of mature males were >100 mm TL. Of the 57 T. ferganaensis individuals examined, only three (5%) exhibited milt, with the smallest mature male measuring 122 mm TL and weighing 13 g. Except for these Fishes 2025,10, 377 8 of 12 three individuals, we did not observe clear signs of sexual maturity in T. ferganaensis during our sampling campaign. However, we noted that their bellies appeared noticeably more swollen compared to other seasons, suggesting that maturation was underway or that the reproductive season had already started for this species. Preliminary gut content analysis was conducted on three S. eurycephalus specimens caught in Koksu River during September 2021 sampling. This examination revealed a diet primarily composed of algae, which accounted for approximately 70% of the gut contents by volume. Macroinvertebrate remains constituted a secondary dietary component, comprising about 28% by volume. The remaining 2% consisted of miscellaneous, undefined material. 4. Discussion Foundational morphometric data for S. eurycephalus and T. ferganaensis, two ecologically important species in the mountainous river systems of the Fergana Valley, Central Asia, remain scarce or undocumented, despite the urgent need for species-specific information to inform conservation and monitoring in this rapidly changing region [ 23 , 24 ]. This study addresses this gap by quantifying relationships of fish length, weight, height, and width and providing ecological observations on diet composition and sexual maturity for these fishes. These findings provide valuable data for future applied research and the development of ecologically sound conservation strategies. S. eurycephalus occurs in the entire Shakhimardan River basin. T. ferganaensis, however, is absent from the mid and upper sections of Koksu River [ 23 ]. Standardized fish density of S. eurycephalus was highest in the two Aksu River sites, as well as in the downstream reach of Koksu River. At both Aksu River sites, we detected aggregations of S. eurycephalus, which have gathered for upstream spawning migration. Interestingly, the two specimens caught in the upstream section of Koksu River, exhibited the highest condition factor; their bellies were thick, suggesting egg development, although the sex could not be determined. Moreover, both specimens were caught in a river section where no fish could be documented in previous surveys due to an artificial waterfall blocking upstream movements [ 23 ]. The partial removal of the waterfall in early 2025 seemed to have allowed the first upstream migration of S. eurycephalus since many decades. Fish specimens with the highest condition factors, similar to those in the Koksu, were also recorded in the Aksu River, further suggesting egg development in these well-conditioned individuals. The absence of T. ferganaensis from the Koksu River’s upstream and mid-sections suggests specific habitat preferences or limiting factors for this species in those reaches [2,8]. The length–weight relationship for S. eurycephalus indicates positive allometric growth, meaning that individuals become proportionally heavier for their length as they grow [ 2 ]. While positive allometry is common in many fishes, the genus Schizothorax exhibits considerable plasticity in growth patterns, with isometric or negative allometric growth reported for other species or populations under differing environmental conditions [ 2 , 25 , 26 ]. This variability highlights the importance of establishing population-specific baselines, as growth trajectories can be influenced by local factors such as food availability, water temperature regimes, and flow velocity [ 2 , 27 ]. For T. ferganaensis, the length–weight relationship indicates negative allometric growth, meaning that as individuals mature, their length increases more than their weight [ 20 ]. However, the confidence interval overlaps with isometric growth, indicating that the pattern is negative near-isometric. The coefficient of determination was low for the length–height relationship of T. ferganaensis, likely attributable to the limited number of specimens measured for these parameters. This suggests a need for further research with a larger sample size to establish a more robust length–height relationship for this species.