Supplementary material 1 from: Gao H-R, Wang C, Chen W-X, Li J-J, Zhang H-R, Li Y-J (2025) Across desert and island: Phylogeny of two thin stick insects from China with taxonomic insights into the clade "Gratidiini II" (Phasmatodea, Clitumninae). ZooKeys 1260: 149-160. https://doi.org/10.3897/zookeys.1260.143038

Table S1. Mitogenomes used for mitogenome phylogeny in this study. Species Genbank number Order Family Subfamily Sclerophasma paresisensis DQ241798 Mantophasmatodea - - Grylloblatta sculleni DQ241796 Grylloblattodea - - Parasipyloidea carinata PP432625 Phasmatodea Lonchodidae Necrosciinae Pachyscia longicauda PP654375 Phasmatodea Lonchodidae Necrosciinae Stheneboea repudiosa OQ682531 Phasmatodea Lonchodidae Lonchodinae Dryococelus australis AP018522 Phasmatodea Lanceocercata - Entoria okinawaensis AB477459 Phasmatodea Phasmatidae Clitumninae Phraortes illepidus AB477460 Phasmatodea Lonchodidae Lonchodinae Pulchriphyllium giganteum AB477461 Phasmatodea Phylliidae Phylliinae Orestes mouhotii AB477462 Phasmatodea Heteropterygidae Dataminae Ramulus irregulariterdentatus AB477463 Phasmatodea Phasmatidae Clitumninae Phraortes sp. Iriomote Island AB477464 Phasmatodea Lonchodidae Lonchodinae Phraortes sp. Miyako Island AB477465 Phasmatodea Lonchodidae Lonchodinae Micadina phluctainoides AB477466 Phasmatodea Lonchodidae Necrosciinae Lanceocercata sp. AB477467 Phasmatodea Lanceocercata - Heteropteryx dilatata AB477468 Phasmatodea Heteropterygidae Heteropteryginae Neohirasea japonica AB477469 Phasmatodea Lonchodidae Necrosciinae Sipyloidea sipylus AB477470 Phasmatodea Lonchodidae Necrosciinae Megacrania alpheus adan AB477471 Phasmatodea Lanceocercata - Extatosoma tiaratum AB642680 Phasmatodea Lanceocercata - Timema californicum DQ241799 Phasmatodea Timematidae - Ramulus hainanense FJ156750 Phasmatodea Phasmatidae Clitumninae Bacillus atticus GU001955 Phasmatodea Bacillidae Bacillinae Bacillus rossius GU001956 Phasmatodea Bacillidae Bacillinae Cryptophyllium tibetense KX091862 Phasmatodea Phylliidae Phylliinae Calvisia medogensis KY124330 Phasmatodea Lonchodidae Necrosciinae Megalophasma granulatum KY124331 Phasmatodea Lonchodidae Lonchodinae Cryptophyllium tibetense KY124332 Phasmatodea Phylliidae Phylliinae Micadina brachyptera MT025192 Phasmatodea Lonchodidae Necrosciinae Pharnaciini sp. MT025193 Phasmatodea Phasmatidae Pharnaciinae Cryptophyllium westwoodii MW229063 Phasmatodea Phylliidae Phylliinae Orestes guangxiensis MW450873 Phasmatodea Heteropterygidae Dataminae Peruphasma schultei MW450874 Phasmatodea Pseudophasmatidae Pseudophasmatinae Phryganistria guangxiensis MW450875 Phasmatodea Phasmatidae Pharnaciinae Eurycantha calcarata MW915467 Phasmatodea Lonchodidae Lonchodinae Orthomeria smaragdinum MZ312645 Phasmatodea Aschiphasmatidae Aschiphasmatinae Nanhuaphasma hamicercum MZ312646 Phasmatodea Aschiphasmatidae Aschiphasmatinae Neohirasea stephanus OL405132 Phasmatodea Lonchodidae Necrosciinae Sosibia gibba OM257176 Phasmatodea Lonchodidae Necrosciinae Sosibia ovata OM257177 Phasmatodea Lonchodidae Necrosciinae Phraortes lii ON493672 Phasmatodea Lonchodidae Lonchodinae Sungaya inexpectata OQ682523 Phasmatodea Heteropterygidae Obriminae Carausius sp. OQ682524 Phasmatodea Lonchodidae Lonchodinae Lopaphus albopunctatus OQ682525 Phasmatodea Lonchodidae Necrosciinae Lopaphus sphalerus OQ682526 Phasmatodea Lonchodidae Necrosciinae Marmessoidea bispina OQ682527 Phasmatodea Lonchodidae Necrosciinae Phraortes lianzhouensis OQ682528 Phasmatodea Lonchodidae Lonchodinae Pulchriphyllium bioculatum OQ682529 Phasmatodea Phylliidae Phylliinae Pulchriphyllium giganteum OQ682530 Phasmatodea Phylliidae Phylliinae Carausius morosus PP230539 Phasmatodea Lonchodidae Lonchodinae Neohirasea hongkongensis PP437079 Phasmatodea Lonchodidae Necrosciinae Macellina souchongia PQ469774 Phasmatodea Phasmatidae Clitumninae Sceptrophasma bituberculatum PQ469775 Phasmatodea Phasmatidae Clitumninae Table S2. Datasheet of Clitumninae used for standard molecular markers phylogeny in this study. Species Regions ID 18S 28S H3 COI COII 12S 16S Dares murudensis SB0329 MN925360 MN925489 MN925617 MN925733 MN925858 MN925079 MN925222 Haaniella gorochovi SB0136 MN925280 MN925418 MN925543 MN925670 MN925784 MN925004 MN925137 Mearnsiana bullosa SB0287 MN925344 MN925477 MN925604 MN925721 MN925846 MN925064 MN925207 Bacillus atticus atticus Palearctic AATcug1 AF038226 Bacillus atticus carius Palearctic ACAner1 AF038228 Bacillus atticus cyprius Palearctic ACYepy1 AF038227 Bacillus grandii benazzii Palearctic GBEtbe2 AF148315 Bacillus grandii grandii Palearctic GGRcgc6 AF148313 Bacillus grandii maretimi Palearctic GMAmar3 AF038224 Bacillus lynceorum Palearctic LYNcas1 AF038219 Bacillus whitei Palearctic WHIpma2 AF038213 Bacillus atticus Palearctic BAL4 OK333683 OK314536 OK324157 OK314810 Bacillus rossius Palearctic BAL3 OK333682 OK314624 OK324156 OK314806 Clonopsis gallica Palearctic CGAlau1 AF096287 Clonopsis maroccana Palearctic fTAR6 GQ370575 Clonopsis soumiae Palearctic fTAR64 GQ370577 Clonopsis felicitatis Palearctic CLO2 OK314556 OK324158 OK314839 Leptynia attenuata Palearctic f-APO502 AF241443 Leptynia caprai Palearctic f-AMA460 AF241439 Leptynia montana Palearctic LET1 OK333686 OK314686 OK324162 OK314713 Pijnackeria originis Palearctic SB0316 OK324238 Pijnackeria recondita Palearctic PVRFAS0001 KT799537 KT799543 Pijnackeria recondita Palearctic PVRFAS0028 KT799542 KT799548 Pijnackeria hispanica Palearctic PIJ1 OK333708 OK314673 OK324181 OK314727 Pijnackeria lelongi Palearctic HAL386 MW138562 MW138712 MW138481 MW138635 Pijnackeria originis Palearctic HTIS637 MW138566 MW138715 MW138485 MW138639 Pijnackeria masettii Palearctic HAV100 MW138563 MW138482 MW138636 Pijnackeria barbarae Palearctic HEP494 MW138564 MW138713 MW138483 MW138637 Macynia labiata Afrotropical MAC1 OK314657 Gratidia sp. 1 Afrotropical STI11 KT426686 KT426592 KT426631 KT426621 Gratidia sp. 2 Afrotropical STI15 FJ474177 FJ474256 FJ474333 FJ474403 Gratidiidae sp. 1 Afrotropical SB0215 OK333761 OK314625 OK324233 OK314764 OK333598 OK333446 Gratidiidae sp. 2 Afrotropical SB0456 OK333970 OK333809 OK314644 OK324283 OK314721 OK333644 OK333495 Gratidiidae sp. 3 Afrotropical SB0458 OK333971 OK333810 OK314649 OK324284 OK314850 OK333645 OK333496 Phalces tuberculatus Afrotropical PHT1 KT426682 KT426648 KT426619 Zehntneria mystica Afrotropical ZEM1 FJ474176 FJ474255 FJ474332 FJ474402 Clonaria conformans Oriental GRC1 FJ474132 FJ474210 FJ474288 FJ474363 Clonaria fritzschei Oriental WS021 KJ024562 KJ024500 KJ024453 Clonaria fritzschei Oriental SB0497 OK333985 OK333824 OK314684 OK324297 OK333657 Clonaria luethyi Oriental SB0496 OK333984 OK333823 OK314602 OK324296 OK314769 OK333656 OK333509 Clonaria natalis Afrotropical WS120 MK291794 MK291869 MK291565 MK291641 MK291716 Clonaria sp. 1 Afrotropical WS066 MK291769 MK291844 MK291541 MK291616 MK291692 Clonaria sp. 2 Afrotropical SB0193 MN925307 MN925439 MN925567 MN925693 MN925810 MN925026 Clonaria sp. 3 Afrotropical SB0212 OK333919 OK333759 OK314639 OK324231 OK314828 OK333596 OK333444 Clonaria sp. 4 Afrotropical SB0214 OK333920 OK333760 OK314676 OK324232 OK314834 OK333597 OK333445 Clonaria sp. 5 Oriental SB0498 OK333986 OK333825 OK314618 OK324298 OK314820 OK333658 OK333510 Clonaria sp. 6 Afrotropical SB0511 OK333988 OK333827 OK314641 OK314742 Clonaria sp. 7 Afrotropical CLN4 OK314678 OK314758 Cuniculina cuniculus Oriental WS146 MK291815 MK291890 MK291584 MK297282 MK291660 MK291735 Cuniculina sp. Oriental SB0318 MN925356 MN925488 MN925615 MN925732 MN925857 MN925076 MN925219 Entoria sp. Oriental SB0355 OK333928 OK314675 OK324243 OK314841 OK333607 OK333453 Entoria koshunensis Oriental ENK1 OK333685 OK314630 OK324161 Lobofemora bidoupensis Oriental SB0354 OK333927 OK333767 OK314627 OK324242 OK314774 OK333606 OK333452 Lobofemora scheirei Oriental SB0163 MN925299 MN925432 MN925560 MN925803 MN925156 Lobofemora scheirei Oriental SB0196 OK333916 OK333756 OK314593 OK324228 OK314857 OK333593 OK333441 Lobofemora sp. Oriental SB0399 OK333950 OK333789 OK314597 OK324264 OK314747 OK333627 OK333474 Macellina souchongia Oriental GHR005 PQ111436 PQ111451 PQ112637 PQ469775 Macellina dentata Oriental SB0084 MN925261 MN925399 MN925527 MN925656 MN924987 MN925118 Medaura jobrensis Oriental SB0378 OK333943 OK333782 OK314576 OK324258 OK314812 OK333621 OK333467 Medaura scabriuscula Oriental SB0352 OK333925 OK314692 OK324240 OK314784 OK333604 OK333450 Medauroidea extradentata Oriental WS33 AY121173 AY125313 AY125256 KJ024503 KJ024458 Medauroidea romantica Oriental SB0353 OK333926 OK333766 OK314574 OK324241 OK314716 OK333605 OK333451 Medauroidea sp. 1 Oriental SB0132 OK333902 OK333740 OK314621 OK324213 OK314726 OK333580 OK333427 Medauroidea sp. 2 Oriental SB0181 OK333912 OK333751 OK314702 OK324223 OK314771 OK333588 OK333437 Medauromorpha foedata Oriental SB0182 MN925302 MN925435 MN925563 MN925689 MN925806 MN925021 MN925159 Medauromorpha regina Oriental SB0086 OK333892 OK333730 OK314583 OK324203 OK314741 OK333571 OK333418 Parapachymorpha spinigera Oriental WS080 MK291775 MK291850 MK291547 MK297254 MK291622 MK291698 Parapachymorpha spinosa Oriental GB109 MK291776 MK291851 MK291548 MK297255 MK291623 MK291699 Parapachymorpha zomproi Oriental PAZ1 OK333706 OK314648 OK324179 OK314753 Pterulina distinctissima Oriental SB0398 OK333949 OK333788 OK314691 OK314796 OK333626 OK333473 Ramulus artemis Oriental WS046 KJ024416.1 KJ024395.1 KJ024532.1 KJ024567.1 KJ024460.1 Ramulus irregulariterdentatum Sino-Japanese SB0501 OK314643 OK324299 Ramulus mikado Sino-Japanese SB0370 OK333936 OK333775 OK314539 OK324251 OK314786 OK333614 OK333460 Ramulus nematodes Oriental SB0346 MN925369 MN925497 MN925628 MN925741 MN925865 MN925088 MN925230 Ramulus sp. 1 Oriental SB0347 OK333924 OK333765 OK314668 OK324239 OK314824 OK333603 OK333449 Ramulus sp. 2 Oriental SB0188 OK333914 OK333754 OK314541 OK324226 OK314836 OK333591 OK333440 Ramulus thaii Oriental RAT1 FJ474166 FJ474244 FJ474322 FJ474391 Rhamphophasma spinicorne Oriental WS058 MK291764 MK291839 MK291536 MK291687 Sceptrophasma bituberculatum Palearctic GHR003 PQ111431 PQ111449 PQ112636 PQ469774 Sceptrophasma hispidulum Oriental WS27 AY121167 AY125307 AY125250 KJ024563 KJ024455 Sceptrophasma langkawicense Oriental WS26 AY121166 AY125306 AY125249 KJ024454 Sceptrophasma sp. Oriental SB0517 OK333829 OK314667 Table S3. Best partitioning scheme and best-fitting models of mitochondrial genes selected by PartitionFinder2 with BIC for mitogenomes phylogeny analysis. Subset Subset partitions Best Model P1 nad1_codon1, nad5_codon1, nad4_codon1, nad4L_codon1 GTR+I+G P2 nad5_codon2, nad4L_codon2, nad1_codon2, nad4_codon2 GTR+I+G P3 nad5_codon3, nad4L_codon3, nad1_codon3, nad4_codon3 GTR+I+G P4 cox1_codon1 GTR+I+G P5 cox1_codon2 GTR+I+G P6 CytB_codon3, cox1_codon3 GTR+I+G P7 atp8_codon1, nad2_codon1, nad6_codon1, nad3_codon1, atp6_codon1 GTR+I+G P8 atp8_codon2, nad6_codon2, nad2_codon2 GTR+I+G P9 nad2_codon3, atp8_codon3, cox3_codon3, atp6_codon3, cox2_codon3, nad3_codon3, nad6_codon3 HKY+I+G P10 CytB_codon1, cox2_codon1, cox3_codon1 GTR+I+G P11 nad3_codon2, atp6_codon2, CytB_codon2, cox2_codon2, cox3_codon2 GTR+I+G P12 rrnL GTR+I+G P13 rrnS GTR+I+G Table S4. Best partitioning scheme and best-fitting models of standard molecular markers selected by PartitionFinder2 with AICc for 7 standard molecular markers phylogeny analysis. Subset Subset partitions Best Model P1 COX1_codon1 GTR+I+G P2 COX1_codon2 TIM+I+G P3 COX1_codon3, COX2_codon3 GTR+I+G P4 COX2_codon1 GTR+I+G P5 COX2_codon2 TVM+I+G P6 H3_codon1 TIM+G P7 H3_codon2 JC+I P8 H3_codon3 TRNEF+G P9 12S GTR+I+G P10 16S GTR+I+G P11 18S GTR+I+G P12 28S GTR+I+G Table S5. Gene order and organization of the mitochondrial genomes of Sceptrophasma bituberculatum (Sb) and Macellina souchongia (Ms). Gene Strand Length (bp) Sb/Ms trnI forward 66/68 trnQ reverse 69/69 trnM forward 67/66 nad2 forward 1017/999 trnW forward 65/65 trnC reverse 64/64 trnY forward 68/66 cox1 forward 1539/1539 trnL forward 64/64 cox2 forward 687/705 trnK forward 70/70 trnD forward 66/65 atp8 forward 156/156 atp6 forward 678/678 cox3 forward 789/789 trnG forward 63/63 nad3 forward 366/366 trnA forward 64/65 trnR forward 64/64 trnN forward 65/65 trnS forward 68/68 trnE forward 66/66 trnF reverse 65/66 nad5 reverse 1713/1713 trnH reverse 63/63 nad4 reverse 1332/1332 nad4l reverse 285/273 trnT forward 63/65 trnP reverse 67/64 nad6 forward 474/474 cob forward 1134/1134 trnS forward 69/67 nad1 reverse 958/966 trnL reverse 66/67 rrnL forward 1237/1266 trnV reverse 68/69 rrnS forward 758/760 control region forward 3025/1686