Supplementary material 1 from: Campbell MA, Perry SC, Yearwood KN, Auringer G, Buckmaster N, Finger AJ (2025) Evolutionary relationships of Fish Lake Valley Tui Chub Siphateles obesus ssp. (Teleostei, Cypriniformes, Leuciscidae) and a new genus of leuciscid minnows from the Alvord Basin, western United States. ZooKeys 1261: 39-67. https://doi.org/10.3897/zookeys.1261.151636

Supplementary Figure S1 Maximum Likelihood phylogeny of mitochondrial cytochrome (cytb) data featuring ‘Siphateles bicolor’. Tip labels are GenBank accession numbers for the sequences with results of species delimitation of ‘S. bicolor’ indicated for six clusters. For ‘S. bicolor’ labeled sequences, geographic information is provided. Nodal support is indicated by gray circles for bootstrap support (BS) > 90%, white circles for 90% > BS > 75% and not indicated for BS < 75%. Supplementary Figure S2 Maximum Likelihood phylogeny of Siphateles generated from 2,649 SNPs considered variable by IQ-TREE2 with individual sampling locations indicated at tips. Nodal support is indicated by gray circles for bootstrap support (BS) > 90%, white circles for 90% > BS > 75% and not indicated for BS < 75%. A contains S. snyderi, S. mohavensis and S. obesus. B contains outgroups, Epizon alvordensis, S. isolatus, S. newarkensis, S. thalassinus, and S. bicolor. China Lake Naval Air Station, San Bernadino Co., CA Camp Cady Wildlife Area, San Bernadino Co., CA Walker Lake, Mineral Co., NV Walker Lake, Mineral Co., NV Humboldt River, Pershing Co., NV Little Soda Lake, Churchill Co., NV Pleasant Valley Spring, Pershing Co., NV Southwest Pond, Owens Valley, Inyo Co., CA Southwest Pond, Owens Valley, Inyo Co., CA Little Soda Lake, Churchill Co., NV Fish Lake Valley, Esmeralda Co., NV Fish Lake Valley, Esmeralda Co., NV Fish Lake Valley, Esmeralda Co., NV South Fork Humboldt River, Elko Co., NV Toulon Drain, Churchill Co., NV Owens River, Inyo Co., CA Northeast Pond, Owens Valley, Inyo Co., CA Northeast Pond, Owens Valley, Inyo Co., CA Northeast Pond, Owens Valley, Inyo Co., CA Cottonwood Pond, Owens Valley, Inyo Co., CA Cottonwood Pond, Owens Valley, Inyo Co., CA Big Smoky Valley, Nye Co., NV Big Smoky Valley, Nye Co., NV Big Smoky Valley, Nye Co., NV Pyramid Lake, Washoe Co., NV Humboldt River, Pershing Co., NV South Fork Reservoir, South Fork of Humboldt River, Elko Co., NV Pyramid Lake, Washoe Co., NV Flowing Wells, Railroad Valley, Nye Co., NV Hot Creek Valley, Nye Co., NV Bull Creek, Railroad Valley, Nye Co., NV Bull Creek, Railroad Valley, Nye Co., NV Little Fish Lake Valley, Nye Co., NV Blue Eagle Springs, Railroad Valley, Nye Co., NV Green Springs, Railroad Valley, Nye Co., NV Flowing Wells, Railroad Valley, Nye Co., NV Hot Creek Valley, Nye Co., NV Green Springs, Railroad Valley, Nye Co., NV Bull Creek, Railroad Valley, Nye Co., NV Little Fish Lake Valley, Nye Co., NV Pyramid Lake, Washoe Co., NV Kate Springs, Railroad Valley, Nye Co., NV Blue Eagle Springs, Railroad Valley, Nye Co., NV Blue Eagle Springs, Railroad Valley, Nye Co., NV Blue Eagle Springs, Railroad Valley, Nye Co., NV XL Springs, Abert Lake Basin, Lake Co., OR XL Springs, Abert Lake Basin, Lake Co., OR XL Springs, Abert Lake Basin, Lake Co., OR Brittain Springs, Abert Lake Basin, Lake Co., OR Hutton Springs, Alkali Lake Basin, Lake Co., OR Hutton Springs, Alkali Lake Basin, Lake Co., OR Crooked Creek, Abert Lake Basin, Lake Co., OR Crooked Creek, Abert Lake Basin, Lake Co., OR Brittain Springs, Abert Lake Basin, Lake Co., OR Casey Pond, Churchill Co., NV Casey Pond, Churchill Co., NV Eagle Lake, Lassen Co., CA Pyramid Lake, Washoe Co., NV Crooked Creek, Abert Lake Basin, Lake Co., OR Co. Road 4−17 Springs, Summer Lake Basin, Lake Co., OR An[n]a Reservoir, Lake Co., OR Co. Road 4−17 culvert crossing, Lake Co., OR Klippel Springs, Summer Lake Basin, Lake Co., OR Klippel Springs, Summer Lake Basin, Lake Co., OR Ana Reservoir, Summer Lake Basin, Lake Co., OR Ana Reservoir, Summer Lake Basin, Lake Co., OR Klippel Springs, Summer Lake Basin, Lake Co., OR Rickert Springs, Summer Lake Basin, Lake Co., OR Co. Road 4−17 Springs, Summer Lake Basin, Lake Co., OR Warm Springs Ranch, Elko Co., NV Warm Springs Ranch, Elko Co., NV Independence Valley, Elko Co., NV Fish Creek Springs, Eureka Co., NV Circle Ranch, White Pine Co., NV Newark Valley, White Pine Co., NV Newark Valley, White Pine Co., NV Little Smoky Valley, Eureka Co., NV Fish Creek Springs, Eureka Co., NV Circle Ranch, White Pine Co., NV Little Smoky Valley, Eureka Co., NV Newark Valley, White Pine Co., NV Three Mile Reservoir, Catlow Valley, Harney Co., OR Three Mile Reservoir, Catlow Valley, Harney Co., OR Roaring Springs, Catlow Valley, Harney Co., OR Roaring Springs, Catlow Valley, Harney Co., OR Roaring Springs, Catlow Valley, Harney Co., OR Skull Creek, Catlow Valley, Harney Co., OR Fish Creek, Guano Valley, Washoe Co., NV Three Mile Reservoir, Catlow Valley, Harney Co., OR Rock Creek, Catlow Valley, Harney Co., OR Rock Creek, Catlow Valley, Harney Co., OR Rock Creek, Catlow Valley, Harney Co., OR Silver Creek, Harney Co., OR Silver Creek, Harney Co., OR Silver Creek, Harney Co., OR Buck Creek, Silver Lake Valley, Lake Co., OR Buck Creek, Silver Lake Valley, Lake Co., OR Upper Klamath Lake, Klamath Co., OR Upper Klamath Lake, Klamath Co., OR Goose Lake, Lake Co., OR Goose Lake, Lake Co., OR Rickert Springs, Summer Lake Basin, Lake Co., OR Rickert Springs, Summer Lake Basin, Lake Co., OR Co. Road 4−17 Springs, Summer Lake Basin, Lake Co., OR Hart Lake, Warner Valley, Lake Co., OR Hart Lake, Warner Valley, Lake Co., OR Cowhead Slough, Cowhead Lake Valley, Modoc Co., CA OR002152.1 JX443069.1 NC_066714.1 MN296123.1 AF370041.1 OL806603.1 NC_060660.1 JX443074.1 JX443073.1 AF370042.1 JX443071.1 AF370118.1 JX443090.1 JX443033.1 JX443030.1 AF370115.1 AF370116.1 AF370117.1 JX443005.1 JX443003.1 AF370043.1 MF972070.1 AF370044.1 AF370045.1 AF370046.1 AF237737.1 AF370048.1 SWP22_13 SWP22_15 AF237736.1 AF370057.1 AF370058.1 AF237751.1 AF237752.1 AF237753.1 AF370056.1 NEP17_01 NEP17_03 NEP17_04 CWP10_56 CWP10_58 AF370064.1 AF237738.1 AF237739.1 AF370053.1 AF370047.1 JX443080.1 AF370052.1 AF237743.1 AF237742.1 AF370059.1 AF237747.1 AF237740.1 AF237749.1 AF237744.1 AF237748.1 AF237741.1 AF370062.1 AF237750.1 AF370063.1 AF370054.1 AF370060.1 AF370061.1 AF237745.1 AF237746.1 AF370065.1 AF370066.1 AF370067.1 AF370068.1 AF370070.1 AF370071.1 AF370072.1 AF370074.1 AF370069.1 AF370049.1 AF370050.1 AF370051.1 AF370055.1 AF370073.1 AF370076.1 OL457405.1 OL457402.1 AF370079.1 AF370078.1 AF370081.1 AF370082.1 AF370080.1 AF370075.1 AF370077.1 AF370083.1 AF370084.1 AF237760.1 AF370085.1 AF370088.1 AF237755.1 AF237757.1 AF237756.1 AF370086.1 AF370087.1 AF237758.1 AF237754.1 AF370089.1 AF370090.1 AF370097.1 AF370096.1 AF370098.1 AF370095.1 AF370099.1 AF370091.1 AF370092.1 AF370093.1 AF370094.1 AF370100.1 AF370101.1 AF370102.1 AF370103.1 AF370104.1 AF370105.1 AF370106.1 AF370107.1 AF370108.1 AF370109.1 AF370110.1 AF370111.1 AF370112.1 AF370113.1 AF370114.1 S. obesus mt lineage S. bicolor mt lineage S. thalassinus mt lineage S. newarkensis mt lineage S. isolatus mt lineage S. mohavensis mt lineage Figure S1 Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Mule Spring Owens River Cottonwood Pond (White MT Research Center) Owens River Mule Spring Owens River Mule Spring Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Mule Spring Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Cottonwood Pond (White MT Research Center) Owens River Mule Spring Owens River Mule Spring Owens River Mule Spring Owens River Mule Spring Owens River Cottonwood Pond (White MT Research Center) Owens River Mule Spring Owens River Mule Spring Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River SW Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River SW Pond (White Mountain Research Center) Owens River SE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River NE Pond (White Mountain Research Center) Owens River SE Pond (White Mountain Research Center) Mammoth Mountain Sotcher Lake Outlet, Reds Meadow Creek Mammoth Mountain Sotcher Lake Mammoth Mountain Sotcher Lake Mammoth Mountain Sotcher Lake Mammoth Mountain Sotcher Lake Mammoth Mountain Sotcher Lake Mammoth Mountain Sotcher Lake Mammoth Mountain Sotcher Lake Mammoth Mountain Sotcher Lake Mammoth Mountain Sotcher Lake Mammoth Mountain Sotcher Lake Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Drainage Tui Slough Mojave River Camp Cady Mojave River Camp Cady Mojave River Camp Cady Mojave River Camp Cady Mojave River Camp Cady Mojave River Camp Cady Mojave River Camp Cady Mojave River Camp Cady Mojave River Camp Cady Mojave River Camp Cady Mojave River Camp Cady Mojave River Camp Cady Mojave River Camp Cady Mojave River Camp Cady Mojave River Camp Cady Mojave River Camp Cady Mojave River Drainage Tui Slough Little Fish Lake Valley Little Fish Lake Little Fish Lake Valley Little Fish Lake Little Fish Lake Valley Little Fish Lake Little Fish Lake Valley Little Fish Lake Little Fish Lake Valley Little Fish Lake Little Fish Lake Valley Little Fish Lake Railroad Valley Flowing Wells Railroad Valley Flowing Wells Railroad Valley Flowing Wells Hot Creek Valley Twin Springs Slough Railroad Valley Flowing Wells Railroad Valley Flowing Wells Railroad Valley Flowing Wells Railroad Valley Flowing Wells Railroad Valley Flowing Wells Railroad Valley Flowing Wells Railroad Valley Flowing Wells Railroad Valley Flowing Wells Railroad Valley Flowing Wells Railroad Valley Flowing Wells Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Railroad Valley Flowing Well #7 Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Railroad Valley Flowing Wells Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Hot Creek Valley Twin Springs Slough Fish Lake Valley McNett Ranch Fish Lake Valley McNett Ranch Fish Lake Valley McNett Ranch Fish Lake Valley McNett Ranch Fish Lake Valley McNett Ranch Fish Lake Valley McNett Ranch Fish Lake Valley Lida Pond Fish Lake Valley Lida Pond Fish Lake Valley Lida Pond Fish Lake Valley Lida Pond Fish Lake Valley Lida Pond Fish Lake Valley Lida Pond Fish Lake Valley Lida Pond Fish Lake Valley Lida Pond Fish Lake Valley Lida Pond Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Humboldt River System Upper Humboldt River Walker Lake Rose Creek Reservoir Walker Lake Rose Creek Reservoir Walker Lake Rose Creek Reservoir Walker Lake Rose Creek Reservoir Walker Lake Rose Creek Reservoir Walker Lake Rose Creek Reservoir Walker Lake Rose Creek Reservoir Walker Lake Rose Creek Reservoir Walker Lake Rose Creek Reservoir Walker Lake Rose Creek Reservoir Walker Lake Rose Creek Reservoir Walker Lake Rose Creek Reservoir Walker Lake Rose Creek Reservoir Walker Lake Rose Creek Reservoir Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Carson River, Carson Desert Little Soda Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake Lassen County Eagle Lake To Figure S2B A S. mohavensis S. snyderi ‘toikona’ S. snyderi S. obesus Figure S2 Unknown Unknown Unknown Unknown Unknown Unknown Unknown Unknown Unknown Mojave River Drainage Desert Discovery Pond Mojave River Drainage Desert Discovery Pond Sheldon Wildlife Refuge Thousand Creek Gorge Sheldon Wildlife Refuge Thousand Creek Gorge Sheldon Wildlife Refuge Thousand Creek Gorge Sheldon Wildlife Refuge Thousand Creek Gorge Sheldon Wildlife Refuge Thousand Creek Gorge Sheldon Wildlife Refuge Thousand Creek Gorge Sheldon Wildlife Refuge Thousand Creek Gorge Sheldon Wildlife Refuge Thousand Creek Gorge Sheldon Wildlife Refuge Thousand Creek Gorge Sheldon Wildlife Refuge Thousand Creek Gorge Valley Falls Honey Creek Valley Falls Honey Creek Valley Falls Honey Creek Valley Falls Honey Creek Valley Falls Honey Creek Valley Falls Honey Creek Valley Falls Honey Creek Valley Falls Honey Creek Pit River Big Sage Reservoir Pit River Big Sage Reservoir Pit River Big Sage Reservoir Pit River Big Sage Reservoir Pit River Big Sage Reservoir Pit River Big Sage Reservoir Pit River Big Sage Reservoir Pit River Big Sage Reservoir Pit River Big Sage Reservoir Pit River Big Sage Reservoir Pit River Big Sage Reservoir Pit River Big Sage Reservoir Warner Spring Twenty Mile Slough Warner Spring Twenty Mile Slough Warner Spring Twenty Mile Slough Sheldon Wildlife Refuge Bitner Ranch Sheldon Wildlife Refuge Bitner Ranch Sheldon Wildlife Refuge Bitner Ranch Sheldon Wildlife Refuge Bitner Ranch Sheldon Wildlife Refuge Bitner Ranch Sheldon Wildlife Refuge Andy's Place Sheldon Wildlife Refuge Bitner Ranch Sheldon Wildlife Refuge Bitner Ranch Sheldon Wildlife Refuge Bitner Ranch Sheldon Wildlife Refuge Andy's Place Sheldon Wildlife Refuge Horse Canyon Sheldon Wildlife Refuge Horse Canyon Sheldon Wildlife Refuge Andy's Place Sheldon Wildlife Refuge Andy's Place Sheldon Wildlife Refuge Andy's Place Sheldon Wildlife Refuge Andy's Place Sheldon Wildlife Refuge Andy's Place Sheldon Wildlife Refuge Andy's Place Sheldon Wildlife Refuge Horse Canyon Sheldon Wildlife Refuge Horse Canyon Sheldon Wildlife Refuge Andy's Place Sheldon Wildlife Refuge Andy's Place Sheldon Wildlife Refuge Bitner Ranch Sheldon Wildlife Refuge Andy's Place Sheldon Wildlife Refuge Andy's Place Sheldon Wildlife Refuge Andy's Place Sheldon Wildlife Refuge Andy's Place Sheldon Wildlife Refuge Andy's Place Sheldon Wildlife Refuge Bitner Ranch Sheldon Wildlife Refuge Bitner Ranch Sheldon Wildlife Refuge Bitner Ranch Sheldon Wildlife Refuge Bitner Ranch Sheldon Wildlife Refuge Bitner Ranch Sheldon Wildlife Refuge Bitner Ranch Sheldon Wildlife Refuge Bitner Ranch Catlow Valley Rock Creek Catlow Valley Rock Creek Catlow Valley Rock Creek Harney County Kueny Canyon Harney County Kueny Canyon Harney County Kueny Canyon Harney County Kueny Canyon Harney County Kueny Canyon Harney County Kueny Canyon Harney County Kueny Canyon Harney County Kueny Canyon Harney County Kueny Canyon Harney County Kueny Canyon Harney County Kueny Canyon Modoc County Cowhead Slough Modoc County Cowhead Slough Modoc County Cowhead Slough Modoc County Cowhead Slough Modoc County Cowhead Slough Modoc County Cowhead Slough Modoc County Cowhead Slough Modoc County Cowhead Slough Modoc County Cowhead Slough Modoc County Cowhead Slough Modoc County Cowhead Slough Modoc County Cowhead Slough Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NV12 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Newark Valley NN4 Independence Valley Warm Springs Independence Valley Warm Springs Independence Valley Warm Springs Independence Valley Warm Springs Independence Valley Warm Springs Independence Valley Warm Springs Independence Valley Warm Springs Independence Valley Warm Springs Independence Valley Warm Springs Independence Valley Warm Springs Independence Valley Warm Springs Independence Valley Warm Springs Independence Valley Warm Springs Independence Valley Warm Springs Owens River Cottonwood Pond (White MT Research Center) Owens River Mule Spring 0.03 S. bicolor S. thalassinus Epizon alvordensis Pogonichthys macrolepidotus Gila orcuttii S. newarkensis S. isolatus To Figure S2A B Figure S2