A new subspecies of Corydalis wendelboi Lidén (Papaveraceae) from NW Türkiye, evidence from morphological and molecular analysis

2025  47  21 adansonia Adansonia est une revue en flux continu publiée par les Publications scientifiques du Muséum, Paris Adansonia is a fast track journal published by the Museum Science Press, Paris Les Publications scientifiques du Muséum publient aussi / The Museum Science Press also publish: Geodiversitas, Zoosystema, Anthropozoologica, European Journal of Taxonomy, Naturae, Cryptogamie sous-sections Algologie, Bryologie, Mycologie, Comptes Rendus Palevol Diffusion – Publications scientifiques Muséum national d’Histoire naturelle CP 41 – 57 rue Cuvier F-75231 Paris cedex 05 (France) Tél. : 33 (0)1 40 79 48 05 / Fax : 33 (0)1 40 79 38 40 [email protected] / http://sciencepress.mnhn.fr © Publications scientifiques du Muséum national d’Histoire naturelle, Paris, 2025 ISSN (imprimé / print) : 1280-8571/ ISSN (électronique / electronic) : 1639-4798 Directeur De la publication / Publication director: Gilles Bloch Président du Muséum national d’Histoire naturelle réDacteur en chef / editor-in-chief : Thierry Deroin réDacteurs / editors : Porter P. Lowry II ; Zachary S. Rogers ; Mathieu Gardère assistant De réDaction / assistant editor : Emmanuel Côtez ([email protected]) Mise en page / Page layout : Emmanuel Côtez coMité scientifique / scientific board : F. Blasco (CNRS, Toulouse) M. W. Callmander (Conservatoire et Jardin botaniques de Genève) J. A. Doyle (University of California, Davis) P. K. Endress (Institute of Systematic Botany, Zürich) P. Feldmann (Cirad, Montpellier) L. Gautier (Conservatoire et Jardin botaniques de Genève) F. Ghahremaninejad (Kharazmi University, Téhéran) K. Iwatsuki (Museum of Nature and Human Activities, Hyogo) A. A. Khapugin (Tyumen State University, Russia) J.-Y. Lesouef (Conservatoire botanique de Brest) P. Morat (Muséum national d’Histoire naturelle, Paris) J. Munzinger (Institut de Recherche pour le Développement, Montpellier) S. E. Rakotoarisoa (Millenium Seed Bank, Royal Botanic Gardens Kew, Madagascar Conservation Centre, Antananarivo) P. H. Raven (Missouri Botanical Garden, St. Louis) G. Tohmé (Conseil national de la Recherche scientifique Liban, Beyrouth) J. G. West (Australian National Herbarium, Canberra) J. R. Wood (Oxford) couverture / cover : Réalisée à partir des Figures de l’article/Made from the Figures of the article. Adansonia est indexé dans / Adansonia is indexed in: – Science Citation Index Expanded (SciSearch®) – ISI Alerting Services® – Current Contents® / Agriculture, Biology, and Environmental Sciences® – Scopus® Adansonia est distribué en version électronique par / Adansonia is distributed electronically by: – BioOne® (http://www.bioone.org) 341 ADANSONIA, sér. 3 • 2025 • 47 (21) © Publications scientifiques du Muséum national d’Histoire naturelle, Paris. www.adansonia.com KEY WORDS Papaveraceae, Corydalis, Turkey, ITS, identification key, new subspecies. MOTS CLÉS Papaveraceae, Corydalis, Turquie, ITS, clé d’identification, sous-espèce nouvelle. Bülent OLCAY Department of Pharmaceutical Botany, Faculty of Pharmacy, Istanbul University, Istanbul (Turkey) corresponding author ([email protected]) S� ükranKÜLTÜR Department of Pharmaceutical Botany, Faculty of Pharmacy, Istanbul University, Istanbul (Turkey) AslıDOG �RU-KOCA Department of Biology, Faculty of Science, Hacettepe University, Ankara (Turkey) Submitted on 28 February 2025 | accepted on 30 April 2025 | published on 24 November 2025 A new subspecies of Corydalis wendelboi Lidén (Papaveraceae)fromNWTürkiye,evidence frommorphologicalandmolecularanalysis Olcay B., Kültür Ş.& Doğru-Koca A. 2025. — A new subspecies of Corydalis wendelboi Lidén (Papaveraceae) from NW Türkiye, evidence from morphological and molecular analysis. Adansonia, sér. 3, 47 (21): 341-351. https://doi.org/10.5252/adansonia2025v47a21. http://adansonia.com/47/21 ABSTRACT Corydalis wendelboi subsp. fragilis subsp. nov. (Papaveraceae), a new subspecies is described from northwest Anatolia, Türkiye. It is closely related to C. wendelboi subsp. wendelboi Lidén and C. wendelboi subsp. congesta Lidén& Zetterl. but it differs in having very narrowly lobed leaves, in having bracts, which are smaller and divided into very thin lobes, and in more lax racemes. The ribosomal internal transcribed spacer (ITS 1, 5.8 S rRNA, ITS 2) region was employed to ascertain the phylogenetic relationship of Corydalis DC. species, utilising Maximum Likelihood and Bayes Inference methods. The phylogenetic results provided compelling evidence in support of the description of this new subspecies. An identification key has been created to facilitate the morphological distinction of closely related taxa. RÉSUMÉ Une sous-espèce nouvelle de Corydalis wendelboi Lidén (Papaveraceae) du nord-ouest de la Turquie, appuyée par des analyses morphologiques et moléculaires. Corydalis wendelboi subsp. fragilis subsp. nov. (Papaveraceae), une sous-espèce nouvelle est décrite du nord-ouest de l’Anatolie, en Turquie. Elle est étroitement apparentée à C. wendelboi subsp. wendelboi Lidén et à C.wendelboi subsp. congesta Lidén& Zetterl., mais elle s’en distingue par ses feuilles très étroitement lobées, ses bractées plus petites et divisées en lobes très fins, et ses grappes plus lâches. La région de l’espace interne transcrit ribosomique (ITS 1, 5.8 S rRNA, ITS 2) a été utilisée pour déterminer la relation phylogénétique des espèces Corydalis DC., en utilisant les méthodes de maximum de vraisemblance et d’inférence bayésienne. Les résultats phylogénétiques ont fourni des preuves convaincantes, soutenant la description de cette nouvelle sous-espèce. Une clé d’identification est fournie, pour faciliter la distinction morphologique des taxons étroitement apparentés. 342 ADANSONIA, sér. 3 • 2025 • 47 (21) Olcay B. et al. INTRODUCTION Corydalis DC. in Lamarck& Candolle (1805) is the largest genus in the family Papaveraceae, and comprises about 538 species (POWO 2024). Corydalis species are distributed in temperate regions of the Northern Hemisphere (Lidén& Zetterlund 1997). The genus Corydalis has consistently been regarded as one of the most taxonomically challenging due to species identification and complex polymorphisms. In recent years, extensive phylogenetic studies have enabled Corydalis to be classified with greater accuracy (Pérez-Gutiérrez etal. 2015; Ren etal. 2019; Xu& Wang 2021; Xu etal. 2022; Chen etal. 2023; Liu etal. 2024). It has been proposed that morphological data should be interpreted in conjunction with molecular data in the infrageneric classification of Corydalis and that the difficulties encountered in the classification of species exhibiting variation can be illuminated by molecular data (Pérez-Gutiérrez etal. 2015; Ren etal. 2019). The genus Corydalis is represented by 23 taxa (18 species and five subspecies) in Türkiye, including the recently published Corydalis varolaydinii Yıldırım. Of these 23 taxa, nine are endemic (Aslan 2012; Özdöl etal. 2024). The first comprehensive revision of the genus Corydalis in Türkiye was made by Davis& Cullen (1965) in Flora of Turkey and the East Aegean Islands. Later in volume 11 of this work, published in 2000, the genus Corydalis was revised again by Lidén (2000). He described some new species, synonymized some taxa published in the first volume of Flora of Turkey and the East Aegean Islands, and discussed the current taxonomic status of these taxa. Lidén (1988) described a new species, Corydalis wendelboi Lidén& Zetterl, from Muğla province in southwestern Türkiye. He reported that this species was distributed in western Anatolia and had previously been described as C. solida subsp. solida (L.) Clairv. by Davis& Cullen (1965) in the first volume of the Flora of Turkey. In later work, Lidén (1996) described the new subspecies C. wendelboi subsp. congesta Lidén& Zetterl. from Uludağ in northwestern Anatolia. He reported that this taxon is homogeneous in the whole area of its distribution, but in the province of Ankara, there are also forms with narrower leaf lobes. Additionally, Lidén elevated C. solida subsp. tauricola to the category of species. The Corydalis specimens were collected as part of the first author’s PhD thesis. Molecular studies as part of this thesis showed that some populations previously identified as C.wendelboi formed a separate lineage. A larger sample was then examined morphologically. This examination led to the conclusion that some populations represented a new subspecies. MATERIAL AND METHODS Plant material and morPhological studies The first author collected Plant samples in Türkiye between 2019 and 2024. The specimens were identified and their morphological characteristics were analyzed in detail. Plant samples were identified using Flora of Turkey and the East Aegean Islands Vol 11 (Lidén 2000). Prepared voucher specimens were deposited in the Herbarium of the Faculty of Pharmacy, Istanbul University (ISTE). Many Corydalis specimens in other herbaria, such as ANK, GAZI, HUB, ISTF, and others in Türkiye were also investigated. The complete list of specimens examined is given in the Appendix. dna extraction, Pcr and sequencing The total genomic DNAs were isolated from silica gel dried leaf tissues from Corydalis wendelboi subsp. fragilis subsp.nov., C. wendelboi subsp. wendelboi and C. wendelboi subsp. congesta using Exgene™ Plant SV GeneAll® Mini Kit (Seoul, Republic of Korea). The DNA extraction protocol indicated by the manufacturer was followed. The primer pair used for amplifying the ITS region was ITS5/ITS4 (White etal. 1990). The relevant marker sequences of all of the other taxa used for phylogenetic analysis were obtained from GenBank. Their NCBI accession numbers are listed in Table 1. In the present study, the ITS1, 5.8S rRNA, ITS2 region was amplified in a single reaction using ITS 4 (R) (5’ TCC TCC GCT TAT TGA TAT GC 3’) and ITS 5 (F) (5’ GGA AGT AAA AGT CGT AAC AAG G 3’) primers, following White etal. (1990). The thermocycling conditions were as follows: initial denaturation step of 94°C for 3min, followed by 35 cycles: denaturation at 94°C for 60s, primer annealing at 53°C for 90s, and extension at 72°C for 90s; a final extension at 72°C for 10min; and maintenance at 4°C before electrophoresis. PCR reactions were carried out using the T100 Thermal Cycler (Bio-Rad, Hercules, CA, United States). Sanger sequencing of PCR products was performed by BM Labosis Inc (Ankara, Türkiye). Phylogenetic analyses In the present study, sequences of ribosomal DNA internal transcribed spacer (ITS1, 5.8S rRNA, ITS2) were obtained from three samples belonging to two different populations of C. wendelboi subsp. fragilis subsp.nov. and from samples belonging to one population, each from five different Corydalis taxa distributed in Türkiye and closely related to the new subspecies. In addition to the ITS sequences of Corydalis taxa obtained in this study, the datasets of a total of 26 Corydalis taxa, the majority of which are not distributed in Türkiye, were obtained from the NCBI database and included in the study. All sequences found for the sect. Corydalis (with the exception of duplicates and those of doubtful identification) has been added to the phylogenetic tree. Corydalis bungeana Turcz. was selected as an outgroup (Jiang etal. 2018; Ren etal. 2019; Xu etal. 2022). The data sets taken from the NCBI database and used in the analyses are given in Table 1 with their accession numbers. All sequences were aligned with MAFFT v.7 (Katoh etal. 2017) and then manually checked with BioEdit v.7.2. The evolutionary substitution model was determined as GTRGamma according to Akaike Information Criterion(AIC) using RaxMLGUI 2.0. Maximum Likelihood (ML) analyses of the aligned data were performed using RaxMLGUI 2.0 (Edler 343 A new subspecies of Corydalis wendelboi Lidén from NW Türkiye (Papaveraceae) ADANSONIA, sér. 3 • 2025 • 47 (21) etal. 2021). In 500 simultaneously conducted analyses, the ‘Bootstrap’ analysis was performed with 1000 replications. Bayesian analysis (BI) was performed at 10× 106 generations using BEAUti and BEAST 2.7.4 programs. The maximum clade credibility (MCC) tree was constructed using TreeAnnotator 2.7.4 (Drummond& Rambaut 2007). The resulting trees were visualized and edited in FigTree v1.4.4. (Rambaut 2018). The first 10% of the resulting trees were burned. Log files were checked in Tracer v.1.7.1 (Rambaut etal. 2018). Effective sample size (ESS) values were greater than 200 for each run. Posterior probabilities (PP) and bootstrap support values (BS) were added to the phylogenetic tree (Figure 1). abbreviations Herbarium acronyms AEF Herbarium, Faculty of Pharmacy, Ankara University; ANK Herbarium, Faculty of Science, Ankara University DUOF Herbarium of Duzce University Faculty of Forestry E Herbarium, Royal Botanic Garden Edinburgh; EGE Herbarium, Faculty of Science, Ege University; GAZI Herbarium, Faculty of Science, Gazi University, Ankara; HUB Herbarium, Faculty of Science, Hacettepe University, Ankara; ISTE Herbarium, Faculty of Pharmacy, Istanbul University; ISTF Herbarium, Faculty of Science, Istanbul University; NGBB Herbarium, Nezahat Gökyiğit Botanical Garden, Istanbul. TAXONOMIC TREATMENT Family PaPaveraceae Juss. Genus Corydalis DC. Corydalis wendelboi subsp. fragilis Olcay, Doğru-Koca& Kültür, subsp.nov. (Figs 2; 3) tyPe material. — Turkey • A3 Ankara, Kızılcahamam, Şahinler Nature Park, woodlands; 40°38’10.2”N, 32°26’10.3”E; 1480m a.s.l.; 7.V.2021; B.Olcay s.n.; holotype: ISTE[ISTE118601]!; isotype: HUB!• A3 Bolu, Near Aladağ Lake; 1391m a.s.l.; 7.V.2021; B.Olcay s.n.; paratype: ISTE[ISTE118598]!). diagnosis. — Corydalis wendelboi subsp. fragilis subsp.nov. is closely related to C. wendelboi subsp. wendelboi, C. wendelboi subsp. congesta and C. tauricola, because of its divided bracts; a ratio of pedicel to bract length and obtuse lower petals. However, it differs from C. tauricola by its short pedicels, 1-2mm (not 7-15mm) and its slightly incised ± equal lobes of bracts (not central lobe of bract largest). It differs from C. wendelboi taxa by its narrowly lobed leaves, 2-3mm broad (not 3.55mm); by its smaller bracts, 5-6mm long (not 9-10mm), which are divided into very thin lobes (not divided into thick lobes as in subsp. wendelboi or into moderately thin lobes as in subsp. congesta) and by its lax racemes (not dense). C. wendelboi subsp. fragilis subsp.nov. also differs from subsp. wendelboi because of its smaller seeds, 1-1.2mm in diameter (c.1.5mm in diameter in subsp. wendelboi) (Table 2; Figs 3; 4). table 1. — Taxa, voucher numbers/reference and accession numbers of ITS sequences utilized in this study. The bold accession numbers are obtained herein. Taxon Voucherno./reference Accessionnumber Corydalis wendelboi subsp. fragilis subsp. nov. ISTE118601 (holotype) PP849377 Corydalis wendelboi subsp. fragilis subsp. nov. ISTE 18598 PP849378 Corydalis wendelboi subsp. fragilis subsp. nov. ISTE118781 PQ656721 Corydalis wendelboi subsp. congesta Lidén & Zetterl. ISTE118591 PP849382 Corydalis wendelboi subsp. wendelboi Lidén & Zetterl. ISTE118780 PQ656720 Corydalis wendelboi subsp. wendelboi Lidén & Zetterl. ISTE118585 PP849381 Corydalis tauricola (Cullen & Davis) Lidén ISTE94344 PP849383 Corydalis “caucasica alba”Lidén et al. 1995 X85468 Corydalis intermedia Mérat V-92966 MN662998 Corydalis henrikii Lidén Lidén et al. 1995 X85476, X85487 Corydalis solida subsp. incisa Lidén ISTE117077 PP849380 Corydalis solida subsp. solida (L.) Clairv. ISTE117072 PP849379 Corydalis pumila W.D.J. Koch O-392657 MN662999 Corydalis solida subsp. solida (L.) Clairv. Lidén et al. 1995 X85470, X85472 Corydalis solida subsp. incisa Lidén Lidén et al. 1995 X85464, X85471 Corydalis decumbens Pers. ZJ006MT01 MH349117 Corydalis decumbens Pers. ZJ006MT03 MH349119 Corydalis tauricola (Cullen & Davis) Lidén Lidén et al. 1995 X85478, X85480 Corydalis repens var. repens Mandl & Muehld. ZJ013MT02 MH349154 Corydalis repens var. repens Mandl & Muehld. ZJ013MT01 MH349153 Corydalis repens var. watanabei (Kitag.) Y.C.Chu ZJ014MT01 MH349156 Corydalis repens var. watanabei (Kitag.) Y.C.Chu ZJ014MT02 MH349157 Corydalis repens var. repens Mandl & Muehld. ZJ013MT03 MH349155 Corydalis ambigua Cham. & Schltdl. –FJ469598 Corydalis linjiangensis Z.Y.Su ex Lidén ZJ009MT02 MH349137 Corydalis linjiangensis Z.Y.Su ex Lidén ZJ009MT03 MH349138 Corydalis turtschaninovii Besser ZJ020MT04 MH349205 Corydalis turtschaninovii Besser NICOMVP0000 KY305172 Corydalis yanhusuo W.T.Wang ZJ022MT06 MH349215 Corydalis yanhusuo W.T.Wang ZJ022MT01 MH349210 Corydalis dajingensis C.Y.Wu & Z.Y.Su 39676 MH293121 Corydalis benecincta W.W.Sm. ZYZ90 MH293114 Corydalis glaucescens Regel Q-010 MT924005 Corydalis nariniana Fed. Lidén et al. 1995 X85460, X85463 Corydalis bungeana Turcz. Ren et al. 2019 MH349108 344 ADANSONIA, sér. 3 • 2025 • 47 (21) Olcay B. et al. e tymology . — The epithet ‘fragilis’ alludes to the delicate and fragile nature of the plant. Phenology. — Flowering commences at the beginning of April and continues until the end of May. Mature fruits have been collected in May. distribution and ecology. — C. wendelboi subsp. fragilis subsp.nov. is endemic to Türkiye and is known from only two localities near the border between the provinces of Bolu and Ankara (Figs 5; 6). It occurs in the subalpine zone, at altitudes of 1400-1600m a.s.l, in rocky habitats in sparse Pinus nigra forests and areas sheltered by Juniperus communis subsp. nana. iucn status. — C. wendelboi subsp. fragilis subsp.nov. is known from only two locations in Bolu and Ankara provinces (Criterion B2a), with an estimated area of occupancy that is less than 10km2 (Criterion B2b). A small population of between 30 and 40 individuals has been observed at both locations. Furthermore, no subpopulation was found to contain more than 50mature individuals, with the expected continuing decline observed in all cases (Criterion C2). The number of mature individuals is less than 50 (Criterion D). Furthermore, the species’ habitat has been affected by factors such as road construction, overgrazing and human pressure. Therefore, it should be classified as “Critically Endangered (CR)” according to the IUCN Red List criteria (IUCN 2022). Category: CR. B2ab(ii, iv, v); C2a(i); D. local name.— The Turkish name “Narin tarlakuşu” is proposed for this subspecies, according to the guidelines of Menemen etal. (2016). additional sPecimens examined. — C. wendelboi subsp. fragilis subsp.nov.: Turkey• A3 Ankara, Kızılcahamam, Şahinler Nature Park, woodlands; 40°38’10.2”N, 32°26’10.3”E; 1450m a.s.l.; 22.IV.2024; B. Olcay& F. Nur Olcay s.n.; ISTE[ISTE118781]!). C.wendelboi subsp. wendelboi: Turkey• C2 Denizli, Mount Honaz, Tuzluca Plateau; 1320m a.s.l.; 8.IV.1973; E. Tuzlacı s.n.; ISTE[ISTE24024]! • C2 Muğla, Muğla-Göktepe TV transmitting station; 1450m a.s.l.; 20.IV.1981; M. A. Önür 483; ISTE[ISTE47753]!• C4 Konya, Ermenek; 1.IV.1993; A. Byfield 151; ISTE[ISTE65756]!• C3 Antalya, Elmalı, Çam Kuyusu district; 1580m a.s.l.; 16.IV.1993; N.Özhatay& A. Byfield s.n.; ISTE[ISTE65037] • B3 Afyon, Sultandağları, above Dereçine; 1450m a.s.l.; 19 IV.1976; A. Baytop s.n.; ISTE[ISTE34558]! • B1 İzmir, Mount Nif; 990m a.s.l.; 26.III.1981; A.&T. Baytop s.n.; ISTE[ISTE46151]! • C4 Konya, Ermenek, Mount Yeşilgöl; 1500m a.s.l.; Remark& Wendelbo s.n.; ISTE [ISTE101596]! • C3 Konya, Beyşehir, Kurucova, Suludere district; 1500m a.s.l.; 8.VI.1975; H. Peşmen& A. Güner 2183; HUB[HUB01517]! • C4 Konya, Seydişehir, Şahinkayası district; 1500m a.s.l.; 14.IV.1982; H. Ocakverdi s.n.; ANK[ANK1358]!• C2 Muğla, Köyceğiz, Sultaniye district; 17.IV.1992; A. Güner H. Duman& A. Dönmez 10450; GAZI[GAZI 210919]! • A3 Ankara, Ayaşbeli Çal Hill; 1380m a.s.l.; 13.IV.1986; M. Vural s.n.; GAZI[GAZI4030]! • C3 Konya, Beyşehir-Akseki Plateau, Baharan district; 1400m a.s.l.; 21.IV.1983, M. Koyuncu 5934; AEF[AEF13415]! • A3 Bolu, Göynük, Sünnetgölü district; 965m a.s.l.; 7.IV.2009; A. Güner, M.Johnson& N. İkinci 15387; NGBB[NGBB001980]! • B4 Ankara, Beypazarı, Sığıryatağı Hill; 1400m a.s.l.; 24.IV.2013; A.Ö. Çimen s.n.; NGBB[NGBB009180]! • B2 İzmir, Ödemiş, above Bozdağ fig. 1. — Bayesian consensus tree of Corydalis DC. species based on ribosomal DNA internal transcribed spacer (ITS 1, 5.8S, ITS 2). The numbers adjacent to the nodes indicate posterior probabilities and bootstrap values obtained, respectively, derived from BI and ML analysis. CLADE A CLADE B CLADE C Out Group 1/35 0.9/68 0.97/90 1/100 1/70 1/99 1/91 1/100 1/100 0.83/76 1/85 1/71 1/99 1/93 0.88/63 0.86/63 0.99/73 0.97/73 0.76/ 74 1/-- 1/87 0.77/32 0.73/-- Corydalis wendelboi subsp. fragilis subsp. nov., PP849377, Türkiye (holotype) Corydalis wendelboi subsp. fragilis subsp. nov., PQ656721, Türkiye Corydalis wendelboi subsp. fragilis subsp. nov., PP849378, Türkiye Corydalis tauricola, PP849383, Türkiye Corydalis wendelboi subsp. wendelboi, PP849381, Türkiye Corydalis wendelboi subsp. wendelboi, PQ656720, Türkiye Corydalis wendelboi subsp. congesta, PP849382, Türkiye Corydalis henrikii, X85476-X85487, Türkiye Corydalis “caucasica alba”, X85468-X85469 Corydalis intermedia, MN662998, Norway Corydalis pumila, MN662999, Norway Corydalis solida subsp. solida, PP849379, Türkiye Corydalis solida subsp. incisa, PP849380, Türkiye Corydalis solida subsp. solida, X85470-X85472 Corydalis solida subsp. incisa, X85464-X85471, Greece Corydalis decumbens, MH349117, China Corydalis decumbens, MH349119, China Corydalis tauricola, X85478-X85480 Corydalis repens var. repens, MH349155 China Corydalis repens var. repens, MH349154 China Corydalis repens var. repens, MH349153 China Corydalis repens var. watanabei, MH349157 China Corydalis repens var. watanabei, MH349156 China Corydalis ambigua, FJ469598, China Corydalis linjiangensis, MH349138, China Corydalis linjiangensis, MH349137, China Corydalis turtschaninovii, KY305172, China Corydalis turtschaninovii, MH349205, China Corydalis yanhusuo, MH349215, China Corydalis yanhusuo, MH349210, China Corydalis dajingensis, MH393121, China Corydalis benecincta, MH393114, China Corydalis glaucescens, MT924005, China Corydalis nariniana, X85460-X85463, Türkiye Corydalis bungeana, MH349108, China 345 A new subspecies of Corydalis wendelboi Lidén from NW Türkiye (Papaveraceae) ADANSONIA, sér. 3 • 2025 • 47 (21) village; 1300m a.s.l.; 3.IV.1972; E. Leblebici s.n.; EGE[EGE15109]!. Corydalis wendelboi subsp. congesta: Turkey• A3 Bolu, around Lake Abant; 1350m a.s.l.; 28.IV.2019; M. Koçyiğit s.n.; ISTE[ISTE116560]!• A2 (A) Bursa, Uludağ, Kirazlıyayla district; 24.IV.1964; A& T. Baytop s.n.; ISTE[ISTE7587]! • B3 Eskişehir, between the junction of Kütahya and Eskişehir roads and Bozüyük; 17.IV.1965; A& T. Baytop s.n.; ISTE[ISTE7878]!• A5 Amasya, Doğantepe Kayacık village; 950m a.s.l.; 11.IV.1982; A& T. Baytop s.n.; ISTE[ISTE48459]!• A2 Kocaeli, AB C fig. 2. — Corydalis wendelboi subsp. fragilis subsp. nov.: A, habit; B, inflorescence; C, fruits and bracts. Scale bars: A, 3 cm; B, C, 1 cm. Photos by B. Olcay. table 2. — Morphological features comparisons of Corydalis wendelboi subsp. fragilis subsp. nov., C. wendelboi subsp. wendelboi Lidén, C. wendelboi subsp. congesta Lidén & Zetterl., and C. tauricola (Cullen & P.H.Davis) Lidén. C. wendelboisubsp. congesta C. wendelboisubsp. wendelboi C. wendelboisubsp.fragilis subsp.nov. C. tauricola Leaf lobes 4-5 mm wide 4-5 mm wide less than 3mm wide 4-5 mm wide Inflorescence very dense, 10-25 flowered moderately dense, 10-15 flowered lax, 5-15 flowered moderately dense, 5-15 flowered Bracts c. 10 mm long, broadly flabellate, divided into unequal sized lobes c. 10 mm long, broadly obovate, divided into ± equal sized lobes c. 5 mm long, obovate, deeply divided into dense, unequal sized lobes c. 10 mm long, obovate, divided, central lobe of bracts usually largest Pedicels 2-4 mm long 2-4 mm long 1-2 mm long 7-15 mm long Corolla lower petal 4-6 mm broad, not emarginate at the apex, spur 9-13 mm long lower petal 2-4 mm broad, not or slightly emarginate at the apex, spur 9-13 mm long lower petal 2-4 mm broad, not emarginate at the apex, spur 7-12 mm long lower petal 4-6 mm broad, slightly emarginate at the apex, spur 12-14 mm long Capsule narrowly lanceolate, 6-11 seeded, (8)10-18 × 2.5-5 mm narrowly lanceolate, 6-11 seeded, (12)14-24 × 3-5 mm lanceolate, 10-17 seeded, (12)13-18 × 2.5-4 mm lanceolate, 3-8 seeded, 18-28 × 2 mm Seed 1.5-1.8 mm in diam., elaiosome spirally twisted 1.5-1.9 mm in diam., elaiosome spirally twisted 1.2-1.4 mm in diam., elaiosome spirally twisted 1.6-2.2 mm in diam., elaiosome spirally twisted 346 ADANSONIA, sér. 3 • 2025 • 47 (21) Olcay B. et al. A C D E B fig. 3. — Herbarium specimen of Corydalis wendelboi subsp. fragilis subsp. nov., holotype ISTE118601: A, leaf; B, plant; C, raceme, flowers, fruits and bracts; D, bracts (more divided in maturity); E, seed. Scale bars: A, 2 cm; B, 3 cm; C, 1 cm; D, 3 mm; E, 1 mm. Photos by B. Olcay. 347 A new subspecies of Corydalis wendelboi Lidén from NW Türkiye (Papaveraceae) ADANSONIA, sér. 3 • 2025 • 47 (21) AB CD fig. 4. — Herbarium specimens of: A, Corydalis wendelboi subsp. fragilis subsp. nov., holotype ISTE118601; B, C. wendelboi subsp. wendelboi Lidén, specimen ISTE118585; C, C. wendelboi subsp. congesta Lidén & Zetterl., specimen ISTE118591; D, C. tauricola (Cullen & P.H.Davis) Lidén, specimen ISTE94344. Bracts are magnified and shown in a circle. Scale bars: full specimens, 3 cm; details; 5 mm. Photos by B. Olcay.