Beautiful, Magic, Lethal: a Social Perspective of Cinnabar Use and Mercury Exposure at the Valencina Copper Age Mega‑site (Spain)

Vol.:(0123456789) Journal of Archaeological Method and Theory https://doi.org/10.1007/s10816-023-09631-8 1 3 RESEARCH Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar Use andMercury Exposure attheValencina Copper Age Mega‑site (Spain) LeonardoGarcíaSanjuán1 · RaquelMonteroArtús1 · Steven D.Emslie2 · José AntonioLozanoRodríguez3 · MiriamLuciañez‑Triviño1 Accepted: 26 October 2023 © The Author(s) 2023, corrected publication 2023 Abstract Today, mercury is a matter of concern for health and environmental authorities across western countries, and legislation has been passed and programs have been implemented for its total elimination from human activity. But this was not always the case: mercury and its compounds have been highly appreciated and used since remote times all over the world with very diverse purposes ranging from decorative, medicinal, metallurgical and symbolic. In particular, cinnabar (HgS, mercury sulfide), a mineral of an intense red color, has been considered in many cultures as an exotic raw material, highly valued and associated with the elites and sacred practice. In this paper, we examine one such case, set almost 5000years ago, in Copper Age Iberia, by investigating mercury exposure through human bone. The study presented here includes a total of 170 samples from 70 different human individuals and 22 animals (plus one soil sample) from the Copper Age mega-site of Valencina, south-western Spain. It is the largest ever single-site study of exposure to mercury based on human bone in combination with cinnabar use. Abnormally high values are recorded in some individuals dating between 2900 and 2650 BC, especially in those buried in remarkable tombs belonging to the social elite of this period, but high levels of mercury are also recorded in the rest of the population. Three lines of interpretation are used to explain these results, including the manipulation of cinnabar (grinding it into powder, mixing it with other substances, using it for the decoration of objects, buildings and the human body), its direct consumption through ingestion or inhalation by a ‘special’ social group and the contribution of environmental factors. Based on the currently available evidence, which is carefully reviewed, Valencina represents the most intense and prolonged case of exposure to mercury recorded in human history, which makes it an important site to assess the long and complex history of use of this substance. Keywords Mercury· Cinnabar· Exposure· Copper Age· Iberia· Burial practices Extended author information available on the last page of the article L.García Sanjuán et al. 1 3 Introduction Mercury and cinnabar (HgS, mercury sulfide) have a long and complex history of use by human societies across the world. Both substances are amply documented in the Americas, East Asia and Western Europe in societies occurring in the last 10,000years. Their uses include a broad range of purposes, such as ceremonial and sumptuary display, pigment for painting, medicinal and magic drug, preservative and biocide and the extraction of precious metals such as gold and silver. For millennia, cinnabar, often mixed with other mineral substances such as iron oxides and organic binders (for example, plant oils and egg yolk), has been used as a pigment to paint rocky surfaces, wooden and stone buildings, artefacts, textiles or even the human body itself (Siddal, 2018; Gliozzo, 2021). Cinnabar pigment was used to paint the clay-modelled human skulls dating to the 9th millennium BC found in the Pre-Pottery Neolithic site of Kfar HaHoresh (Israel), where no cinnabar is locally available (Goren etal., 2001). In the Neolithic town of Çatalhöyük (Turkey), dating between the 8th and 6th millennia BC, human bones buried underneath the houses were painted with pigment made with red ochre and cinnabar, as were the walls of the houses themselves (Schotsmans etal., 2022). In Europe, the earliest documented use of cinnabar goes back to the Vinča culture of the middle Danube basin, named after the eponymous site in Serbia and dating to the 6th millennium BC, in which it was used to decorate objects and, probably, other purposes (Gajic-Kvascev etal., 2012; Mioč etal., 2004). In many cultures, cinnabar and mercury have also been used to provide health, prolong life or lead to immortality (Mahdihassan, 1987; Needham, 1976). Their use as medicinal or magic drugs is well attested. The use of mercury and cinnabar as a medicinal drug, as an elixir of life and to preserve cadavers, is well documented in China and India where these practices have continued until very recently (Liu etal., 2008; Needham, 1976). The alchemists of the court of Qin-Shi-Huang, first emperor of China (third century BC), sought the secrets of immortality in liquid mercury (Needham, 1976: 4). The trading of cinnabar along the ‘Silk Route’ is attested 2000years ago (Sevillano-López & González, 2011). Before the twentieth century, Western medicine made use of oral doses of mercury salts, alone or mixed with other ointments, as a sedative and to treat lice, fever, insomnia, brain traumas, ulcers in the mouth and tongue or syphilis (Biehler-Gomez etal., 2022; Fornaciari etal., 2011, 2022; Lombardo etal., 2022; Panova etal., 2018; Rasmussen etal., 2008). In the American continent, the use and exchange of cinnabar from the Huancavelica mines (Peru) are documented since the 3rd millennium BC (Prieto etal., 2016). In Mesoamerica, cinnabar was found in the graves of high-ranking individuals at Teotihuacan (México) (Sancho Mengod, 2013; Sugiyama & López Luján, 2006) and numerous Maya cities (Vázquez de Agredos, 2007; Batta etal., 2013; Ávila etal., 2014, Ochoa-Lugo etal., 2017; Cervini-Silva etal., 2018). Prominent among those is the tomb of the ‘Red Queen’ at Palenque (Cervini- Silva etal., 2013; González-Cruz, 2011). The Iberian Peninsula has an old and extensive record of use of cinnabar and mercury, which is reflected in a substantial literature—for recent reviews see 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… Zarzalejos Prieto, 2019; Zarzalejos Prieto etal., 2020a. Without question, the prominence of both minerals in Iberian Prehistory and History is explained by the presence in Almadén (located in Ciudad Real central Spain) of the largest mercury mine in the world. This site was included in the UNESCO World Heritage List in 2012. The oldest evidence of cinnabar use in Iberia known to this date is represented by the pigments recorded inside a clam shell (Glycymeris sp.) found at Cova de l´Or (Valencia), a cave located in the Spanish Levant and occupied in the Early Neolithic (second half of the 6th millennium BC) (Domingo etal., 2012). At Los Murciélagos cave (Zuheros, Córdoba), dating to the same period, cinnabar was used as part of the infill applied to the grooves of ceramic vessels and marble bracelets and also to coat the inner side of a pot (Martínez etal., 1999; Gavilán Ceballos etal., 2020). Cinnabar-dyed textiles of similar date have been recently published for a small cave located at Peñacalera, also in Córdoba (Gleba etal., 2021). At the flint mine of Casa Montero (Madrid), also dating to the second half of the 6th millennium BC, a knapped flint blade was found with traces of cinnabar, which were sourced to the Almadén mines (Hunt-Ortiz etal., 2011). Cinnabar has been found in numerous burial contexts dating to the 4th and 3rd millennia BC (Late Neolithic and Copper Age) across Iberia, either sprayed over the floors, bodies and grave goods of tombs or used as a pigment to paint the uprights and capstones of megaliths. Such is the case of a number of burial monuments (megaliths and hypogea) including Alberite (Cádiz) (Domínguez Bella & Morata Céspedes, 1995; Domínguez Bella, 2010), La Velilla (Palencia) (Zapatero Magdaleno, 1990; Martín Gil etal., 1995), Valle de las Higueras (Toledo; Bueno Ramírez etal., 2019), Getsemaní-Cerro del Ojo (Sevilla) (Bascón Mateos etal., 2016), Peñacalera (Córdoba) (Gleba etal., 2021), the Montelirio tholos and other burials located at the Valencina Copper Age mega (Sevilla) (Rogerio-Candelera etal., 2013; Bueno Ramírez etal., 2016; Emslie etal., 2022; etc.) as well as several others in southern Portugal (Inácio etal., 2013; Rocha etal., 2018) and central Spain (Garrido-Pena etal., 2019). Clear vestiges of the use of cinnabar have also been found in Bell-Beaker (Liesau etal., 2020) and Bronze Age burials (López Padilla etal., 2012) dating to the late 3rd and early 2nd millennium BC. In the Iron Age, the use of cinnabar pigment is well documented in high-end sculptures (Chapa Brunet etal., 2021) and pottery (Barrio Martín, 2020). Cinnabar and mercury were highly-valued commodities in Roman-time Hispania, when the Almadén mines were exploited extensively, as reported by Pliny in his ‘Universal History’ (Gil Bautista, 2015: 59–60). Cinnabar was used to produce the ‘Pompeian Red’ pigment applied to paint the walls of aristocratic houses and works of art across the Roman empire (Guiral Pelegrín & Íñiguez Berrozpe, 2020), while mercury was used to help extract gold at the mines of Las Médulas (León) (Zarzalejos Prieto, 2019; Zarzalejos Prieto etal., 2020b; Rodríguez García etal., 2021). Mercury was also well-known in Medieval Spain. The Almadén mines were mentioned by geographer Al Idrisi, in the twelfth century AD (Gil Bautista, 2015: 60), while in the Medina Azahara palatial complex located outside Córdoba, capital of the Spanish Caliphate between the tenth and eleventh centuries AD, there was a famous mercury ‘basin’ or ‘fountain’ that impressed L.García Sanjuán et al. 1 3 visitors (Molina, 2004). The exploitation of the Almadén mines peaked between the sixteenth and nineteenth centuries AD, when mercury was extensively used for gold mining in colonial Mexico and Peru, which involved a vast system for its transportation by land and sea set up by the Spanish Crown (González Tascón etal., 1996; VVAA, 1996; Gil Bautista, 2009, 2013, 2015). Because of its pervasiveness, and because of its long and complex history of exploitation, use, consumption and exposure, mercury is currently a topic of great scientific interest. This is largely explained by the implications of its presence in the environment and its negative impact for human health. Mercury occurs naturally in the environment. Half the emissions in the atmosphere come from primary sources (such as volcanic eruptions). In nature, it is found as metallic or elementary mercury (Hg°), organic mercury and inorganic mercury (Hg2+, Hg22+), while cinnabar is one of its natural inorganic forms (mercury sulfide, HgS). However, human exploitation, particularly in the last 2000years, but also quite earlier, has significantly increased its environmental occurrence and its impact. Until recently, mercury and cinnabar have been widely used for a multiplicity of industrial purposes and are therefore present in the daily lives of millions of people (Park & Zhen, 2012). Contemporary small-scale mining accounts for 37% of mercury emissions, while carbon combustion accounts for 24% (WHO,2017a; Bell etal., 2017; European Commission, 2017: 13). However, a great deal of the mercury currently present in the environment was released in pre-industrial times. A linear increase has been postulated starting c. 2000 BC (or earlier) until c. 1570 AD, followed by 300years of rapid increase caused by the exploitation of the Almadén mines for the smelting (‘amalgamate’) of silver and gold in the Spanish Empire and a constant level of emissions after c. 1850 AD (Amos etal., 2013:413). Pre-indus- trial emissions would account for as much as 39% of the mercury present in today’s environment, the remaining 61% having been caused by emissions post c. 1850 AD (Streets etal., 2011). Although the toxicological effects of mercury exposure in the human body are not known in detail, scientific research in the last century has led to a greatly improved knowledge and awareness of its dangers for human health. In turn, this has favoured the generalization of measures to reduce exposure (Bellanger etal., 2013;Risher, 2003). The current contemporary positioning of western science and public health policies against mercury is certainly well-grounded in solid science but also runs in stark contrast with its long history of use. Western medicine made extensive use of mercury as a medicinal drug until barely a century ago, as noted above. Indeed, in some regions of the world, cinnabar is still consumed today (ingested or inhaled), either for magic and religious reasons, as happens in the Caribbean (Wendroff, 2005), or as a medicinal drug, as in South Africa (Street etal., 2015) and Tibet (Gerke, 2021). Regardless of its past history and specific uses in the contemporary world, today, mercury is widely considered as a toxic metal, non-essential for the human body. It is currently listed by the World Health Organization as one of the ten most threatening chemical substances for public health worldwide (WHO, 2017b). The manifestation of its effects is closely related to the means of exposure. In turn, this is largely conditioned by the specific form of the mercury causing the exposure. Well-known 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… effects of mercury on human health include cardiovascular toxicity, reproductive and developmental toxicity, neurotoxicity, nephrotoxicity, immunotoxicity and carcinogenicity. Within the context of the long-standing and on-going interest in the scientific study of mercury and cinnabar use and exposure in human society, recent research based on the geochemical analysis of human bone has highlighted the social and cultural pervasiveness of both substances in Iberian Late Prehistory—see Emslie etal. (2015, 2016, 2019, 2022). As noted above, there is ample evidence of cinnabar use in Iberia during the 4th and 3rd millennia BC. One recent paper in particular has provided an overview on mercury exposure, based on the largest sampling of human bone ever undertaken, including 370 samples from 50 different burial structures (both individual and collective) of 23 different sites dating to the Late Neolithic (c. 4200–3200 BC), Copper Age (c. 3200–2300 BC) and Bronze Age (c. 2300–850 BC) (Emslie etal., 2022). Although this study showed a high variability of results (as was expected), unusually high levels of mercury were noted for some of the sampled human bones, particularly those dating to the Copper Age. While a major study centred on the site of La Lanzada (Pontevedra, Spain), a necropolis dating to the first to seventh centuries AD, which included 143 samples of 76 individuals (Álvarez- Fernández etal., 2020; López-Costas etal., 2020) analysed atmospheric mercury as the source for Hg in the bone, the approach followed by us looks at both environmental mercury and cinnabar as sources of exposure. In general, most previous studies of mercury in human bone recovered from archaeological contexts were based on a much smaller number of samples—see for example Cockburn etal., 1975: 1159; Yamada etal., 1995: 254–255; Torino etal., 2015; Bocca etal., 2018; Rasmussen etal., 2008, 2012, 2013a,b, 2015, 2019; Walser etal., 2019. This topic is further discussed below. The paper presented here is intended to further expand this line of research and, by focusing on a single site, namely Valencina de la Concepción-Castilleja de Guzmán (henceforth Valencina) located in Sevilla, south-west Spain, extend our knowledge of the use and exposure to cinnabar and mercury in Copper Age Iberia (Fig.1). For reasons that are briefly outlined below, Valencina currently stands as one of the most intensely researched and debated sites of 3rd millennium Europe. The study presented here includes a total of 170 samples from 70 different human individuals and 22 animals (plus one soil sample), which, together with abovementioned La Lanzada research, is the largest ever single-site study of exposure to mercury based on human bone, although centred on much older material. Some of the results included in this study (64 samples) were discussed in previous papers (Emslie etal., 2016, 2022), but the majority (106 samples, 62% of the total) are presented here for the first time. The contribution of this study lies not only in the high number of unpublished analytical results, which are combined with alreadypublished ones from the same site, but also in that the sampled individuals come from well-contextualised and well-dated burial structures occurring within a short time span of no more than 250years (c. 2900–2650 BC). This allows, for the first time in prehistoric studies, to assess cinnabar use and mercury exposure within the frame of a short number of human generations, leading to more precise conclusions in terms of the social and cultural background for which the substances were used. L.García Sanjuán et al. 1 3 Thus, the main aim of this paper is to present and assess all the results available for total mercury (THg) in human bone at Copper Age Valencina and to discuss possible hypotheses regarding the sources of exposure. We depart from the assumption that unexpectedly high levels of mercury in human and animal bone can stem from (i) environmental pollution, (ii) the pre-mortem use and manipulation of mercury and associated compounds (cinnabar), and (iii) post-mortem diagenetic processes. That at Valencina cinnabar was used in some high-ranking burials, coupled with evidence that it was thoroughly ground and sieved to produce a fine powder and then mixed with other red ochres to extend its availability (Rogerio-Candelera etal., 2013), very much suggests that it was valued as a high-end commodity. In turn, that some individuals reveal very high levels of mercury in their bones raises several questions regarding its use and its social and ideological significance. Contexts andSampling After the first discoveries in the 1860s and then gradual, if slow, advances throughout the twentieth century, research on the Valencina Copper Age mega-site has accelerated in the last two decades. The site is remarkable because of its large size (c. 450 hectares), monumentality, with massive ditches and some of Iberia’s largest Chalcolithic megaliths (including remarkable tholoi such as Montelirio, La Pastora and Matarrubilla), number and density of features (estimated in the tens of thousands, many of them funerary), as well as the amount and quality of the material Fig. 1 Main Iberian sites with THg measurement in human bone mentioned in the text (in red, sites with evidence of cinnabar). Design: Manuel Eleazar Costa Caramé and Raquel Montero Artús 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… culture found in them, which boasts some of the finest and most accomplished examples of 3rd millennium BC craftmanship across western Europe. Studies published in recent years include general overviews of the site (García Sanjuán etal., 2017), high-resolution approaches to some of its most important megalithic monuments and burials (Cáceres-Puro etal., 2014, 2019; Fernández Flores etal., 2016; García Sanjuán et al., 2019; Vargas Jiménez, 2021; etc.), extensive radiocarbon dating (García Sanjuán etal., 2018a), as well as detailed analyses of metallurgical (Murillo-Barroso etal., 2015; Nocete Calvo etal., 2008, 2014), lithic (Martínez- Sevilla etal., 2020; Nocete Calvo etal., 2005) and ivory technology (García Sanjuán etal., 2013; Nocete Calvo etal., 2013; Luciañez Triviño etal., 2022). On account of these advances, Valencina has featured prominently in recent reviews of Neolithic and Copper Age Europe—see for example Guilaine, 2018; Whittle, 2018: 11–12; Gaydarska & Chapman, 2022: 51–62. Previous studies have suggested the extent and importance of cinnabar use (Hunt Ortiz & Hurtado Pérez, 2010; Hunt-Ortiz et al., 2011; Rogerio-Candelera et al., 2013; Bueno Ramírez etal., 2016) and mercury exposure (Emslie etal., 2022) at Valencina, a body of evidence consistent with the role the site probably played as a central place of high social and religious significance across a large territory. This paper is intended to expand on that previous work. It is based, as noted above, on a total of 170 samples obtained over the last 5years. Figure2 shows the location of the various Valencina and PP4-Montelirio sectors and features sampled in this study. In total, 53 samples from 23 different individuals come from the Montelirio Copper Age tholos (22 individuals of Chalcolithic age and one individual, buried outside the tholos, of Early Neolithic chronology) (Table1 and Supplementary Data SD 1). Another 94 samples come from the adjacent PP4-Montelirio sector, including 89 samples from 42 Copper Age individuals found across 22 different burial structures (Table2 and Supplementary Data SD 2), as well as five samples from as many Roman-time individuals found in as many graves (Hg results published in Emslie etal., 2022). Regarding to the sampling of animals, 22 samples were taken, six from PP4-Montelirio sector and 16 from three features located in others sectors of the Valencina mega-site (Pabellón Cubierto, Calle Mariana de Pineda and Calle Huelva) (Table3). Finally, also one soil sample was taken. This represents the largest singlesite sampling program of mercury in human bone ever undertaken for a prehistoric site and is aimed at exploring the social use and cultural significance of cinnabar at the Valencina mega-site and in Copper Age Iberia at large. Among the numerous structures sampled for this study (see full list in Table1 and Table2), two stand out on account of their architectural sophistication as well as the wealth of the grave goods laid in them: The Montelirio tholos (Fernández Flores etal., 2016) and Structure 10.042–10.049 (García Sanjuán etal., 2019), both located on the south-eastern quadrant of the site (Fig.2). Structure 10.042–10.049 and the Montelirio tholos stand next to each other and are part of the same temporal and cultural dynamics of burial use of the south-eastern sector of the site between c. 2900 and 2650 BC. However, they were excavated by different teams at different times, and for that reason, their study has progressed in part separately. According to the available radiocarbon chronology, Structure 10.042–10.049 (a semi-destroyed double tomb) was likely built between 2900 and 2800 BC (García L.García Sanjuán et al. 1 3 Fig. 2 A Location of Valencina sectors with THg measurement. B Location of the Montelirio tholos and 10.042–10.049 structure within the PP4-Montelirio sector. Design: Manuel Eleazar Costa Caramé and Raquel Montero Artús 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… Table 1 THg (µg/g) content (max) in bones for 23 individuals buried in the Montelirio tholos (the SC contained a MNI = 2, but three samples were analysed) Structure Individual THg (µg/g) sd (µg/g) Bone Age Sex Chronology Pathology MSSMaGarment Other Source Large Chamber UE 115 483.97 102.94 F Adult ¿? - NO -NO Cut by UI64 This paper UE 112 273.52 38.32 H 30–35 F - NO HRG NO Cut by UI64, PA diet Emslie etal., 2016 UE 346 255.88 43.17 H 25–29 F - Osteoarthritis HRG NO Cut by UI64 Emslie etal., 2016 UE 104 207.15 31.72 F 25–34 F - Osteoarthritis - NO - This paper UE 103 156.01 5.73 F 25–29 F 4220 ± 17 Osteoarthritis HRG, FAT Skirt / Belt - This paper UE 113 132.21 38.29 F 30–35 F 4116 ± 24 Osteoarthritis - NO Lower level Emslie etal., 2016 UE 107 123.02 34.84 H 35–39 F - Osteoarthritis - NO PA diet Emslie etal., 2016 UE 108 116.14 12.31 H 30–45 F - Osteoarthritis - NO - This paper UE 114 110.30 10.58 F 18–23 F - NO - Skirt / Belt Cut by UI64 This paper UE 101 92.26 2.19 H 25–29 F - Periostitis HRG, FAT Skirt / Belt Polydactyly This paper UE 109 84.14 15.26 H 18–25 F - Periostitis IOF NO - Emslie etal., 2016 UE 105 55.23 2.09 H 18–25 F 4164 ± 36 NO -NO - This paper UE 102 52.83 6.97 T 25–34 F 4195 ± 22 NO HRG Full costume To the east of stela This paper UE 111 50.21 6.48 H 20–29 F - NO - Beads under shoulder ¿rug? By stela Emslie etal., 2016 UE 396 30.55 3.84 FIB Adult ¿? - NO -NO Cut by UI340 This paper UE 110 10.67 0.93 F 22–24 F 4129 ± 33 NO -NO Cut by UI340 Emslie etal., 2016 UE 343 8.77 0.36 H 24–32 F 4168 ± 30 NO - Full costume Cut by UI340 This paper L.García Sanjuán et al. 1 3 Fig. 3 Examples of the use of cinnabar in Valencina. A, C, D Montelirio Large Chamber: cinnabar powder on human bones and beads, painted baetyls. B Montelirio corridor: decorated slabs. E Cinnabar powder sprayed on Structure 10.049. Photos: A: Álvaro Fernández Flores and C: Antonio Acedo García (from Fernández Flores & García Sanjuán, 2016); B: Antonio Acedo García (from García Sanjuán etal., 2016); D: adapted from Rodrigo de Balbín Behrmann (from Bueno Ramírez etal., 2016); E: photo: José Peinado Cucarella; drawing: Miriam Lucianez Triviño (from García Sanjuán etal., 2019) 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… samples were analysed twice of thrice to obtain an average THg in µg/g (ppm). Each of the measurements was preceded and followed by two samples of two standard reference materials (DORM-4 and DOLT-5 or DORM-4 and TORT3). The results are presented as means ± standard deviation of the values obtained. For individuals with more than one sampled bone, the highest THg value is used. The results were analysed quantitatively and comparatively to assess potentially significant differences in the occurrence of mercury among different individuals in different contexts and regarding a series of variables. To achieve this, a Shapiro–Wilk normality test, Mann–Whitney’s U and χ2 significance tests were carried out at various times, as described below. To better understand the data, intervals were established to distinguish between ‘very high’ (> 100µg/g), ‘high’ (10–100µg/g), ´moderate´ (1–10µg/g) and `low’ (below 1µg/g) THg values. Results Montelirio Tholos A human contingent of 26 people was found in Montelirio, including 20 individuals in the Large Chamber (LC), two in the Small Chamber (SC), three in the Main Corridor (MC), which leads to the LC from the exterior, and one outside the megalith (Pecero Espín, 2016: 410). The individual buried outside the megalithic structure yielded a much older radiocarbon age (OxA-X-2535–32, 4727–4547cal 2σ BC) and therefore represents an earlier burial episode at the site (García Sanjuán etal., 2018a, b: 208; Table1). In total, 50 values of total 0.10 1.00 10.00 100.00 1000.00 UE 115 UE 112 UE 346 UE 103 UE 107 UE 108 UE 114 UE 101 UE 109 UE 105 UE 111 UE 396 UE 102 UE 113 UE 110 UE 104 UE 343 UE 80 UE 88 UE 73 UE 229 UE 251 UE 232 UE 273 Fig. 4 THg concentration (± sd) in human bone (µg/g) for 23 individuals buried in Montelirio Tholos (17 in LC, 2 in SC (3 SUs), 3 in corridor, 1 outside). The SC contained a MNI = 2, but three samples were analysed) L.García Sanjuán et al. 1 3 mercury THg (µg/g) are available for the Montelirio tholos, covering 23 of the 26 individuals buried in it (the ones excluded are UE116, UE356 and UE398, all from the LC; Fernández Flores etal., 2016; Fig.4). While 26 of those values have already been published (Emslie etal., 2016), the remaining 24, corresponding to 12 individuals (10 in the LC and 2 in the SC), are presented here for the first time. In general, mercury values recorded in the Montelirio human bones are very high. While they all rise above 1µg/g, 83% (excluding the individual buried outside the megalithic structure) are above 10µg/g, with average total mercury concentrations of 81.92µg/g, 95.20µg/g and 18.79µg/g in humeri, femora and tibiae, respectively. Recorded values are particularly high for the 17 individuals from the LC, with values ranging from a maximum of 483.97 ± 102.94µg/g (individual UE115, femur) to a minimum of 8.77 ± 0.36µg/g (individual UE343, humerus). In the SC, both individuals present levels higher than 10 µg/g (19.61 ± 1.50 b–38.10 ± 2.39µg/g). The lowest values are recorded in the three individuals buried in the MC (2.00 ± 0.44µg/g–4.89 ± 0.32µg/g). The human remains analysed from the LC belong to adult women except in two cases (individuals UE115 and UE396), for which sex could not be established through conventional anthropological examination (Pecero Espín, 2016: 427). The bodies were in anatomical connection, occupying the whole space of the LC, except the area leading to the SC. It is important to note that these bodies were carefully arranged around a stela or altar made in unfired clay that was found at the centre of the LC, with some ceramic plates and other objects surrounding it (Fig.5). Two subgroups of individuals can be discerned according to total THg levels. The first subgroup includes nine individuals (eight women, one sexually undetermined) with maximum values between 110.30 ± 10.58 and 483.97 ± 102.94 µg/g (four each in femora and humeri). That means that up to 53% of the individuals sampled in the LC have total mercury values above 100 µg/g (Fig. 5a). Generally, the individuals of this subgroup were between 25 and 45years of age at the time of death, with only one exception (individual UE114, who was 18–23years old). They were mostly laid in the south-eastern sector of the LC and according to their relative vertical position (showing the sequence of deposition) were the first to be deposited in the tomb with the only exception of individual UE104, who was one the latest to be buried (García Sanjuán etal., 2016:519). This subgroup includes all the women buried in the tholos (six in total) who all exhibit a degenerative condition (arthrosis in the dorsal vertebrae, shoulders and lower limbs) caused by ageing or functional wear (Pecero Espín, 2016: 434–435). In addition, three of these women showed an anomalous development of the first gluteal tuberosity of the femur in connection with higher muscle work and blood flow, in turn connected with sustained physical activity involving the legs (Pecero Espín, 2016: 432). Individuals UE112 and UE107 Fig. 5 Graphic recreations of the distribution of individuals in the LC of Montelirio according to THg levels. Above, THg > 100ppm; Below, THg: 1–100ppm. Drawings: Miriam Luciañez Triviño (adapted from Pecero Espín, 2016) ▸ 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… L.García Sanjuán et al. 1 3 showed higher levels of animal protein consumption, which could be explained by their enjoying a higher social status (Fontanals Coll etal., 2016: 446), possibly also in connection with their older age. The second subgroup includes seven adult females and one adult of undetermined sex with THg levels below 100µg/g (Fig.5b) and maximum values between 8.77 ± 0.36 and 92.26 ± 2.19µg/g (five of them measured in humeri). In general, these individuals were younger, between 18 and 34years of age at the time of death. They were buried partly above the previous subgroup, in two different areas of the chamber: individuals UE110, UE396, UE111, UE109 and UE343 were laying on the western side, closer to the clay stela/altar, while the other three (UE102, UE101, UE105) were laid closer to the entrance of the LC, next to a set of large ceramic plates and clearly above the first subgroup. In general, the individuals of this subgroup with lower THg levels do not display any pathologies, while those two with comparatively higher THg levels (UE109 and UE101) present periostitis of the femora and tibiae, respectively. In addition, one woman (UE101) had squatting facets in both tibiae and polydactyly (six toes) in both feet (Pecero Espín, 2016: 436). The latter condition was undoubtedly a very visible and remarkable physical trait that probably made this woman quite special in her time. Women UE101 and UE102, who were laid closely together, also showed hyper-development of the first gluteal tuberosity of the femur. In the SC, which was badly altered by a re-use in Roman times, only a small number of poorly preserved human remains was found. They revealed two adult individuals, one female and one sexually indeterminate (Pecero Espín, 2016: 423), dating to the same period as those in the LC (Bayliss etal., 2016). Mercury was measured on anatomically-unspecific bones from stratigraphic units UE80 and UE88, revealing THg concentrations ranging between 19.61 ± 1.50µg/g and 38.10 ± 2.39µg/g, which are similar to those of the second subgroup of the LC described above. The central section of the MC yielded three inhumations, two of which were secondary (individuals UE229, a male adult, and UE251, a probably female adult) and one primary (individual U232, a sexually undetermined adult) (Pecero Espín, 2016: 423–425), as well as numerous offerings, including plants, animals and artefacts. Mercury levels measured in the bones of these three individuals were significantly lower than those from both chambers, ranging between 2.00 ± 0.44 and 4.89 ± 0.32µg/g. Individual UE232, showing the lowest THg concentrations, was buried with a small ceramic vessel placed under his hand. Individual UE273, who was between 35 and 55years old at the time of death, was found outside the MC, on the left-hand side of the MC (as one enters). Both his stratigraphic position and one radiocarbon date show this individual predates the construction of the tholos by several centuries. The bones of this subject had mercury levels at 4.32 ± 0.82µg/g. PP4‑Montelirio Sector As described above, the PP4-Montelirio sector, comprising 134 features that surround ‘TheIvory Lady’ grave, lies adjacent to the north to the Montelirio tholos (Mora Molina et al., 2013). In total, 94 bone samples belonging to 47 different 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… human individuals (89 Chalcolithic and five Roman ones) from this sector have been analysed (Table2), Emslie etal., 2022). Only for 18 of them was it possible to measure THg concentrations for all three target bones (humerus, femur and tibia). The sampling for this sector encompasses 42 Copper Age individuals (89 results) buried in 22 different burials that break down as follows: three megalithic structures, 12 negative features (pits or basins) and seven negative features with stone elements (Fig.6). Seven of those features are individual burials, while 15 were collective. In this paper, 59 measurements of THg concentrations for 31 of those Copper Age individuals (found in 20 different structures) are presented for the first time). The sampling of the PP4-Montelirio also includes five Roman-time individuals from as many graves found on the northern limit of the sector. The results for the Roman burials were presented in a previous paper (Emslie etal., 2022) but are also discussed here as a control group for the 3rd-millennium population. Cinnabar powder was only observed in two of the 27 burials (22 dating to the Copper Age, five to Roman time), namely structures 10.042–10.049 and 10.074, which, as is discussed next, are the ones with the bones showing the highest mercury values. The results for the Copper Age population of the PP4-Montelirio sector presents a high variability of THg, ranging from 0.19 to 480.50µg/g. Up to 45% of the individuals of this sector having mercury levels above 1µg/g, in particular those buried in megalithic structures. Half of the 22 burials analysed include at least one individual with mercury levels above 1µg/g, and in eight of them (36%), measurements are above 10µg/g (10.18 ± 0.66–480.50 ± 19.58µg/g). The second highest recorded mercury levels correspond to ‘TheIvory Lady’ (THg value of 185.54 ± 10.65µg/g), while the two individuals found in Structure 10.042 Fig. 6 Percentage of individuals according to THg levels for each type of funerary structure in PP4-Mon- telirio sector L.García Sanjuán et al. 1 3 (indeterminate sex) returned values of 19.70 ± 1.62 and 28.10 ± 6.23µg/g. Substantial amounts of cinnabar powder were sprayed in both chambers. Interestingly, however, the highest mercury value was not recorded in Structure 10.042–10.049, but in an adult person of indeterminate sex found in Structure 10.074, a single inhumation in a simple pit of small size (1.60 × 1.40m), irregular plan and no stone elements (Robles Carrasco etal., 2017:106). The extremely high value recorded for the person buried in Structure 10.074 (480.50 ± 19.58µg/g in the radius) is on par with the highest recorded at the Montelirio tholos (individual UE115, 483.97 ± 102.94µg/g). Mercury measurements for a rib of this individual, however, yielded much smaller values (16.55 ± 2.29µg/g). Cinnabar powder as well as a scallop shell (Pecten maximus) were identified inside Structure 10.074 (Liesau etal., 2014: 96). In principle, although the configuration of the grave (simple pit with no stone elements) would not lend the person buried inside Structure 10.074 a very high social standing, the presence of cinnabar and a scallop shell suggest some kind of social prominence. Scallop shells must have had a high symbolic value within the Copper Age world view1 as they were used as grave goods in 16 other graves in the PP4-Montelirio sector (Liesau etal., 2014: 82–83) and to manufacture the perforated beads of the garments worn by some of the women found in the LC of Montelirio (Díaz-Guar- damino etal., 2016). Mercury values are available for the bones of six of the persons who received scallop shells as grave goods. Three of them recorded high (> 10µg/g) or very high values (> 100 µg/g, Structure 10.074), while the others showed much lower ones: Structure 10.034, a single inhumation, returned a THg value of 1.33 ± 0.14 µg/gµg/g, and Structures 10.012 and 10.040 yielded values < 1 µg/g (although data are not available for all the individuals buried in these two graves). It should be noted that in the analysed PP4-Montelirio burials, whether individual or collective, where mercury levels > 10µg/g are recorded, no evidence of cinnabar powder has been found. Structure 10.035, a simple pit with no grave goods and no cinnabar powder, contained two individuals. One of them, an adult of undetermined sex, exhibited osteitis in an ulna (Robles Carrasco etal., 2017:107) and returned a THg value of 52.29 ± 4.68µg/g. The other individual is an infant between 7 and 10years of age, the only surviving elements being the teeth (Cintas-Peña etal., 2018: 98). Structure 10.073 is a negative feature with some stone elements that yielded a minimum number of three individuals as well as grave goods consisting of three ceramic vessels, a marine shell (Cymbula nigra) and a copper punch with a handle made in worked osseous material (probably animal bone). Two of these individuals, between 26 and 40years of age and likely female, returned high THg concentrations (28.08 ± 2.50µg/g and 19.70 ± 1.62µg/g). One of them presented stress marks in the form of enthesopathies in the left radius bone (Robles Carrasco etal., 2017:107). Structure 10.044 is also a negative feature with some stones in which a minimum number of three individuals was recorded accompanied by very simple set of grave goods consisting of some fragmented ceramic vessels, one whole vessel and a small unworked stone. All three individuals were analysed for mercury, yielding values 1 The earliest known symbolic use of these shells in Iberia goes back to c. 35,000years BP and is attributed to late Neanderthals (Zilhão etal., 2010). 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… of 17.13 ± 2.67µg/g, 2.48 ± 0.05µg/g and 0.38 ± 0.01µg/g. Individual #2 presented stress marks in the form of enthesopathies in the femur and collarbone (Robles Carrasco etal., 2017:110). Structure 10.028 is a simple pit 1.7-m across, containing a MNI of 9 individuals distributed in two levels: the lower level contained five individuals, while the upper level yielded four individuals arranged radially. Eight of them were analysed for mercury. Five returned values above 1µg/g (between 16.31 ± 0.96 and 1.14 ± 0.07µg/g) while the other three yielded values between 0.86 ± 0.01 and 0.69 ± 0.02µg/g. Structure 10.075–10.078 is a negative feature with stone elements. It presents a chamber circular in plan, an access corridor and several slate slabs at the entrance. Two depositional levels were recorded; in the lower level (10.075), a single individual was accompanied by a whole ceramic vessel and fragments of a second, incomplete one next to the hands; in the upper level (10.078), three primary inhumations were recorded alongside three ceramic vessels, a needle or punch of worked bone and a flint blade. Mercury concentrations were measured for the individual found in the lower level and for two from the upper level. All THg values were < 1µg/g except for one of the individuals in the upper level which yielded relatively high concentrations (THg 10.18 ± 0.66µg/g) compared to all other individuals from this feature. PP4‑Montelirio Sector: Roman‑Time Structures Alongside the Copper Age features, five Late Roman burials were found at the PP4- Montelirio sector, including some simple pits and small chambers made with brick walls (García Sanjuán etal., in preparation). In none of them was cinnabar powder recorded. Mercury contents were analysed for the humeri of five individuals (structures 10.020, 10.045, 10.057, 10.009 and 10.058), showing average values of 0.32 ± 0.52µg/g, in all cases below 1µg/g (0.04–0.20µg/g), except for the individual found in Structure 10.020 (THg 1.24µg/g; Emslie etal., 2022). As will be discussed below, these values are in line with two other studies of mercury in human bone of Roman chronology in Iberia. Faunal Remains andSoil Samples As well as human bone, as part of this study, mercury concentrations were measured in 22 samples of animal bones from the PP4-Montelirio (6 samples) and other Valencina sectors (16 samples; Table3). From Structure 10.042, six poorly preserved animal bones from stratigraphic units UE640 and UE648 were analysed. The first group of samples (UE640) yielded very high values, between 32.57 ± 8.44 and 120.68 ± 8.06 µg/g, the latter corresponding to a deer antler that showed signs of thermal stress and had been probably used as a tool (Liesau etal., 2014: 90). In turn, all THg values for UE648 were below 1µg/g (0.46 ± 0.18–0.79 ± 0.04µg/g). The Pabellón Cubierto animal bone samples yielded high values for sheep/goat, with THg concentrations of 14.44µg/g and 11.17µg/g. Animal bone samples from Mariana Pineda Street and Huelva Street L.García Sanjuán et al. 1 3 Table 3 Comparison of mercury levels (µg/g) in animal bones from different prehistoric sites and recent times (*included prehistoric sites near Almadén mines, Ciudad Real) Period Site nMedia sd Min Max Source Chalcolithic Sector PP4- Valencina (10.042, UE640) 3 69.42 45.79 32.57 120.68 This paper Sector PP4- Valencina (10.042, UE648) 3 0.649 0.171 0.457 0.787 This paper Valencina (Pabellón Cubierto) 13 4.727 4.289 1.540 14.44 This paper Valencina (others) 3 0.270 0.144 0.110 0.390 This paper Perdigões 5 0.016 0.014 0.008 0.041 Emslie etal., 2015 Los Cercados, Las Quintanas, Melgar de Abajo, El Castillo (Valladolid) 10 0.018 0.016 0.007 0.050 Logemann etal., 1994 Bronze Age El Balconcillo (Soria), Cueva de Pedro Fernández and Arenero Soto (Madrid), Cerro de la Encantada and Motilla de Sta. María del Retamar (Ciudad Real), Cerro de la Campana (Murcia) 34 0.161 0.261 0.008 1.373* Logemann etal., 1994 Iron Age El Castillejo (Soria) 17 < 0.006 - - - Logemann etal., 1994 Recent time Almadén (Ciudad Real), Teruel 15 0.079 4.472 0.005 0.887 Logemann etal., 1994 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… yielded lower values (0.11–0.39µg/g). In none of the features from which these 16 samples were retrieved was cinnabar powder observed. A soil sample from inside the shaft of an animal long bone (the radius of an ovicaprid with THg: 2.73µg/g) located in the Pabellón Cubierto sector was also analysed, yielding a THg concentration of 14.50µg/g. Discussion Exceptionally High levels? In the context of evidence available for Iberian Late Prehistory, with 23 sampled sites (Emslie etal., 2022), the first observation that stands out from the data described above is the exceptionally and pervasively high values of mercury in Copper Age human and animal bone (and even in the only soil sample available) at Valencina— a full synthesis of the THg values is presented in Supplementary data SD 1 and 2. Copper Age Valencina has yielded the highest values of mercury in human bone recorded in Iberia to date, with concentrations above 400µg/g at Montelirio and Structure 10.074 and several occurrences of values above 100µg/g (Table4, Fig.7). Notably, about 65% of all Valencina individuals produced concentrations of THg higher than 1µg/g while the individual with the lowest level had only 0.19µg/g. It is also important to underline that this appears to be strictly a Copper Age phenomenon, as the values obtained for the PP4-Montelirio individuals of Roman age are much lower, with an average concentration of 0.32µg/g (Tables4 and 5). But how high are the values obtained for Copper Age Valencina relative to other sites? A main source of comparison is the data currently available for Neolithic and Copper Age Iberia, between the 5th and 2nd millennia BC (Table6; Emslie etal., 2022). This evidence shows a non-homogeneous distribution of mercury values over Table 4 Total mercury concentrations (µg/g) at Valencina nAverage sd Min Max Montelirio tholos Large chamber 17 131.93 119.69 8.77 483.97 Small chamber 2 28.82 9.25 19.61 38.1 Corridor 3 3.57 1.46 2.00 4.89 Exterior 1 4.32 0.82 4.32 4.32 PP4—Montelirio sector Chalcolithic burials 42 21.17 78.42 0.19 480.5 Roman burials 5 0.32 0.52 0.04 1.24 Animals 6 35.03 47.53 0.46 120.68 Other sectors Animals 16 3.89 4.24 0.11 14.4 Soil 1 14.5 - 14.5 14.5 L.García Sanjuán et al. 1 3 mercury values (> 10µg/g) are recorded in humeri, followed by femora and tibiae, which is in line with observations made in previous papers (Emslie etal., 2015: 2; 2019). This higher accumulation of mercury in one type of bone (humerus) would directly disprove the diagenetic contamination hypothesis, as the post-mortem incorporation of Hg from the cinnabar sprayed in the tombs would not ‘select’ which bones to accumulate in. This has been further discussed in a previous paper by Álvarez-Fernández etal. (2022). As noted above, only for 28 out of the 65 Valencina individuals was it possible to measure mercury in all three target bones (humerus (H), femur (F) and tibia (T)), totalling 84 THg values. Considering that measurements in different bones are not always available for the same individuals, the results presented in Tables1 and 2 show the maximum levels recorded for each individual, regardless of the sampled bone. Figure8 shows the total mercury content according to bone type for those 28 individuals. While femora and tibiae appear to show roughly similar values, the figures for humeri are higher, which was noted by Emslie etal. (2019). To determine whether these apparent differences are statistically significant, various tests were applied. First, a Shapiro–Wilk normality test (< 50 observations) with a 95% significance level, yielded a value of p > 0.05 which, entails the rejection of the null hypothesis according to which mercury would be normally distributed in human bones. In addition, non-parametric Mann–Whitney’s U significance tests (p < 0.05) were calculated for various combinations of bones in that subsample, including H-F, H-T and F-T. Values of p > 0.05 (p = 1 (H-F), p = 0.403 (H-T) and p = 0.623 (F-T)) were obtained, and therefore, H0 stating that no significant difference exists in mercury concentrations among different bones must be accepted. Additional χ2 tests were used to look at possible statistically significant differences in the distribution of THg in the three target bones, with an α value of p < 0.05. While different tests were carried out at different concentration intervals (< 1µg/g, 1–10µg/g, Fig. 8 Total mercury content (µg/g) at Valencina site according to bone type 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… 10–100 µg/g, > 100µg/g; Table 5), no differences were found. Therefore, for this particular group of samples, no statistically significant differences were found in the Mann–Whitney’s U and χ2 tests, which, in principle, would run counter to previous assumptions that mercury values in humeri were higher than in femora and tibiae (Emslie etal., 2016, 2019: 7–8). No effect of type of bone has been noted in previous studies (Álvarez-Fernández etal., 2020: 5). The distribution of mercury in bone tissue has been examined in laboratory studies that suggested higher accumulations in the skull than in humeri and femora (Halffman, 2009). The reason for this may be physiological, such as variations in blood flow, but also ‘external’, such as differences in the pre-treatment of bone samples. Among the latter, it is worth noting the inclusion of trabecular tissue in skull samples but not in those on long bones, or the elimination process of the ‘fatty part’ of femora, where mercury could potentially accumulate (Halffman, 2009: 154). The presence of higher mercury values in trabecular versus cortical tissue has also been noted in a study of long bones of Medieval individuals (Rasmussen etal., 2013a). In our study, samples were always taken from cortical bone, and therefore’external’ variations can be ruled out to explain potential differences in mercury values across different bones. Studies investigating the concentration of other metals showed variable concentrations in different bones, but the pattern is also related to the age factor. Thus, in a sample of 134 individuals who had experienced a ‘normal’ exposure to lead, adults showed higher concentration in their tibiae than in the crania, whereas younger subjects (between 21 and 35years of age) showed more uniform distributions of the metal across the skeleton (Wittmers etal., 1988). A suggested explanation for this phenomenon is that, in the long term, bones with a higher proportion of cortical tissue, such as tibiae, display higher lead concentrations than bones with a higher proportion of trabecular bone, such as the cranium. This study has important implications for the study of mercury exposure among past societies as the only available samples are bones (normally, unless mummies exist) and underlines the need to examine intra-skeletal variability, while at the same time controlling other biological factors such as age. In the case of the Valencina population, the subgroup for which measurements in three bone types are available (n = 28) is too small. In addition, there is no evidence pertaining to factors such as time, frequency and duration of exposure or, in some cases, age and/or sex of the individual (but see below). Therefore, the analysis of the intra-skeletal variability of mercury at Valencina will have to wait until the sampled population is expanded, and more data are available. But it is important to note that the negative results of the significance test described above does not allow the use of differential pre-mortem rates of accumulation as an argument against potential diagenetic factors. Mercury behaviour in graves is complex, and many different aspects must be taken into account to analyse the interaction between mercury from the bones and the surrounding soil (Álvarez-Fernández etal., 2021, 2022). Variability bySex andAge At Copper Age, Valencina there appears to exist a correlation between high mercury levels and the sex of the individuals, at least in the two main megalithic burials L.García Sanjuán et al. 1 3 included in this study, namely Structure 10.042–10.049 and the Montelirio tholos, in both of which women display very high THg levels. However, the sample available appears to have a severe demographic bias. While in Structure 10.042–10.049, only one individual, ‘The Ivory Lady’, was sexed; in Montelirio, all the individuals for whom sex could be established were females, with only one exception. Clearly, the Montelirio tholos did not house a ‘natural’ representation of the society that built it, as no elderly individuals, children and virtually no men were buried in it. However, it is interesting to note that the only confirmed male subject, a secondary inhumation found in the Main Corridor, showed the lowest mercury values for the whole of the monument (3.82µg/g). A similar concentration (4.32µg/g) is recorded for the individual buried outside the megalithic structure, also a male, who, nevertheless dates to the Early Neolithic and therefore cannot be compared with the contingent buried inside the tholos. For the burials of the PP4-Montelirio sector, sex could only be established for a little over 70% of the individuals involved in this study, including 12 indeterminate, 13 females or possible females and five males (Robles Carrasco etal., 2017). Only two male individuals yielded THg values around 20µg/g (17.13 and 19.70µg/g), while the other three showed values ranging between 0.40 and 0.98µg/g. Among the most exposed individuals (> 10µg/g), females have much higher THg values than males (40% vs 20%). As for age, it is worth noting that at Montelirio older individuals tend to have higher THg levels. Individuals with values above 100µg/g showed ages between 25 and 45years (except UE114, who was 18–23 at the time of death), while individuals between 10 and 100µg/g are younger, ranging between 18 and 34years of age. There could be two reasons for this: (i) older people had greater lifetime exposure and bioaccumulation; (ii) on account of their seniority, older people were assigned tasks more directly connected with the handling, use and/or consumption of cinnabar more often than younger people. Intra‑site Variability At Valencina, cinnabar has been reported in a small number of structures, including Structures 10.042–10.049 and 10.074 in the PP4-Montelirio sector and the Montelirio tholos. Red pigments, often vaguely referred to as ‘ochre’, are mentioned in other excavation reports, but there is no analytical evidence indicating that they included cinnabar.4 And yet, THg values are generally high in human bone for the whole of Valencina. This contrasting evidence (small number of features with cinnabar vs. pervasive presence of mercury in human bone) raises a number of questions; what was the social dimension of cinnabar? Was there 4 A case in point of particular interest is the tholos-type monument recorded in the early 1990s barely 50km to the south of Montelirio, often referred to as the Depósito de Agua (‘Water Cistern’) tholos. Although this monument was wantonly destroyed by order of the Castilleja de Guzmán municipality, a crime never investigated by the heritage authorities, the available photos and descriptions suggest a presence of cinnabar not unlike that of Montelirio (Santana Falcón, 1991). 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… significant variability of its use across the site? Did the intensity of its usage change through time? A Mann–Whitney U significance test (p < 0.01) revealed statistically significant differences in the amount of THg in bone between the individuals buried in the Montelirio tholos and those buried in the neighbouring PP4-Montelirio sector (p: 5.4 × 10−15), and also between those buried in megalithic vs non-megalithic burials (p: 1.6 × 10−17). Departing from the assumption that cinnabar was a high-end commodity, probably only accessible to individuals of certain social standing, these differences support the (otherwise apparent) comparatively higher social status of the group buried in Montelirio and, generally, of those buried in megalithic graves. In general, when analysing the intra-site variability of cinnabar use and mercury exposure at Valencina, the following can be noted: (i) the values recorded in the individuals buried in Montelirio are the highest for the entire site, while at the same time, in no other burial, a group so large had such consistently high THg values; (ii) the Montelirio contingent was endowed with finely crafted high-end artefacts, in many cases manufactured from foreign raw materials which, within the whole of Valencina, are only matched by those found next to and above ‘The Ivory Lady’; (iii) cinnabar has a pervasive presence at Montelirio (in the form of coated and painted slabs as well as sprayed-on bodies and grave-goods), but at the PP4-Mon- telirio sector, it was only recorded in two features (structures 10.042–10.049 and 10.074); (iv) while Montelirio is a collective burial, the two individuals with the highest THg values (> 100µg/g) at the PP4-Montelirio sector were buried in single graves; (v) while all individuals buried in Montelirio showed Hg exposure and had therefore experienced some degree of interaction with cinnabar in their lives; at the PP4-Montelirio sector, only one part of the population (45% of individuals) shows Hg exposure (THg > 1µg/g)]; (vi) the variability of mercury levels within the Montelirio contingent is lower (one order of magnitude) than that of the PP4-Montelirio (two orders of magnitude). It is also worth noting that the individual most exposed to mercury within the PP4-Montelirio sector (on par with the highest recorded at the Montelirio tholos) was in Structure 10.074, a single inhumation in a simple pit of small size. Persons buried in other graves where scallop shells were also found (namely structures 10.040, 10.012 and 10.080) appear to have very low THg levels. Therefore, neither in terms of the architecture of this grave nor in terms of the grave goods (a scallop shell and some sprayed cinnabar), this individual appears to have a social standing akin to that of ‘The Ivory Lady’ or those buried in the two chambers of Montelirio. In this regard, it is also worth remarking that not all individuals exposed to mercury in Valencina were buried in graves where cinnabar was used as part of the funerary ritual. In fact, only in the two features mentioned above (structures 10.042–10.049 and 10.074) was cinnabar observed. The same is found in other Late Neolithic and Copper Age burials across the Iberian south-west. At sites such as Sobreira da Cima, Santa Rita and Perdigões (Tombs I and II), where high Hg values in human bone were detected, cinnabar was found. At the sites Cabeco Arruda and Paimogo I, cinnabar was found in the burials, but the analysis for mercury revealed no exposure. Inversely, at Outeiro 2 and Monte Malheiro 2, cinnabar was not found in the tombs, but some people showed very high mercury values, above > 100µg/g. L.García Sanjuán et al. 1 3 These are important observations for two reasons, first, because they support the hypothesis of substantial pre-mortem exposure to mercury, as opposed to a postmortem, diagenetic hypothesis—see discussion in Emslie etal. (2019). Bones buried without cinnabar in association cannot have experienced diagenetic absorption of mercury. Second, because they suggest that there was a broad range of factors and circumstances influencing how people interacted with cinnabar. Why were non-elite members of the community, for example the person buried in Structure 10.074 at Valencina, so seriously exposed to a high-end commodity which at that site was only used in elite graves? What was their connection with cinnabar? Were they involved in the extraction (mining) or manipulation of the substance? Undoubtedly, a number of people must have been involved in the procurement and preparation of cinnabar and must have experienced some degree of exposure to it. Alternatively, some people may have used it in a variety of ways (body paint, consumption, etc.) but were not able to use it in their funerals. Indeed, some people appear to have been able to make funerary use of the red powder, although the analysis of their bones revealed no mercury exposure, as suggested at Cabeço Arruda and Paimogo I. Therefore, the patterns of interaction between people and cinnabar must have been complex and organised by numerous principles of varying nature, including, probably, biological (age and sex), social, ethnic and cultural. This topic will be further discussed below. An additional point of possible relevance is that at Valencina, the graves without cinnabar in which the highest THg levels are observed are collective (between two and nine individuals), whereas THg values observed in individual graves are rather low (between 0.19 and 1.54 µ/g). Were persons buried in individual graves not attached to larger kin or social groups? And, if that was the case, did they have less interaction with the red substance (or were they even ‘segregated’ from it)? Finally, there is an important element to consider in terms of the intra-site variation of mercury exposure at Valencina: time. As is shown graphically in Fig.9, with the exception of three slightly earlier features (structures 10.042–10.049, 10.071 and 10.044), all the Montelirio tholos and PP4-Montelirio individuals analysed here died within a relatively short period of time, between 2900 and 2650 BC (a full discussion of this chronology is available in García Sanjuán etal., in preparation). This is important because this ‘short’ chronology shows that both the remarkable use of cinnabar and high exposure to mercury this people experienced were part of relatively short-termed social and cultural dynamic, lasting between two and two and a half centuries. Cinnabar ‘flowed’ extensively during this period in Valencina, as it did in other contemporary sites of south-western Iberia as discussed above and was intensely present in social life, ritual performances and burial ceremonies. It was, in a way, a period of ‘cinnabar rush’. Cinnabar Use The next obvious question is: how did these high accumulations of mercury occur among the Valencina population? What were the environmental conditions and/ or cultural practices that led to what appears to be, on the basis of the currently available evidence, the most intense exposure to mercury in human history, with the 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… possible exception of Tokushima (Shikoku, Japan), during the sixth–seventh centuries AD (Yamada etal., 1995). Cinnabar is not frequent in nature, which makes it a raw material hard to procure, with a high cost of acquisition and a demonstrable history of use as a high-end commodity. Its bright red colour, of spectacular visual effect, afforded it a high symbolism as a sacred, magic and exclusive material, only accessible to the initiated or to the elites. In Structure 10.042–10.049 at Valencina, cinnabar was sprayed on the corridor, covering some perforated beads, the body of ‘The Ivory Lady’ (10.049), and some of the grave goods surrounding her (especially a large almond-rim type ceramic plate of large size placed at her back; García Sanjuán etal., 2019; Rogerio-Cande- lera etal., 2013). In the remarkable Montelirio monument, cinnabar had a pervasive presence. It was used to coat and paint the large-sized slate slabs that line both corridors and both chambers. Cinnabar-based pigments were sprayed abundantly in the LC, above the bodies and the grave goods, and in the hearth located at its centre; red pigment was also used, perhaps burned (Fernández Flores & García Sanjuán, 2016:108). Next to the unbaked-clay stela that presided over the LC, three small anthropomorphic figurines also made of unbaked clay had been painted with Fig. 9 Synthesis of total mercury levels (µg/g) over time in Valencina L.García Sanjuán et al. 1 3 cinnabar (Bueno Ramírez etal., 2016: 391). For all other Valencina features examined in this study, no cinnabar was reported with the only exception of Structure 10.074, where small amounts of it were noted. Undoubtedly, the Copper Age population of Valencina attached great symbolic and ritual significance to cinnabar. The individual from Structure 10.074 with very high THg values, who does not appear to be an elite member, may have been involved in the procurement or processing of the raw material. In addition, in Valencina cinnabar was strictly associated with death as no cinnabar has ever been identified in non-funerary features, unlike the site of Gramalote (Peru), dated to the 2nd millennium BC (Prieto etal., 2016), where cinnabar is found in both burial and domestic contexts. Considering the biocidal potential of mercury compounds,5 which may act against the proliferation of the micro-organisms involved in the decomposition of flesh; cinnabar may have also been aimed at slowing down or counteracting the decay of the bodies or even to achieve partial mummification (Delibes de Castro, 2000: 230; Cervini-Silva etal., 2013, 2018). Cinnabar does not occur naturally in the surroundings of Valencina and therefore was brought from further afield. The presence of this substance is consistent with the broad spectrum of foreign raw materials that made their way into burial and votive structures at the site (including abundant elephant ivory, a sperm-whale tooth, ostrich eggshells, various types of marine shells, flint, mylonite, rock crystal, amber, variscite and, probably, jadeite). Although initial studies provenanced Valencina cinnabar from the Almadén mining district (Hunt Ortiz & Hurtado Pérez, 2010; Hunt- Ortiz etal., 2011), located some 250km to the north, later research suggested a more complex picture in which establishing the precise origin of the substance is not straightforward (Rodríguez etal., 2020). In Perdigões, isotopic analysis suggests that cinnabar was brought from Almadén but also from one other unknown source (Emslie etal., 2015). Other cinnabar deposits exist in southern Spain, including Las Alpujarras (Tímar-Cástaras, Granada), about 350km to the east of Valencina, and Usagre (Badajoz), 120km to the north-west. There is evidence of the latter being exploited in the sixteenth century AD (Bleiberg, 1984). Unlike other minerals, such as flint, variscite, or copper, nothing is known about the mining of cinnabar in Neolithic and Copper Age Iberia. The long history of exploitation of the Almadén mercury mines (Gil Bautista, 2013, 2015; Zarzalejos Prieto etal., 2012, Zarzalejos Prieto, 2019) shows the deployment of a substantial workforce. In his twelfth century AD Description of Spain geographer Al-Idrisi mentioned over 1000 men working at the Almadén mines to obtain mercury and cinnabar, which were then shipped to all countries of the world (Gil Bautista, 2015: 61). After the mining, there was the problem of transportation from Almadén to Sevilla, across the Sierra Morena range. Since the sixteenth century onwards, the transportation of mercury to Sevilla for its shipment to Peru and Mexico is well-documented. 5 Their use as biocides has been banned by the European Union since 1 January 2021 because of their negative effects on the environment and human health (UE Regulation 2017/852 of the European Parliament and European Council directive 17 May 2017 on mercury use, which supersedes CE Regulation #1102/2008). 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… The distance was covered, preferably in April or May, either by oxen-pulled carts, which could carry weights of up to 460kg between 20 and 30days, or by mules in 8–10days (Gil Bautista, 2015:313). It has been suggested that the presence of several Bronze Age stelae along some of the pathways, tracks and roads used at that time point to their probable prehistoric origin (Gil Bautista, 2015:58). In fact, the spatial, GIS-based analysis of Late Neolithic and Copper Age megalithic monuments in Sierra Morena does indeed reveal a high degree of correlation with historic pathways (such as drovers), which in turn follow the layout of the most naturallytransitable (‘least-cost’) routes (Murrieta Flores, 2012; Murrieta Flores etal., 2011). The variability of THg levels in human bone and the variable occurrence of cinnabar with and without high THg bone levels suggests there were varying degrees of exposure to the substance. This in turn could be explained by diverse forms of interaction between people and the raw material, including its processing and application as well as its consumption. Among the former, several activities were needed: (i) repeated grinding and sieving of the material to obtain nanometric particles which could then be mixed with other red pigments, a labour intensive procedure; both in the Montelirio tholos (Hunt Ortiz & Hurtado Pérez, 2010: 124) and in Structure 10.042–10.049 (Rogerio-Candelera etal., 2013:286), cinnabar was found to have been mixed with more easily available iron oxides, in all probability with the aim of increasing the amount of material available for use; (ii) mixing of the red powder with natural binders (water, egg yolk, oils, etc.) to create liquid pigments that could be applied as paint on the walls of megalithic burials and on the bodies (presumably of both the dead and the living); (iii) spraying of powder on the burial areas. People working on the processing of the cinnabar ore into powder would have been constantly exposed to the involuntary inhalation and ingestion of the fine dust, which would have also fallen on foodstuffs and on the surfaces of surrounding living and working areas, likely causing it to enter the food chain (herbivores eating grass, etc.). Indeed, if cinnabar was more or less frequently used for bodily decoration, some people must have been exposed to mercury through the skin for substantial lengths of time or even constantly. Heating cinnabar powder, as is done even today in some regions of the world to elaborate medicinal drugs (Gerke, 2021), would have produced mercury vapours which, if inhaled, would have led to immediate health issues (alternations of the nervous system and sensory organs, etc.). If transported by the wind, these vapours could have transported mercury towards the surrounding environment, where it would have been easily deposited (Poulin & Gibb, 2008), leading to ulterior forms of exposure—see discussion below. In addition, it is likely that some ‘special’ people intentionally consumed cinnabar (or products that included it), either via inhalation of the vapours produced after heating it, or via direct ingestion (for example in liquid, or semi liquid form). There are several cross-cultural examples of the consumption of mercury and associated compounds. Cinnabar powder has been used until recently in Chinese and Indian traditional medicine and is still used today (Huang etal., 2007, 2012; Liu etal., 2008, 2018). Approximately 40 traditional Chinese drugs contain cinnabar (Liu etal., 2008, 2018: 3). In recent years, advances have been made in the understanding of the molecular-level mechanisms underlying the pharmacological and toxicological effects of cinnabar, which is of key importance to distinguish therapeutic from L.García Sanjuán et al. 1 3 toxic doses (Lu etal., 2020; Yang etal., 2020). In the Copper Age, the consumption of cinnabar by a group of initiated or special people would have been made on the belief that cinnabar had healing and/or magic properties. Indeed, it is quite possible that certain effects caused by the exposure to the substance (such as shakes, convulsions, loss of consciousness) that are akin to those verified in trance states were sought-after. As mentioned above, the group of women buried in the LC of Montelirio display some very specific biological and cultural traits suggesting they were religious specialists. However, even if some specific forms of use were restricted to a small group of people, at some point, cinnabar powder must have been, on account of its processing and manipulation, a relatively common, even pervasive occurrence at Valencina not just in the domain of the dead, but also in daily lives of the people living in and frequenting the site. Having said that, it is worth noting that at Valencina, no cinnabar powder has ever been recorded in non-funerary features. Environmental Exposure The discussion made above suggests two major forms of interaction with cinnabar at Copper Age Valencina: processing and consumption. By their very nature, both of them must have affected a small number of people. And yet, the presence of mercury in human bone is quite pervasive, to which it must be added that it is also found in animal bone and in the only soil sample analysed to date. This suggests a possible third vector of interaction: the environment. Today, mercury occurs naturally in the environment, mainly on account of volcanic eruptions and because of several thousand years of human activity (Sundseth etal., 2017). Mercury presence is also quite persistent. Most of the mercury found in Earth’s atmosphere today (up to 60%) is anthropogenic Hg ‘inherited’ from reemissions from surface deposits, while natural emissions and primary anthropogenic emissions account for 13% and 27%, respectively (Amos etal., 2013). Studies carried out in Iceland show the accumulation of mercury in the bones of individuals living in volcanic environments (Walser etal., 2019). A recent study (Freire etal., 2010) found that the infant population of present-day Granada (Spain) shows higher mercury exposure (1–2µg/g MeHg in hair) than populations from more industrially polluted areas of northern Spain and Europe. The explanation for this, it has been suggested, could lay in fish consumption through the incorporation of environmental mercury to water and subsequent entry in the trophic chain (Freire etal., 2010: 100–101). Upon its deposition from the atmosphere, aquatic organisms biotransform environmental mercury into organic mercury (methylmercury, MeHg), a more easily available form that is incorporated into the trophic chain through water and food, mainly fish and molluscs. Most organic mercury compounds are easily absorbed through the lungs, gastrointestinal tract or skin. Exposure to MeHg can have various types of effects on the central nervous system, including motor and sensory alterations, visual impairment, and migraines, some of which may disappear if the exposure is discontinued. Severe effects include lasting cognitive damage: a linear 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… relationship has been established between the loss of 0.18 IQ points and a 1µg/g increase of mercury in hair (Axelrad etal., 2007). Obviously, in the Copper Age there was very little or no history of previous anthropogenic mercury in the environment, so one relevant question is, were there significant levels of naturally occurring environmental mercury in the Valencina region during the 3rd millennium BC? Isotopic data suggest that the diet of the Valencina population studied here was not rich in marine resources (Fontanals Coll etal., 2016: 446). Basically, the same applies to other Copper Age sites for which data are available (Beck etal., 2018:37). Although the real extent of fish and seafood consumption during the Copper Age is yet to be established through more robust sampling and a broader examination at the evidence, mercury intake through that vector appears to have been low. For the four individuals of Perdigões and Sobreira da Cima for whom the proportion of MeHg (CH3Hg) with regards to THg could be established, the resulting values were lower than 0.05% (Emslie etal., 2015:6). This suggests that virtually all measured mercury is in inorganic form, again ruling out large intakes through diet. Other sources of environmental mercury must be considered. Mining unrelated to cinnabar (for example copper) was important in the Copper Age. It has been suggested that early copper mining in the Huelva ‘pyritic belt’, barely 30km to the West of Valencina as the crow flies, may have caused significant environmental pollution at distances of up to 100km from the mines (Leblanc etal., 2000). Long-term atmospheric data do not suggest that lead levels in the Copper Age were significantly higher than those of the pre-metallurgical era (García-Alix etal., 2013), but copper mining and smelting activity may have caused some small-scale impact on the environment in which the Valencina population was living. Could the numerous cases of medium-to-high levels of THg in human bone at Valencina be explained through environmental mercury? This could be potentially relevant for individuals with bone concentrations around 1µg/g in whose burials no remains of cinnabar were observed. This explanation could apply to the Neolithic individual found in the Montelirio mound (pre-dating the construction of the tholos by almost 2000years), who yielded THg values of 4.32µg/g, or to the Roman-time burials of the PP4-Montelirio sector, with average THg values of 0.32µg/g and a maximum of 1.32µg/g. Indeed, environmental mercury was invoked to explain the presence of medium-to-high THg levels at the necropolis of La Lanzada (Galicia) (Álvarez-Fernández etal., 2020; López-Costas etal., 2020). Environmental mercury, whether because of natural occurrence, mining of other metals in the ‘pyritic belt’ or deposition caused in the surrounding area of the site by the processing of cinnabar, could also explain the medium-to-high THg levels found in animal bones at Valencina, which are higher than the only comparable sample, dating to the Bronze Age (Logemann etal., 1994), as well as those found in soil samples from the Pabellón Cubierto (14.5µg/g) where no cinnabar was reported. Future studies will have to place more emphasis on animal and soil samples to clarify the extent of mercury in the environment of 3rd-millennium BC Valencina. Altogether, although environmental mercury may have played a part in the medium-to-low mercury levels found in the bones of most of the Valencina Copper Age population, the highest levels must have occurred in connection with specific cultural practices as described above. L.García Sanjuán et al. 1 3 Díaz-Guardamino, M.; Wheatley, D. W.; Williams, E. F. and Garrido Cordero, J. A. (2016): “Los textiles elaborados con cuentas perforadas de Montelirio”, In Fernández Flores, Á.; García Sanjuán, L. and Díaz-Zorita Bonilla, M. (eds.): Montelirio: Un Gran Monumento Megalítico de la Edad del Cobre, 345–364. Sevilla, Junta de Andalucía Domingo, I., García-Borja, P., & Roldán, C. (2012). Identification, processing, and use of red pigments (hematite and cinnabar) in the Valencian Early Neolithic (Spain). Archaeometry, 54, 868–892. https:// doi. org/ 10. 1111/j. 1475- 4754. 2011. 00650.x Domínguez Bella, S., & Morata Céspedes, D. (1995). Aplicación de las técnicas mineralógicas y petrológicas a la arqueometría. Estudio de materiales del dolmen de Alberite (Villamartín, Cádiz). Zephyrus, 48, 129–142. Domínguez Bella, S. (2010). plicaciones de las técnicas experimentales y la mineralogía a la Arqueometría. Los pigmentos de cinabrio del dolmen de Alberite I (Villamartín, Cádiz). In S. Domínguez- Bella, J. Ramos Muñoz, J. M. Gutiérrez López, & M. Pérez Rodríguez (Eds.), Minerales y Rocas en las Sociedades de la Prehistoria (pp. 235–244). Cádiz: Universidad de Cádiz. Driscoll, C. T., Mason, R. P., Chan, H. M., Jacob, D. J., & Pirrone, N. (2013). Mercury as a global pollutant: Sources, pathways, and effects. Environmental Science & Technology, 47, 4967–4983. EFSA Panel on Contaminants in the Food Chain (CONTAM). (2012). Scientific opinion on the risk for public health related to the presence of mercury and methylmercury in food. Efsa Journal, 10(12), 2985. https:// doi. org/ 10. 2903/j. efsa. 2012. 2985 Emslie, S. D., Alderman, A., McKenzie, A., Brasso, R., Taylor, A., Molina Moreno, M., Cambra-Moo, O., Martín, A. G., Silva, A. M., Valera, A., & Vijande Vila, E. (2019). Mercury in archaeological human bone: Biogenic or diagenetic? Journal of Archaeological Science, 108, 104969. https:// doi. org/ 10. 1016/j. jas. 2019. 05. 005 Emslie, S. D., Silva, A. M., Valera, A., Vijande Vila, E., Melo, L., Curate, F., Fidalgo, D., Inácio, N., Molina Moreno, M., Cambra-Moo, O., González Martín, A., Barroso-Bermejo, R., Montero Artús, R., & García Sanjuán, L. (2022). The use and abuse of cinnabar in Late Neolithic and Copper Age Iberia. International Journal of Osteoarchaeology, 32(1), 202–214. https:// doi. org/ 10. 1002/ oa. 3056 Emslie, S.D., Brasso, R., Patterson, W., Valera, A.C., McKenzie, A., Silva, A.M., Gleason, J.D., Blum, J.D. (2015). Chronic mercury exposure in Late Neolithic/Chalcolithic populations in Portugal from the cultural use of cinnabar. Scientific Reports 5 https:// doi. org/ 10. 1038/ srep1 4679. Emslie, S.D., McKenzie, A.C., Shaller, H.E. (2016). Análisis de mercurio de los restos humanos del Tholos de Montelirio. In: Fernández Flores, A., García Sanjuán, L., Díaz Zorita Bonilla, M. (Eds.), Montelirio Un Gran Monumento Megalítico de la Edad del Cobre, Arqueología Monografías. Junta de Andalucía, Spain, pp. 449–454 European Commission. (2017). Science for Environment Policy, Tackling mercury pollution in the EU and worldwide. In-depth Report 15 produced for the European Commission, DG Environment by the Science Communication Unit, UWE, Bristol. -- http:// ec. europa. eu/ scien ceenvir onmentpolicy´ Fernández Flores, A., García Sanjuán, L. (2016). Arquitectura, estratigrafía y depósitos del tholos de Montelirio. In A. Fernández Flores, L. García Sanjuán, & M. Díaz-Zorita Bonilla (Eds.), Montelirio: Un Gran Monumento Megalítico de la Edad del Cobre (pp. 79–142). Seville: Junta de Andalucía. Fernández Flores, A., García Sanjuán, L., Díaz-Zorita, M. (2016). Montelirio: Un Gran Monumento Megalítico de la Edad del Cobre. Junta de Andalucía. Sevilla. Fontanals Coll, M., Díaz-Zorita Bonilla, M., Subirá de Galdácano, M.E. (2016). Análisis de isótopos estables de los restos humanos de Montelirio. In A. Fernández Flores, L. García Sanjuán & M. Díaz‐Zorita Bonilla (Eds.), Montelirio: Un Gran Monumento Megalítico de la Edad del Cobre Seville: Junta de Andalucía, 443–454. Fornaciari, G., Marinozzi, S., Cazzaniga, V., Giufrà, V., Picchi, M. S., Giusiani, M., & Masetti, M. (2011). The use of mercury against pediculosis in the Renaissance: The case of Ferdinand II of Aragon, King of Naples, 1467–96. Medical History, 55, 109–115. https:// doi. org/ 10. 1017/ s0025 72730 00060 74 Fornaciari, A., Chericoni, S., Stefanelli, F., Fornaciari, G., & Giufra, V. (2022). Renaissance mercurial therapy in the mummies of Saint Domenico Maggiore in Naples: A palaeopathological and palaeotoxicological approach. Archaeological and Anthropological Sciences, 14, 47. https:// doi. org/ 10. 1007/ s12520- 022- 01512-7 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… Freire, C., Ramos, R., Lopez-Espinosa, M. J., Díez, S., Vioque, J., Ballester, F., & Fernández, M. F. (2010). Hair mercury levels, fish consumption, and cognitive development in preschool children from Granada, Spain. Environmental Research, 110, 96–104. https:// doi. org/ 10. 1016/j. envres. 2009. 10. 005 Gajic-Kvascev, M., Stojanovich, M. M., Smit, Z., Kanterelou, V., Karydas, A., Sljivar, D., Milovanovic, D., & Andric, V. (2012). New evidence for the use of cinnabar as a colouring pigment in the Vinca culture. Journal Archaeological Science, 39, 1025–1033. García Sanjuán, L., Luciañez Triviño, M., Schuhmacher, T., Wheatley, D. W., & Banerjee, A. (2013). Ivory craftsmanship, trade and social significance in the southern Iberian Copper Age: The evidence from the PP4-Montelirio sector of Valencina de la Concepción (Seville, Spain). European Journal of Archaeology, 16(4), 610–635. García Sanjuán, L., Scarre, C., & Wheatley, D. (2017). The mega-site of Valencina de la Concepción (Seville, Spain): Debating settlement form, monumentality and aggregation in southern Iberian Copper Age societies. Journal of World Prehistory, 30(3), 239–257. García Sanjuán, L., Vargas Jiménez, J. M., Cáceres Puro, L., Costa Caramé, M. E., Díaz-Guardamino- Uribe, M., Díaz-Zorita Bonilla, M., Fernández Flores, Á., Hurtado Pérez, V., López Aldana, P. M., Méndez Izquierdo, E., Pajuelo Pando, A., Rodríguez Vidal, J., Wheatley, D., Delgado-Huertas, A., Dunbar, E., Mora González, A., Bronk Ramsey, C., Bayliss, A., Beavan, N., … Whittle, A. (2018a). Assembling the dead, gathering the living: Radiocarbon dating and Bayesian modelling for Copper Age Valencina de la Concepción (Sevilla, Spain). Journal of World Prehistory, 31(2), 179–313. García Sanjuán, L., Luciañez Triviño, M., & Cintas-Peña, M. (2018b). Ivory, elites and lineages in Copper Age Iberia: Exploring the wider significance of the Montelirio tomb. Madrider Mitteilungen, 59, 23–65. García Sanjuán, L., Fernández Flores, A., Díaz‐Zorita Bonilla, M. (2016). Montelirio. Valoración e interpretación de una tumba excepcional. In A. Fernández Flores, L. García Sanjuán, M. Díaz‐Zorita Bonilla (Eds.), Montelirio: un Gran Monumento Megalítico de la Edad del Cobre (pp. 503–553). Seville: Junta de Andalucía. García Sanjuán, L.; Cintas-Peña, M.; Díaz-Guardamino, M.; Escudero Carrillo, J.; Luciañez Triviño, M.; Mora Molina, C. and Robles Carrasco, S. (2019): “Burial practices and social hierarchisation in Copper Age Southern Spain: Analysing tomb 10.042–10.049 of Valencina de la Concepción (Seville, Spain)”, In Müller, J.; Hinz, M. and Wunderlich, M. (eds): Megaliths, societies, landscapes. Early Monumentality and Social Differentiation in Neolithic Europe, 1005–1037. Frühe Monumentalität und soziale Differenzierung 18/III, Bonn, Habelt. García Sanjuán, L.; Sánchez Díaz, F. and Morell Rovira, L. (2022): “The ‘Megalithisation’ of Iberia: A spatio-temporal model”, L’ Anthropologie. García Sanjuán, L.; Sánchez Díaz, F. and Cintas-Peña, M. (in preparation): “The time and tempo of burial practices at Valencina, c. 2850–2650 cal BC: Bayesian and spatial modelling” García-Alix, A., Jiménez Espejo, F. J., Lozano Rodríguez, J. A., Jiménez-Moreno, G., Martínez-Ruiz, F., García Sanjuán, L., Aranda Jiménez, G., García Alfonso, E., Ruiz-Puertas, G., & Anderson, S. (2013). Anthropogenic impact and lead pollution throughout the Holocene in Southern Iberia. Science of the Total Environment, 449, 451–460. Garrido-Pena, R.; Flores, R. and Herrero‐Corral, A. M. (2019). Las Sepulturas Campaniformes de Humanejos (Parla, Madrid). Dirección General Patrimonio Cultural Comunidad de Madrid. Gavilán Ceballos, B., Mas i Cornellá, M., Rodríguez, Y., Solís, M., & Garrido, E. (2020). Hallazgos de cinabrio en contextos del Neolítico cordobés: la Cueva de los Murciélagos (Zuheros) y el Dolmen de Casas de Don Pedro (Belmez). In M. Zarzalejos Prieto, P. Hevia Gómez, & L. Mansilla Plaza (Eds.), El Oro Rojo en la Antigüedad. Perspectivas de Investigación sobre los Usos y Aplicaciones del Cinabrio entre la Prehistoria y el Fin del Mundo Antiguo (pp. 161–186). Madrid: UNED. Gaydarska, B., & Chapman, J. (2022). Megasites in prehistoric Europe. Cambridge, Cambridge University Press. Gerke, B. (2021). Taming the poisonous. Mercury, Toxicity, and Safety in Tibetan Medical Practice. Heidelberg Studies on Transculturality, Vol. 7. Heidelberg University Publishing (heiUP). https:// doi. org/ 10. 17885/ heiup. 746 Gil Bautista, R. (2009). Medicina y minería en el Almadén del siglo XVIII. Joseph Parés y Franqués (Mataró, 1720-Almadén, 1798). Revista de Historia Moderna, 27, 333–362. https:// doi. org/ 10. 14198/ RHM20 09. 27. 14 L.García Sanjuán et al. 1 3 Gil Bautista, R. (2013). Almadén del Azogue. Una Villa Minera en el Siglo XVIII, Puertollano (Ciudad Real). Gil Bautista, R. (2015). Las Minas de Almadén en la Edad Moderna. Universidad de Alicante. Gleba, M., Bretones-García, M. D., Cimarelli, C., Vera-Rodríguez, J. C., & Martínez-Sánchez, R. M. (2021). Multidisciplinary investigation reveals the earliest textiles and cinnabar-coloured cloth in Iberian Peninsula. Scientific Reports, 11, Article Number 21918. https:// doi. org/ 10. 1038/ s41598- 021- 01349-5 Gliozzo, E. (2021). Pigments — Mercury-based red (cinnabar-vermilion) and white (calomel) and their degradation products. Archaeological and Anthropological Sciences, 13, 210. https:// doi. org/ 10. 1007/ s12520- 021- 01402-4 González Tascón, I.; Romero Muñoz, D. & Saénz Sanz, A. (1996). Los caminos del mercurio. De Almadén a Nueva España, in: Actas del II Congreso Internacional de Caminería Hispánica, Vol. II, 683–692. Guadalajara, Junta de Castilla La Mancha. González-Cruz, A. (2011). La Reina Roja. Proyecto Arqueológico Palenque, Chiapas, México. Instituto Nacional de Antropología e Historia (INAH), México D.F. Goren, Y., Goring-Morris, A. N., & Segal, I. (2001). The technology of skull modelling in the Pre-Pot- tery Neolithic B (PPNB): Regional variability, the relation of technology and iconography and their archaeological implications. Journal of Archaeological Science, 28, 671–690. https:// doi. org/ 10. 1006/ jasc. 1999. 0573 Guilaine, J. (2018). Siret Smile. Antiquity, 92(365), 1247–1259. Guiral Pelegrín, C. & Íñiguez Berrozpe, L. (2020). El cinabrio en la pintura romana de Hispania, In: Zarzalejos Prieto, M.; Hevia Gómez, P. and Mansilla Plaza, L. (eds.): El Oro Rojo en la Antigüedad. Perspectivas de Investigación sobre los Usos y Aplicaciones del Cinabrio entre la Prehistoria y el Fin del Mundo Antiguo, 337–372. Madrid, UNED. Halffman, C.M. (2009). Bone as a biomarker of mercury exposure in prehistoric arctic human populations: Initial method validation using animal models. Thesis, University of Alaska, Fairbanks. http:// hdl. handle. net/ 11122/ 9008 Hernández Sobrino, R. (2007). Los Mineros del Azogue. Ciudad Real Higueras, P., Esbrí, J. M., González Corrochano, B., López Berdonces, M. A., García Noguero, E. M., Martínez Coronado, A., Llanos Lazcano, W., & Lorenzo Álvarez, S. (2012). Consideraciones ambientales sobre el mercurio en el distrito minero de Almadén (Ciudad Real). De Re Metallica, 19, 53–65. Huang, C. F., Liu, S. H., & Lin-Shiau, S. Y. (2007). Neurotoxicological effects of cinnabar (a Chinese mineral medicine, HgS) in mice. Toxicology and Applied Pharmacology, 224, 192–201. Huang C.F., Hsu C.J., Liu S.H. & Lin-Shiau S.Y. (2012). Exposure to low dose of cinnabar (a naturally occurring mercuric sulfide (HgS)) caused neurotoxicological effects in offspring mice. Journal of Biomedicine and Biotechnology 2012 https:// doi. org/ 10. 1155/ 2012/ 254582. Hunt Ortiz, M.A. & Hurtado Pérez, V.M. (2010). Pigmentos de sulfuro de mercurio “cinabrio” en contextos funerarios de época calcolítica en el sur de la Península Ibérica: investigaciones sobre el uso, depósitos minerales explotados y redes de distribución a través de la caracterización composicional e isotópica. In Sáiz Carrasco, M.E., López Romero, R., Cano Díaz-Tendero, M.A., Calvo García, J.C. (eds.), Actas del VIII Congreso Ibérico de Arqueometría: 123–131. Seminario de Arqueología y Etnología Turolense, Teruel. Hunt‐Ortiz, M.A., Consuegra‐Rodríguez, S., Díaz del Río‐Español, P., Hurtado‐Pérez, V.M., & Montero‐ Ruiz, I. (2011). Neolithic and Chalcolithic—VI to III millennia BC—use of cinnabar (HgS) in the Iberian Peninsula: Analytical identification and lead isotope data for an early mineral exploitation of the Almadén (Ciudad Real, Spain) mining district. In J. E. Ortiz, I. Rábano & L. F. Mazadiego (eds.), History of Research in Mineral Resources, Cuadernos del Museo Geominero 13: 3–14. Instituto Geológico y Minero de España. Inácio, N.; Nocete Calvo, F.; Nieto, J. M.; Sáez, R.; Rodríguez Bayona, M. and Peramo, A. (2013) A presença de cinabrio em contextos megalíticos do Sul de Portugal, In: Jiménez Ávila, J.; Bustamante, M. and García Cabezas, M. (eds.): Actas del VI Encuentro de Arqueología del Suroeste Peninsular (Villafranca de los Barros, Badajoz, 4–6 de Ocubre de 2012), 417–430. Villafranca de los Barros, Ayuntamiento de Villafranca de los Barros. ICSC (International Chemical Safety Cards). (2019). 0056, 0978, 0979, 0980, 0981, 0982 and 0984 on a number of mercury compounds. Geneva: World Health Organization and International Labour Organization. 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… Kabata-Pendias, A. (2010). Trace elements in soils and plants. Taylor & Francis Group, Fourth Edition, ProQuest Ebook Central, https:// ebook centr al. proqu est. com/ lib/ uses/ detail. action? docID= 14466 31. Kobal, A. B., Horvat, M., Prezelj, M., Briski, A. S., Krsnik, M., Dizdarevic, T., Mazej, D., Falnoga, I., Stibilj, V., Arneric, N., Kobal, D., & Osredkar, J. (2004). The impact of long-term past exposure to elemental mercury on antioxidative capacity and lipid peroxidation in mercury miners. Journal of Trace Elements in Medicine and Biology, 17(4), 261–274. Leblanc, M., Morales, J. A., Borrego, J., & Elbaz-Poulichet, F. (2000). 4,500-year-old mining pollution in Southwestern Spain: Long-term implications for modern mining pollutions. Economic Geology, 95(3), 655–662. https:// doi. org/ 10. 2113/ gseco ngeo. 95.3. 655 Liesau, C., Aparicio, M.T., Araujo, R., Llorente, L., Morales, A. (2014). La fauna del Sector PP4-Monte- lirio del yacimiento prehistórico de Valencina de la Concepción (Sevilla). Economía y Simbolismo de los animales en una comunidad del III milenio. Menga: Revista de Prehistoria de Andalucía 4, pp. 69–102. Liesau, C., Blasco-Bosqued, C. & Ríos Mendoza, P. (2020). El cinabrio: un color para las élites. Su presencia en los contextos funerarios campaniformes en la región, In: Zarzalejos Prieto, M.; Hevia Gómez, P. and Mansilla Plaza, L. (eds.): El Oro Rojo en la Antigüedad. Perspectivas de Investigación sobre los Usos y Aplicaciones del Cinabrio entre la Prehistoria y el Fin del Mundo Antiguo, 201–224. Madrid. UNED. Liu, J., Shi, J. Z., Yu, L.-M., Goyer, R. A., & Waalkes, M. P. (2008). Mercury in traditional medicines: Is cinnabar toxicologically similar to common mercurials? Experimental Biolology and Medicine, 233(7), 810–817. https:// doi. org/ 10. 3181/ 0712- MR- 336 Liu, J., Wei, L. X., Wangc, Q., Lu, Y.-F., Zhang, F., Shi, J.-Z., Li, C., & Cheriane, M. G. (2018). A review of cinnabar (HgS) and/or realgar (As4S4)-containing traditional medicines. Journal of Ethnopharmacology, 210, 340–350. Logemann, E., Kalkbrenner, G., Krützfeldt, B., Schüle, W. (1994). Mercury in bones in domestic animals: Evidence of prehistoric transhumance. In Vina Spiehler (eds.) Proceedings of the 1994 Joint Tiaft/Soft International Meeting. Bridging the World of Forensic Toxicology: 163–171. Newport Beach, CA. Lombardo, D., Colard, T., Bandiera, P., etal. (2022). Dental developmental defects due to mercurial treatment in a child from sixteenth-century Alghero (Sardinia, Italy). Archaeological and Anthropological Sciences, 14, 193. https:// doi. org/ 10. 1007/ s12520- 022- 01657-5 Lominchar, M.A., Sierra, M.J., Rodríguez, J., Millán, R. (2010). Estudio del comportamiento y distribución del mercurio presente en muestras de suelo recogidas en la Ribera del Río Valdeazogues. Informes Técnicos Ciemat 1215, Editorial CIEMAT, Madrid. López Padilla, J. A., De Miguel Ibáñez, M. P., Arnay de la Rosa, M., Galindo Martín, L., Roldán García, C., & Murcia Mascarós, S. (2012). Ocre y cinabrio en el registro funerario del Argar. Trabajos de Prehistoria, 69(2), 273–292. https:// doi. org/ 10. 3989/ tp. 2012. 12092 López-Costas, O., Kylander, M., Mattielli, N., Álvarez-Fernández, N., Pérez-Rodríguez, M., Mighall, T., Bindler, R., & Martínez Cortizas, A. (2020). Human bones tell the story of atmospheric mercury and lead exposure at the edge of Roman World. Science of The Total Environment, 710, 136319. https:// doi. org/ 10. 1016/j. scito tenv. 2019. 136319 Lu, Y.-T., Qi, W.-Z., Wang, S., Song, X.-N., Yang, D.-Y., Song, M., & Hang, T.-J. (2020). Toxicity and risk assessment of mercury exposures from cinnabar and Baizi Yangxin Pills based on pharmacokinetic and tissue distribution studies. Journal of Ethnopharmacology, 250, 112489. https:// doi. org/ 10. 1016/j. jep. 2019. 112489 Luciañez Triviño, M., García Sanjuán, L., & Schuhmacher, T. (2022). Crafting idiosyncrasies. Early Social Complexity, Ivory and Identity-Making in Copper Age Iberia, Cambridge Archaeological Journal, 32(1), 23–60. Mahdihassan, S. (1987). History of cinnabar as drug, the natural substance and the synthetic product. Indian Journal of History of Sciences, 22(1), 63–70. Martín Gil, J., Martín Gil, F. J., Delibes De Castro, G., Zapatero Magdaleno, P., & Sarabia Herrero, J. (1995). The first known use of vermillion. Cellular and Molecular Life Sciences, 51, 759–761. Martínez, M. J., Gavilán, B., Barrios, J., & Montealegre, L. (1999). Materias primas colorants en Murciélago de Zuheros (Córdoba): Caracterización y procedencia, in Actes del II Congrés del Neolític a la Península Ibèrica: Saguntum (PLAV) (eds. J. Bernabeu and T. Orozco). Extra, II, 111–116. Martínez-Sevilla, F., García Sanjuán, L., Lozano Rodríguez, J. A., Martínez Jordán, J. M., Scarre, C., Vargas Jiménez, J. M., Pajuelo Pando, A., & López Aldana, P. (2020). A new perspective on L.García Sanjuán et al. 1 3 Copper Age technology, economy and settlement. Grinding tools at the Valencina Mega-Site. Journal of World Prehistory, 33(4), 513–559. McDowell, M. A., Dillon, C. F., Osterloh, J., Bolger, P. M., Pellizzari, E., Fernando, R., de Oca, M. R., Schober, S. E., Sinks, T., Jones, R. L., & Mahaffe, K. R. (2004). Hair mercury levels in US children and women of childbearing age: Reference range data from NHANES 1999–2000. Environmental Health Perspectives, 112(11), 1165–1171. Menéndez Navarro, A. (2012). Trabajo, enfermedad y asistencia en las minas de Almadén (Ciudad Real), Siglos XVI-XX. De Re Metallica, 19, 95–102. Menéndez Navarro, A. (1993). La literatura sobre aspectos sanitarios del trabajo en las minas de mercurio de Almadén, 1755 – 1924. Medicina e Historia 48. Barcelona. Miculescu, F., Ciocan, L. T., Miculescu, M., Ernuteanu, A., Antonia, I., Matei, E., & Pencea, I. (2012). A study on trace elements concentration in bone particles by XRF análisis. Solid State Phenomena, 188, 37–40. https:// doi. org/ 10. 4028/ www. scien tific. net/ ssp. 188. 37 Mioč, U. B., Colomban, P., Sagon, G., Stojanović, M., & Rosić, A. (2004). Ochre decor and cinnabar residues in Neolithic pottery from Vinča, Serbia. Journal of Raman Spectroscopy, 35, 843–846. https:// doi. org/ 10. 1002/ jrs. 1221 Molina, L. (2004). Sobre el estanque de mercurio de Medina Azahara. Al Qantara, 25(2), 329–333. Mora Molina, C., García Sanjuan, L., Peinado Cucarella, J., & Wheatley, D. (2013). Las estructuras de la Edad del Cobre del Sector PP4-Montelirio del sitio arqueológico de Valencina de la Concepción - Castilleja de Guzmán (Sevilla). In L. García Sanjuan, J. M. Vargas Jiménez, V. Hurtado Pérez, T. Ruiz Moreno, & R. Cruz-Auñón Briones (Eds.), El Asentamiento Prehistórico de Valencina de la Concepción (Sevilla): Investigación y Tutela en el 150 Aniversario del Descubrimiento de La Pastora (pp. 261–279). Universidad de Sevilla. Murillo-Barroso, M., Costa Caramé, M. E., Díaz-Guardamino Uribe, M., García Sanjuán, L., & Mora Molina, C. (2015). A reappraisal of Iberian Copper Age goldwork: Craftmanship, symbolism and art in a non-funerary golden-sheet from Valencina de la Concepción. Cambridge Archaeological Journal, 25(3), 565–596. Murrieta Flores, P. A. (2012). Entendiendo la movilidad humana mediante tecnologías espaciales: El papel de las áreas naturales de tránsito en el Suroeste de la Península Ibérica durante la Prehistoria Reciente. Trabajos De Prehistoria, 69, 103–122. Murrieta Flores, P. A., Wheatley, D. W., & García Sanjuán, L. (2011). Movilidad y vías de paso en los paisajes prehistóricos: megalitos y vías pecuarias en Almadén de la Plata (Sevilla, España). In Tecnologías de Información Geográfica y Análisis Arqueológico del Territorio. Actas del V Symposio Internacional Arqueología de Mérida (Mérida, 7–10 de noviembre de 2007), Mayoral Herrera V & Celestino Pérez S (eds.). Anejos de AEsPa 59, Mérida: 412–423. Needham, J. (1976). Science and Civilisation in China: (Vol. 5). Cambridge University Press. Nocete Calvo, F., Sáez Ramos, R., Nieto Liñán, J. M., Cruz-Auñón Briones, R., Cabrero García, R., Alex, E., & Rodríguez Bayona, M. (2005). Circulation of silicified oolitic limestone blades in South- Iberia (Spain and Portugal) during the third millennium B.C.: An expression of a core/periphery framework. Journal of Anthropological Archaeology, 24(1), 62–81. Nocete Calvo, F., Queipo De Llano, G., Sáez Ramos, R., Nieto Liñán, J. M., Inácio, N., Rodríguez Bayona, M. R., Peramo, A., Vargas Jiménez, J. M., Cruz-Auñón Briones, R., Gil-Ibarguchi, J. I., & Santos, J. F. (2008). The smelting quarter of Valencina de la Concepción (Seville, Spain): The specialised copper industry in a political centre of the Guadalquivir valley during the Third Millennium BC (2750–2500 BC). Journal of Archaeological Science, 35, 717–732. Nocete Calvo, F., Vargas Jiménez, J. M., Schuhmacher, T. X., Banerjee, A., & Dindorf, W. (2013). The ivory workshop of Valencina de la Concepción (Seville, Spain) and the identification of ivory from Asian elephant on the Iberian Peninsula in the first half of the 3rd millennium BC. Journal of Archaeological Science, 40, 1579–1592. Nocete Calvo, F., Saez Ramos, R., Rodríguez Bayona, M., Nieto Liñán, J. M., Peramo, A., López, P., Gil- Ibarguchi, J. I., Inacio, N., García, S., & Rodríguez, J. (2014). Gold in the southwest of the Iberian Peninsula during the 3rd millennium BC. Journal of Archaeological Science, 41, 691–704. Ochoa-Lugo, M., Cervini-Silva, J., Muñoz, M. D., Palacios, E., Pérez-Ramirez, G., Ramos-Cruz, E., Montoya, A., & Romano-Pacheco, A. (2017). The effect of depositional conditions on mineral transformation, chemical composition, and preservation of organic material in archaeological Hgenriched bone remains, Journal of Archaeological Science: Reports, 15, 2352–2409. https:// doi. org/ 10. 1016/j. jasrep. 2017. 07. 015 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… Panova, T. D., Dmitriev, A. Y., Borzakov, S. B., etal. (2018). Analysis of arsenic and mercury content in human remains of the 16th and 17th centuries from Moscow Kremlin necropolises by neutron activation analysis at the IREN facility and the IBR-2 reactor FLNP JINR. Physics of Particles and Nuclei Letters, 15, 127–134. https:// doi. org/ 10. 1134/ S1547 47711 80101 32 Park, J.-D., & Zheng, W. (2012). Human exposure and health effects of inorganic and elemental mercury. Journal of Preventive Medicine & Public Health, 45, 344–352. https:// doi. org/ 10. 3961/ jpmph. 2012. 45.6. 344. ISSN 1975-8375. Parke, H. W. (1977). Dodona. In N. G. L. Hammond & H. H. Scullard (Eds.), The Oxford Classical Dictionary (p. 358). Oxford University Press. Pecero Espín, J.C. (2016). Caracterización antropológica de los restos óseos humanos del Tholos del Montelirio. In A. Fernández Flores, L. García Sanjuán, M., Díaz‐Zorita Bonilla (Eds.), Montelirio: Un Gran Monumento Megalítico de la Edad del Cobre (pp. 409–442). Seville: Junta de Andalucía. Poulin J & Gibb H. (2008). Mercurio: Evaluación de la carga de morbilidad ambiental a nivel nacional y local. Prüss-Üstün (ed.) A. Organización Mundial de la Salud, Ginebra, OMS, Serie Carga de Morbilidad Ambiental, n.º 16. Prieto, G., Wright, V., Burger, R. L., Cooke, C. A., Zeballos-Velasquez, E. L., Watanave, A., Suchomel, M. R., & Suescun, L. (2016). The source, processing and use of red pigment based on hematite and cinnabar at Gramalote, an early Initial Period (1500–1200 cal. B.C.) maritime community, north coast of Peru. Journal of Archaeological Science: Reports, 5, 45–60. Rasmussen, K. L., Boldsen, J. L., Kristensen, H. K., Skytte, L., Hansen, K. L., Mølholma, L., Grootes, P. M., Nadeau, M. J., & Eriksen, K. M. F. (2008). Mercury levels in Danish Medieval human bones. Journal of Archaeological Science, 35, 2295–2306. Rasmussen, K. L., Kucera, J., Skytte, L., Kameník, J., Havránek, V., Smolík, J., Velemínský, P., Lynnerup, N., Bruzek, J., & Vellev, J. (2012). Was he murdered or was he not? Part I: Analyses of mercury in the remains of Tycho Brahe. Archaeometry, 55(6), 1187–1195. https:// doi. org/ 10. 1111/j. 1475- 4754. 2012. 00729 Rasmussen, K. L., Skytte, L., Pilekær, C., Lauritsen, A., Boldsen, J. L., Leth, P. M., & Thomsen, P. O. (2013a). The distribution of mercury and other trace elements in the bones of two human individuals from medieval Denmark – The chemical life history hypothesis. Heritage Science, 1, 10. https:// doi. org/ 10. 1186/ 2050- 7445-1- 10 Rasmussen, K. L., Skytte, L., Ramseyer, N., & Boldsen, J. L. (2013b). Mercury in soil surrounding medieval human skeletons. Heritage Science, 1, 16. https:// doi. org/ 10. 1186/ 2050- 7445-1- 16 Rasmussen, K. L., Skytte, L., Jensen, A. J., & Boldsen, J. L. (2015). Comparison of mercury and lead levels in the bones of rural and urban populations in Southern Denmark and Northern Germany during the Middle Ages. Journal of Archaeological Science: Reports, 3, 358–370. https:// doi. org/ 10. 1016/j. jasrep. 2015. 06. 021 Rasmussen, K. L., Milner, G., Skytte, L., Lynnerup, N., Thomsen, J. L., & Boldsen, J. L. (2019). Mapping diagenesis in archaeological human bones. Heritage Science, 7, 41. https:// doi. org/ 10. 1186/ s40494- 019- 0285-7 Rick, J. W. (2017). The nature of ritual space at Chavín de Huántar. In S. A. Rosenfeld & S. L. Bautista (Eds.), Rituals of the Past: Prehispanic and Colonial Case Studies in Andean Archaeology (pp. 21–50). Denver: University Press of Colorado. Riley, D. M., Newby, C. A., Leal-Almeraz, T. O., & Thomas, V. M. (2001). Assessing elemental mercury vapor exposure from cultural and religious practices. Environmental Health Perspectives, 109, 779–784. Risher, J.F. (2003). Elemental mercury and inorganic mercury compounds: Human health aspects. World Health Organization & International Programme on Chemical Safety https:// apps. who. int/ iris/ handle/ 10665/ 42607. Robles Carrasco, S.; Díaz-Zorita Bonilla, M.; Fuentes Mateo, V. and García Sanjuán, L. (2017). “Bioarchaeological analysis at the copper age site of Valencina de la Concepción (Seville, Spain): The PP4- Montelirio Sector”. In T. Tomé, M. Díaz-Zorita Bonilla, A. Silva, R. Boaventura and C. Cunha (eds.): Current approaches to collective burials in the Late European Prehistory. Proceedings of the UISPP Conference (Burgos, Spain 2014) Volume 14/Session A25b. Archaeopress. Oxford: 103–118. Rocha, L.; De Oliveira, J.; Barrocas Dias, C.; Mirão, J.; Dias, L. and Manhita, A. (2018). About the presence of exotic materials in some funerary megalithic monuments in Alentejo (Portugal): The cases of cinnabar and amber, In: Cruz, A. and Gibaja Bao, J. F. (eds.): Interchange in Pre- and L.García Sanjuán et al. 1 3 Protohistory. Case Studies in Iberia, Romania, Turkey and Israel. BAR International Series 2891, 83–90. Oxford, Archaeopress. Rodríguez, J., Montero-Ruiz, I., Hunt-Ortiz, M., & García-Pavón, E. (2020). Cinnabar provenance of Chalcolithic red pigments in the Iberian Peninsula: A lead isotope study. Geoarchaeology, 35(6), 871–882. Rodríguez García, A. M., Hernández Sobrino, A., Zarzalejos Prieto, M., Castillo Martos, M., Rodríguez Martín-Doimeadios, R. C., & Herrero Chamorro, M. A. (2021). Un paseo por la historia del mercurio. Almadén y sus minas reconocidas como EuChemS Historical Landmark. Anales de la Química, 117(4), 300–311. Rogerio-Candelera, M. A., Karen Herrera, L., Zélia Miller, A., García Sanjuán, L., Mora Molina, C., Wheatley, D. W., Justo, A., & Saiz-Jiménez, C. (2013). Allochthonous red pigments used in burial practices at the Copper Age site of Valencina de la Concepción (Sevilla, Spain. characterisation and social dimension. Journal of Archaeological Science, 40, 279–290. https:// doi. org/ 10. 1016/j. jas. 2012. 08. 004 Sánchez Meseguer, J., & Galán Saulnier, C. (2004). El ‘Cerro de La Encantada.’ In M. R. García & J. Morales (Eds.), La Península Ibérica en el II Milenio A.C. Poblados y fortificaciones, Cuenca (pp. 115–172) Sancho Mengod, M. (2013). El Cinabrio en Teotihuacan: Color Ritual y Funerario. TFG Universitat de València, Facultat de Geografia i Història, Grau Història de L’art. Santana Falcón, I. (1991): “Excavación arqueológica de emergencia en un dolmen del término municipal de Castilleja de Guzmán (Sevilla)”, Anuario Arqueológico de Andalucía/1989, 446–449. Sevilla, Junta de Andalucía Schotsmans, E. M. J., Busacca, G., Lin, S. C., Vasić, M., Lingle, A. M., Veropoulidou, R., Mazzucato, C., Tibbetts, B., Haddow, S. D., Somel, M., Toksoy-Köksal, F., Knüsel, C. J., & Milella, M. (2022). New insights on commemoration of the dead through mortuary and architectural use of pigments at Neolithic Çatalhöyük, Turkey. Scientifc Reports, Nature, 12, 4055. https:// doi. org/ 10. 1038/ s41598- 022- 07284-3 Sevillano-López. D, González, F.J. (2011). Mining and minerals trade on the Silk Road to the ancient literary sources: 2 BC to 10 AD centuries in J. E. Ortiz, O. Puche, I. Rábano and L. F. Mazadiego (eds.) History of Research in Mineral Resources. Cuadernos del Museo Geominero, 13. Instituto Geológico y Minero de España, Madrid, 43–60. Siddal, R. (2018). Mineral pigments in archaeology: Their analysis and the range of available materials. Minerals, 8, 201. Stanish, C., Earle, T., García Sanjuán, L., Tantaleán, H., & Barrientos, G. (forthcoming). Early monumentality, ritual, and political complexity: Formative Peru and Copper Age Iberia. Current Anthropology Street, R., Kabera, G., & Connolly, C. (2015). Metallic mercury use by South African traditional health practitioners: Perceptions and practices. Environmental Health, 14, 67. https:// doi. org/ 10. 1186/ s12940- 015- 0053-4 Streets, D. G., Devane, M. K., Lu, Z., Bond, T. C., Sunderland, E. M., & Jacob, D. J. (2011). All-time releases of mercury to the atmosphere from human activities. Environmental Science and Technology, 45, 10485–10491. Sugiyama, S & López Luján, L. (2006). Sacrificios de consagración en la Pirámide de la Luna, Teotihuacan. En: Sugiyama, S., López Luján, L. (ed.). Sacrificios de consagración en la Pirámide de la Luna. México D.F.: CONACULTA-INAH (Instituto Nacional de Antropología e Historia), 25–52. Sundseth, K., Pacyna, J. M., Pacyna, E. G., Pirrone, N., & Thorne, R. J. (2017). Global sources and pathways of mercury in the context of human health. International Journal of Environmental Research Public Health, 14, 105. https:// doi. org/ 10. 3390/ ijerp h1401 0105 Torino, M., Boldsen, J., Tarp, P., etal. (2015). Convento di San Francesco a Folloni: The function of a Medieval Franciscan Friary seen through the burials. Herit Sci, 3, 27. https:// doi. org/ 10. 1186/ s40494- 015- 0056-z Valera, A. C. (2020). O Sepulcro 4 dos Perdigões. Um Tholos da Segunda Metade Do 3° Milénio AC. (Vol. 2). Lisboa: Perdigões Monográfica. Vargas Jiménez, J.M. (2021). El Tholos de La Pastora, Un Monumento Megalítico Singular del Yacimiento Prehistórico de Valencina. Arquitectura, Geoarqueología y Contexto, Sevilla, Diputación de Sevilla Vázquez de Agredos, M. L. (2007). Los colores y las técnicas de la pintura mural maya. Anales Del Museo De América, 15, 55–66. 1 3 Beautiful, Magic, Lethal: aSocial Perspective ofCinnabar… Vijande-Vila, E. Díaz-Zorita Bonilla, M. Morell-Rovira, B. Olalde, Í. Sánchez-Barba Muñoz, L.P. Domínguez-Bella, S. Emslie, S.D. Becerra-Martín, S. Rubio-Salvador, Á. Fernández-Sánchez, D.S. Cantillo-Duarte, J.J. Alemán-Aguilera, I. Moreno-Márquez, A. Molina-Piernas, E. Ramírez- Amador, J.L. Gómez-Sánchez, M.L. Botella-López, M.C. Rodríguez-Vidal, J. Ramos-Muñoz, J. (2022). At the beginnings of the funerary Megalithism in Iberia at Campo de Hockey necropolis. Scientific Reports, 12 (1). VVAA. (1996). Navegantes y Náufragos. Galeones en la Ruta del Mercurio. Barcelona, Fundación La Caixa. Walser, J. W., Kristjansdottir, S., Gowland, R., & Desnica, N. (2019). Volcanoes, medicine, and monasticism: Investigating mercury exposure in medieval Iceland. International Journal of Osteoarchaeology, 29(1), 48–61. https:// doi. org/ 10. 1002/ oa. 2712 Weldon, M. M., Smolinski, M. S., Maroufi, A., Hasty, B. W., Gilliss, D. L., & Boulanger, L. L. (2000). Mercury poisoning associated with a Mexican beauty cream. Western Journal of Medicine, 173(1), 15–18. Wendroff, A. (2005). Magico-religious mercury use in Caribbean and Latino communities: Pollution, persistence, and politics. Environmental Practice, 7(2), 87–96. https:// doi. org/ 10. 1017/ S1466 04660 50501 2X Whittle, A. (2018). Times of their lives. Hunting History in the Archaeology of Neolithic Europe, Oxford, Oxbow Books WHO (World Health Organization) (2017a). Riesgos para la salud relacionados con el trabajo y el medioambiente asociados a la extracción de oro artesanal o a pequeña escala. Environmental and Occupational Health Hazards Associated with Artisanal and Small-Scale Gold Mining. Ginebra. https:// apps. who. int/ iris/ handle/ 10665/ 247195. Wittmers, L. E., Jr., Wallgren, J., Alich, A., Aufderheide, A., & Rapp, G. (1988). Lead in bone IV: Distribution of lead in the human skeleton. Archives of Environmental Health, 43(381), 391. WHO (World Health Organization) (2007). Exposure to mercury: a major public health concern. Geneva, (Preventing disease through healthy environments series). WHO (World Health Organization) (2017b). Mercury and health. Key facts. Geneva.(https:// who. int/ newsr oom/ factsheets/ detail/ mercu ry- and- health) Yamada, M., Tohno, S., Tohno, Y., Minami, T., Ichiib, M., & Okazakib, Y. (1995). Accumulation of mercury in excavated bones of two natives in Japan. The Science of the Total Environment, 162, 253–256. Yang, M., Wang, L., Zhang, T., Zhu, A., Sun, Y., Zhao, J., Liu, D., Wang, Q., & Zen, K. (2020). Different proteomic profiles of cinnabar upon therapeutic and toxic exposure reveal distinctive biological manifestations. Journal of Ethnopharmacology, 253, 112668. https:// doi. org/ 10. 1016/j. jep. 2020. 112668 Yusà, V., Pérez, R., Suelves, T., Corpas-Burgos, F., Gormáz, M., Dualde, P., Coscolla, C., Quiles, J., Roca, M., & Vento, M. (2017). Biomonitoring of mercury in hair of breastfeeding mothers living in the Valencian Region (Spain). Levels and Predictors of Exposure. Chemosphere, 187, 106–113. https:// doi. org/ 10. 1016/j. chemo sphere. 2017. 08. 100 Zahir, F., Rizwi, S. J., Haq, S. K., & Khan, R. H. (2005). Low dose mercury toxicity and human health. Environmental Toxicology and Pharmacology, 20, 351–360. https:// doi. org/ 10. 1016/j. etap. 2005. 03. 007 Zapatero Magdaleno, P. (1990). La Velilla, un enterramiento de tradición dolménica en el valle de Valdavia. Boletín De La Institución Tello Téllez De Meneses, 60, 7–13. Zarzalejos Prieto, M., Esteban Borrajo, G., Hevia Gómez, P., & Pina Burón, M. R. (2020b). Celeberrimo Sisaponensi regione in Baetica miniario metallo… Vías de investigación sobre el cinabrio hispano en época romana. Anejos a CuPAUAM, 4, 307–316. https:// doi. org/ 10. 15366/ ane4. ochoa 2020. 02 Zarzalejos Prieto, M., Fernández Ochoa, C., Esteban, G. and Hevia, P. (2012). “El paisaje minero antiguo de la comarca de Almadén (Ciudad Real). Nuevas aportaciones sobre el territorium de Sisapo”. En A. Orejas y Ch. Rico (eds.): Minería y Metalurgia Antiguas. Visiones y Revisiones. Madrid: 129–150. Zarzalejos Prieto, M.; Hevia Gómez, P. and Esteban Borrajo, G. (2020a). Usos y aplicaciones del cinabrio en la península Ibérica entre la Prehistoria reciente y el fin del Mundo Antiguo: una revisión necesaria. In: Zarzalejos Prieto, M.; Hevia Gómez, P. and Mansilla Plaza, L. (eds.): El Oro Rojo en la Antigüedad. Perspectivas de Investigación sobre los Usos y Aplicaciones del Cinabrio entre la Prehistoria y el Fin del Mundo Antiguo, 15–64. Madrid, UNED L.García Sanjuán et al. 1 3 Zarzalejos Prieto, M. (2019). Oro rojo en la vida y en la muerte: El cinabrio como marcador de estatus en el mundo antiguo. El patrimonio geológico y minero. Identidad y motor de desarrollo. Cuadernos del Museo Geominero 29: 43–57 (Mansilla Plaza, L., Mata Perelló, J.M., eds.). Instituto Geológico y Minero de España, Madrid. Zilhão, J. etal. (2010). Symbolic use of marine shells and mineral pigments by Iberian Neandertals. Proceedings of the National Academy of Sciences 107 (3): 1023–1028. http:// www. pnas. org/ content/ early/2010/01/06/0914088107 Zioła-Frankowska, A., Dąbrowski, M., Kubaszewski, L., Rogala, P., Kowalski, A., & Frankowski, M. (2017). An analysis of factors affecting the mercury content in the human femoral bone. Environmental Science and Pollution Research, 24(1), 547–557. https:// doi. org/ 10. 1007/ s11356- 016- 7784-9 Publisher’s Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Authors and Affiliations LeonardoGarcíaSanjuán1 · RaquelMonteroArtús1 · Steven D.Emslie2 · José AntonioLozanoRodríguez3 · MiriamLuciañez‑Triviño1 * Leonardo García Sanjuán [email protected] Raquel Montero Artús raquelmonter[email protected] Steven D. Emslie [email protected] José Antonio Lozano Rodríguez [email protected] Miriam Luciañez-Triviño [email protected] 1 Departamento de Prehistoria y Arqueología, Facultad de Geografía E Historia, Universidad de Sevilla, C/María de Padilla S/N, 41004Seville, Spain 2 Department ofBiology andMarine Biology, University ofNorth Carolina, Wilmington, NC28403, USA 3 Canary Islands Oceanographic Center (COC), Spanish Institute ofOceanography (IEO), Spanish Research Council (CSIC), SantaCruzdeTenerife, Spain