The cyanobacterial ribosomal-associated protein LrtA is involved in post-stress survival in synechocystis sp. PCC 6803

RESEARCH ARTICLE The Cyanobacterial Ribosomal-Associated Protein LrtA Is Involved in Post-Stress Survival in Synechocystis sp. PCC 6803 Carla V. Galmozzi ¤ , Francisco J. Florencio, M. Isabel Muro-Pastor* Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC-Universidad de Sevilla, Sevilla, Spain ¤Current address: Zentrum für Molekulare Biologie der Universität Heidelberg, Heidelberg, Germany *[email protected] Abstract A light-repressed transcript encodes the LrtA protein in cyanobacteria. We show that halflife of lrtA transcript from Synechocystis sp. PCC 6803 is higher in dark-treated cells as compared to light-grown cells, suggesting post-transcriptional control of lrtA expression. The lrtA 5´ untranslated leader region is involved in that darkness-dependent regulation. We also found that Synechocystis sp. PCC 6803 LrtA is a ribosome-associated protein present in both 30S and 70S ribosomal particles. In order to investigate the function of this protein we have constructed a deletion mutant of the lrtA gene. Cells lacking LrtA (ΔlrtA) had significantly lower amount of 70S particles and a greater amount of 30S and 50S particles, suggesting a role of LrtA in stabilizing 70S particles. Synechocystis strains with different amounts of LrtA protein: wild-type, ΔlrtA, and LrtAS (overexpressing lrtA) showed no differences in their growth rate under standard laboratory conditions. However, a clear LrtA dose-dependent effect was observed in the presence of the antibiotic tylosin, being the LrtAS strains the most sensitive. Similar results were obtained under hyperosmotic stress caused by sorbitol. Conversely, after prolonged periods of starvation, ΔlrtA strains were delayed in their growth with respect to the wild-type and the LrtAS strains. A positive role of LrtA protein in post-stress survival is proposed. Introduction Photosynthetic organisms need to harmonize various processes to diurnal changes in light intensity and nutrient availability. Cyanobacteria exhibit a great adaptability to changing environmental conditions through changes in gene expression. Numerous genome expression analyses have been carried out in these organisms, especially in the model Synechocystis sp. PCC 6803 (hereafter Synechocystis). Most of these studies are related to the transcriptional response to different environmental changes [1]. However, far fewer studies address the translational regulation in cyanobacteria. There is ever-increasing evidence that numerous protein factors interact with the ribosome to regulate protein synthesis and modulate the expression profile of the cell in response to different environmental stresses [2]. In this context, this study aims to advance in the knowledge of LrtA protein as a potential modulator of translation. PLOS ONE | DOI:10.1371/journal.pone.0159346 July 21, 2016 1/24 a11111 OPEN ACCESS Citation: Galmozzi CV, Florencio FJ, Muro-Pastor MI (2016) The Cyanobacterial Ribosomal-Associated Protein LrtA Is Involved in Post-Stress Survival in Synechocystis sp. PCC 6803. PLoS ONE 11(7): e0159346. doi:10.1371/journal.pone.0159346 Editor: Franck Chauvat, CEA-Saclay, FRANCE Received: May 5, 2016 Accepted: June 30, 2016 Published: July 21, 2016 Copyright: © 2016 Galmozzi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by Junta de Andalucía (grant P07-CVI-02792 and group BIO-284) and Spanish Ministerio de Economía y Competitividad (MINECO) and Fondo Social Europeo (FSE) (grant BFU2013-41712-P). Competing Interests: The authors have declared that no competing interests exist. The lrtA gene was originally identified in Synechococcus sp. PCC 7002 as a gene encoding a light-repressed transcript [3]. A deeper study of the regulation and stability of this transcript revealed that its half-life was much higher in darkness than in light [4]. The same study also predicted extensive secondary structure for the 5’untranslated region (5’UTR) of lrtA, which is also deduced in Synechocystis lrtA 5’UTR. While lrtA homologous genes are present in most of the cyanobacterial sequenced genomes the function of LrtA remains unknown. LrtA is related to a family of proteins originally named the sigma-54 modulation proteins, based on the observation that the mutation of the corresponding ORF, downstream of the Klebsiella pneumoniae rpoN gene, causes an increase in the expression levels of sigma-54-dependent promoters [5]. Accumulated evidences for several members of this family indicates that these proteins, widespread among bacteria, are ribosome-associated proteins whose function deals with modulation of ribosome activity in order to preserve their integrity and aiding cell survival under stress. Among the most studied members of this family are two Escherichia coli proteins: YfiA (pY, RaiA) and YhbH (HPF: hibernation promotion factor). While YfiA is involved in the inactivation of 70S ribosomes, HPF promotes the formation of translationally inactive 100S ribosome particles. These particles result from 70S ribosome dimerization in stationary-phase [6]. Detailed information is available about how these proteins bind to the ribosome and affect protein synthesis [7]. However much less is known about their role in vivo. Deletion of yfiA or hpf genes had no effect on cell growth or cell viability even in the context of stress situations in which these genes are putatively involved [6,8]. For a broad view of the ribosome regulation in the frame of the bacterial translation stress response, see for example [2,9,10]. Phylogenetic analysis revealed that most bacteria have at least one HPF homologue. These homologues have been classified into three types: long HPF, short HPF and YfiA, based on the presence of a conserved domain and additional homologous sequences [11]. According to this classification, cyanobacterial lrtA gene might encode a long HPF homologue. Formation of 100S ribosomes is mediated by RMF (ribosome modulation factor) and short HPF in Gammaproteobacteria species, similar to E.coli, whereas this process is mediated only by long HPF in other bacterial species. The 100S ribosome lacks translational activity, because RMF binds to the 70S ribosome close to the peptidyl transferase center and peptide exit tunnel, and 100S ribosome do not contain tRNA and mRNA [12]. The cyanobacterial lrtA gene product also displays sequence similarity to a spinach plastidspecific ribosomal protein (PSRP-1) that is present in the chloroplast stroma either unbound or associated to the 30S ribosomal subunit [13–15]. This observation is consistent with the cyanobacterial origin of the chloroplast [16]. More recent data suggest that PSRP-1 is a functional homologue of the E.coli pY protein [17,18]. It stabilizes the ribosome, preventing dissociation, and is recycled by the ribosome-recycling factor (RRF) and translation elongation factor G (EF-G). A sequence alignment of several members of the LrtA protein family is shown in Fig 1. Our current knowledge about lrtA from cyanobacteria is limited. In Synechocystis, transcription of lrtA is mainly dependent on the group 2 sigma factor SigB, because lrtA expression is significantly reduced in a sigB knock-out strain. SigB protein level increases 2-fold after a shift from continuous light to darkness [20–22]. In Synechococcus elongatus PCC 7942 lrtA expression profiles are not dependent on the clock genes (kai) since this gene is not significantly altered in the kaiABC-null strains [23]. Finally, numerous gene expression and proteomic datasets for Synechocystis, under diverse environmental and genetic conditions have been reported in the literature [24]. Although differential accumulation of lrtA gene or LrtA protein has been described [25], the function of LrtA remains unknown in cyanobacteria. In this study we characterize the lrtA gene from Synechocystis. We show that the cyanobacterial LrtA protein is associated to the 30S ribosomal subunit and that a lrtA mutant has a LrtA Protein from Synechocystis PLOS ONE | DOI:10.1371/journal.pone.0159346 July 21, 2016 2/24 LrtA Protein from Synechocystis PLOS ONE | DOI:10.1371/journal.pone.0159346 July 21, 2016 3/24 decreased amount of ribosomal 70S particles. A comparative analysis of lrtA-null (ΔlrtA), lrtA- overexpressing (LrtAS) and wild-type strains leads us to propose a positive role of LrtA in post-stress survival. Materials and Methods Bacterial strains, growth conditions and chlorophyll determination Synechocystis was grown photoautotrophically at 30°C in BG11 medium [26] supplemented with 1 g l -1 of NaHCO 3 (BG11C) and bubbled with a continuous stream of 1% (v/v) CO 2 in air under continuous illumination (50 μmol of photons m -2 s -1 , white light from fluorescent lamps). In cultures with ammonium as nitrogen source 10 mM NH 4 Cl was added to BG11C and the medium was buffered with 20 mM TES (pH 7.5). Dark conditions were obtained by wrapping culture flasks with aluminium foil. For plate cultures, BG11C medium was supplemented with 1% (w/v) agar. Kanamycin was added to a final concentration of 50 μg/ml, spectinomycin and streptomycin were added to a final concentration of 2.5 μg/ml when required. In the case of antibiotic sensitivity assays tylosin was added in a concentration range between 200 and 300 ng/ml, erythromycin between 10 and 40 ng/ml, tetracycline at 2 and 5 μg/ ml, chloramphenicol at 0.25 and 0.5 μg/ml, lincomycin at 5 and 10 ng/ml and puromycin at 5 and 10 μg/ml. Escherichia coli DH5α(Bethesda Research Laboratories) grown in Luria Bertani medium was used for plasmid construction and replication. Cultures were supplemented with 100 μg/ml ampicillin or 50 μg/ml kanamycin when required. Total chlorophyll was measured in methanolic extracts of Synechocystis cells [27]. General DNA manipulations and construction of mutant Synechocystis strains All recombinant DNA experiments were performed according to standard procedures [28]. Southern blotting was done as previously described [28]. To clone the lrtA gene, two primers based on the published sequence of the Synechocystis genome [29] were synthesized: lrtA1 and lrtA2 (S1 Table). A 1865 bp DNA fragment covering the lrtA genomic region (sll0947) was obtained by PCR amplification using these oligonucleotides. This fragment was cloned into pGEM-T to generate pGEM-lrtA. ASynechocystis ΔlrtA mutant strain was obtained by replacing the lrtA gene with a neomycin phosphotransferase (npt) containing cassette (C.K1) [30], which confers kanamycin resistance (Km r ). The inactivating plasmids pGEM-lrtA::C.K1(+) and pGEM-lrtA::CK1(-) were generated by replacing a 655 bp EcoRI-BamHI fragment from pGEM-lrtA by the 1.3 kb HincII C.K1 cassette, cloned in both orientations. Transformation of Synechocystis cells was carried out as previously described [31]. Correct integration of the C.K1 cassette and total segregation of the mutant chromosomes in the ΔlrtA (+) and ΔlrtA (-) mutant strains was confirmed by Southern blot analysis (S1 Fig). Fig 1. Sequence alignment of several LrtA homologous proteins from bacteria and chloroplasts. E. coli, Escherichia coli; Azotobacter, Azotobacter vinelandii; Klebsiella, Klebsiella pneumoniae, Lactobacillus, Lactobacillus delbrueckii; Staphylococcus, Staphylococcus aureus; Deinococcus, Deinococcus radiodurans; Anabaena, Anabaena sp. PCC 7120; Prochl. MIT9313, Prochlorococcus MIT 9313; Synechocystis, Synechocystis sp. PCC 6803, Synechococcus, Synechococcus sp. PCC 7002, Chlamydomonas, Chlamydomonas reinhardtii; Arabidopsis, Arabidopsis thaliana, Spinacea, Spinacia oleracea. In the case of the chloroplastic proteins [Chlamydomonas reinhardtii (XP 001697734.1), Arabidopsis thaliana (NP 568447.1) and Spinacia oleracea (AAA 34039.1)] the chloroplast transit peptide sequence is not shown. Shaded boxes indicate similar amino acids conserved in at least half of the aligned sequences. We considered as similar amino acids the following groups (L,I,V), (K,R), (E,D), (S,T) and (Y,F). The Synechocystis sequence is shown in bold and the residues corresponding to the six amino acids involved in ribosome binding in E.coli YfiA [19] are written in white on black background. doi:10.1371/journal.pone.0159346.g001 LrtA Protein from Synechocystis PLOS ONE | DOI:10.1371/journal.pone.0159346 July 21, 2016 4/24 In order to generate the complemented Synechocystis strain (LrtAC), a wild type lrtA gene was introduced in the ΔlrtA mutant strain by transformation. A 1206 bp DNA fragment including lrtA promoter, 5’-UTR and ORF was amplified by PCR from Synechocystis genomic DNA, using the oligonucleotides lrtAKpnI and lrtASmaI (S1 Table). This fragment was cloned in a plasmid containing a 2 kb region of the non-essential nrsBACD operon, used as a platform for integration of constructs [32]. A streptomycin/spectinomycin resistance cassette from pHP45O[33] was placed downstream of the lrtA locus (S1 Fig). The resulting plasmid was used to transform ΔlrtA Synechocystis cells and the integration of lrtA gene into the nrsBACD operon by homologous recombination was confirmed by Southern blot analysis. In order to overexpress lrtA under control of the P trc promoter, a 591 bp DNA fragment containing lrtA ORF was amplified by PCR from Synechocystis genomic DNA, using the oligonucleotides lrtABspHI and lrtASmaI.2 (S1 Table). This fragment was cloned into pTrc99A NcoI-SmaI digested [34]. An EcoRV-SmaI fragment from the resulting plasmid, containing the P trc promoter, a ribosome binding site from pTrc99A and the lrtA ORF, was cloned in a plasmid harbouring a 2 kb region of the non-essential nrsBACD operon [32]. A streptomycin/spectinomycin resistance cassette from pHP45O[33] was placed downstream of the lrtA ORF. The resulting plasmid was used to transform wild-type or ΔlrtA Synechocystis cells, producing LrtAS_WT and LrtAS_ΔlrtA, respectively. Primer extension analysis Primer extension analysis was carried out using oligonucleotide lrtAR4 (S1 Table) as previously described [35]. RNA isolation and Northern blot hybridization Total RNA was isolated from 25 ml samples of Synechocystis cultures at the early-exponential phase (3–4μg chlorophyll/ml), except for experiments involving different growth phases. RNA extraction was performed by vortexing cells in the presence of phenol:chloroform and acid washed baked glass beads (0.25–0.3 mm diameter; Braun, Melsungen, Germany) as previously described [36]. RNA blotting to nylon membranes (Hybond N-plus; Amersham), prehybridization, hybridization and washes were in accordance with Amersham instruction manuals. A 655 bp EcoRI-BamHI fragment from plasmid pGEM-lrtA was used as lrtA probe for Northern blotting experiments. In order to quantify signals, in all the cases the filters were reprobed with aHindIII-BamHI 580 bp probe from plasmid pAV1100 that contains the constitutively expressed rnpB gene from Synechocystis [37]. To determine cpm of radioactive areas in Northern blot hybridizations either an InstantImager Electronic Autoradiography apparatus (Packard Instrument Company, Meriden, CT) or a Cyclone Phosphor System (Packard) were used. LrtA expression and purification The pGEM-lrtA-his plasmid was created by replacing the stop codon of the lrtA gene of pGEM-lrtA by 6 histidine codons followed by a new stop codon. An NdeI-BamHI fragment from pGEM-lrtA-his plasmid encompassing the lrtA-his gene was cloned into NdeI-BamHI digested pET3a plasmid (Novagen, La Jolla, CA) to generate pET3a-lrtA-his. Exponentially growing E.coli BL21 cells transformed with pET3a-lrtA-his were treated with 0.5 mM of isopropyl ß-D-1-thiogalactopyranoside for 4 h. For purification of LrtA-His 6 cells were collected, resuspended in buffer A (20 mM sodium phosphate, pH 7.4, 0,5 M NaCl) with 1 mM phenylmethylsulfonyl fluoride and disrupted by sonication. The lysate was centrifuged at 18,000 x g for 10 min. LrtA-His 6 from the supernatant was purified by Ni-affinity chromatography using His-Bind resin matrix (Novagen) and following the manufacturer’s instructions. LrtA Protein from Synechocystis PLOS ONE | DOI:10.1371/journal.pone.0159346 July 21, 2016 5/24 Anti-LrtA antibody production and Western Blotting Anti-LrtA antiserum was obtained according to standard immunization protocols by injecting one milligram of purified LrtA-His 6 protein in rabbits. Antibodies against E.coli S12 and L13 proteins were kindly provided by Richard Brimacombe. For Western blot analysis proteins were fractionated on 12% SDS-PAGE according to the method of Laemmli [38] and immunoblotted with anti-LrtA (1:4000), anti-S12 (1:2000) or anti-L13 (1:2000). The ECL Plus immunoblotting system (Amersham) was used to detect the different antigens with anti-rabbit (1:12,000) or anti-sheep (1:5,000) secondary antibodies conjugated to horseradish peroxidase. Preparation of crude extracts from Synechocystis cells To analyse the abundance of LrtA in cells of Synechocystis grown under different conditions, crude extracts were prepared using glass beads as previously described [39] in 50 mM Hepes- NaOH buffer, pH 7.0, 50 mM KCl. Equal volumes (typically 10 μl) of the processed samples were loaded on SDS-PAGE. Isolation of ribosomal particles For ribosome isolation 3 l of Synechocystis cultures were collected by centrifugation at 10.000 x g. Cells were mixed in a mortar with double weight of alumina, broken by grinding and resuspended in buffer A (20 mM Tris-HCl pH 8.0, 20 mM NH 4 Cl, 5 mM ß-mercaptoethanol) supplemented with 1 or 10 mM magnesium acetate, in order to dissociate or not the 30S and 50S ribosomal subunits. The crude extract was centrifuged at 10.000 x gfor 10 min to eliminate alumina and unbroken cells. The supernatant was then centrifuged at 30.000 x gfor 30 min and the supernatant was collected (S30 fraction). 200–300 μl of S30 fraction (approximately 1 mg of protein) was layered onto 10–30% continuous sucrose density gradient in buffer A with the appropriated magnesium acetate concentration and centrifuged at 2°C for 8 h at 25.000 rpm, using a Beckman SW41 Ti rotor. Gradients were analyzed with an ISCO UA-6 detector with continuous monitoring at 254 nm. 0.5 ml fractions were collected and total protein was precipitated with 10% trichloroacetic acid. Results Synechocystis lrtA transcript analysis under light-dark transitions The analysis of the Synechocystis genome showed the existence of an open reading frame (sll0947) product that displayed strong similarity to the previously identified Synechococcus sp. PCC 7002 LrtA protein (Fig 1)[3]. We analyzed the transcript level of the Synechocystis lrtA gene under light-dark transitions and found that lrtA mRNA increased around 7 times 15 minutes after transferring exponentially growing cells (2–3μg chlorophyll/ml of culture) to the dark (Fig 2A). The lrtA mRNA level quickly decreased upon re-illumination of the culture. Transcription of many cyanobacterial genes shows an inverse behaviour upon a light-dark shift, reflecting a decreased metabolism of these photosynthetic organisms under darkness [40]. Therefore, as a control, Fig 2A also shows that under the same conditions the glnA (the gene encoding glutamine synthetase I) mRNA followed an inverse pattern to lrtA. The darkness-dependent up-regulation of the lrtA mRNA was found to be transitory. The maximal level of lrtA mRNA was observed within 15 min of darkness, and thereafter transcript level decreased slowly, reaching level similar to those present under continuous illumination after 3–4h(Fig 2A and S2 Fig). In contrast to the lrtA mRNA, the level of the LrtA protein did not LrtA Protein from Synechocystis PLOS ONE | DOI:10.1371/journal.pone.0159346 July 21, 2016 6/24 change in response to light-dark-light switches as evidenced by Western blotting experiments using an anti-LrtA polyclonal serum (Fig 2B). The effect of darkness on the lrtA transcript stability was further investigated. To that end, transcription was blocked by addition of rifampicin to Synechocystis cells in the light or in the dark. Samples were taken at different times and transcript levels were determined by Northern blotting. In the light, the lrtA transcript level decreased with a half-life of 3.2 ± 0.1 min. However, the lrtA transcript from dark-treated cells showed a longer half-life (5.3 ± 0.25 min) (Fig 3). These data suggest that a post-transcriptional mechanism is responsible for the darknessdependent regulation of the lrtA gene. Expression of the lrtA gene is controlled by the growth phase E.coli proteins related to LrtA, YfiA and YhbH, have been shown to be up-regulated during stationary phase [41]. In order to investigate whether Synechocystis lrtA is controlled in a similar way, the level of lrtA transcript was determined by Northern blot at different time points along the growth curve. As shown in Fig 4A, the level of the lrtA transcript was maximal in the early exponential growth phase, thereafter decreasing progressively as the cell density Fig 2. Darkness-dependent regulation of lrtA mRNA and protein levels. A, Total RNA was isolated from early-log-phase Synechocystis cells growing under normal illumination conditions (Light), after being subjected to darkness for 15, 30 or 60 min, and after re-illumination of the culture for 15 or 30 min. 15 μgof total RNA was loaded per lane. Levels of lrtA and glnA mRNA were determined by Northern blotting. All the filters were stripped and re-hybridized with a rnpB gene probe as loading control. The data shown are representative of four independent Northern-blot experiments showing similar results. B, Level of LrtA protein was determined by Western blotting using anti-LrtA serum. 6 μg of total protein was loaded per lane. The data shown are representative of three independent Western-blot experiments showing similar results. doi:10.1371/journal.pone.0159346.g002 LrtA Protein from Synechocystis PLOS ONE | DOI:10.1371/journal.pone.0159346 July 21, 2016 7/24 increased. The level of LrtA protein was also lower when cultures were reaching stationary phase (Fig 4B). Stationary growth phase is reached by a deficiency in one or more macronutrients. We investigated whether nitrogen starvation also promotes a decrease in the level of lrtA mRNA. For that, exponentially growing Synechocystis cells using ammonium as nitrogen source were transferred to a nitrogen-free medium. Fig 4C shows that lrtA expression is strongly down regulated by nitrogen deficiency. The lrtA 5´ untranslated leader region is involved in darkness-dependent regulation Determination of the Synechocystis lrtA transcription start point by RNA primer extension analysis demonstrated the presence of a long untranslated leader region in the lrtA mRNA. Thus, only one transcription start site was found 312 bp upstream of the lrtA ATG start codon (S3 Fig). This result agrees with the previously described transcription initiation for the lrtA gene by both primer extension [22] and transcriptomic [42]. To characterize the function of the lrtA gene, a strain lacking this gene was generated by replacing the complete open reading frame by a kanamycin resistance cassette (see M&M for details). Complete segregation of the mutation and absence of lrtA transcript were confirmed Fig 3. Dark-increased stability of lrtA transcript. Rifampicin (400 μg/ml) was added to light (Light) growing Synechocystis cells or to 15 min dark-treated cells (Dark). Samples were taken, under the same conditions, at the indicated times and total RNA was isolated. 15 μg of total RNA was loaded per lane. Levels of lrtA mRNA were determined by Northern blotting. Radioactive signals intensity were normalized with respect to the rnpB RNA level, and plotted against time. Values are the average of two independent experiments. doi:10.1371/journal.pone.0159346.g003 LrtA Protein from Synechocystis PLOS ONE | DOI:10.1371/journal.pone.0159346 July 21, 2016 8/24 by Southern blotting and Northern blotting, respectively. The absence of the LrtA protein was also confirmed by Western blotting experiments (S1 Fig). The ΔlrtA strain was viable, indicating that lrtA is a dispensable gene in Synechocystis. To investigate the position of putative cis-regulatory elements responsible for the darknessdependent regulation of the lrtA gene we generated a construct were the lrtA ORF (without the leader region) was fused to the P trc promoter, which was reported to act as a constitutive promoter in Synechocystis [43]. This lrtA version was introduced in the dispensable nrsBACD Fig 4. lrtA mRNA and protein levels as a function of cell growth. A, Aliquots of Synechocystis cells growing in BG11C medium were taken at different cell densities. Chlorophyll was measured in methanolic extracts (open circles). Total RNA was isolated from each sample (numbers in the plot), 15 μg of total RNA was loaded per lane and levels of lrtA mRNA were determined by Northern blotting. Radiactive signals were quantified, values were normalized with respect to the rnpB RNA level (mRNA relative) and plotted (closed squares). Data are expressed in percentage of the maximum value obtained in the exponential growth phase. The data shown are representative of three independent Northern-blot experiments showing similar results. B, LrtA level is affected by the growth phase. Total protein was isolated from exponential-growth-phase Synechocystis cells (2.2 μg Chl/ml of culture) or late linear-growth-phase cells (>20 μg Chl/ml of culture). 5 μgof total protein was loaded per lane. Level of LrtA protein was determined by Western blotting using anti-LrtA serum. As a control for protein loading, membranes were also incubated with anti-GSI. Glutamine synthetase I (GSI) is constitutively expressed, independently of the growth phase in Synechocystis cells. The data shown are representative of three independent Western-blot experiments showing similar results. C, Early exponentially growing Synechocystis cells that used ammonium as nitrogen source (time 0) were centrifuged, washed and resuspended in BG11C medium lacking any nitrogen source (BG11 0 C). Aliquots of culture were taken at the indicated time for total RNA isolation and levels of lrtA mRNA were determined by Northern blotting. 15 μg of total RNA was loaded per lane. The data shown are representative of three independent Northern-blot experiments showing similar results. doi:10.1371/journal.pone.0159346.g004 LrtA Protein from Synechocystis PLOS ONE | DOI:10.1371/journal.pone.0159346 July 21, 2016 9/24 in S4 Fig, the ribosome-binding site may be possibly hindered by the secondary structure, which may represent an additional point of control of the lrtA expression. In a classification of cyanobacterial promoters that is based on sigma factors that recognize them, lrtA promoter is assigned to type 2 having only the -10 hexamer. Promoters from some nitrogen-regulated genes belong to the same type and contain a motif for the nitrogen control regulator NtcA [20,21]. We show here that lrtA expression is strongly down regulated under nitrogen starvation. Interestingly a putative NtcA binding site centred at position -26.5 upstream of the lrtA TSP was found (S7 Fig). This localization of NtcA binding site is characteristic of NtcA-repressed genes [47]. According to our results, NtcA repression of the lrtA promoter could be hypothesized. All together these results indicate that the Synechocystis lrtA transcript accumulation integrates several environmental signals. Fig 9. Effect of sorbitol and NaCl on the growth of Synechocystis strains with different LrtA amounts. Growth curves of the indicated strains under standard conditions in BG11C medium (A), in BG11C supplemented with 0.7 M NaCl (B) or in BG11C with 0.5 M sorbitol (C). ΔlrtA(+) and ΔlrtA(-) refer to the orientation of the antibiotic resistance cassette in the same or opposite direction respect to the lrtA gene, respectively. Each growth curve represents the mean of three independent experiments showing similar results. D, LrtA level is affected by the presence of sorbitol. Total protein was isolated from exponentially growing Synechocystis cells incubated with 0.8 M sorbitol for up to twenty-four hours. 5 μg of total protein was loaded per lane. Level of LrtA protein was determined, at different times after sorbitol addition, by Western blotting using anti-LrtA serum. As a control for protein loading, membranes were incubated also with anti-GSI. The data shown are representative of three independent Western-blot experiments showing similar results. doi:10.1371/journal.pone.0159346.g009 LrtA Protein from Synechocystis PLOS ONE | DOI:10.1371/journal.pone.0159346 July 21, 2016 16 / 24 Fig 10. Growth of Synechocystis strains with different amounts of LrtA, in refreshing conditions after stationary phase. A, Cells of the wild-type and mutant strains were cultivated photoautotrophically (50 μmol photons m -2 s -1 , 1% (v/v) CO 2 in air) during three weeks. These cells were used to inoculate fresh medium at 0.1 OD 730 (day 1). Growth was followed for 5 days and at that time aliquots of the cultures were photographed. The data represent average values of three independent experiments. B, Growth after darkness treatment. At day “0”exponentially growing cells under standard illumination conditions (light) were transferred to darkness during four days and then re-illuminated. Arrows indicate the day on which the cultures were photographed. ΔlrtA(+) and ΔlrtA(-) refer to the orientation of the antibiotic resistance cassette in the same or opposite direction respect to the lrtA gene, respectively. doi:10.1371/journal.pone.0159346.g010 LrtA Protein from Synechocystis PLOS ONE | DOI:10.1371/journal.pone.0159346 July 21, 2016 17 / 24 In agreement with the role of LrtA orthologs in other bacteria, association of LrtA protein with ribosomes has been demonstrated in this work. In fact, the six basic amino acids involved in binding between the homologous protein YfiA and the ribosome [19], are conserved in LrtA (Fig 1). However, part of the LrtA protein could be detected in a protein fraction that is not associated with ribosomes. This result indicates that, in agreement with the observations for other members of this protein family, LrtA is a ribosome-associated factor, not a canonical ribosomal protein. Concerning the possible role of LrtA, the results obtained with the Synechocystis ΔlrtA strain clearly indicate that LrtA stabilizes ribosomal 70S particles under normal growth conditions at all stages of growth. The comparison of ribosomal profiles from the wild type and ΔlrtA cells also suggests a drop in the number of ribosomes in the last strain. Nevertheless, with the available data we cannot distinguish between a possible defect in the translation initiation or early blockage of elongation. However no differences could be observed in the growth curves of ΔlrtA and WT strains under laboratory conditions. Drug sensitivity assays carried out with tylosin and erythromycin showed a higher growth of the ΔlrtA strain versus the wild type in the presence of these antibiotics. Ribosome binding sites of tylosin and erythromycin have been located in approximately the same region of the large ribosomal subunit, within a hydrophobic crevice of the peptide exit tunnel. These antibiotics inhibit protein synthesis by blocking the egress of nascent polypeptides [48,49]. Members of the LrtA protein family (YfiA, HPF) have ribosome binding sites that overlap with those of the mRNA, transfer RNA and initiation factors [7]. This localization in the intersubunit entrance-exit channel is relatively close to the macrolides binding site, which may explain why the presence or absence of LrtA modifies the effect of tylosin and erythromycin in Synechocystis cells. One of the best-characterized proteins of the LrtA family is the E.coli YfiA [50]. In vitro studies demonstrated that this protein inhibits translation and is present in ribosomes of cells that reached stationary phase or are subjected to a temperature downshift [51]. Structural work established that YfiA binds to the 30S subunit and probably stabilizes a state of this subunit with high affinity for the 50S subunit, thereby preventing subunit dissociation. The role attributed to this protein was to modulate ribosome activity as a function of cell stress and transiently repress protein synthesis, specifically during cold adaptation [19]. The other E.coli protein of the family, YhbH or HPF, is also related to the down-regulation of protein synthesis, upon entry into stationary phase [7]. In contrast, LrtA protein from Synechocystis is present in ribosomes isolated from exponentially growing cells and moreover it is down-regulated in stationary phase or nitrogen-starved cells. Thus, LrtA function does not seem to be related to the translation during periods of limited growth. Rather, the results presented here indicate a role of LrtA in post-stress survival, specifically when growth restarts after long starvation periods. As mentioned in the introduction, LrtA belongs to the long HPF type. Proteins similar in length, sequence and domain structure are present in other bacterial groups [12]. Similar to the case of Synechocystis, these bacterial species have no orthologues of E.coli rmf and hpf. Although in vivo studies of these proteins are limited, a detailed analysis of Lactococcus lactis YfiA, a long HPF type protein, has been recently published [52]. This study demonstrates that deletion of the yfiA gene has no effect on the growth rate but diminishes the survival of Lactococcus lactis under energy-starving conditions. Parallel to what we observed with LrtA in cyanobacteria, the growth of Lactococcus lactis ΔyfiA was reduced compared to wild type upon longer starvation and the lag times before re-growth increased more than that of wild type after prolonged starvation. These results suggest that the in vivo effect of lacking such proteins is quite similar. Based on our results, we cannot consider that LrtA is a stress protein; rather our working hypothesis is that LrtA must have a function under normal growth conditions, but may not be LrtA Protein from Synechocystis PLOS ONE | DOI:10.1371/journal.pone.0159346 July 21, 2016 18 / 24 easily observable as a difference in the mutant growth curve under laboratory conditions. In fact it has been described that long type HPF proteins from other bacteria are continually expressed throughout all growth phases [12]. To explain the positive effect of LrtA in restoring growth after stress, a role in re-association of ribosomal subunits can be hypothesized in view of our observation on the amount of 70S ribosomal particles in WT and mutant strains (Fig 7). Localization of Synechocystis LrtA in the ribosome must be the same that has been described for other proteins of the family (pY or PSRP-1) namely, overlapping mRNA and tRNAs positions [18,19]. Consistently with studies of these proteins [8,11], LrtA could be released from the 70S ribosome by translation factors such as RRF and EF-G, leading to subunit dissociation and translation initiation mediated by IF3 and other initiation factors. We speculate that LrtA- mediated stabilization of 70S ribosomes may have a function in the physiology of Synechocsytis and not just as a “storing”factor involved in sequestering a fraction of the ribosomes as idle 70S monomers, when a lower number of translating ribosomes is required by the cell, as have been proposed for pY protein. The results we have obtained in experiments with sorbitol are intriguing (Fig 9), since strains lacking LrtA are able to grow much faster than the wild-type in the presence of 0.5 M sorbitol. It has been reported that incubation of Synechocystis cells in medium supplemented with 0.5 M sorbitol decreases the cytoplasmic volume to 30% of the original value within 10 min and then remained at this level [44]. Consistent with our hypothesis about LrtA function, it is possible that the absence of this protein favours the normal association/dissociation cycle of ribosomal subunits in the translation process with the hyperosmotic pressure provoked by sorbitol. This would explain the growth results obtained with strains with different LrtA protein level, as well as the down-regulation in LrtA abundance observed in wild-type cells after sorbitol treatment. It has been described that heat shock induces ribosome subunit dissociation and translating ribosome can dissociate erroneously [2]. It is interesting the observation that lrtA transcript abundance is temperature-dependent in Synechocystis (S8 Fig). The transcript is up-regulated at high temperature, probably because of the increase of SigB sigma factor in these conditions [20]. This result also supports a role of LrtA on the stabilization of the 70S particles. Although several aspects of the LrtA function are not yet known, this work is, to our knowledge, the first study that addresses the role of this family of proteins in cyanobacteria. Supporting Information S1 Fig. Scheme of modified loci in the constructed strains. A, Schematic representation of the lrtA genomic region in the WT and in the ΔlrtA mutant strains. ΔlrtA(+) and ΔlrtA(-) refer to the orientation of the antibiotic resistance cassette in the same or opposite direction respect to the lrtA gene, respectively. B, Southern blot analysis of Synechocystis WT and ΔlrtA mutant. Genomic DNA was digested with HincII and HaeII, and hybridized using the DNA fragment indicated in panel Aas a probe. C, Northern blot analysis of Synechocystis WT and the ΔlrtA mutant. Total RNA was isolated from 15 min dark-incubated Synechocystis cells and lrtA transcript was detected by Northern blotting. Then the filter was stripped and rehybridized with a rnpB probe as loading control. D,Synechocystis WT and ΔlrtA mutant total extract proteins were separated by SDS-PAGE and subjected to Western blotting using anti-LrtA antibodies. E, Schematic representation of the nrsBACD locus in the Synechocystis LrtAC strain. F, Schematic representation of the nrsBACD locus in the Synechocystis LrtAS strains. (TIF) S2 Fig. Darkness-dependent regulation of lrtA mRNA. Total RNA was isolated from earlylog-phase Synechocystis cells growing under normal illumination conditions (Light, time 0) or LrtA Protein from Synechocystis PLOS ONE | DOI:10.1371/journal.pone.0159346 July 21, 2016 19 / 24 after being subjected to darkness for 15, 30, 60, 240 or 480 min. 7 μg of total RNA was loaded per lane. Level of lrtA mRNA was determined by Northern blotting. The filter was stripped and re-hybridized with a rnpB gene probe as loading control. The values represented in the histogram are relative to that of cells after 15 min in darkness (100%). (TIF) S3 Fig. Primer extension analysis. Oligonucleotide used for primer extension analysis of the lrtA transcript was lrtAR4 (S1 Table), complementary to positions—76 to -95 relative to the translation start of lrtA. Primer extension assays were carried out with RNA isolated from cells growing under normal illumination conditions (Light) or subjected to darkness for 15 min. End-labelled 20-pb DNA ladder (Bio-Rad) was used as a marker. (TIF) S4 Fig. lrtA leader region. Predicted secondary structure o the 5' UTR of lrtA (from -312 to +10 with respect to translational start) according to Mfold [53]. The transcriptional start [42], Shine-Dalgarno region (SD) and translational start (boxed) are indicated. (TIF) S5 Fig. Effect of different antibiotics on the growth of Synechocystis wild type and ΔlrtA mutant. Ten-fold serial dilutions of each Synechocystis culture were spotted on BG11C medium plates (control) or in BG11C supplemented with the indicated concentration of antibiotic and photographed after 10 days of growth. ΔlrtA(+) refer to the orientation of the antibiotic resistance cassette in the same direction respect to the lrtA gene. (TIF) S6 Fig. Growth of Synechocystis strains with different amounts of LrtA, in refreshing conditions after nitrogen starvation. Early-log-phase Synechocystis cells of the wild-type and mutant strains growing photoautotrophically (50 μmol photons m -2 s -1 , 1% (v/v) CO 2 in air) were centrifuged, resuspended in BG11 0 C medium (lacking any nitrogen source) and incubated under normal illumination conditions for one week. These cells were used to inoculate fresh BG11C medium at 0.1 OD 730 (day 1). Growth was followed for 5 days. The data represent average values of three independent experiments. ΔlrtA(+) and ΔlrtA(-) refer to the orientation of the antibiotic resistance cassette in the same or opposite direction respect to the lrtA gene, respectively. (TIF) S7 Fig. Alignment of different NtcA-repressed promoter sequences. NtcA binding sites are chaded in gray, -10 regions are boxed and the transcriptional start is underlined. The consensus sequence of the NtcA-activated promoter (centered at -41.5) is also shown. gifA 7120 (gifA gene from Anabaena sp. PCC 7120 [54]), gifA 6803 (gifA gene from Synechocystis sp. PCC 6803 [47]), gifB 6803 (gifB gene from Synechocystis sp. PCC 6803 [47]), rbcL 7120 (rbcL gene from Anabaena sp. PCC 7120 [55]). (TIF) S8 Fig. Temperature-dependent regulation of lrtA mRNA. Early-log-phase Synechocystis cells growing under normal conditions at 30°C (time 0) were transferred to different temperatures and total RNA was isolated at the indicated times. 7 μg of total RNA was loaded per lane. Level of lrtA mRNA was determined by Northern blotting. The filter was stripped and rehybridized with a rnpB gene probe as loading control. The values represented in the histogram are relative to that of control cells after 15 min at 30°C (100%). (TIF) LrtA Protein from Synechocystis PLOS ONE | DOI:10.1371/journal.pone.0159346 July 21, 2016 20 / 24 S1 Table. Oligonucleotides used in this study. 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