Multienzymatic processes involving baeyer–villiger monooxygenases

catalysts Review Multienzymatic Processes Involving Baeyer–Villiger Monooxygenases Gonzalo de Gonzalo 1,* and Andrés R. Alcántara 2,*   Citation: de Gonzalo, G.; Alcántara, A.R. Multienzymatic Processes Involving Baeyer–Villiger Monooxygenases. Catalysts 2021,11, 605. https://doi.org/10.3390/ catal11050605 Academic Editor: Richard Daniellou Received: 20 April 2021 Accepted: 6 May 2021 Published: 8 May 2021 Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). 1Organic Chemistry Department, University of Sevilla, c/Profesor García González 2, 41012 Sevilla, Spain 2 Unit of Organic and Pharmaceutical Chemistry, Department of Chemistry in Pharmaceutical Sciences, Faculty of Pharmacy, Complutense University of Madrid, Plaza de Ramón y Cajal, s/n, 28040 Madrid, Spain *Correspondence: [email protected] (G.d.G.); [email protected] (A.R.A.); Tel.: +34-95-455-99-97 (G.d.G.); +34-91-394-18-21 (A.R.A.) Abstract: Baeyer–Villiger monooxygenases (BVMOs) are flavin-dependent oxidative enzymes capable of catalyzing the insertion of an oxygen atom between a carbonylic Csp 2 and the Csp 3 at the alpha position, therefore transforming linear and cyclic ketones into esters and lactones. These enzymes are dependent on nicotinamides (NAD(P)H) for the flavin reduction and subsequent reaction with molecular oxygen. BVMOs can be included in cascade reactions, coupled to other redox enzymes, such as alcohol dehydrogenases (ADHs) or ene-reductases (EREDs), so that the direct conversion of alcohols or α , β -unsaturated carbonylic compounds to the corresponding esters can be achieved. In the present review, the different synthetic methodologies that have been performed by employing multienzymatic strategies with BVMOs combining whole cells or isolated enzymes, through sequential or parallel methods, are described, with the aim of highlighting the advantages of performing multienzymatic systems, and show the recent advances for overcoming the drawbacks of using BVMOs in these techniques. Keywords: biocatalysis; whole cells; cascade reactions; redox enzymes; monooxygenases; Baeyer– Villiger alcohol dehydrogenases; ene-reductases 1. Introduction The elucidation and rational understanding of the internal organization of the different biocatalytic reactions occurring inside biological cells, in which several enzymatic reactions proceed in a concatenated manner, is one of the basis of Systems Biology [ 1 ]. The development of this discipline has fostered the design of coupled systems of biocatalytic reactions, and the number of publications dealing with this topic has increased considerably during the last years [ 2 – 11 ]. In this area, oxidoreductases are enzymes frequently used in cascade reactions, as usually a pair of these enzymes are simultaneously applied for the required in situ recycling of the cofactors [ 12 – 15 ]. Inside this type of enzymes, Baeyer–Villiger monooxygenases (BVMOs) are undoubtedly one of the most attractive members of this family; these flavin-dependent oxidative enzymes [ 16 – 20 ] are capable of catalyzing the insertion of an oxygen atom between a carbonylic Csp 2 and the Csp 3 at the alpha position, therefore transforming linear and cyclic ketones into esters and lactones [20–27], as schematized in Scheme 1. BVMOs require nicotinamides (NADPH) for the flavin reduction to FADH 2 , which reacts with molecular oxygen to form the reactive peroxyflavin responsible for the substrate oxidation. As NADPH is an expensive compound and its presence at high concentrations can inhibit the biocatalyst, it has to be recycled to obtain feasible biocatalytic procedures. In the last years, more than 100 BVMOs have been cloned and overexpressed. In many cases, the natural role of those BVMOs is not identified, while in others they seem to take part in the synthesis of secondary metabolites. Most of the BVMOs, the so-called type I BVMOs, can be included in the B subclass of flavin dependent monooxygenases [ 28 ], Catalysts 2021,11, 605. https://doi.org/10.3390/catal11050605 https://www.mdpi.com/journal/catalysts Catalysts 2021,11, 605 2 of 25 according to their protein sequence motifs, electron donor and type of oxygenation reaction. Thus, they are single-component enzymes possessing two α / β Rossmann-like domains for the FAD and NADPH binding, respectively, and they keep this last one bound during catalysis, while the substrate binds after the flavin-peroxide is formed [ 17 ]. BVMO catalysis started by NADPH binding and subsequent flavin reduction, after which the NADPH cofactor adopts a stable position. A stable peroxyflavin is the catalytically active specie, formed by the reaction of the reduced flavin with molecular oxygen [ 29 ]. Type II BVMOs are part of the class C of flavin dependent monooxygenases, which are two-component monooxygenases [ 30 ], but this type of BVMOs has been scarcely employed in biocatalysis due the requirement of these two components. Catalysts 2021, 11, x FOR PEER REVIEW 2 of 25 Scheme 1. BVMOs-catalyzed oxidation of ketones to furnish esters. BVMOs require nicotinamides (NADPH) for the flavin reduction to FADH2, which reacts with molecular oxygen to form the reactive peroxyflavin responsible for the substrate oxidation. As NADPH is an expensive compound and its presence at high concentrations can inhibit the biocatalyst, it has to be recycled to obtain feasible biocatalytic procedures. In the last years, more than 100 BVMOs have been cloned and overexpressed. In many cases, the natural role of those BVMOs is not identified, while in others they seem to take part in the synthesis of secondary metabolites. Most of the BVMOs, the so-called type I BVMOs, can be included in the B subclass of flavin dependent monooxygenases [28], according to their protein sequence motifs, electron donor and type of oxygenation reaction. Thus, they are single-component enzymes possessing two α/β Rossmann-like domains for the FAD and NADPH binding, respectively, and they keep this last one bound during catalysis, while the substrate binds after the flavin-peroxide is formed [17]. BVMO catalysis started by NADPH binding and subsequent flavin reduction, after which the NADPH cofactor adopts a stable position. A stable peroxyflavin is the catalytically active specie, formed by the reaction of the reduced flavin with molecular oxygen [29]. Type II BVMOs are part of the class C of flavin dependent monooxygenases, which are two-component monooxygenases [30], but this type of BVMOs has been scarcely employed in biocatalysis due the requirement of these two components. Most of the BVMOs are present on prokaryotes and some unicellular eukaryotic organisms such as filamentous fungi. Several bacterial BVMOs have been applied with biocatalytic purposes; even some fungal BVMOs have also been discovered and characterized in the last few years [31]. Both storage and operational stability of these biocatalysts is interesting for their biotechnological applications. For most of the BVMOs, which presents a certain stability, lyophilization in presence of different additives is a method of interest because it simplifies the enzyme transport and storage [32]. Normally, these biocatalysts perform their activity at mild reaction conditions (aqueous media at neutral pH and room temperature). In the last few years several examples of thermostable BVMOs (wild type and mutants), able to catalyze reactions at high temperatures, have been reported [33,34], with the aim of increasing the applicability of these valuable enzymes [35,36]. Baeyer–Villiger monooxygenases were first discovered in the 1960s, but they have not been widely applied with biocatalytic purposes for the preparation of high valuable compounds as esters, lactones and sulfoxides, among others. Advances in genome mining have allowed scientists to discover several BVMOs active on different types of compounds, but still nowadays, cyclohexanone monooxygenase (CHMO) from Acinetobacter calcoaceticus NCIMB 9871, discovered more than 40 years ago [37], is the BVMO with the highest applicability due to its large substrate profile and excellent selectivity. Nevertheless, CHMO presents some drawbacks as its thermal instability and its low stability to organic co-solvents [38]. Remarkably, most BVMOs display an excellent behavior when employed in multi-step cascade reactions coupled to other enzymes. In most of these reactions, lactones are obtained as final products. These cyclic esters of carboxylic acids, containing a 1-oxacycloalkan-2-one structure, are a class of secondary metabolites, thus presenting a wide range of biological activities as anti-inflammatory, antimicrobial or anticancer compounds [39]. Lactones can be also employed in cosmetics and perfume industry, in polymer chemistry, as agrochemicals or in food industry as flavoring agents. By Scheme 1. BVMOs-catalyzed oxidation of ketones to furnish esters. Most of the BVMOs are present on prokaryotes and some unicellular eukaryotic organisms such as filamentous fungi. Several bacterial BVMOs have been applied with biocatalytic purposes; even some fungal BVMOs have also been discovered and characterized in the last few years [ 31 ]. Both storage and operational stability of these biocatalysts is interesting for their biotechnological applications. For most of the BVMOs, which presents a certain stability, lyophilization in presence of different additives is a method of interest because it simplifies the enzyme transport and storage [ 32 ]. Normally, these biocatalysts perform their activity at mild reaction conditions (aqueous media at neutral pH and room temperature). In the last few years several examples of thermostable BVMOs (wild type and mutants), able to catalyze reactions at high temperatures, have been reported [ 33 , 34 ], with the aim of increasing the applicability of these valuable enzymes [35,36]. Baeyer–Villiger monooxygenases were first discovered in the 1960s, but they have not been widely applied with biocatalytic purposes for the preparation of high valuable compounds as esters, lactones and sulfoxides, among others. Advances in genome mining have allowed scientists to discover several BVMOs active on different types of compounds, but still nowadays, cyclohexanone monooxygenase (CHMO) from Acinetobacter calcoaceticus NCIMB 9871, discovered more than 40 years ago [ 37 ], is the BVMO with the highest applicability due to its large substrate profile and excellent selectivity. Nevertheless, CHMO presents some drawbacks as its thermal instability and its low stability to organic co-solvents [ 38 ]. Remarkably, most BVMOs display an excellent behavior when employed in multi-step cascade reactions coupled to other enzymes. In most of these reactions, lactones are obtained as final products. These cyclic esters of carboxylic acids, containing a 1-oxacycloalkan-2-one structure, are a class of secondary metabolites, thus presenting a wide range of biological activities as anti-inflammatory, antimicrobial or anticancer compounds [ 39 ]. Lactones can be also employed in cosmetics and perfume industry, in polymer chemistry, as agrochemicals or in food industry as flavoring agents. By this reason, several methodologies have been performed for their preparation. The use of biocatalysts for the synthesis of lactones presents some advantages regarding the classical methods, as only molecular oxygen is required as oxidant while working under mild (pH and temperature) reaction conditions, being achieved in general high regio- and/or enantioselectivites. The combination of two or more biocatalysts also allowed avoiding the isolation and purification of the reaction intermediates, thus increasing the atom economy of the processes. However, the use of BVMOs in these synthetic procedures can present Catalysts 2021,11, 605 3 of 25 some limitations, as low enzyme expression and stability, the NADPH-dependence, and substrate and product inhibition. In this review, we comment on different reported cases in which enzymatic cascades, including BVMOs, have been applied for synthesizing valuable esters and lactones, aiming at stressing the applicability of this approach and promoting an increased employ of these excellent biocatalysts. For practical reasons, we have classified the reported examples into two main categories, cascades including BVMOs catalyzed by whole cells or by isolated enzymes. 2. Multi-Step Reactions Including BVMO Activity Catalyzed by Whole Cells Generally speaking, the use of whole cells makes the cascade easier compared to those employing isolated biocatalysts, as the recycling of the required cofactors is produced inside the cell metabolic machinery, so that it is not mandatory to implement an external recycling methodology. The most attractive methodology using whole cells implies the use of àla carte engineered cells, inside which different enzymatic activities are overexpressed in order to generate the desired multi-step procedure [ 40 ]. This methodology, which has been sometimes termed as Systems Biocatalysis [ 41 ], presents several advantages [40,42–45] : (i) as already mentioned, the intra-cellular medium provides the natural enzymatic environment and the cofactors regeneration machinery; (ii) it is relatively easy and economical to have available cells by cultivation without any additional downstream process; (iii) enzymes inside cell walls and membranes are somehow protected from extreme reaction conditions, and (iv) the different enzymes involved in the cascade are co-localized inside cells, so that their local concentration is increased, therefore reducing the diffusion of intermediates. 2.1. Multi-Step Reactions Including Alcohol Dehydrogenases/BVMO Activity Catalyzed by Whole Cells Most of the examples of biocatalytic cascades employing BVMOs have been developed together with alcohol dehydrogenases (ADHs, EC 1.1.1.x), also called ketoreductases (KREDs). These enzymes are able to catalyze the reversible reduction of carbonyl compounds into the corresponding alcohols (Scheme 2), in general with high selectivity [ 46 – 49 ]. ADHs require the presence of nicotinamide cofactors for performing their activity, being required to employ effective regeneration systems if these enzymes are employed as isolated biocatalysts. Catalysts 2021, 11, x FOR PEER REVIEW 3 of 25 this reason, several methodologies have been performed for their preparation. The use of biocatalysts for the synthesis of lactones presents some advantages regarding the classical methods, as only molecular oxygen is required as oxidant while working under mild (pH and temperature) reaction conditions, being achieved in general high regio- and/or enantioselectivites. The combination of two or more biocatalysts also allowed avoiding the isolation and purification of the reaction intermediates, thus increasing the atom economy of the processes. However, the use of BVMOs in these synthetic procedures can present some limitations, as low enzyme expression and stability, the NADPH-dependence, and substrate and product inhibition. In this review, we comment on different reported cases in which enzymatic cascades, including BVMOs, have been applied for synthesizing valuable esters and lactones, aiming at stressing the applicability of this approach and promoting an increased employ of these excellent biocatalysts. For practical reasons, we have classified the reported examples into two main categories, cascades including BVMOs catalyzed by whole cells or by isolated enzymes. 2. Multi-Step Reactions Including BVMO Activity Catalyzed by Whole Cells Generally speaking, the use of whole cells makes the cascade easier compared to those employing isolated biocatalysts, as the recycling of the required cofactors is produced inside the cell metabolic machinery, so that it is not mandatory to implement an external recycling methodology. The most attractive methodology using whole cells implies the use of à la carte engineered cells, inside which different enzymatic activities are overexpressed in order to generate the desired multi-step procedure [40]. This methodology, which has been sometimes termed as Systems Biocatalysis [41], presents several advantages [40,42–45]: (i) as already mentioned, the intra-cellular medium provides the natural enzymatic environment and the cofactors regeneration machinery; (ii) it is relatively easy and economical to have available cells by cultivation without any additional downstream process; (iii) enzymes inside cell walls and membranes are somehow protected from extreme reaction conditions, and (iv) the different enzymes involved in the cascade are co-localized inside cells, so that their local concentration is increased, therefore reducing the diffusion of intermediates. 2.1. Multi-Step Reactions Including Alcohol Dehydrogenases/BVMO Activity Catalyzed by Whole Cells Most of the examples of biocatalytic cascades employing BVMOs have been developed together with alcohol dehydrogenases (ADHs, EC 1.1.1.x), also called ketoreductases (KREDs). These enzymes are able to catalyze the reversible reduction of carbonyl compounds into the corresponding alcohols (Scheme 2), in general with high selectivity [46– 49]. ADHs require the presence of nicotinamide cofactors for performing their activity, being required to employ effective regeneration systems if these enzymes are employed as isolated biocatalysts. Scheme 2. Alcohol dehydrogenases catalyzed reversible reduction of carbonyl compounds and oxidation of alcohols. Scheme 2. Alcohol dehydrogenases catalyzed reversible reduction of carbonyl compounds and oxidation of alcohols. Initial studies on linear cascades combining ADHs and BVMOs were performed at the beginning of the 1990s, using whole cells systems for the direct bio-oxidation of alcohols to the corresponding lactones. Thus, the starting alcohol was oxidized to the corresponding ketones by the ADH at the expense of NAD(P) + , whereas the obtained ketone was further oxidized in presence of the BVMO to the desired lactone, employing NAD(P)H which was converted again into NAD(P) + . A pioneer example is the oxidation of alcohol 1 , shown in Scheme 3, employing fractured cells of Acinetobacter calcoacetitus NCIMB to the corresponding lactone 3 , described in 1991 [ 50 ]. At short reaction times, 1 was converted into ketone 2 in a great extent (80% after 6 h). Longer reaction time led to an increase in the Catalysts 2021,11, 605 4 of 25 lactone production, reaching a maximum value of 40% after 48 h, whereas concentration of 2 started to decrease after 10 h. A similar pattern was observed in the bio-oxidation of both endo- and exo-bicyclo [2.2.1]heptan-2-ols. Alcohol concentration decreased as the ketone became formed and lactone concentration started to increase during the course of the biocatalytic process. Fractured cells of A. calcoaceticus were also employed in the bio-oxidation of 6-endo-bicyclo [3.2.0]hept-2-en-6-ol 4 to obtain regioisomeric lactones 5 and 6 with complete conversion after 1 h, as shown in Scheme 3. A 15% of a by-product, which authors assumed that was exo-4, was observed in the crude mixture. Catalysts 2021, 11, x FOR PEER REVIEW 4 of 25 Initial studies on linear cascades combining ADHs and BVMOs were performed at the beginning of the 1990s, using whole cells systems for the direct bio-oxidation of alcohols to the corresponding lactones. Thus, the starting alcohol was oxidized to the corresponding ketones by the ADH at the expense of NAD(P)+, whereas the obtained ketone was further oxidized in presence of the BVMO to the desired lactone, employing NAD(P)H which was converted again into NAD(P)+. A pioneer example is the oxidation of alcohol 1, shown in Scheme 3, employing fractured cells of Acinetobacter calcoacetitus NCIMB to the corresponding lactone 3, described in 1991 [50]. At short reaction times, 1 was converted into ketone 2 in a great extent (80% after 6 h). Longer reaction time led to an increase in the lactone production, reaching a maximum value of 40% after 48 h, whereas concentration of 2 started to decrease after 10 h. A similar pattern was observed in the bio-oxidation of both endo- and exo-bicyclo [2.2.1]heptan-2-ols. Alcohol concentration decreased as the ketone became formed and lactone concentration started to increase during the course of the biocatalytic process. Fractured cells of A. calcoaceticus were also employed in the bio-oxidation of 6-endo-bicyclo [3.2.0]hept-2-en-6-ol 4 to obtain regioisomeric lactones 5 and 6 with complete conversion after 1 h, as shown in Scheme 3. A 15% of a by-product, which authors assumed that was exo-4, was observed in the crude mixture. Scheme 3. Acinetobacter calcoaceticus cells catalyzed biotransformations of alcohols into lactones. The combination of BVMOs and ADHs has been further developed for the preparation of ε-caprolactone (ECL, 9, Scheme 4). This compound is a valuable material with several applications in the field of colorants, adhesives and coating materials, as ECL can be easily polymerized yielding biodegradable thermoplastics and elastomeric polymers [51,52]. By these reasons, several approaches have been studied for its preparation under sustainable reaction conditions. BVMOs and ADHs have been employed well as whole cells or well as isolated enzymes for the synthesis of ECL starting from cyclohexanol 7, in a process in which this alcohol is oxidized by the ADH to cyclohexanone 8, which is converted into ECL in presence of the BVMO, with an in situ NAD(P)H cofactor regeneration. When employing this approach, the stability of the BVMO is critical for the process, as this biocatalyst is very sensitive to the cyclohexanol and ECL concentration in the reaction medium. For this reason, several attempts have been made in order to overcome this bottleneck. Scheme 4. Preparation of ECL 9 and its oligomers through a biocatalytic procedure employing whole cells of Lactobacillus brevis ADH and Acinetobacter calcoaceticus CHMO. Scheme 3. Acinetobacter calcoaceticus cells catalyzed biotransformations of alcohols into lactones. The combination of BVMOs and ADHs has been further developed for the preparation of ε -caprolactone (ECL, 9 , Scheme 4). This compound is a valuable material with several applications in the field of colorants, adhesives and coating materials, as ECL can be easily polymerized yielding biodegradable thermoplastics and elastomeric polymers [ 51 , 52 ]. By these reasons, several approaches have been studied for its preparation under sustainable reaction conditions. BVMOs and ADHs have been employed well as whole cells or well as isolated enzymes for the synthesis of ECL starting from cyclohexanol 7 , in a process in which this alcohol is oxidized by the ADH to cyclohexanone 8 , which is converted into ECL in presence of the BVMO, with an in situ NAD(P)H cofactor regeneration. When employing this approach, the stability of the BVMO is critical for the process, as this biocatalyst is very sensitive to the cyclohexanol and ECL concentration in the reaction medium. For this reason, several attempts have been made in order to overcome this bottleneck. Catalysts 2021, 11, x FOR PEER REVIEW 4 of 25 Initial studies on linear cascades combining ADHs and BVMOs were performed at the beginning of the 1990s, using whole cells systems for the direct bio-oxidation of alcohols to the corresponding lactones. Thus, the starting alcohol was oxidized to the corresponding ketones by the ADH at the expense of NAD(P)+, whereas the obtained ketone was further oxidized in presence of the BVMO to the desired lactone, employing NAD(P)H which was converted again into NAD(P)+. A pioneer example is the oxidation of alcohol 1, shown in Scheme 3, employing fractured cells of Acinetobacter calcoacetitus NCIMB to the corresponding lactone 3, described in 1991 [50]. At short reaction times, 1 was converted into ketone 2 in a great extent (80% after 6 h). Longer reaction time led to an increase in the lactone production, reaching a maximum value of 40% after 48 h, whereas concentration of 2 started to decrease after 10 h. A similar pattern was observed in the bio-oxidation of both endo- and exo-bicyclo [2.2.1]heptan-2-ols. Alcohol concentration decreased as the ketone became formed and lactone concentration started to increase during the course of the biocatalytic process. Fractured cells of A. calcoaceticus were also employed in the bio-oxidation of 6-endo-bicyclo [3.2.0]hept-2-en-6-ol 4 to obtain regioisomeric lactones 5 and 6 with complete conversion after 1 h, as shown in Scheme 3. A 15% of a by-product, which authors assumed that was exo-4, was observed in the crude mixture. Scheme 3. Acinetobacter calcoaceticus cells catalyzed biotransformations of alcohols into lactones. The combination of BVMOs and ADHs has been further developed for the preparation of ε-caprolactone (ECL, 9, Scheme 4). This compound is a valuable material with several applications in the field of colorants, adhesives and coating materials, as ECL can be easily polymerized yielding biodegradable thermoplastics and elastomeric polymers [51,52]. By these reasons, several approaches have been studied for its preparation under sustainable reaction conditions. BVMOs and ADHs have been employed well as whole cells or well as isolated enzymes for the synthesis of ECL starting from cyclohexanol 7, in a process in which this alcohol is oxidized by the ADH to cyclohexanone 8, which is converted into ECL in presence of the BVMO, with an in situ NAD(P)H cofactor regeneration. When employing this approach, the stability of the BVMO is critical for the process, as this biocatalyst is very sensitive to the cyclohexanol and ECL concentration in the reaction medium. For this reason, several attempts have been made in order to overcome this bottleneck. Scheme 4. Preparation of ECL 9 and its oligomers through a biocatalytic procedure employing whole cells of Lactobacillus brevis ADH and Acinetobacter calcoaceticus CHMO. Scheme 4. Preparation of ECL 9 and its oligomers through a biocatalytic procedure employing whole cells of Lactobacillus brevis ADH and Acinetobacter calcoaceticus CHMO. Thus, the bienzymatic system employing whole cells of ADH-BVMO for the preparation of ECL can be coupled with the lipase-catalyzed hydrolysis of this compound in order to obtain oligo-caprolactone. This approach was performed for the first time in 2015 [ 53 ]. In order to overcome the BVMO deactivation caused by the presence of ECL in the reaction medium, this product subjected to in situ ring-opening oligomerization catalyzed by CALA (lipase from Candida antarctica, Scheme 4). The formed oligo-ECLs were easily removed from the reaction by extraction or precipitation. CALA is able to catalyze the formation of the polymers even in presence of high amounts of water, not showing any hydrolytic activity on ECL at high substrate concentrations (1.0 M). After a few hours in presence of CALA, the lactone was converted into oligomers with a maximum molecular weight of Catalysts 2021,11, 605 5 of 25 1200 g/mol, which can be transformed into high-molecular weight polymer. Recombinant E. coli cells of Lactobacillus brevis ADH (LbADH) and the stable mutant C376L/M400I of Acinetobacter calcoaceticus cyclohexanone monooxygenase (AcCHMO), at 100 g wet cells weight/mL concentration, were combined with lipase CALA (10 mg/mL lyophilized) in a one-pot process at different concentrations of cyclohexanol. A decrease in the ECL concentration in these reactions was detected when compared to those in absence of the lipase, thus indicating the beneficiary effect of the conversion of the ECL into oligo-ECL. When both the ADH and the BVMO were employed as separate cells, results were better than expressing both biocatalysts into the same cell-system. LbADH/AcCHMO ratio was optimized 1:10 in order to achieve the highest conversion. Addition of acetone and glucose as co-substrates was also positive for a faster regeneration of the endogenous NADPH in the cell systems. The optimized cascade was performed at preparative scale and, after 48 h, a complete conversion was achieved when starting from 200 mM of 7 , yielding a 75% of oligo-ECL and a 25% of ECL. Higher substrate concentrations, 300 mM and 500 mM, afforded lower conversions, 74% and 43% respectively. In a further development of this trienzymatic system, the same authors described the preparation of methyl-substituted ECL derivatives in a one-pot two-step process [ 54 ], as shown in Scheme 5. Catalysts 2021, 11, x FOR PEER REVIEW 5 of 25 Thus, the bienzymatic system employing whole cells of ADH-BVMO for the preparation of ECL can be coupled with the lipase-catalyzed hydrolysis of this compound in order to obtain oligo-caprolactone. This approach was performed for the first time in 2015 [53]. In order to overcome the BVMO deactivation caused by the presence of ECL in the reaction medium, this product subjected to in situ ring-opening oligomerization catalyzed by CALA (lipase from Candida antarctica, Scheme 4). The formed oligo-ECLs were easily removed from the reaction by extraction or precipitation. CALA is able to catalyze the formation of the polymers even in presence of high amounts of water, not showing any hydrolytic activity on ECL at high substrate concentrations (1.0 M). After a few hours in presence of CALA, the lactone was converted into oligomers with a maximum molecular weight of 1200 g/mol, which can be transformed into high-molecular weight polymer. Recombinant E. coli cells of Lactobacillus brevis ADH (LbADH) and the stable mutant C376L/M400I of Acinetobacter calcoaceticus cyclohexanone monooxygenase (AcCHMO), at 100 g wet cells weight/mL concentration, were combined with lipase CALA (10 mg/mL lyophilized) in a one-pot process at different concentrations of cyclohexanol. A decrease in the ECL concentration in these reactions was detected when compared to those in absence of the lipase, thus indicating the beneficiary effect of the conversion of the ECL into oligo-ECL. When both the ADH and the BVMO were employed as separate cells, results were better than expressing both biocatalysts into the same cell-system. LbADH/ AcCHMO ratio was optimized 1:10 in order to achieve the highest conversion. Addition of acetone and glucose as co-substrates was also positive for a faster regeneration of the endogenous NADPH in the cell systems. The optimized cascade was performed at preparative scale and, after 48 h, a complete conversion was achieved when starting from 200 mM of 7, yielding a 75% of oligo-ECL and a 25% of ECL. Higher substrate concentrations, 300 mM and 500 mM, afforded lower conversions, 74% and 43% respectively. In a further development of this trienzymatic system, the same authors described the preparation of methyl-substituted ECL derivatives in a one-pot two-step process [54], as shown in Scheme 5. Scheme 5. Preparation of (S)-4-methylcaprolactone (S)-12 and its oligomers through a biocatalytic procedure employing whole cells of Lactobacillus kefir ADH and Acinetobacter calcoaceticus CHMO mutant QM. A diastereomeric mixture of 4-methylcyclohexanol (10) was employed as starting substrate; under those conditions, AcCHMO was able to convert 4-methylcyclohexanone 11 into the enantiopure lactone (S)-12 with high yield and complete selectivity. Initial experiments were performed with E. coli cells expressing Lactobacillus kefir ADH (LkADH) and wild type AcCHMO in 1:10 ratio (100 g wet cells weight/mL), but the conversions at all concentrations tested (5–20 mM) were low, achieving only a 34% of the enantiopure lactone as highest value when working at 5 mM. Poor conversion values were caused by the low conversion in the ADH catalyzed oxidation, due to the inhibition of this biocatalyst by the ketone 11 formed. In order to improve this process, a faster second oxidation process, with the aim of the fast removing of 11 from the reaction medium was envisioned. Thus, a more stable mutant of AcCHMO was employed (C376L/M400I/T415C/A463C, the so-called AcCHMO QM) and the ratio of the E. coli cells for both biocatalysts was optimized to 1:1. The supply of pure oxygen to the reaction also resulted in a higher conversion, close to 90% after 24 h at 25 °C. The addition of CALA (10 mg/mL) to the enzymatic Scheme 5. Preparation of (S)-4-methylcaprolactone (S)- 12 and its oligomers through a biocatalytic procedure employing whole cells of Lactobacillus kefir ADH and Acinetobacter calcoaceticus CHMO mutant QM. A diastereomeric mixture of 4-methylcyclohexanol ( 10 ) was employed as starting substrate; under those conditions, AcCHMO was able to convert 4-methylcyclohexanone 11 into the enantiopure lactone (S)- 12 with high yield and complete selectivity. Initial experiments were performed with E. coli cells expressing Lactobacillus kefir ADH (LkADH) and wild type AcCHMO in 1:10 ratio (100 g wet cells weight/mL), but the conversions at all concentrations tested (5–20 mM) were low, achieving only a 34% of the enantiopure lactone as highest value when working at 5 mM. Poor conversion values were caused by the low conversion in the ADH catalyzed oxidation, due to the inhibition of this biocatalyst by the ketone 11 formed. In order to improve this process, a faster second oxidation process, with the aim of the fast removing of 11 from the reaction medium was envisioned. Thus, a more stable mutant of AcCHMO was employed (C376L/M400I/T415C/A463C, the so-called AcCHMO QM) and the ratio of the E. coli cells for both biocatalysts was optimized to 1:1. The supply of pure oxygen to the reaction also resulted in a higher conversion, close to 90% after 24 h at 25 ◦ C. The addition of CALA (10 mg/mL) to the enzymatic system led to a further decrease in (S)- 12 concentration, as it was being hydrolyzed into the oligo-(S)-lactone. The production of ECL starting from cyclohexanol employing E. coli cells of ADH and AcCHMO has been studied by using a computational approach in 2017 [ 55 ], applying a kinetic model for the study this cascade reaction in both batch and fed-batch synthesis. To this purpose, a fed-batch synthesis was developed in order to circumvent the CHMO inhibition caused by cyclohexanol, which hampered the use of this substrate at concentrations higher than 60 mM when employed in batch. Thus, 7 was added to the reaction medium at different ratios, from 14.2 to 19.0 µ mol/min; feed rates higher than 17.5 µ mol/min led to the accumulation of 7, therefore reducing the rate formation of ECL due to CHMO inhibition. The amount of ECL in the reaction medium was reduced by hydrolyzing this compound to Catalysts 2021,11, 605 6 of 25 6-hydroxyhexanoic acid ( 13 , Scheme 6) in a process catalyzed by Candida antarctica lipase B (CALB). After 6 h, 162 mM of 13 was obtained with a feed rate of 19 µ mol/min. The addition of CALB maintained a low ECL concentration in the reaction medium (lower than 10 mM). Catalysts 2021, 11, x FOR PEER REVIEW 6 of 25 system led to a further decrease in (S)-12 concentration, as it was being hydrolyzed into the oligo-(S)-lactone. The production of ECL starting from cyclohexanol employing E. coli cells of ADH and AcCHMO has been studied by using a computational approach in 2017 [55], applying a kinetic model for the study this cascade reaction in both batch and fed-batch synthesis. To this purpose, a fed-batch synthesis was developed in order to circumvent the CHMO inhibition caused by cyclohexanol, which hampered the use of this substrate at concentrations higher than 60 mM when employed in batch. Thus, 7 was added to the reaction medium at different ratios, from 14.2 to 19.0 μmol/min; feed rates higher than 17.5 μmol/min led to the accumulation of 7, therefore reducing the rate formation of ECL due to CHMO inhibition. The amount of ECL in the reaction medium was reduced by hydrolyzing this compound to 6-hydroxyhexanoic acid (13, Scheme 6) in a process catalyzed by Candida antarctica lipase B (CALB). After 6 h, 162 mM of 13 was obtained with a feed rate of 19 μmol/min. The addition of CALB maintained a low ECL concentration in the reaction medium (lower than 10 mM). Scheme 6. Preparation of ECL 9 and its oligomers through a biocatalytic procedure employing whole cells of Lactobacillus brevis ADH and Acinetobacter calcoaceticus CHMO M15. The production of ECL has been optimized by performing the co-expression of the BVMO and the ADH in E. coli, employing a DuetTM vector [56], in order to obtain a higher efficiency for the enzymatic cascade. The pRSFDuet plasmid form Novagen was selected as dual expression vector, cloning the AcCHMO QM gene into the first multiple cloning site and the LkADH gene into the second multiple cloning site. With the aim of balancing the expression levels of the cascade enzymes, the ADH gene was subjected to engineering of the ribosome binding site (RBS), employing the native RBS sequence as well as two point mutations. In order to compare the results, both types of enzymes were expressed separately into E. coli cells, performing the biotransformations with an optimized ratio of CHMO: ADH cells 5:1 (100 g wet cells weight/mL) and in presence of glucose and acetone and co-substrates, as higher conversions were obtained than in absence of these compounds. This result can be explained by the difficult diffusion of the NADPH formed in the E. coli cells of the LkADH to the AcCHMO QM cells. After 16 h, it was possible to achieve a 90% conversion from 20 mM of cyclohexanol employing wild type RBS, while much lower conversions were measured with the other two RBS mutations, indicating that a decreased LkADH expression is required to obtain a similar enzyme ratio for optimal conversion. When the same process was carried out with the E. coli cells containing the double gene, a complete conversion was obtained after 16 h for all the three RBS preparations in the absence of co-substrates. In presence of glucose and acetone, complete conversion was also observed for the CHMO co-expressed with one of the RBS mutations of the ADH after 16 h, whereas after 2 h, a 54% conversion was reached. This system was the most efficient for the co-expression of both biocatalysts, suggesting that the ratios of expressed AcCHMO QM and LkADH were similar in this system. When higher substrate concentrations were employed, lower conversion values were achieved, mainly debt to the inhibitory effect of both 7 and 9 on AcCHMO. Very recently, the conversion of cyclohexanol to ECL by combining LkADH with two stable mutants of CHMO has been studied in order to obtain a suitable process at 200 mM scale. Different parameters that affect the process were analyzed and optimized in order to achieve the most productive process for the preparation of the final lactone [57]. The Scheme 6. Preparation of ECL 9 and its oligomers through a biocatalytic procedure employing whole cells of Lactobacillus brevis ADH and Acinetobacter calcoaceticus CHMO M15. The production of ECL has been optimized by performing the co-expression of the BVMO and the ADH in E. coli, employing a Duet TM vector [ 56 ], in order to obtain a higher efficiency for the enzymatic cascade. The pRSFDuet plasmid form Novagen was selected as dual expression vector, cloning the AcCHMO QM gene into the first multiple cloning site and the LkADH gene into the second multiple cloning site. With the aim of balancing the expression levels of the cascade enzymes, the ADH gene was subjected to engineering of the ribosome binding site (RBS), employing the native RBS sequence as well as two point mutations. In order to compare the results, both types of enzymes were expressed separately into E. coli cells, performing the biotransformations with an optimized ratio of CHMO: ADH cells 5:1 (100 g wet cells weight/mL) and in presence of glucose and acetone and co-substrates, as higher conversions were obtained than in absence of these compounds. This result can be explained by the difficult diffusion of the NADPH formed in the E. coli cells of the LkADH to the AcCHMO QM cells. After 16 h, it was possible to achieve a 90% conversion from 20 mM of cyclohexanol employing wild type RBS, while much lower conversions were measured with the other two RBS mutations, indicating that a decreased LkADH expression is required to obtain a similar enzyme ratio for optimal conversion. When the same process was carried out with the E. coli cells containing the double gene, a complete conversion was obtained after 16 h for all the three RBS preparations in the absence of co-substrates. In presence of glucose and acetone, complete conversion was also observed for the CHMO co-expressed with one of the RBS mutations of the ADH after 16 h, whereas after 2 h, a 54% conversion was reached. This system was the most efficient for the co-expression of both biocatalysts, suggesting that the ratios of expressed AcCHMO QM and LkADH were similar in this system. When higher substrate concentrations were employed, lower conversion values were achieved, mainly debt to the inhibitory effect of both 7and 9on AcCHMO. Very recently, the conversion of cyclohexanol to ECL by combining LkADH with two stable mutants of CHMO has been studied in order to obtain a suitable process at 200 mM scale. Different parameters that affect the process were analyzed and optimized in order to achieve the most productive process for the preparation of the final lactone [ 57 ]. The reaction was carried out in a stirred-tank reactor with maintained temperature and pH, bubbling a mixture of oxygen with synthetic air and pumping cyclohexanol at a constant rate proportional to the whole cell cascade activity. As both 7 and 8 present a high vapor pressure, they can be stripped out of the system by the off gas. The key component of the complete system, as previously stated, is CHMO, due to its low stability and the requirement of a proper oxygen supply. This last parameter was optimized by performing a bubble aeration technique. BVMO stability was improved by assessing two CHMO mutants; thus, AcCHMO QM showed a 40% higher long-term stability, whereas AcCHMO M15, containing eight mutations, has a higher oxidative stability combined with an improved thermostability. These two variants were compared employing E. coli cells co-expressing ADH and the CHMO at pH 7.5 and 30 ◦ C, with 200 mM cyclohexanol fed at Catalysts 2021,11, 605 7 of 25 4.5 mM/h. When using AcCHMO QM, the formation of ECL achieved a value of 45 mM after 13 h, but at longer reaction times this concentration decreased to 39 mM, probably debt to the ECL autohydrolysis to yield 13 . The same experiment was performed with AcCHMO M15, reaching a maximum concentration of ECL after 18 h (79 mM). Longer reactions times resulted in substrate accumulation in the reactor with no further formation of the desired product. AcCHMO M15 showed longer activity, but it was not possible to achieve complete conversion. The stability of both CHMO mutants under the process conditions was analyzed; when employing AcCHMO QM, the biocatalyst retained its complete activity for one hour and it decreased to 85% after 8 h. After this reaction time, there was an important drop in the biocatalyst activity. On the other hand, AcCHMO M15 maintained its activity (98% of the initial one) even after 18 h. Thus, when working with AcCHMO QM, fresh cells were added after 8 h, whereas no extra addition was required for AcCHMO M15. Further experiments showed that an increase in the cells loading in the reactor led to a decrease in the volumetric mass transfer, thus resulting in a lower efficiency. The complete optimization of the biocatalytic process allowed scientists to achieve conversions higher than 98% for both CHMO mutants, being possible to obtain a higher final product concentration (21.1 g/L), space time yield (1.1 g/L h) and isolated ECL amount (9.1 g) employing AcCHMO M15, due to its higher operational stability. These values represent a 100% increase regarding the non-optimized processes, indicating the development of a more efficient process for the preparation of ECL. Fusion proteins have been initially employed to enhance the soluble expression of proteins or to improve the enzyme purification, but in the last years some examples have appeared in which these systems have been used with synthetic purposes [ 58 ]. In 2015, the preparation of an enzyme fusion formed by the ADH from Micrococcus luteus NCTC2665 and the BVMO from Pseudomonas putida KT2440 (3.0 g dry cells/L), able to catalyze the double oxidation of long-chain unsaturated secondary alcohols ( 14a – c ) to the corresponding esters ( 16a – c ) through the corresponding ketones 15a – c , was reported [ 59 ], as shown in Scheme 7. These products can be hydrolyzed into valuable ω -hydroxycarboxylic acids and n-alkanoic acids, valuable compounds. The design of the fusion enzyme showed the highest expression level when using a glycine-rich linker, formed by 12 aminoacids, between the two biocatalysts. When the bio-oxidations were carried out with the fused biocatalyst expressed in E. coli, higher conversions were achieved for all the substrates when compared with the independent ADH and BVMO expressed in E. coli. Thus, esters 16a – c can be recovered with conversions higher than 75%, with activities higher than 22 µ mol/g dry cells min. This positive effect of the fusion enzyme in the cascade activity can be debt to a higher functional expression of the BVMO and/or to a better mass transport efficiency, due to the presence of both active centers at close positions. Catalysts 2021,11, 605 8 of 25 Catalysts 2021, 11, x FOR PEER REVIEW 8 of 25 Scheme 7. Preparation of ECL 9 and its oligomers through a biocatalytic procedure employing whole cells of Lactobacillus brevis ADH and Acinetobacter calcoaceticus CHMO M15. 2.2. Multi-Step Reactions Including ERED/BVMO Activity Catalyzed by Whole Cells Ene-reductases (EREDs) from the old yellow enzyme (OYE) family are flavin-de- pendent enzymes that catalyze the chemo- and stereo-selective asymmetric reduction of electronically activated carbon–carbon double bonds [25,27,60–62], as depicted in Scheme 8. Scheme 8. Ene-reductases for the reduction of C=C. Several examples can be found in literature illustrating the use of whole cells-cata- lyzed cascades coupling BVMO and ERED activities. For instance, Silva et al. [63] reported the preparation of ECL 9 starting from either cyclohexenone 17, cyclohexanone 8 or cyclohexanol 7, using whole cells of Brazilian Geotrichum candidum CCT 1205 (Scheme 9). Scheme 9. Preparation of ε-caprolactone 9, using whole cells of Geotrichum candidum CCT 1205. Scheme 7. Preparation of ECL 9 and its oligomers through a biocatalytic procedure employing whole cells of Lactobacillus brevis ADH and Acinetobacter calcoaceticus CHMO M15. 2.2. Multi-Step Reactions Including ERED/BVMO Activity Catalyzed by Whole Cells Ene-reductases (EREDs) from the old yellow enzyme (OYE) family are flavin-dependent enzymes that catalyze the chemo- and stereo-selective asymmetric reduction of electronically activated carbon–carbon double bonds [25,27,60–62], as depicted in Scheme 8. Catalysts 2021, 11, x FOR PEER REVIEW 8 of 25 Scheme 7. Preparation of ECL 9 and its oligomers through a biocatalytic procedure employing whole cells of Lactobacillus brevis ADH and Acinetobacter calcoaceticus CHMO M15. 2.2. Multi-Step Reactions Including ERED/BVMO Activity Catalyzed by Whole Cells Ene-reductases (EREDs) from the old yellow enzyme (OYE) family are flavin-de- pendent enzymes that catalyze the chemo- and stereo-selective asymmetric reduction of electronically activated carbon–carbon double bonds [25,27,60–62], as depicted in Scheme 8. Scheme 8. Ene-reductases for the reduction of C=C. Several examples can be found in literature illustrating the use of whole cells-cata- lyzed cascades coupling BVMO and ERED activities. For instance, Silva et al. [63] reported the preparation of ECL 9 starting from either cyclohexenone 17, cyclohexanone 8 or cyclohexanol 7, using whole cells of Brazilian Geotrichum candidum CCT 1205 (Scheme 9). Scheme 9. Preparation of ε-caprolactone 9, using whole cells of Geotrichum candidum CCT 1205. Scheme 8. Ene-reductases for the reduction of C=C. Several examples can be found in literature illustrating the use of whole cells-catalyzed cascades coupling BVMO and ERED activities. For instance, Silva et al. [ 63 ] reported the preparation of ECL 9starting from either cyclohexenone 17, cyclohexanone 8or cyclohexanol 7, using whole cells of Brazilian Geotrichum candidum CCT 1205 (Scheme 9). Catalysts 2021, 11, x FOR PEER REVIEW 8 of 25 Scheme 7. Preparation of ECL 9 and its oligomers through a biocatalytic procedure employing whole cells of Lactobacillus brevis ADH and Acinetobacter calcoaceticus CHMO M15. 2.2. Multi-Step Reactions Including ERED/BVMO Activity Catalyzed by Whole Cells Ene-reductases (EREDs) from the old yellow enzyme (OYE) family are flavin-de- pendent enzymes that catalyze the chemo- and stereo-selective asymmetric reduction of electronically activated carbon–carbon double bonds [25,27,60–62], as depicted in Scheme 8. Scheme 8. Ene-reductases for the reduction of C=C. Several examples can be found in literature illustrating the use of whole cells-cata- lyzed cascades coupling BVMO and ERED activities. For instance, Silva et al. [63] reported the preparation of ECL 9 starting from either cyclohexenone 17, cyclohexanone 8 or cyclohexanol 7, using whole cells of Brazilian Geotrichum candidum CCT 1205 (Scheme 9). Scheme 9. Preparation of ε-caprolactone 9, using whole cells of Geotrichum candidum CCT 1205. Scheme 9. Preparation of ε-caprolactone 9, using whole cells of Geotrichum candidum CCT 1205. Catalysts 2021,11, 605 9 of 25 When using cyclohexanone 8 as substrate, these authors reported quantitative yields of the desired ε -caprolactone 9 after only 5 h, using a higher amount (3.0 g versus 1.0 g) of whole cells (otherwise, the cascade accumulated 7 and 8 ). Using 7 or 8 as substrates, the reaction was even faster (4 h) than using only 10 g of cells. These results are better than those previously reported by Mihovilovic et al. [ 64 ], leading to 52% of 9 in 48 h, using BVMO expressed in E. coli. Recently, Silva et al. [ 65 ] have shown that it is possible to immobilize the whole cells from Geotrichum candidum CCT 1205 in modified silica (n SiO 2 –Cl, SiO 2 –NH 2 and SiO 2 –SH supports), without altering their catalytic performance and also allowing the stabilization and reuse of the cells. Although the previous example illustrates the use of non-engineered cells, it is becoming more usual the use of genetically modified cells. Hence, Liu and Li described an enantioselective reduction − oxidation − hydrolysis cascade catalyzed by engineered whole cells for the synthesis of (R)-2-alkyl- δ -lactones 22 – 23 (useful flavor and fragrance materials) starting from the corresponding 2-alkylidenecyclopentanones 18–19 (Scheme 10) [66]. Catalysts 2021, 11, x FOR PEER REVIEW 9 of 25 When using cyclohexanone 8 as substrate, these authors reported quantitative yields of the desired ε-caprolactone 9 after only 5 h, using a higher amount (3.0 g versus 1.0 g) of whole cells (otherwise, the cascade accumulated 7 and 8). Using 7 or 8 as substrates, the reaction was even faster (4 h) than using only 10 g of cells. These results are better than those previously reported by Mihovilovic et al. [64], leading to 52% of 9 in 48 h, using BVMO expressed in E.coli. Recently, Silva et al. [65] have shown that it is possible to immobilize the whole cells from Geotrichum candidum CCT 1205 in modified silica (n SiO2– Cl, SiO2–NH2 and SiO2–SH supports), without altering their catalytic performance and also allowing the stabilization and reuse of the cells. Although the previous example illustrates the use of non-engineered cells, it is becoming more usual the use of genetically modified cells. Hence, Liu and Li described an enantioselective reduction−oxidation−hydrolysis cascade catalyzed by engineered whole cells for the synthesis of (R)-2-alkyl-δ-lactones 22–23 (useful flavor and fragrance materials) starting from the corresponding 2-alkylidenecyclopentanones 18−19 (Scheme 10) [66]. Scheme 10. Preparation of enantiopure (R)-2-alkyl-δ-lactones by using a whole-cell-catalyzed cascade involving BVMO activity. In this example, the cascade catalysis was started with 50 mL of cell suspension of Acinetobacter sp. RS1 in Tris buffer (cell density 12 g cdw/L) containing 40 mg of alkylidene ketones 18 or 19 and 20 mg/mL glucose. After 3 h of reaction to allow the ERED-mediated accumulation of alkyl ketones 20 and 21 (R-configuration), 150 mL of Tris buffer containing engineered cells of E. coli (CHMO−GDH) (10 g cdw/L) was added to start the Baeyer– Villiger oxidation. After simultaneous oxidation and hydrolysis for 1.5 h, extraction with ethyl acetate and purification by flash chromatography, compounds (R)-22 (56% yield, 98% ee) or (R)-23 (41% yield, 97% ee) could be obtained. The presence of a hydrolytic activity inside Acinetobacter sp. RS1 cells was responsible for the hydrolysis of the intermediate (S)-22 or (S)-23 leading to δ-hydroxyacids 24 or 25, obtained with low enantioselectivity (E = 8–11). The different enantiomers of carvolactones (7-methyl-4-(prop-1-en-2-yl)oxepan-2- ones) are gaining importance as monomers for the preparation of polymeric thermoplastic elastomers (shape-memory polymers) and pressure-sensitive adhesive components [67– 69]. These carvolactones can be obtained starting from biogenic carvones after a C=C reduction and a Baeyer–Villiger oxidation. To this purpose, Iqbal et al. [70] have reported the concurrent redox cascade to transform (-) carvone and (+)-carvone [(R) and (S)-26] into the correspondent lactones, as depicted in Scheme 11. Scheme 10. Preparation of enantiopure (R)-2-alkyl- δ -lactones by using a whole-cell-catalyzed cascade involving BVMO activity. In this example, the cascade catalysis was started with 50 mL of cell suspension of Acinetobacter sp. RS1 in Tris buffer (cell density 12 g cdw/L) containing 40 mg of alkylidene ketones 18 or 19 and 20 mg/mL glucose. After 3 h of reaction to allow the ERED-mediated accumulation of alkyl ketones 20 and 21 (R-configuration), 150 mL of Tris buffer containing engineered cells of E. coli (CHMO − GDH) (10 g cdw/L) was added to start the Baeyer– Villiger oxidation. After simultaneous oxidation and hydrolysis for 1.5 h, extraction with ethyl acetate and purification by flash chromatography, compounds (R)- 22 (56% yield, 98% ee) or (R)- 23 (41% yield, 97% ee) could be obtained. The presence of a hydrolytic activity inside Acinetobacter sp. RS1 cells was responsible for the hydrolysis of the intermediate (S)- 22 or (S)- 23 leading to δ -hydroxyacids 24 or 25, obtained with low enantioselectivity (E= 8–11). The different enantiomers of carvolactones (7-methyl-4-(prop-1-en-2-yl)oxepan-2- ones) are gaining importance as monomers for the preparation of polymeric thermoplastic elastomers (shape-memory polymers) and pressure-sensitive adhesive components [67–69] . These carvolactones can be obtained starting from biogenic carvones after a C=C reduction and a Baeyer–Villiger oxidation. To this purpose, Iqbal et al. [ 70 ] have reported the concurrent redox cascade to transform (-) carvone and (+)-carvone [(R) and (S)- 26 ] into the correspondent lactones, as depicted in Scheme 11. Catalysts 2021,11, 605 16 of 25 3.2. Parallel ADHs/BVMOs Cascades Apart from linear cascades, in which one reaction is conducted before the start of the subsequent reaction, parallel or tandem cascades, with both reactions occurring simultaneously, have been developed combining ADHs and BVMOs. One of the first examples of parallel cascades with BVMOs was shown in 2010. These isolated catalysts and ADHs have been employed in a parallel fashion for the simultaneous preparation of optically active compounds [ 95 ]. This methodology, called Parallel Interconnected Kinetic Asymmetric Transformations (PIKAT), allowed minimizing the quantity of reagents, thus maximizing the redox economy of the system. The PIKAT was performed by coupling two different asymmetric transformations: (a) via two kinetic resolutions (Scheme 17) or (b) via a kinetic resolution and a desymmetrization reaction (Scheme 18). Catalysts 2021, 11, x FOR PEER REVIEW 16 of 25 3.2. Parallel ADHs/BVMOs Cascades Apart from linear cascades, in which one reaction is conducted before the start of the subsequent reaction, parallel or tandem cascades, with both reactions occurring simultaneously, have been developed combining ADHs and BVMOs. One of the first examples of parallel cascades with BVMOs was shown in 2010. These isolated catalysts and ADHs have been employed in a parallel fashion for the simultaneous preparation of optically active compounds [95]. This methodology, called Parallel Interconnected Kinetic Asymmetric Transformations (PIKAT), allowed minimizing the quantity of reagents, thus maximizing the redox economy of the system. The PIKAT was performed by coupling two different asymmetric transformations: (a) via two kinetic resolutions (Scheme 17) or (b) via a kinetic resolution and a desymmetrization reaction (Scheme 18). Scheme 17. Parallel interconnected kinetic asymmetric transformation for the concurrent synthesis of optically active alcohols, ketones and esters combining ADHs and BVMOs in a double kinetic resolution. By the proper selection of both catalysts (an ADH and a BVMO, 2 U) and using a catalytic amount of NADPH, which acts as the connector between the two transformations, all possible enantiomers can be obtained in a one-pot process. Phenylacetone monooxygenase (PAMO) from Thermobifida fusca [96] and 4-hydroxyacetophenone monooxygenase (HAPMO) from Pseudomonas fluorescens ACB [97] were selected as BVMOs, due to their excellent behavior in the kinetic resolution of racemic ketones [98], whereas two NADPH-dependent ADHs with opposite selectivity were employed: LbADH and ADH from Thermoanaerobacter sp. (ADH-T). Initial experiments were carried out in the interconnected kinetic resolution of racemic 2-phenylpentan-3-one (43a) and 2- octanol (45a) with BVMOs and ADHs in presence of a catalytic amount of NADPH (0.2 mM). When using HAPMO and ADH-T at 20 °C and pH 7.5, it was possible to obtain (R)- 43a and (S)-44a in a process with excellent enantioselectivity (E > 200). ADH-T was able to oxidize (S)-45a to ketone 46a, remaining (R)-45a with high enantiomeric excess. When LbADH was used instead of ADH-T, (S)-45a was recovered in an excellent double kinetic resolution when the PIKAT was carried out at pH 8.5. PAMO was also tested in this process. When combined with ADH-T, (R)-43a, (S)-44a and (R)-45a were obtained with conversions around 50% and excellent enantioselectivities. The pH and the temperature effect were studied in the coupled system PAMO/LbADH, being observed that the system can work even at 60 °C, but the best results were achieved at pH 8.5 and 30 °C. A small amount of hexyl acetate (47a) was recovered in the reaction medium. This compound was obtained by the PAMO-catalyzed oxidation of 2-octanone 46a in a secondary reaction. The parallel resolution was extended to other secondary alcohols such as undecanol (45b) or sulcatol (45c) in combination with the BVMO-catalyzed resolution of rac-43a. For all the Scheme 17. Parallel interconnected kinetic asymmetric transformation for the concurrent synthesis of optically active alcohols, ketones and esters combining ADHs and BVMOs in a double kinetic resolution. Catalysts 2021, 11, x FOR PEER REVIEW 17 of 25 BVMOs and ADHs, excellent results were obtained in the resolution of racemic 45b and 43a, being observed that PAMO led to better conversions and enantioselectivities than with HAPMO. (R)-sulcatol was obtained with low optical purities in the ADH-T-biocata- lyzed reactions, as this enzyme was not able to perform a selective bio-oxidation of the racemic alcohol. When the reactions were carried out with LbADH, excellent resolutions were achieved. 4-Phenylhexan-3-one (rac-43b) was resolved in presence of 45a, with excellent selectivity and high optical purities for all the biocatalysts combinations, thus recovering (R)-43b, (S)-44b and (R)- or (S)-45a depending on the enzymes employed. In a further development of the PIKAT system, the kinetic resolution of racemic alcohols catalyzed by isolated ADHs was coupled with the desymmetrization of different prochiral sulfides employing BVMOs [99], as shown in Scheme 17. Scheme 18. Combination of ADHs and BVMOs for the kinetic resolution of racemic 2-octanol and the enantioselective sulfoxidation of prochiral sulfides in a concurrent fashion. Thus, racemic 2-octanol was selectively oxidized by both LBADH and ADH-T in a process coupled with the enantioselective oxidation of sulfides 48a–f catalyzed by PAMO, its M446G mutant [100] or HAPMO. In order to ensure the best performance for these biocatalysts, PAMO and its mutant were used at 30 °C whereas HAPMO was employed at 20 °C. Aromatic sulfoxides (49a–d) were obtained with moderate to good conversions and high optical purity, whereas the oxidation of rac-45a led to the (R)- or (S)-alcohol with high enantiomeric excess and conversions around 50%. Some amount of ester 44a is formed, due to the BVMO oxidation of ketone 43a. The system was also applied to the sulfoxidation of a heteroaryl alkyl sulfide (45e), being possible to obtain both enantiomers of the sulfoxide with high selectivity, whereas (S)-cyclohexyl methyl sulfoxide (49f) was obtained with excellent optical purity and high conversion in the system catalyzed by HAPMO and both of the ADHs. The concurrent synthesis of (S)-methyl phenyl sulfoxide (49a; R = Ph, n = 0) catalyzed by HAPMO was coupled with the kinetic resolution of several secondary alcohols in presence of LBADH, achieving excellent results in the oxidation of 2-octanol, 2-undecanol and sulcatol to yield the (S)-enantiomers. When the hydroxyl moiety was in position 3 of the starting alcohol (3-octanol) or diols were employed, lower optical purities were measured. Finally, the NADPH cofactor in the reaction medium was optimized, in order to ensure an effective large-scale application. Studies revealed that when the cofactor concentration was 5 μM the system performance was optimal in the concurrent sulfoxidation of thioanisole (48a) and the kinetic resolution of 2-octanol combining HAPMO and LBADH. Lower concentrations (1 μM) can be used in the double kinetic resolution of rac-43b and rac-45a catalyzed by PAMO and LBADH, respectively, as this ketone is a very good substrate for the BVMO. Scheme 18. Combination of ADHs and BVMOs for the kinetic resolution of racemic 2-octanol and the enantioselective sulfoxidation of prochiral sulfides in a concurrent fashion. By the proper selection of both catalysts (an ADH and a BVMO, 2 U) and using a catalytic amount of NADPH, which acts as the connector between the two transformations, all possible enantiomers can be obtained in a one-pot process. Phenylacetone monooxygenase (PAMO) from Thermobifida fusca [ 96 ] and 4-hydroxyacetophenone monooxygenase (HAPMO) from Pseudomonas fluorescens ACB [ 97 ] were selected as BVMOs, due to their excellent behavior in the kinetic resolution of racemic ketones [ 98 ], whereas two NADPH- dependent ADHs with opposite selectivity were employed: LbADH and ADH from Catalysts 2021,11, 605 17 of 25 Thermoanaerobacter sp. (ADH-T). Initial experiments were carried out in the interconnected kinetic resolution of racemic 2-phenylpentan-3-one ( 43a ) and 2-octanol ( 45a ) with BVMOs and ADHs in presence of a catalytic amount of NADPH (0.2 mM). When using HAPMO and ADH-T at 20 ◦ C and pH 7.5, it was possible to obtain (R)- 43a and (S)- 44a in a process with excellent enantioselectivity (E> 200). ADH-T was able to oxidize (S)- 45a to ketone 46a , remaining (R)- 45a with high enantiomeric excess. When LbADH was used instead of ADH-T, (S)- 45a was recovered in an excellent double kinetic resolution when the PIKAT was carried out at pH 8.5. PAMO was also tested in this process. When combined with ADH-T, (R)- 43a , (S)- 44a and (R)- 45a were obtained with conversions around 50% and excellent enantioselectivities. The pH and the temperature effect were studied in the coupled system PAMO/LbADH, being observed that the system can work even at 60 ◦ C, but the best results were achieved at pH 8.5 and 30 ◦ C. A small amount of hexyl acetate ( 47a ) was recovered in the reaction medium. This compound was obtained by the PAMO-catalyzed oxidation of 2-octanone 46a in a secondary reaction. The parallel resolution was extended to other secondary alcohols such as undecanol ( 45b ) or sulcatol ( 45c ) in combination with the BVMO-catalyzed resolution of rac- 43a . For all the BVMOs and ADHs, excellent results were obtained in the resolution of racemic 45b and 43a , being observed that PAMO led to better conversions and enantioselectivities than with HAPMO. (R)-sulcatol was obtained with low optical purities in the ADH-T-biocatalyzed reactions, as this enzyme was not able to perform a selective bio-oxidation of the racemic alcohol. When the reactions were carried out with LbADH, excellent resolutions were achieved. 4-Phenylhexan-3-one (rac- 43b ) was resolved in presence of 45a , with excellent selectivity and high optical purities for all the biocatalysts combinations, thus recovering (R)- 43b , (S)- 44b and (R)- or (S)- 45a depending on the enzymes employed. In a further development of the PIKAT system, the kinetic resolution of racemic alcohols catalyzed by isolated ADHs was coupled with the desymmetrization of different prochiral sulfides employing BVMOs [ 99 ], as shown in Scheme 17. Thus, racemic 2-octanol was selectively oxidized by both LBADH and ADH-T in a process coupled with the enantioselective oxidation of sulfides 48a – f catalyzed by PAMO, its M446G mutant [ 100 ] or HAPMO. In order to ensure the best performance for these biocatalysts, PAMO and its mutant were used at 30 ◦ C whereas HAPMO was employed at 20 ◦ C. Aromatic sulfoxides ( 49a – d ) were obtained with moderate to good conversions and high optical purity, whereas the oxidation of rac- 45a led to the (R)- or (S)-alcohol with high enantiomeric excess and conversions around 50%. Some amount of ester 44a is formed, due to the BVMO oxidation of ketone 43a. The system was also applied to the sulfoxidation of a heteroaryl alkyl sulfide (45e), being possible to obtain both enantiomers of the sulfoxide with high selectivity, whereas (S)-cyclohexyl methyl sulfoxide ( 49f ) was obtained with excellent optical purity and high conversion in the system catalyzed by HAPMO and both of the ADHs. The concurrent synthesis of (S)-methyl phenyl sulfoxide ( 49a ; R = Ph, n = 0) catalyzed by HAPMO was coupled with the kinetic resolution of several secondary alcohols in presence of LBADH, achieving excellent results in the oxidation of 2-octanol, 2-undecanol and sulcatol to yield the (S)-enantiomers. When the hydroxyl moiety was in position 3 of the starting alcohol (3-octanol) or diols were employed, lower optical purities were measured. Finally, the NADPH cofactor in the reaction medium was optimized, in order to ensure an effective large-scale application. Studies revealed that when the cofactor concentration was 5 µ M the system performance was optimal in the concurrent sulfoxidation of thioanisole ( 48a ) and the kinetic resolution of 2-octanol combining HAPMO and LBADH. Lower concentrations (1 µ M) can be used in the double kinetic resolution of rac- 43b and rac- 45a catalyzed by PAMO and LBADH, respectively, as this ketone is a very good substrate for the BVMO. The production of ε -caprolactone combining isolated BVMOs and ADHs has also been described through a convergent cascade [ 101 ], depicted in Scheme 19. In this approach, two different substrates were employed, which leads to the final product without the formation of any intermediate. Catalysts 2021,11, 605 18 of 25 Catalysts 2021, 11, x FOR PEER REVIEW 18 of 25 The production of ε-caprolactone combining isolated BVMOs and ADHs has also been described through a convergent cascade [101], depicted in Scheme 19. In this approach, two different substrates were employed, which leads to the final product without the formation of any intermediate. Scheme 19. Convergent cascade for the synthesis of ECL starting from cyclohexanone and 1,6- hexanodiol employing BVMOs (AcCHMO) and ADHs (TeSADH). Thus, two equivalents of cyclohexanone 8 were oxidized by a BVMO (0.1–1.0 mg/mL) to caprolactone 9, whereas the ADH-catalyzed oxidation of one equivalent of 1,6-hex- anediol 50 afforded the same lactone, with simultaneous regeneration of the NAD(P)H cofactor, as shown in Scheme 18. Biocatalysts were employed as crude extracts free of cells. The initial study for finding the best ADH in this oxidation pointed towards the Thermus sp. ATN1 ADH (TADH) as a promising candidate for this reaction. Unfortunately, this biocatalyst is NADH dependent and no suitable BVMOs with preference for this cofactor were found for the cascade proposed. Then, different bienzymatic systems were tested, achieving the highest concentration of final lactone when combining AcCHMO with TeSADH (0.01–0.1 mg/mL), a NADPH-dependent biocatalyst. Thus, 10 mM of ECL were formed when starting from 20 mM of cyclohexanone and 10 mM of 1,6- hexanediol. Reactions in absence of one or two of the catalytic members (cofactor, CHMO and ADH) were carried out. ECL was obtained in 6 mM concentration after 72 h in absence of TeSADH, indicating that some endogenous ADH presented in the crude CHMO preparation can present some activity on 50. A very low concentration of ECL was recovered (2 mM) in absence of NADPH, maybe owing to the cofactor present in the extracts. No lactone formation was observed in absence of CHMO. In addition, some ECL hydrolysis was observed in the reaction conditions. The CHMO-TeSADH system was optimized by increasing the concentration of substrates and biocatalysts. Addition of extra CHMO after 48 h allowed obtaining complete conversion after 72 h, whereas doubling the starting amount of enzymes led to the same conversion after 48 h, but with a lower turnover frequency (2.0 vs. 4.8 h−1). Increasing cyclohexanone concentration to 100 mM led to the same TOF (1.8 h−1) with only 32% conversion after 72 h. The study of the reaction showed that cyclohexanol was formed during the process as a by-product, achieving a maximum value after 24 h, and then decreasing its concentration until it disappearance at 72 h. The convergent cascade was run at 50 mL scale, starting with 98 mg of ketone (20 mM) and 59 mg of diol (10 mM). After 18 h, complete cyclohexanone conversion was observed, with 19.7 mM of ECL. This result can be debt to a better oxygenation in the multimilligram scale. After 24 h, lactone concentration was 19 mM and 3.7 mM of diol were measured. Reaction work up afforded 150 mg of a mixture containing ECL, poly-ECL and diol at 1:1.5:0.6 ratio. 3.3. Sequential EREDs/BVMOs Cascades Issa et al. [102] have recently studied the enzymatic bioreduction of synthetic analogies of (-)-carvone (R)-13 (Scheme 5) decorated with different substituents at position C3 and/or C6, and their subsequent transformation into the corresponding carvolactones by means of BVMOs, as shown in Scheme 20. Scheme 19. Convergent cascade for the synthesis of ECL starting from cyclohexanone and 1,6- hexanodiol employing BVMOs (AcCHMO) and ADHs (TeSADH). Thus, two equivalents of cyclohexanone 8 were oxidized by a BVMO (0.1–1.0 mg/mL) to caprolactone 9 , whereas the ADH-catalyzed oxidation of one equivalent of 1,6-hexanediol 50 afforded the same lactone, with simultaneous regeneration of the NAD(P)H cofactor, as shown in Scheme 18. Biocatalysts were employed as crude extracts free of cells. The initial study for finding the best ADH in this oxidation pointed towards the Thermus sp. ATN1 ADH (TADH) as a promising candidate for this reaction. Unfortunately, this biocatalyst is NADH dependent and no suitable BVMOs with preference for this cofactor were found for the cascade proposed. Then, different bienzymatic systems were tested, achieving the highest concentration of final lactone when combining AcCHMO with TeSADH ( 0.01–0.1 mg/mL ), a NADPH-dependent biocatalyst. Thus, 10 mM of ECL were formed when starting from 20 mM of cyclohexanone and 10 mM of 1,6-hexanediol. Reactions in absence of one or two of the catalytic members (cofactor, CHMO and ADH) were carried out. ECL was obtained in 6 mM concentration after 72 h in absence of TeSADH, indicating that some endogenous ADH presented in the crude CHMO preparation can present some activity on 50 . A very low concentration of ECL was recovered (2 mM) in absence of NADPH, maybe owing to the cofactor present in the extracts. No lactone formation was observed in absence of CHMO. In addition, some ECL hydrolysis was observed in the reaction conditions. The CHMO-TeSADH system was optimized by increasing the concentration of substrates and biocatalysts. Addition of extra CHMO after 48 h allowed obtaining complete conversion after 72 h, whereas doubling the starting amount of enzymes led to the same conversion after 48 h, but with a lower turnover frequency (2.0 vs. 4.8 h −1 ). Increasing cyclohexanone concentration to 100 mM led to the same TOF (1.8 h −1 ) with only 32% conversion after 72 h. The study of the reaction showed that cyclohexanol was formed during the process as a by-product, achieving a maximum value after 24 h, and then decreasing its concentration until it disappearance at 72 h. The convergent cascade was run at 50 mL scale, starting with 98 mg of ketone (20 mM) and 59 mg of diol (10 mM). After 18 h, complete cyclohexanone conversion was observed, with 19.7 mM of ECL. This result can be debt to a better oxygenation in the multimilligram scale. After 24 h, lactone concentration was 19 mM and 3.7 mM of diol were measured. Reaction work up afforded 150 mg of a mixture containing ECL, poly-ECL and diol at 1:1.5:0.6 ratio. 3.3. Sequential EREDs/BVMOs Cascades Issa et al. [ 102 ] have recently studied the enzymatic bioreduction of synthetic analogies of (-)-carvone (R)- 13 (Scheme 5) decorated with different substituents at position C3 and/or C6, and their subsequent transformation into the corresponding carvolactones by means of BVMOs, as shown in Scheme 20. Catalysts 2021,11, 605 19 of 25 Catalysts 2021, 11, x FOR PEER REVIEW 19 of 25 Scheme 20. Enzymatic sequential conversion of synthetic analogues of carvones 51 into dihydrocarvones 52 and carvolactones 53. EREDs tested (2–10 μm), were OYE2 and OYE3 [103] from S. cerevisiae and PETNR [82,83], while CHMO_Ph1 [84] was used for the Baeyer–Villiger oxidation. Data provided by these authors [102] showed that the configuration of the extra methyl (R1) at C6 did not affect the binding and/or orientation of the substrate in the bioreduction compared to the parent compound (R)-13. Thus, (6S) stereochemistry of 51a was always leading to (2S) stereoisomers of 52a, and (6R) isomers of 51a were furnishing (2R) stereoisomers of 51a. Notably, the stereo-configuration of the methyl at C6 does significantly impact the rate of conversion; in fact, (6S)-51a was converted more slowly and to a lower yield (15% with OYE2 in 24 h, 17% with PETNR in 24 h) compared to (6R)-51a (90% with OYE2 in 2 h, 88% with PETNR at the same time). This reduction in conversion upon transforming the pure diastereomers (5R,6S)-51a and (5R,6R)-51a was also observed using a mixture of them, where a more rapid depletion of (5R,6R)-51a and a quick formation of (2R,3R,6R)-52a was reported. Remarkably, a computational model to understand this stereochemical pattern was proposed [102]. In a second step, the effect of heteroatom substitution at C6 was explored, using 6- OH substitution instead of 6-Me (substrates 51b). In this case, (5S,6S)-51b and (5S,6R)-51b were respectively converted to the corresponding 6-hydroxydihydrocarvones (2S,3S,6R)- 52b and (2R,3S,6R)-52b, by OYE2 with moderate yields (40% and 30% after 24 h) but with very high diastereomeric excesses (≥99% de) in all cases. On the other hand, PETNR performed better, affording 66 and 80% yields of (2S,3S,6R)-52b and (2R,3S,6R)-52b, respectively, after 2 h, also with ≥99% de. As with the 6-methyl derivatives, these were poorer substrates for OYE3 under the same conditions. The corresponding 6-hydroxycarvone diastereoisomers derived from (+)-carvone (S)-13, (5R,6S)-51b and (5R,6R)-51b, were also poor substrates for OYE2, affording <10% yields at 2–24 h. A similar behavior was reported with PETNR on the (5R,6S)-51b (5–10% maximum yield), although this enzyme allowed yields (85–95%) of (2R,3R,6R)-52b when starting from (5R,6R)-51b, but with much reduced de (<30%). Finally, synthetic C3-Me-(-)-carvone analogue 51c is a regioisomer substrate of 51a, but introducing a methyl at the site of enzymatic conjugate reductive attack. Bioreductions catalyzed by OYE2 and PETNR proceeded with low yields (≤ 10%), although with high diastereoselectivity (de ≥ 99) for compound (2S,3R,6R)-52c. After this exhaustive stereochemical study of the EREDs performance, Issa et al. [102] assessed the BV oxidation with the different diastereomers of 52a and 52b. The (2R,3R,6R)- 52a isomer was completely converted to lactone (3R,6S,7R)-53a (oxygen insertion between C1–C2), leading to with apparently complete regioselectivity (≥99%), with no traces of the lactone coming from the oxygen insertion between C1-C6. Remarkably, there was no reaction at all when starting with (2S,3R,6R)-52a; this indicates a remarkable diastereoisomer-selectivity, as an inversion of 6-methyl absolute configuration avoids the enzymatic transformation. Thus, a cascade reaction was carried out at 50 mg scale, as shown in Scheme 21. Scheme 20. Enzymatic sequential conversion of synthetic analogues of carvones 51 into dihydrocarvones 52 and carvolactones 53. EREDs tested (2–10 µ m), were OYE2 and OYE3 [ 103 ] from S. cerevisiae and PETNR [82,83] , while CHMO_Ph1 [ 84 ] was used for the Baeyer–Villiger oxidation. Data provided by these authors [ 102 ] showed that the configuration of the extra methyl (R 1 ) at C6 did not affect the binding and/or orientation of the substrate in the bioreduction compared to the parent compound (R)- 13 . Thus, (6S) stereochemistry of 51a was always leading to (2S) stereoisomers of 52a , and (6R) isomers of 51a were furnishing (2R) stereoisomers of 51a . Notably, the stereo-configuration of the methyl at C6 does significantly impact the rate of conversion; in fact, (6S)- 51a was converted more slowly and to a lower yield (15% with OYE2 in 24 h, 17% with PETNR in 24 h) compared to (6R)- 51a (90% with OYE2 in 2 h, 88% with PETNR at the same time). This reduction in conversion upon transforming the pure diastereomers (5R,6S)- 51a and (5R,6R)- 51a was also observed using a mixture of them, where a more rapid depletion of (5R,6R)- 51a and a quick formation of (2R,3R,6R)- 52a was reported. Remarkably, a computational model to understand this stereochemical pattern was proposed [102]. In a second step, the effect of heteroatom substitution at C6 was explored, using 6-OH substitution instead of 6-Me (substrates 51b ). In this case, (5S,6S)- 51b and (5S,6R)- 51b were respectively converted to the corresponding 6-hydroxydihydrocarvones (2S,3S,6R)- 52b and (2R,3S,6R)- 52b , by OYE2 with moderate yields (40% and 30% after 24 h) but with very high diastereomeric excesses ( ≥ 99% de) in all cases. On the other hand, PETNR performed better, affording 66 and 80% yields of (2S,3S,6R)- 52b and (2R,3S,6R)- 52b , respectively, after 2 h, also with ≥ 99% de. As with the 6-methyl derivatives, these were poorer substrates for OYE3 under the same conditions. The corresponding 6-hydroxycarvone diastereoisomers derived from (+)-carvone (S)- 13 , (5R,6S)- 51b and (5R,6R)- 51b , were also poor substrates for OYE2, affording <10% yields at 2–24 h. A similar behavior was reported with PETNR on the (5R,6S)- 51b (5–10% maximum yield), although this enzyme allowed yields (85–95%) of (2R,3R,6R)- 52b when starting from (5R,6R)- 51b , but with much reduced de (<30%). Finally, synthetic C3-Me-(-)-carvone analogue 51c is a regioisomer substrate of 51a , but introducing a methyl at the site of enzymatic conjugate reductive attack. Bioreductions catalyzed by OYE2 and PETNR proceeded with low yields ( ≤ 10%), although with high diastereoselectivity (de ≥99) for compound (2S,3R,6R)-52c. After this exhaustive stereochemical study of the EREDs performance, Issa et al. [ 102 ] assessed the BV oxidation with the different diastereomers of 52a and 52b . The (2R,3R,6R)- 52a isomer was completely converted to lactone (3R,6S,7R)- 53a (oxygen insertion between C1–C2), leading to with apparently complete regioselectivity ( ≥ 99%), with no traces of the lactone coming from the oxygen insertion between C1-C6. Remarkably, there was no reaction at all when starting with (2S,3R,6R)- 52a ; this indicates a remarkable diastereoisomer-selectivity, as an inversion of 6-methyl absolute configuration avoids the enzymatic transformation. Thus, a cascade reaction was carried out at 50 mg scale, as shown in Scheme 21. Catalysts 2021,11, 605 20 of 25 Catalysts 2021, 11, x FOR PEER REVIEW 20 of 25 Scheme 21. Enzymatic sequential conversion of (5R,6R)-51a into dihydrocarvone (2R,3R,6R)-52a and carvolactone (3R,6S,7R)-53a. Regarding 3-methyl modified dihydrocarvones 52c, they were chemically synthesized staring from (R)-13, as shown in Scheme 22. This chemical reduction with CuBr and trimethylaluminium was carried out because of the poor ERED outcome using OYE2 and PETNR on 51c, so that a mixture of four isomers with a diastereomeric ratio of 1.5:1:1:0.1 was obtained. Scheme 22. Chemical synthesis of dihydrocarvones 49c and subsequent enzymatic Baeyer–Villiger oxidation to carvolactones 50c. The two 3R diastereomers ((2S,3R,5R)-52c and (2R,3R,5R)-52c, about 70–75% of total) were separated from the two (3S)-isomers, so that it was possible to evaluate valuation of all four isomers, and of the separate pairs of C3 diastereomers with CHMO_Phi1. Bioreductions were run at 25 °C for 24 h, with the system NADP+/GDH employed as the hydride donor, leading to a complete conversion of (2S,3R,5R)-52c and (2R,3R,5R)-52c to their corresponding enantiopure lactones (3S,4R,6S)-53c and (3R,4R,6S)-53c in 98% yield (Scheme 21). This process was scaled up to 50 mg. However, no lactone formation was observed from the bio-oxidation of (2R,3S,5R)-52c and (2S,3S,5R)-52c diastereomers. 4. Conclusions In the last few years, the development of multienzymatic processes has been largely spread, being possible to design novel biotransformations avoiding the isolation of reaction intermediates and reducing the consumption of solvents and energy, thus maximizing the atom economy of the reactions. For this reason, several processes in which two or more biocatalysts are employed in a sequential or concurrent fashion have been designed. Among these biocatalysts, Baeyer–Villiger monooxygenases have been demonstrated to be useful biocatalysts for the synthesis of valuable compounds employing mild and environmentally friendly conditions. Thus, these enzymes have been employed in multienzymatic reactions in combination with other oxidoreductases, mainly coupled to enoate reductases or alcohol dehydrogenases, being described in some examples in which cytochrome P450 are also involved. EREDs have been generally employed for the hydrogenation of α,β-unsaturated cycloketones to the corresponding cyclic ketones, which are then oxidized to valuable lactones. When combined with alcohol dehydrogenases, most of the synthetic procedures described balance redox systems in which the nicotinamide co-factor required by both biocatalysts is recycled through an initial ADH-catalyzed oxidation of an alcohol to a ketone, with a subsequent BVMO-catalyzed formation of ester or lactone. Most of these examples Scheme 21. Enzymatic sequential conversion of (5R,6R)- 51a into dihydrocarvone (2R,3R,6R)- 52a and carvolactone (3R,6S,7R)-53a. Regarding 3-methyl modified dihydrocarvones 52c , they were chemically synthesized staring from (R)- 13 , as shown in Scheme 22. This chemical reduction with CuBr and trimethylaluminium was carried out because of the poor ERED outcome using OYE2 and PETNR on 51c , so that a mixture of four isomers with a diastereomeric ratio of 1.5:1:1:0.1 was obtained. Catalysts 2021, 11, x FOR PEER REVIEW 20 of 25 Scheme 21. Enzymatic sequential conversion of (5R,6R)-51a into dihydrocarvone (2R,3R,6R)-52a and carvolactone (3R,6S,7R)-53a. Regarding 3-methyl modified dihydrocarvones 52c, they were chemically synthesized staring from (R)-13, as shown in Scheme 22. This chemical reduction with CuBr and trimethylaluminium was carried out because of the poor ERED outcome using OYE2 and PETNR on 51c, so that a mixture of four isomers with a diastereomeric ratio of 1.5:1:1:0.1 was obtained. Scheme 22. Chemical synthesis of dihydrocarvones 49c and subsequent enzymatic Baeyer–Villiger oxidation to carvolactones 50c. The two 3R diastereomers ((2S,3R,5R)-52c and (2R,3R,5R)-52c, about 70–75% of total) were separated from the two (3S)-isomers, so that it was possible to evaluate valuation of all four isomers, and of the separate pairs of C3 diastereomers with CHMO_Phi1. Bioreductions were run at 25 °C for 24 h, with the system NADP+/GDH employed as the hydride donor, leading to a complete conversion of (2S,3R,5R)-52c and (2R,3R,5R)-52c to their corresponding enantiopure lactones (3S,4R,6S)-53c and (3R,4R,6S)-53c in 98% yield (Scheme 21). This process was scaled up to 50 mg. However, no lactone formation was observed from the bio-oxidation of (2R,3S,5R)-52c and (2S,3S,5R)-52c diastereomers. 4. Conclusions In the last few years, the development of multienzymatic processes has been largely spread, being possible to design novel biotransformations avoiding the isolation of reaction intermediates and reducing the consumption of solvents and energy, thus maximizing the atom economy of the reactions. For this reason, several processes in which two or more biocatalysts are employed in a sequential or concurrent fashion have been designed. Among these biocatalysts, Baeyer–Villiger monooxygenases have been demonstrated to be useful biocatalysts for the synthesis of valuable compounds employing mild and environmentally friendly conditions. Thus, these enzymes have been employed in multienzymatic reactions in combination with other oxidoreductases, mainly coupled to enoate reductases or alcohol dehydrogenases, being described in some examples in which cytochrome P450 are also involved. EREDs have been generally employed for the hydrogenation of α,β-unsaturated cycloketones to the corresponding cyclic ketones, which are then oxidized to valuable lactones. When combined with alcohol dehydrogenases, most of the synthetic procedures described balance redox systems in which the nicotinamide co-factor required by both biocatalysts is recycled through an initial ADH-catalyzed oxidation of an alcohol to a ketone, with a subsequent BVMO-catalyzed formation of ester or lactone. Most of these examples Scheme 22. Chemical synthesis of dihydrocarvones 49c and subsequent enzymatic Baeyer–Villiger oxidation to carvolactones 50c. The two 3Rdiastereomers ((2S,3R,5R)- 52c and (2R,3R,5R)- 52c , about 70–75% of total) were separated from the two (3S)-isomers, so that it was possible to evaluate valuation of all four isomers, and of the separate pairs of C3 diastereomers with CHMO_Phi1. Bioreductions were run at 25 ◦ C for 24 h, with the system NADP + /GDH employed as the hydride donor, leading to a complete conversion of (2S,3R,5R)- 52c and (2R,3R,5R)- 52c to their corresponding enantiopure lactones (3S,4R,6S)- 53c and (3R,4R,6S)- 53c in 98% yield (Scheme 21). This process was scaled up to 50 mg. However, no lactone formation was observed from the bio-oxidation of (2R,3S,5R)-52c and (2S,3S,5R)-52c diastereomers. 4. Conclusions In the last few years, the development of multienzymatic processes has been largely spread, being possible to design novel biotransformations avoiding the isolation of reaction intermediates and reducing the consumption of solvents and energy, thus maximizing the atom economy of the reactions. For this reason, several processes in which two or more biocatalysts are employed in a sequential or concurrent fashion have been designed. Among these biocatalysts, Baeyer–Villiger monooxygenases have been demonstrated to be useful biocatalysts for the synthesis of valuable compounds employing mild and environmentally friendly conditions. Thus, these enzymes have been employed in multienzymatic reactions in combination with other oxidoreductases, mainly coupled to enoate reductases or alcohol dehydrogenases, being described in some examples in which cytochrome P450 are also involved. EREDs have been generally employed for the hydrogenation of α , β -unsaturated cycloketones to the corresponding cyclic ketones, which are then oxidized to valuable lactones. When combined with alcohol dehydrogenases, most of the synthetic procedures described balance redox systems in which the nicotinamide co-factor required by both biocatalysts is recycled through an initial ADH-catalyzed oxidation of an alcohol to a ketone, Catalysts 2021,11, 605 21 of 25 with a subsequent BVMO-catalyzed formation of ester or lactone. Most of these examples involved the preparation of ε -caprolactone or some of its analogues from cyclohexanols in a bienzymatic transformation combining the ADH-catalyzed oxidation of the alcohol with the BVMO-catalyzed oxidation of the ketone with in situ cofactor regeneration. This model system has been studied by employing both whole-cell and cell-free systems, but, in all the cases, a critical parameter is the low operational stability of the Baeyer–Villiger monooxygenase, very sensitive to the substrate and product concentration or to the reaction conditions. Different approaches have been made to overcome this drawback, including the application of more stable biocatalysts able to tolerate higher concentrations of substrates and products, the use of organic co-solvents or the inclusion of a further biocatalytic step with the aim of diminishing the ECL concentration in the reaction medium, as the lipase-catalyzed conversion of ECL to oligo-ECL or 6-hydroxyhexanoic acid. After all of these improvements, the results obtained indicate the requirement of further developments are still required to ensure the productive biocatalytic synthesis of ECL starting from cyclohexanol. Author Contributions: Conceptualization, G.d.G. and A.R.A.; writing—original draft preparation, G.d.G. and A.R.A.; writing—review and editing, G.d.G. and A.R.A.; visualization, G.d.G. and A.R.A.; supervision, G.d.G. and A.R.A. All authors have read and agreed to the published version of the manuscript. Funding: This research was partly funded by Spanish Ministry of Science and Innovation (PID2019- 105337RB-C22) and Banco de Santander-Complutense Research Projects (PR87/19-22676). Conflicts of Interest: The authors declare no conflict of interest. References 1. Kitano, H. Systems biology: A brief overview. Science 2002,295, 1662–1664. [CrossRef] 2. García-Junceda, E. Multi-Step Enzyme Catalysis: Biotransformations and Chemoenzymatic Synthesis; Wiley-VCH Verlag GmbH & Co. KGaA: Weinheim, Germany, 2008. [CrossRef] 3. García-Junceda, E.; Lavandera, I.; Rother, D.; Schrittwieser, J.H. (Chemo)enzymatic cascades-Nature’s synthetic strategy transferred to the laboratory. J. Mol. Catal. 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