Draft Genome Sequence of Pseudomonas fluorescens Strain ET76, Isolated from Rice Rhizosphere in Northwestern Morocco

D a Genome Sequence o Pseudomonas luo escens S ain ET76, Isola ed om Rice Rhizosphe e in No hwes e n Mo occo Saida Aa ab, a Abdelhay A ak ak, a F ancisco Ja ie Olle o, b Manuel Megías, b Douglas Fabiano Gomes, c Renan Augus o Ribei o, d Ma iangela Hung ia c,d Equipe de Reche che de Bio echnologies e Génie des Biomolécules, Facul é des Sciences e Techniques de Tange , Tange , Mo occo a ; Depa amen o de Mic obiología, Facul ad de Biología, Uni e sidad de Se illa, Se illa, Spain b ; Emb apa Soja, Soil Bio echnology, Lond ina, Pa aná, B azil c ; Conselho Nacional de Desen ol imen o Cien í ico e Tecnológico, B asília, Dis i o Fede al, B azil d Pseudomonas luo escens ET76 was isola ed om ice hizosphe e in no hwes e n Mo occo. I s d a genome was es ima ed o be 6,681,652 bp wi h 5,789 coding sequences (CDSs). Genes encoding o ype I o VI sec e ion sys ems, P dQ, p o eases, side opho es, hyd ogen cyanide syn hase, ACC-deaminase, among o he s, highligh i s po en ial use in biological con ol o plan pa hogens. Recei ed 18 Ma ch 2016 Accep ed 8 Ap il 2016 Published 19 May 2016 Ci a ion Aa ab S, A ak ak A, Olle o FJ, Megías M, Gomes DF, Ribei o RA, Hung ia M. 2016. D a genome sequence o Pseudomonas luo escens s ain ET76, isola ed om ice hizosphe e in no hwes e n Mo occo. Genome Announc 4(3):e00356-16. doi:10.1128/genomeA.00356-16. Copy igh © 2016 Aa ab e al. This is an open-access a icle dis ibu ed unde he e ms o he C ea i e Commons A ibu ion 4.0 In e na ional license. Add ess co espondence o Ma iangela Hung ia, [email p o ec ed] Pseudomonas luo escens s ain ET76 was isola ed om hizo- sphe e o ice (O yza sa i a L.) in paddies in no hwes e n Mo occo. ET76 has ypical p ope ies o plan g ow h-p omo ing bac e ia (PGPB), including he syn hesis o ACC-deaminase and side opho es and he capaci y o solubilizing phospha e (abou 600 mg L ⫺1 ), in addi ion o a s ong p o eoly ic ac i i y in i o. The bio echnological p ope ies o ET76 a e highligh ed by i s an agonis ac i i y o impo an plan pa hogens, including bo h bac e ia (Pseudomonas sa as anoi, Cla ibac e michiganensis,Xan- homonas campes is a . ci i, and Rals onia solanacea um) and ungi (Fusa ium oxyspo um,Phy oph ho a cac o um,Phy oph- ho a cinnamomi,Bo yo inia uckeliana,Ve icillium dahliae, and Colle o ichum acu a um); he e o e, he s ain has he po en ial o be used as an inoculan (1). To access he bac e ial genome sequence o ET76, o al DNA was ex ac ed using he DNeasy blood and issue ki (Qiagen) and p o- cessed on he MiSeq sequencing sys em (Illumina) a Emb apa Soja, Lond ina, B azil. Sho gun sequencing gene a ed a genome co e age o abou 19- old. Sequences we e submi ed o he Rapid Anno a ion using Subsys em Technology (RAST) se e (2) and he genome was es ima ed o be 6,681,652 bp, assembled in 68 con igs. Anno a ion in RAST iden i ied 5,789 coding sequences (CDSs), o which 52% we e classi ied in 545 subsys ems. Mos o he ca ego ies wi h CDSs classi- ied in o subsys ems we e amino acids and de i a i es (15.7%); ca - bohyd a es (11.2%); and co ac o s, i amins, p os he ic g oups, and pigmen s (8.2%). The highes genome simila i y was ound wi h Pseudomonas luo escens s ain Q8 1-96. Genes encoding o ype I, II, III, IV, V, VI, and VIII sec e ion sys ems and o he acylase P dQ in ol ed in quo um quenching we e de ec ed, bu no o AHL syn hase. No ewo hy was he high numbe o p o eases, such as sub ilin-like se ine p o ease, collagenase and ela ed p o eases, and me allo p o eases and cys eine p o eases; in addi ion, he e we e se e al lipases and phospholipases. In ela ion o he chemo axis and mo ili y ca ego y, he e a e 202 genes (4.2% o hose classi ied in subsys ems), including genes o swi ching mo ili y suchaspilHGT.ET76alsoca iesala genumbe o genes(221,5.0%) ela ed o s ess esponse, 95 o which a e in ol ed in oxida i e s ess. I has been epo ed ha he an imic obial ac i i y o Pseudomo- nas spp. may be ela ed o he syn hesis o me aboli es such as 2, 4-diace ylphlo oglucinol (2, 4-DAPG), py olni in, pyolu eo in, o hyd ogen cyanide (3,4). The genome o ET76 ca ies genes ela ed o he biosyn hesis o py oloquinoline quinone, phenazine, he side o- pho e pyo e dine, and o hyd ogen cyanide syn hase (hcnABC genes). The p esence o hese molecules could explain he an agonis ac i i y o ET76 o pa hogenic bac e ia and ungi, and e eals a g ea po en ial o use as a biological con ol agen . In addi ion, we con- i med ha he genome ca ies genes coding o he enzyme ACC(1- aminocyclop opane-1-ca boxyla e)-deaminase, which me abolizes ACC, a p ecu so o he e hylene biosyn hesis in highe plan s, which could help plan g ow h and de elopmen unde s ess condi ions by educing he s ess ela ed o e hylene (5,6). Nucleo ide sequence accession numbe s. This whole-genome sho gun p ojec has been deposi ed a DDBJ/EMBL/GenBank unde he accession numbe LNAB00000000, SUBID SUB1165101, Bio- P ojec PRJNA301338, BioSample SAMN04244127, e sion 02. ACKNOWLEDGMENTS This wo k was unded by he p ojec AGL2012-38831 o he Minis e io de Economía y Compe i i idad om he Spanish go e nmen and by CNPq, p ojec 470515/2012-0. FUNDING INFORMATION This wo k, including he e o s o Manuel Megías, was unded by Spanish Go e nmen , Minis e io de Economia y Compe i i idad (AGL2012 -38831). 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