Chromosome numbers of plants collected during Iter Mediterraneum IV in Cyprus
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B occo n ea I I - 1 99<) 11 7 Chromosome numbers of plants collected during Iter Mediterraneum IV in Cyprus Robert Vogt & Abela rd o Aparicio Introduction Th e fOllrth OPT IMA expedition, Iter Mediterranellm IV (Cypr ll s, Aprii 1 99 1) r es ulted in th e co ll ec ti on of c. 1 000 numb ers of vasc ul ar pl ants. During th e exc ursion th e a uÙlOr s co ll ec ted seed mate ri al and fixed now er bud s for the purpo se of kar yo l og i ca l studies. Th ey su ccee ded in study in g I 12 t axa. belonging to 22 fami li es a nd 85 genera. Th e r es ults are su mm arized here. and pr ese nt one of th e first extensive karyo logical contributions to tb e nora of Cyp rus. Material and me thod s The se ed material and the fixations of f1 0we r buds u se d for this stucly were co ll ec ted during th e 4th OPTTMA expedition to Cyp rus. Samp l es are indi ca ted in the te x! and in Ta bl e I by ùl e l oca li ty pllls co ll ec ti on nllmb er of Iter Me dit er raneum IV. Add itional seed mate ri al a ncl fl ower buds ga ùl er ecl without a co rr es pondin g 'official" co ll ec ti on numb er is cit ed with th c pers ona l co ll ec ti on numb er of th e autbors , aga in pr cede d by the l oca lity numb er. Herbarium vouchers are depo s it ed . in SEVF (Apa ri cio) and 13 ( Vo gt), r es p ec ti vely. Studi es on dip loid c hrom oso me numb ers were ca rried out in the Botanic Ga rden and Botanical Musellm Be rlin -Da hlem. Plants fo r karyol og ical study were rai se d at th e Berlin Bota ni c Ga rden and vO ll chers of cultivatecl plants are deposited in th e h er barium of th e Bota ni cal Mlisellm (B ). C hrom oso rn e numb ers have be en ob tained from s omati c mitoses of r oo t tips of pIanI. raised from seed. R oo t tips wer e pretr ea ted with hydroxychino li ne (0. 002 molar aqu eo us so lu ti on) fo r 2 hours, fixed in 96 % et hanol/glacial acet ic ac icl (3: I) a ncl r ef ri gerated. H ydro lyzation was ca rriecl out wilh 1 -2 n h ydroc hloric acid for 10 -15 minut es al 60 °C. For c hrom os om e staining rool tips wer e squashed in aceto -o rce in . C hr om osome cO llnts we re made from s eve ral plants of the same o ri gino Wh en possible five lO len metaphase . t ages were exa mined for eac h plant. Studi es on haploi cl c hr o mo so me nu mbers from yo ung n owe r buds fi xed in 96% elhanol/glacial acetic ac icl (3: I) were ca rrie cl out mainly in th e Department of Pi a nI Bi ol ogy and Ecol ogy, Sevilla University. The a nÙl ers we re stain cl in co lcl alcoho li c ac icl carmin e ( Snow 1 963) . Fo r m os l of th e exami n ed t axa a pho l. ogra ph 01' th e mil.olic or meiotic kar yo type is pro vi dcd.
118 Vogt & Aparici o: Chromosome numbers of pla nt s coll ec ted ... Table 1. Chromosome eounls 01 phanerogams lrom Cyprus. An aslerisk (') indieales new or devialing eounls, e indieales laxa endemie lo Cyprus. Taxon n 2n Fig. Coli no. Anacardiaceae Pistacia atlantica 15 Aparicio CY 10-2 Apiaceae Ainswor1hia trachycarpa 10 2 23-1059, Aparicio CY 5-2 Bupleurum gracile r 3 28-1308 Daucus guttatus 10 4 5-152 Daucus involucratus 11 5 13-490 Scaligeria napiformis 10 6 Aparicio CY 22-5 Tordylium syriacum 10 7 11-337 Zosima absinthiifolia 5' 8 15-614 Boraginaceae Onosma mitis 8' 9 26a-1194 Brassicaceae Alyssum cypricum 16' 10 Aparicio CY 31-1 eAlyssum troodi 8' 11 Aparicio CY 31-2 Arabis purpurea 16' 25 16-669 Biscutella didyma 16 Vogt 29-8808 Enar1hrocarpus arcuatus 20* 3-31 Matthiola longipetala subsp. bicornis r 12 13-497 Matthiola tricuspidata 16' 3-33 Moricandia spinosa subsp. maroccana 14 13 17-756 Sinapis alba 22 13-510 Torularia torulosa 7* 14 13-509 Turritis laxa 6' 15 21-834 C aryophyllaceae Cerastium comatum 17 16 21-814 Minuar1ia picta 11 ' 17 21-827 Petrorhagia dubia 30 26 10-306 Silene fruticosa 12 18 29-1377 Silene galataea 12' 26a-1202 • Silene gemmata 12' 19 34-1591 eS ilene laevigata 24' 34-1588 Silene macrodonta 20' 27 1-6 Silene nocturna varo brachypetala 24 35-1661 Silene rubella 24 4-90, 10-307 Stellaria ci/idea 13 ' 20 21-835 Helianthemum salicifolium 10 21 16-652 Helianthemum syriacum 10 22 Aparicio CY 15-1 Tuberaria guttata 18 23 440, 26a-1233 Tuberaria guttata 24 24 12-440
Boccon ea I I - 1999 119 Table 1. (continued). Taxon n 2n Fig. Coli no. Tuberaria p/antaginea 12 31 12-439, 26a-1232 Aparicio CY 23-4,24-1,24-2, 24-3, 24-4, 25 -1, 26-1, 28-1 Compositae Aetheorhiza bulbosa 18 28 Vogt 28-9044 eAnthemis tric%r 9* 13-505 Bellis annua subsp. minuta 90* 29 28-1326 Ca/endu/a arvensis 44 30 13-503, 16-653, 11347 Carduus argentatus subsp. apicu/aris 14 * 32 23-1061 Carduus pycnocepha/us subsp. 60 37 28-1333 a/bidus Centaurea aegia/ophi/a 11 Aparicio CY 2-4 e Cepha/orrhynchus cypricus 18* 38 21-847 Ch/amydophora tridentata 18* 39 5-127,8-276 Chrysanthemum coronarium varo 18 40 4-69 coronarium Chrysanthemum coronarium 9 33 13-465 Chrysanthemum coronarium var. 18 4-85 disc%r Chrysanthemum segetum ~ 18 41 Vogt 24-8641 Cnicus benidictus 22 42 30-1413 Crepis foetida subsp. foelida 10 43 Vogt 28-9040 Crepis fraasii 6 34 Aparicio CY 14-1 Crepis fraasii 12 44 26a-1193 Crepis sancta 10 45 6-207,34-1579 Crupina crupinastrum 28 46 6-212 Hedypnois rhagadi%ides 13 47 Vogt 28-9040bis He/ichrysum italicum 14 35 12-437 Leontodon tuberosus 8 48 23-1094 Matricaria reculita varo coronata 18' 49 6-189 Notobasis syriaca 34 50 4-82 Phagna/on rupestre subsp. graecum 9 36 8-278 Phagna/on rupestre subsp. graecum 18 51 6-185, 13-472 Phagna/on rupestre subsp. rupestre 18 52 3-30 Picris pauciflora 10 53 30-1412 Pseudognapha/ium /uteo-a/bum 28' 35-1663 Reichardia tingitana 16 64 28-1300 Scorzonera jacquiniana var o 14 ' 55 9-285 subintegra Scorzonera laciniata 14 56 8-275 Senecio /eucanthemifo/ius varo 20 57 34-1583 vernalis Senecio vu/garis 40 58 12-441 Sonchus oleraceus 32 59 26a-1209
1 20 Vog t & Aparici o: Chromosome numbers of plants c oll ec ted ... Ta ble 1. (continued). Taxon n 2n Fig . Coli no . Steptorhamphus tuberosus 16 60 26a-1211, 28 -1330 Tolpis barbata 9 61 29-1390 Tragopogon sinuatus 12 67 6-183, 12-424 Tyrimnus leucographus 17 62 23-1060 Urospermum picroides 10 68 6-203 Xeranthemum inapertum 14 63 12-426 Convolvulaceae Convolvulus oleifolius 15 * 64 5-175 Dipsacaceae Pterocephalus brevis 9* 65 31-1467 e Pterocephalus multiflorus 9* 66 12-436 Ericaceae Arbutus andrachne 13 73 Aparicio CY 17 -3 Fabaceae eAstragalus cyprius 8* 74 25-1140 Hedysarum spinosissimum 16 5-122 Hymenocarpos circinnatus 16 Vogt 14 -8359 Lotus peregrinus 28 31-1471 Medicago truncatula 14 31 -1468 Onobrychis crista-galli 32 4-46 e Onobrychis venosa 7* Aparicio CY 12-5 e Onobrychis venosa 14* 69 13-489 Ononis ornithopodioides 32 70 28-1292 Ononis viscosa subsp. sicula 32 71 31-1475 Scorpiurus sulcatus 28 72 8-277 Vicia sativa subsp. sativa 10 79 6-215 Geraniaceae Erodium cicutarium 20 * 80 6-217 Gramineae Avena ventricosa 14 15-600 Bromus caroli-henrici 14 ' 28 -1320 Bromus rubens 28 81 6-195 Iridaceae eGladiolus triphy/los 60* 82 Vogt 23-8806 Gynandriris sisyrinchium 24 83 Vogt 29-8809
Boccon ea I I - 1 99 9 12 1 Table 1. (continued). Taxon n 2n Fig. Coli no. Lamiaceae ePh/omis cypria var o occidentalis 10' 75 25-1143 Sa/via verbenaca c.42 13-478 Sa/via verbenaca 56 84 34-1577 Teucrium kotschyanum 32' 85 20-772 Liliaceae Allium junceum 8 76 23-1081 Allium trifoliatum 14' 86 24-1120 Bellevalia trifo/iata 8 87 11-358 Orchidaceae Ophrys transhyrcana 18' Aparicio CY 10-3 Orchis coriophora subsp. fragrans 18' 14-541 P/atanthera ch/orantha 21 77 22-966 Orobanchaceae Orobanche aegyptiaca 12 78 27-1288 Po/ygonaceae Emex spinosa 20 4-81 Rumex cyprius 18 88 31-1477 Primu/aceae e Cyc/amen cyprium 30 89 Vogt 21-8530 Cye/amen persicum 48 90 11-313 Scrophu/ariaceae Veronica persica 28 91 33-1523 Va/erianaceae e Kentranthus ca/citrapa subsp. 32' 20-779 orbicu/aris Va/erianella orientalis 16' 92 33-1522 Results The families, genera and speci es indi ca ted below are arranged alphabe ti ca ll y. F or a li speci es the name ado pt ed in th e preceding checkli sI of planls co llected during th e fo unh OPTIM A expedition (Alziar 1999 ) is gi ve n. Short co mment s are prov id ed for a li laxa studied in this in ves ti ga ti o n. The r es ults are s umm a ri zed in Table I.
1 22 A ll a c a rdia c ea e Pistacia atlantica Desf. Vogt & Apa ri cio: Chr omo some number s of plants co ll ec t ed .. . Il = 15. Limassol: Vouni , roadside , m ar ls a nel lime ston es, 480 m. 17 Ap r 1991 , (Aparicio CY /02) (F ig. I) . The ge nus Pist acia is known to h ave two different c hrom oso me numb ers with n = 12 a nd n = 15. In P. atlantica one deviating number , 211 = 28 is cited by Zohary (1 952) far P. lll/antica var. lat!lo/ia DC. Ap i acea e Ainsworthia trachycarpa Boiss. /1 = IO . Lìlrnaka: Ay ia Anna , dry sl opes , pal aeoge ne lim es tone a nd mari, c. 150 m, 15 Apr 1991 (Apar i cio CY 5-2). n = IO . Paphos: Between Nata and Axylon , ga rrig ue , limestone a nd marl-lilll es tone, 350 -400 m, 22 Apr 1 99 1 (23- /0 59) (F ig. 2). Severa 1 chromosome numb ers of Ihis E. Mediterran ea n t axo n have b ee n publish ed previou sly; n = 7 a nd Il = 9 are re ported by Co nstan ce & a l. (19 76 ) from I srae l anelli = IO from Lr aq, n = 8 and 2/1 = 16 respectively by AI-Ei saw i & Jury ( 19 88) and AI -Eisaw i ( 19 89) from JOI ·dan. Bupleurumgracile d' Urv. /1 = 7. Paphos: P ey ia, road to Polis, open Pi /1u S brutia for es t, lim es ton e, 250-300 m, 24 Apr 1991 (28- /30 8) (Fig. 3). This is the first report of a chrolllo so me numb er for this E. Medit elTa ne an spec i es . Dallcus gllttatlls Sm. /1 = IO. Larnaka: Cape Greco. lim es tone rocks (Iower Miocene ) and sand, 10-20 m, 12 Apr 199 1 (5-/52) (F ig. 4). According to pr ev ious studi es this spec ies see ms to be represented by two different c hrom oso me numb ers. Our co unt of /1 = IO is in acco rdan ce with the repart of 2/1 = 20 m ade by Cesc hm ed ji ev ( 1 983) who studi ed Bul garia n plant material. 211 = 22 chrol11osollles are cit ed by En gs tr and (19 70 ) and Il = Il by Co n sta n ce & a l. ( 1 976) a nel Striel & Franzén (1 98 1) re spec ti ve ly who st ucli e el plants of Greek pro ve nan ce. Daucus Ùll 'olllcraius Sm . /1 = Il. Larnaka: Ayia Anna, dr y slope s, palaeogene Iim es tone and marI. c. 1 50 m, 15 Ap r 1 99 1 (/3 -490) (F ig. 5) .
Bo cco nea I I - l!) !)!) 1 23 Our resull correspo nds with th e reporl of 2/1 = 22 for this taxon ciled 111 Engstrand (1970) for malerial from tlle Aegaea n r egio n. Scaligeria napiformis ( Spr eng.) Grande Il = IO. Paphos: Junction of tlle track lo Ayia (Panayi), va ll ey of Ezoussas, v ul ca ni c rocks, c. 450 m, 23 A pI' 1991 (Aparicio CY 22-5) (F ig. 6). With the co rr es ponding r es ull of 2n = 20 this speci es distribut ed in the eas tern Mediterran ea n r eg ion has previously been st udied by Engstrand (1970) using pIanI mat er ial of Greek provenance. Tordylium sy riacum L. n = IO. Limassol: Amathus, stony slopes, edge of ag ri cultural land, wa ll s, garr igue, ma ri and lim esto ne, 10-40 m, 14 Apr 1991 (J /-:f37) (Fig. 7). This is the first co unl based on authentic pIanI mate ri al of this sp ecies endem ie to Asia Minor. Previolls r epo rts of 2n = 20 and n = IO have been given by Gardé & Ma lh eirosGardé (1954), and Be li & Co nstance (1960) us in g plant material of unkn ow n origino Zosima absillthiifolia (Vent.) Link n = 5. Limasso l: Vouni, roadslde, limestone and ma ri , 480 m, 17 A pI' 19 91 (/5 -6 14 ) (F ig. 8). Zosima absinthiifo /i a is distributed in S. W. and C. Asi a. Two previous c hr omosome co unt s have been ca rri ed out on pl a nt mate ri al of Iranian pr ove nan ce yielding n = IO (Constance & a l. 1971 , 1976 ). According to the available data in c hrom osome in dices and karyological studi es dealing with Apiaceae (e.g. Delay 195 1, Cave 1959, 1960, 196 3, 1964, D ar lington & Wylie 1955, Ornduf f 1968, Fedorov 1969, Moore 1970, 1971, 1972, 1974, 1977, Beli & Co nstance 1957, 1960, 1966, Co nstance & al. 197 1 1976, Queir 6s 1978, Pastor 1993) the family is known to have basic c hrom osome number s from x = 6 to x = I I at least in th e Mediterran ea n area. Therefore our co unt of n = 5 for Z. absinthiifo li a is a n ew numb er for the sp ec i es as well as for th e whole family of Apiaceae. Bora g in aceae Ono s ma mitis Boiss. & Heldr. /I = 8. Paphos: JlIn ct ion of th e track to Ayia (Panayi), va ll ey of Ezo ll ssas, vo lcanic rocks, C. 450 m, 23 A pI' 1991 (26A- // 94) (Fig. 9). This is th e first karyolo gi ca l sllldy of this speci es which is r est ri cle cl lo Turkey and Cyp rus. The genlls 0l10.l'IIIa is known to represe nt basic c hr omosome number s from .r = 6 IO X = Il (for r efe rence see Luqlle 1990). /I = 8 has b ee n r epo rt ed previously o nl y for O. se ri ce um from Arme ni a (Tissot-Dag ll ette 1972).
124 Vo gt & Aparici o: Chromosome num be rs of plants coll ec t ed ... ' . . 04' . , Figs. 1-6. PMC Ill eiosis o f: I, Pi s/ac ia alla n/i ca, Il = 15 ; 2, Ail1swo nhi a /rach yca rpa. 11 = I O; 3, Bupl eu rul1l gracile. n = 7: 4, D au cu.\· gU lla lUs. 11 = IO ; 5, Dau cus involucra/us. Il = Il ; 6, Scali ger ia l1 apiformis. 11 = IO . - Scale bar = IO plll .
Bocconea \ \ - \999 \ 25 "' .; .. · 1"1'· . , 7 { 8 r\ Fi gs. 7-12. PMC Ill t: i os is or: 7, ·/ im /vliu/II sy ria clIIlI. /I = I O: S, /. OS illl ll IIl!si lllliii/ à/i ll. /I = ."i: I), Oll os/lla /IIi/i s. Il = 8; IO , iI /y ss u/II cy pri clIIlI. /I = 16: Il , iI/yssu/II /rom/i. Il = 8; 12, Ma llhio/ II /ollg ip e /a /a subsp. bi co mi s. Il = 7. - Sca le bar = IO p Jl1 .
1 32 Vogt & Aparicio: Chr olllosome nUlllbers or plants co ll ected ... C i s/ocea e FUII/allll arabi ca (L.) Spac h /1 = 1 6. Larnaka: Stavrovouni , reforestation ar ea, diabase , basalt and gabb r o, 450ROO 111 , 15 Ap r 199 1 (/2-4/8) . Qur r es ult co in cid es with the co unts or 2 /1 = 32 report ed by Chiarugi (1925) from It aly. Atslllon & Feinbrun (1960) from Israel, and Markova (1972. 1975) rrom Bul garia . He[iallthemllll/ salicifo[illll/ (L.) Mili. /'I = lO. Limasso l: Ayia Mavri, Kilani, stony sl opes , ma rl s and lim esto ne, 700 m, 17 ApI' 199 1 (/6 -652) (F ig. 21 ). Qur co unt corrobo rates the reports ( Il = IO or 2/1 = 20) by many authors throughout the M ed iterran ea n distribution range. Material of Cy prian provenan ce has alr eady been studi ed and the sa me r es ult was publish ed by Slavik & a l. (1993). He[ianthell/Ilm syriaclll1l (Jacq.) Dum . Cours . (= H. laval7dulifoliul1I auct.) 17 = IO. Larnaka: Betw een Vavla and Kirokitia, lim es tones, 520 m, 18 Ap I' 1991 (Aparicio CY /5-1) (F ig. 22). Prcvious co unt s (11 = IO and 2/1 = 20) for this Mediterrane an taxon reported by Co utinho & Lorenzo-Andreu ( 194 8) . Valdés & al. ( 19 78), Valdés - Bermejo ( 19 80) from Spain and Atsmon & Feinbrun (1960) from lsrael yie ld ed the same result. Tuberaria ( Dun al) Spach As karyolo gical investigations in S. W. Spa nish population s or different species of Tuberaria h ave sh ow n (Ga ll ego & Aparic io 1993) this ge nus is t axo n omica ll y very co mpli cated due to hybridi zat ion a ncl b ac kcr oss ing amo ng th e taxa. A co nsi de rable morphol og i ca l va ri at ion is l eading to enormous difficulties in species de limit a ti o n. QUI' karyological stu dy of Cyprian material gives raise lo the assumpt ion that h ybridiza ti on and polyploidy al so occ ur s in this pa rt of the distJ"ibution range. T. plal7/aginea r ep r ese nts the t et raploid l eve I (2 17 = 24) and T. gulfata th e h exap loid l eve l (217 = 36), wi th some , probably a ll o-octop l oid, hybrid forms (217 = 48). Tllberaria gllttata (L.) Fourr. 17 = I R. Paph os: Junction of the track to Ayia (Pa nayi). va ll ey of Ezo ussas, vo l canic rocks, c. 450 111 , 23 Ap I' 199 1 (26A- /233) (Fig. 23). Il = 24. Larnaka: Stavrovo uni, r efo r esta ti on ar ea, diabase. basalt a nel gabbro. 450-800 111 , 15 ApI' 199 1 (/2-440) (F ig. 24). The polymorphic Tuberaria guttata is a species widespread in the W. Mediterranean but rare in the eas tern p cu · t. It has been studie cl kar yo l ogica ll y in it s whole distribution ran ge al ways show in g th e SLl llle ploidy l eve ls (fo r previous ch romosome coun ts see Gallego & Aparicio 1993).
Boccon",,, I I - 1 999 133 Tuberaria plallfaginea (W ilkl.) Gallego Il = 12. Lamaka: Stavrovouni. r eforestation area , diabase , ba sai t and gabbro. 450 - 800 111 , 15 Apr 1 99 1 (12-439). Il = 12. Papho s: Jun ction of the track to Ayia (Pa nayi), Valley of Ezo u ssas . vo l ca nic ro cks , c. 450 111 , 23 Apr 1991 (26A-1232). Il = 12. Paphos: Peyia , road to Polis, open Pinu.\" brutia forest, lim esto ne, 250 - 300 111. 24 Ap r 19 9 1 (Aparicio CY 234) . Il = 12. Paphos: Near Dhroushia and Inia, west of mount Lara , meadows and big rocks of triasic sa nd ston es. 550 111 , 24 Apr 1 99 1 (Aparicio CY 24-1 ,24 -2. 24-3,24-4). n = 12. Nikosia: Ay ia lrini , edge of ag ri c ultur al l and , gab bro and diorite, 950 m, 26 Ap r 1991 (Aparicio CY 25-1). n = 12 . Nikosia: N. E. of Ayios Yeoryios K avka lou (road to Vyzakia) , roads i de a nel ag ri c ultur al la nd , sand and rubble (b as alt), 390 m, 26 Ap r 1991 (Aparicio CY 26 -/ ). /I = 12. Nikosia: Alona, steep sl opes, garrigue , di abase , 940 111 , 26 Apr 1991 (Aparicio CY 28- /) (F ig. 31). Co mp os it ae Aetheorhiza bulbosa (L.) Casso 2n = 18. Paphos: Peyia , road to Po li s, open Pi/lus brutia foresl, lim estone , 250 -3 00 m. 24 Apr 1991 (Vogt 28-9044) (Fig. 28). Our co unl of 2/7 = I R agrees with former reports given by Pavone & a l. ( 1 98 1 b) for lt aly, Norelen stam (1972) for Egyp t, Dahlgren & a l. (1971 ) for the Balearic Islanels. Delay & Petit ( 1972 , sub Crepis bulbosa L.) for Fra n ce, Fernandes & Qlleir6s ( 1 97 1 a) for Portu ga l, a nd Castroviejo ( 19 83) for Spain. Mori (1957) fo und a different numb er of 2 11 = 32 in plant mat er ial of Italian provenanee. Anthemis tricolor Boiss. n = 9. Larnaka: Ayia Anna. dry slopes , pal aeoge ne lim esto ne and mari, C. 1 50 m. 15 Apr 1991 (/3-505). This is the first r eport of a chromosome number of this s peeie s endem ie to Cy prus . BeLlis annua subsp. minuta (DC.) Meikle 2 11 = 90 . Papho s: Peyia , r oad to Poi is, open Pi/lu.\" brutia forest, I imestone , 250300 m. 24 Ap r 1 99 1 (28 - /326) (F ig. 29 ). Bellis an //ll a L. sllbsp. lIIi/luta h as not been s tudied cyt.ol og i ca ll y befor e anel the deeaploid numb er or 211 = 90 h as been fO llnd for the first time in the gen us Bellis. Previolls cou nts in B. an//lla reslIll ed exclusive ly in the diploid number 2/1 = 18 or /I = 9 r espec ti ve ly and were reportecl by RlIiz de Clav ij o Jiménez (1 9RR) from Spain. Pa vo ne & a l. (1 98 1 a) l' rom Italy, and Fernandes & Qlleir6s (1 97 1 a, 19 71 b) l'rom Portllgal.
134 Yog t & Aparicio: Chromosome numbers of pla nt s co ll ected ... l'igs. 25 -30. ML 'lap l1 ascs or rool-lip llliI OSl!~ o r: 25, J\mhis / JII I,/ }f/J'('lI. 2 11 = 16. 26, /' l' lro rr/ /(/ g ill du bia. 2 11 = 3 0; 27, Sil e ll e II lC1crodo lll Cl . 2 /1 = 20; 28, J\e lh eor hi za /) //I /)osCi . 2 11 = 1 X: 29, lJ e lli s lI/I/I//a subsp. /IIi l1l ll a. 2n = 90; 30, Calendu la a rl 'e /l si s. 2 /1 = 44. - Sca le bar = IO p m.
B occo nea 1 1 - 1999 1 35 l, i )!~ . . \ 1-,\( 1. 1'1 \'ll ' 1l1 <: i ll~ i , il i' j I, /II I)( ,/, (// III /' / fl/I/ I1 !, /I /{' u, l/ = 1 2: .H , ('(/ rd/l/ /.\ arge l/l lIlIl.\ ' 1I 11 , p. a pi c ul a ri s, l/ = 14 110 11 + () ~ ): 33, Chr vs anthl' //I/./I/I c oron a ril l lll val'. corUl/a ri u l/l , 1/ = <) ; 34, Cre/ li .l' .fi'aas ii , 1/ = 6; 35, Il e !i c hr Y, l'f. ulI iW!i O UII , 1/ = 14; 36 , Phagnalon rt/ pes lr e subsp, grac cllIIl, Il = 9. - Scale ba r = I O ~11l ,
136 Vogt & Aparicio: Chromosome numbers of plants co llectecl ... Calelldula arvens;s L. 2/1 = 44. Larnaka: Ayia Anna, dry slopes, palaeogene limestone ancl mari, c. 150 m, 15 Apr 1991 (/3-503). 2/1 = 44. Limassol: Amathus, stony slopes, eclge of agricultural land, walls, garrigue, ma ri and limeslone, 1 0-40 m, 14 Apr 1991 (1/-34 7). 2/1 = 44. Limassol: Ayia Mavri, Kilani, stony slopes, marls ancl limestone, 17 Apr 1991 (/6-653) (Fig. 30). Material from three Cyprian populations of this widespread Mediterranean annual proved to have chromosome number s of 2/1 = 44. This result corroborates reports from numerous countries cited in Oberprieler & Vogt (1993). Deviating counts made by Humphrie s & al. (1978, 2n = 18 ) and Borgen (1974,2/1 = 42) are attributed to misidentification or miscounts respectively (see Oberprieler & Vogt 1993 for discussion). Carduus argentatus subsp. apicuLaris (Bertol.) Meikle n = 14 (1011+ 08). Paphos: Between Nata and Axylon, gan'igue, limestone and marllimestone, 350-400 m, 22 ApI' 1991 (23-/ 06/) (Fig. 32). This is the first publication of a chromosome number for this E. Mediterranean species. The chromosomes during diakinesis and metapha se show ten bivalents as well as a ring of eight chromosomes, probably due to the existence of translocation heterozygosity. As far as we know this is the first ti me that this kind of chromosome rearrangements has been found in Carduus. Carduus pycnocephallls subsp. albidus (M. Bieb.) Kazmi 211 = 60. Paphos: Peyia, road to Polis, open Pil1us brutia fores t, limestone, 250-300 m, 24 ApI' 1991 (28-/333 )(Fig. 37). Our count of 211 = 60 agrees with reports from many countries cited in Oberprieler & Vogt (1 993) and from Palestine (Diaz Lifante & al. 1992). A comprehensive discussion of numerous deviating counts is given in Oberprieler & Vogt (1993). Centaurea aegiaLophila Wagenitz (Aegialophila eretica Boiss. & Heldr.) /1 = II. Larnaka: Cape Greco, limest one rocks (Iower Miocene) and sand, 10 -20 m, 12 Apr 1991 (Aparicio CY 2 -4). Our co unt is in accordance with tbe diploid number 2/1 = 22 reported by Runemark (1967) who studied plants from Crete. Cephalorrhynchus cypricus (Beauvercl) Rech. f. 2/1 = 18 . Paphos: Mount Tripylos and Cedar valley, forest with Cedru.I' brevU'olia a ncl
[3o cco nea I I - 1999 1 37 Quercu.\ aln(f'olin. 1080-1360111 , 20 ApI' 1991 (2/-847) (Fig . 38). Our counl is ùl e firsl re porI for this endemie Cyprian species growing on shady mounlainside s or str ea msides al high altitudes of Ihe Troodos Range. Chlamydophora tridentata (Delile) Ehrenb. ex Les s. 21'1 = 18. Larnaka: Between Men eo u and Men eo u beach, 5-10 m, 13 Apr 1991 (8-276). 2/1 = 18 . Larnaka: Cape Greco , limestone rocks (Iower Miocene) and sa nd. 10-20 m, 12 ApI' 199.1 (5-/27) (F ig. 39). Material from two Cyprian population s of this ephemeral species proved to have chromosome numbers of 2/1 = 18. There are no previous indi ca tions for the monotypic genus Chlamydophora distributed from North Africa ( Tuni sia and Egypt) to Crete and Cyprus. Chrysanthemum coronarillm varo coronarillm 2n = 18 . Larnaka: Between Xylophagou and Ayia Thekla , fallow land and pseudosteppe with Sarcopoferiu/1/. C. 5 m, 12 ApI' 1991 (4-69) (Fig. 40). /1 = 9. Larnaka: Ayia Anna, dry slopes, limestone and ma ri , ca . 150 m, 15 Apr 1991 (/3-465) (Fig. 33). Chrysanthemllm coronarium L. val'. discolor d'Urv . 2/1 = 18. Larnaka: Between Xylophagou and Ayia The kla , fallow land and pseudosteppe wiÙ1 Sarcopoferiu/1/. C. 5 m, 12 ApI' 1991 (4-85) . Our counts of three Cyprian populations corroborate Ù1e numerous former reports for Ù1e two varieties of this common Mediterranean weed from OÙler countries ali having 2/1 = 18 or 11 = 9 respectively and being cited in Oberprieler & Vogt ( 1993 ). Chrysanthemllm segetllm L. 2/1 = 18. Paphos: Between Eledhiou and Amargeti, gypsum, 360 m, 22 ApI' 1991 (Vogt 24 - 864/ ) (Fig. 4 1). This first reporl of a chromosome number for Cyprian plant material of this species corroborates previous counts in the Mediterr a nean area cited in Vogt & Oberprieler ( 19( 3). Cniclls benedictlls L. 211 = 22. Nikosia: Ayia Irini, edge of ag ri cultural land, gab bro a nd diorite, 950 m, 26 ApI' 1 991 (30-/4/3) (Fig. 42). Our co unt of 2/1 = 22 chromosomes is a co nfirmation of former repo rt s for th e Mediterran ea n area made e.g. by Kuz manov & Nikolo va ( 19 80), Ubera (1979), FerlHlnelez Casas (1977) anel Fernancles & Queir os ( 1971 a). This speci es is widely distribute el in the Meelilerranean r eg ion anel eastwarels to Iran anel Afghanistan.
Vo gt & Apa ri cio: C hromo some numb ers of pl a nt s co ll ected ... . ' . , .•. .. ~. ~~~, . .. ":'. ~ . ~/ " ~}:~ .' . ; " ,~ .. .. ~ '., .- f ' . .. " .. ~ ': ., .. :, ~ ~ ." ,.. ~ .. •• • l . :, .; : : :. {'~ .;)' , . " ... .. Fi gs. 37-42. Ml'lapli;l >l's 01 l'Onl-lip mil oses or: 37, Cu rduu s /' YC II OU ·/ ,!t"/If .1 > 11" >1' . II//J/If l/.l . 2 11 = (,(): 3H, Cl' p/ llI lorhvnc hu s cV l'ri cus. 2 /1 = I X; .W, ChlulI/ ydop ho/'{/ fr id cl1lu/a. 2 11 = I X; 40, C hr ysu/lfhl'l'llul1I coro nariwl/ val'. coronuriul1I, 2 11 = I X; 41, C hr y SlI l1f hell/U/1/ sl'gl' fu l1I . 211 = I X: 42, Cnicus bCl1cdicfus. 2 /1 = 22 . - Sca le bar = IO 11m .
Bocconea 1 1 - 1 99 9 139 Fi gs. 43-4~ : Metap ha ses of roolli p IIlil OSeS of: 43, Crepisfo(' li da subsp.jòelid a. 1 1/ = IO : 44 , Crepi.l· .fi"a as ii. 2 1/ = 1 2: 45, Crepi.l· sO l/ cIO. 2 1/ = I O: 46, C mpi l/a c rUp i l/ aslmlll. 2 /1 = 2H ; 47, lI edYP l1 0is rh agadioloid es. 2 /1 = 13; 48, Leo l// odO I/ lu uerosus. 2 /1 = 8. - Sca le ba r = I () pl11.
140 Vogt & Apa ri cio: Ch romoso me nUlllbers of plants co ll ected ... Crepisfoetida L. subsp. foetida 2n = IO. Paphos: Peyia, road to Po li s, open Pinus brutia for es t, limestone, 250-300 111 , 24 ApI' 1991 (Vogf 28-9040) (F ig. 43). Crepis foetida is represented in the fl ora of Cyp rus by subsp. fo etida am i subsp. commutata. Our co unt of 211 = IO in a population of subsp ./o etida agr ees with a li previous reports for this taxon (e.g. Mejias 1 986 and Luque & Diaz Lifa nt e 1 99 1 for Spa in ; D'Ovidio 1986 and Bartolo & a l. 1978 for Italy; Loon & Ki eft 1 980 and Gadella & Kliphuis 1 972 for Ju gos lavia; Fernanues & Queiros 1 97 1 a for Portugal; Ku zma nov & Nikolova 1 980 and Kuzmanov & K oz uh arov 1970 for Bulgaria; Strid & Franzén 198 1 for Greece ; Kliphuis & Wieffering 1972 for France; Miijovsky & a l. I 9 70a for Sl ovak ia). Crepisfraasii Schultz-Bip. /1. = 6. Larnaka: Road from Lefkara to Vavatsina, 4-5 km from Vavats in a, volca ni c rocks, 750 m, 18 Apr 1 99 1 (Aparicio CY /4-1 ) (Fig. 34). 2n = 1 2. Paphos: Junction of the track lo Ayia (Pa n ay i), Va llay of Ezo ll ssas, vol ca nic rocks, c. 450 m, 23 Apr 1 99 1 (26A- // 93) (Fig. 44). This E. M ed iterranean speci es has formerly been studi ed in Greece and Crete by Striu (1983), Strid & Andersson (1 985), and Anagnostopollios & K ama ri (1992) with th e co rr es ponding r es ull of 2n = 12 . Crepis san.cta (L.) Bornm. 2n = IO. Larnaka: salt lake n ea r Larnaka, 1-2 m, 13 Apr 1 99 1 (6-207). 211 = IO. Limassol: between Mandria and Ayios Nikolaos, roadsides and garrigue, gabbro and limestone, 27 Apr 1 99 1 (34-/ 579) (Fig. 45). Our co unt of 2n = IO in plants of two Cypr ian populations co nfirm former r epo rts from Iran (Ghaffari & Chariat-Panahi 1 985) , Bulgaria (Kuzmanov & al. 1981, Ku zmanov & Juruk ova 19 77 ), France (Nata rajan 1978), and Greece (Str id & Franzén 198 1, Kama ri & Anagnoslopoulos 1991 ). Crupin.a crupinastrum (Mo ri s) Viso 2 /1 = 28. Larnaka: Salt lake n ea r Larnaka, 12 m, 13 Ap r 1 99 1 (6-2 / 2) (Fig. 46). Our r epo rt of a c hr omosome nllmber f or this wides pr ead mediterranean species is in acco rcl ance with th e former reports of Slavik & a l. (1 993) for Cyp rus, KlIzmanov & a l. (1 986) for Bulgaria, Loon (1980) anù Strid & Franzén ( 1981) for Greece, P avo ne & a l. (198 Ib ). Scr ug li & Bocchieri (1976), Larsen (1956a), and Co lombo & a l. (1 979) for It aly, COlluerc ( 197 9) for France, Kliphuis & Barkouuah (19 77 ) for Syria, Da hl gren & a l. (19 71) for the Balearic Isla nd s, Luque & Diaz Lifante (1991), a nd Ru iz de Clav ij o J im én ez (1990) for Spain.
13o ccon ea I I - 1 9CJ9 141 • • Figs. 49-54. MClaphasl's or roOl-l ip mil oses o r: 49, Molr;mr; a r eC f.lI;1O va l' . co ro//olO . 2 // = I X: 50, NOIO/ms;s sy r; oC({. 2 // = . \4 ; 51, J>/ wg /1 a/o// n/p c. "/"( ' slIbsp. gran l//II. 2/1 = I X: 52, / 'ho g // a/o // rup es lr !' s lIb sp. n/IJ('sIN ·. 2 // = I X: 53, /' ;l'r;.I' IJIII/ eU/ora , 2 // = IO: 54, Ne;eha rd;l/ 1;//g;IO// O. 2 // = 16. - Sc ale bar = IO pm .
14R Vogt & !\ paricio: Chr omosoll1e nllll1b ers of plants co ll ec led .. . No former r epo rl of a ehrolll oso me nllmber for this ende mi e Cyprian speci es is known. Erica ce a e Arbutlls andraclllle L. n = 13. Papho s: MOllnt Tr ipylos and Cedar valley, forest with Cedrus brevi/olia and Quercus a lni/ oli a. 10 80-1360111, 20 Apr 1991 (Aparicio CY 17-3) (Fig. 73). Ollr sf lldy confirm s th e diploid number 2/1 = 26 forme rl y reported by several aUlhors rsee Darlington & Wylie (1955: 2 16 ) and Fedorov (1969: 265)1 for this E. Mediterran ea n spec ies. Faba ceae Astragallis cyprius Boiss. 11 = 8. Paphos: Pano Panayia, lim es tones, ga rrigue and m ea dow s, 820 m, 22 Apr 1991 (251/40) (Fig. 74). There are no former report s of a c hromo so me numb er for Ihis endemie Cypr ian sp ec les. Hedysarlll1l Spillosissimllm L. 2n = 16. Larnaka: Cape Greco , IIm es t one rocks (low er Mio ce n e) and sand, 10-20 m, 12 ApI' 19 9 1 (5-/22). Our result eonfir111s previous re port s from Cyprus (Slavik & al. 1993), Malta (Kramer & al. 1972), Is ra el (Heyn 1 964), lt aly (Brullo & al. 1979), Spain (Dahlgren & al. 1971), and Algeria (Abde lguerfi-Berrekia & a l. 1 986). Hymellocarpos circinllatlls (L.) Savi 2n = 16 . Limassol: Betwe en Kambou and Souni, ga rrigue, lim es tone and mari, 280 m, 17 Apr 1991 (Vogt 14-8359). Our co unt of 2/1. = 16 is in accordance with reports given by Colombo & al. (1979) for Italy, Cartie r (1 976) for Syria , AI-Mayah & AI-Sh ehbaz (1977), and Diaz Lifante & al. (1992) for lsrael. Lotus peregrinus L. 2/1. = 28. Nikosia: N. E. of Ayio s Y eo ryios Kavkalou (road lo Vyzakia) , roadside and agriculturallancl, s and and rubble (basalI), 390 m, 26 Apr 1 99 1 (3/ - /4 71). Thi annual E. Mediterranean species of Lotus seet. Xa/1.tholoru s was formerly studied with the same result by Bartolo & a l. (1979) using Ttali an, and Larsen (1956b) us in g Palestinian pianI material.
Bo ccone" 1 1 - 1999 . , .j • ( 149 " .' h~,. 6 1-66. PMC Ill (' i os is or: 61, To lpi s !JlIr/)rt/tI. Il = I): 62. l l'rt l//IlII .\ Ic/w(} .!: r'//'/II/ .,. Il = 1 7: (,J, Xe rt/ //I!tc/lII//7/ il/lIp e r/l/Il/ . Il = 14; 64, CO III'o lmlll .\ olc(f() !ill s. /I = 15 : 65, l '/er(}('('p i/(/III.\ /;1'('\';.1' . Il = 9: 66, Pleroce ph all/s /I/ull!{lort/s. Il = I) . - Sca le bar = IO lllll.
1 50 V og t & Aparicio: Ch romosome nUlllbers of plants co ll ec teu ... Figs. 67-72. M e l a pila s~s of rool-lip mi loses o f: (17, "l"ra go Jl ogo// s i//l/fIIUS. 2 //= 12: 6K , lJmsJleolll l// 1 Jli croides. 2 // = I O; 69, U// o/; r yc his \·e // osa. 2 // = 1 4; 70, U/1o/1i s ornitho !, odioides. 2 /1 = 32; 71 , Orumis \'iscosa subsp. sicula. 2 /1 = 32; 72, Scorpiurus su/ca lU s. 2 /1 = 2X. -Scale bar = IO pm .
B occo n ea 1 1 - 1999 151 Medicago truncatula Gaertn . 2 11 = 14 . Nikosia: N. E. of Ay i os Y eo ryios Ka vka lou (road to V yza ki a), roadside and agricultural land, sa nd and rubble (basa lt ), 390 m, 26 ApI' 1991 (3 /-/4 68). Our co unt of 2 /7 = 14 is in accordance with Humphri es & a l. (1978) but differs from the repo rt s of 211 = 16 by Aga r wa l & Gupta (1983). Abdelguerfi & Guittonneau ( 1980), J oo n & Jong (1978), Fernande s & a l. ( 1977), Cl emen t (1962), Dahlgren & a l. (1971), and Simon & Simon (1965). Onobrychis crista-galli (L.) La m. 2/7 = 32. Larnaka: Between Xylophagou and Ay ia Th ekla. fallow land and pseud os teppe with Sarcopoteri ul1I , c. 5 m, 12 A pI' 199 1 (4-46). This co unt of a letraploid chrom osome number for O/l ob rychis crista-galli co nfirms the co unt given by Ob erpri eler & Vogt (1994). The sp ec i es has b ee n s tudied pr ev iously in Usbe ki stan (Ba ykab il ov 1977), Iraq (A IMayah & AI-Sheh baz 1(77 ), Syria ( Kliphui s & Barkoudah 1977), Israel (H ey n 1962), and Morocco ( Ob erpriel er & Vogt 19 96) with the l' es uli of 2/7 = 16 . Fo ll ow ing H ey n (1962), th e tetraploid numb er of 2/7 = 32 is m ore likely lo indicate th e pr ese n ce of th e c1 0se ly related tetr ap loid O. squarrosa Vivo On tbe island th an the occ urr en ce of a seco nd ploidy l eve l witJ lin O. crista-galli. Onobrychis ve nosa (Desf.) Desv . 2 11 = 1 4. Larnaka: Ayia Anna. dry slop es , palaeogene lim esto ne and mari, C. 1 50 111 , 15 A pr 1 99 1 (/ 3-489) (F ig. 69). 11 = 7. Limasso l: Between Ka mb ou and So uni, ga rrigu e, lim esto ne and ma ri , 280 m, 17 ApI' 199 1 (AparicioCY /25) . The se seem to be th e first co unts for this endemie Cy prian speci es . Onol/is omithopodioides L. 2 11 = 32. Paphos: Peyia , road to Po li s, open Pillus brutia foresto lim es ton e, 250-300 m, 24 A pI' 19 91 (28-/ 292) (F ig. 70 ). Our co unt of 2 /1 = 32 is in acco rdan ce wi th th e rep orts l11ade by Co l ombo & a l. (1980) and Sc rug li & B occ hi e ri ( 1977) from Italy. and San udo & al. ( 1 976 & 1979) from Spain. Bartolo & a l. (1978) give a de viating co unt of 2/1 = 20 ch romoso m es fOllnd in plants or Italian or ig in o O/lOl/is viscosa subsp. siC ilIa ( GLI SS.) Hub.-M or. 2 /1 = 32. Nikosia: N.E. or Ayios Y eo ryios K avkalou (road to Vyzak ia). roadside and agricultllral land, sand and rllbble (basa i t ). 390 111 . 26 Apr 1 99 1 (3/ -/475) (F ig. 71 ).
1 52 Vogt & Apa ri ci o: Chrom oso me numbers of pl a nt s co ll ec te cl ... l'hi s taxon has b ee n th e subject of cytol og i ca l in ves ti ga ti ons only twice before. Our co unt of 2 /1 = 32 co nfirms th e co unts given by Sa lÌucl o & a l. (19 76 & 197 9. sub O/loni .\" sic ul a Gu ss.), who s tucli e cl pl ant mate ri al of Sp anish o ri gino Scorpiurus sulcatlls L. 2n = 28. Larnaka: Belween Men eo u an cl Men eo u b eac h, 5-10 m, 13 Apr 19 91 (8 - 277) (F ig. 72 ). l'h e r es ult of 2/1 = 28 c hr om oso m es is in acco r cla nce with a li fo rm er reports for Ihis wicl es pread sp ec i es cited in Ob erprieler & V og t (1 99 6). Vicia satilla L. s ub sp saliva 2n = IO . Larnaka: Sa lt lake n ea r Larnaka, 1 -2 m, 13 Apr 1991 (6-215) (Fig. 79). For Vicia saliva c hrom oso me numb ers of 2/1 = IO , 12, 14, 24 and 48 are kn ow n, 2 /1 = 12 being tJle m os l frequent one. Ge r a nia ce a e Erodillm ciclllar;llm ( L. ) L'Hé r. 2 /1 = 20. Larnaka: Sa lt lake n ear La rn aka , 1-2 m, 13 Ap r 1991 (6-217) (Fig. 80 ). Erodium cicufarium, wid es pread in Europe, th e M ed iterran ea n. As ia and as an a li en in man y other r eg ions, norma ll y is a tetra pl o id with 2 /1 = 40 (Guittonn ea u 1972 ). Our Cy prian planls, det ermined by Prof . Guittonn ea u (Orl éa ns), proved to be diplo icl with 2 /1 = 2 0. a chrom oso me numb er previously reporteò by Alves & Leitao (1976) for E. cicutarium subsp. j ac quinianu lll (Fisch er & a l. ) Brig. from Portu ga l, Guittonn ea u (1972) for s ub sp. on tigola num Guittonn ea u from Sp ain, D evesa ( 19 81) for s ub sp. cicutarium from Sp a in , and Lave & L ave (1 94 4), Warb ur g (1 938), and Ro tt ga rdt (1 956) without indi ca ti on of a infrasp ec ific t axo n. G rami nea e A l'ella velltricosa Balansa ex Co sso 2n = 14. Lima ssol: Vo uni. road si de, lim es tone an cl ma rI , 480 m, 17 Apr 1 99 1 (/5 -600). l'hi s sp ec i es has b ee n stuòied o nl y twi ce before by Ladiz in sky & Zo hari ( 1 967 , 19 71 ). l' he diploid c hr om oso me nUlllber of 2 /1 = 14 is co nfirm eò here. Bromus caroli-hellrici Gre ut er 2 /1 = 14. Paphos: Peyia, road lo Po li s. ope n Pi/llls bra /i li fo r es t, lim es lone. 250 -30 0 n 1. 24 Apr 1 99 1 (28-1320).
Bo cco n ea I I - I <)<)9 1 5~ This is th e first report of a chro l11 oso mc numb er of this species which is c10sely relat ed to Brol1lus alopecur o.\' Poir. It s distribu ti on ran ges in the eas tern Mediterranean from Crete and the so uthern Aegean islands to S. Turk ey, Sy ria and Palestina. Bromus ruben s L. 211 = 28. Larnaka: Sali lake near Larnaka. 1-2 111 , 13 Apr 1 <)9 1 (6-195) (F ig. 8 1). Qur cOl mt of 2/1 = 28 co nfirms th e tetraploid nature of this species and is in acco rdan ce with a li previolls r epo rts from many co untri es l11ade e.g. by Humphri es & a l. (I <)78. Al ge ri a), R eese (1 95 7, Al ge ri a), Bjorkqvist & a l. (1 969, Spain ), Go uld (1 970) , QvadiahuYavin (1 969, Palaestina), Dahlgren & a l. (1971 , Balearic Islands). Natarajan (1 979 , Fra n ce), Kliphuis & Wieffering (1 979 , Aserbaielshan), anel Sanc h ez Anta & a l. (1 986. 1 988 , Spain ). Iri daceae Gladio/Ils triplzyllos (Sm.) Ker -Gaw l. 2n = 60. Paphos: Between Nata anel Axyl on , ga rrigue, lim es tone and marl-lim es tone. 350-400 m, 22 Apr 1991 (Vogf 23-8575). 2/1 = 60. Papho s: Near Dhrous hi a and Inia. W. of mount Lara , meaelows a nel bi g rocks of triasic sa nel stone, 550 111 ,24 Apr 1 99 1 (Vogf 8806) (F ig. 82). Th e cy tol ogy oft hi s s pecles has not b ee n stu dled previou sly. Gynandriri s sisyrinchium (L.) P ar I. 2/1 = 24. Paphos: Near Dhrou shia a nel Inia, W. of 111 0 unt Lara, meaelows a nel big r oc ks of triasic sanelstone, 550 m, 24 Apr 1 <)9 1 (Vogf 29-8809) (F ig. 83) . This is the first chr omosome co unt us in g Cyp ri an plant material of this wid esp reael Meeliterranean specie s. Qur r es ull is in acco rdan ce wit h former r eports (m os tly cited under Iri s sisyri/1chiu l1l L.) by Garbari & C ri sman (1 988), Qu eir os (I <) 80), Go l db latt (19 71 , 1 980) , Ri cc i ( 197 0-7 1), Ruiz R ej6 n (1 974), Delay & Petit ( 1 97 1), Fernandes & QlIeir 6s (19 71 b), and Fernan dez Morale s ( 1 974). A ele viating ehromosome numb er of 2n = 4X is give n by Montmollin (1 986) who s tlleli e el plant mat erial of Cretan provenance. I, a /11 i a c e a e Pltlomis cypria var. occidentali .\' Meikle Il = IO . Paphos: Pano Panayia. lim cs ton e, ga rrig ll e and mead ows. 82 0 111. 22 Apr 1 99 1 (25 -//4 3) (F ig. 75 ). Th ere are no pr ev io ll s chro l11 oso me nUlllber r epo rt s for this taxon endemie to th e fl ora 01' Cy prlls.
154 • • • • , .- # . ,,~ ~ . ,. " . 77 ;" i' Vog l & Apa ri cio: Chrolll oso me Ilumbers 0 1' plalllS co ll ec led ... . , . ... • • • - r ~. Figs. 7.3-7'1\ . l' MC Ill eiosis of : 73, II rbl//us a l/llra c hll e. ti = 13: 74, IIs / ra g (/ l us cv pr ius. Il = X: 75, Plil omi.l· cy pri u var. oc ci de ll ta li s. Il = IO ; 76, II lI iuIII JU II CC I/III. Il = X; 77, Pl a/u lI/l wl"C/ c h/ ora ll/h a. Il = 21; 78, Orobo ll che oegyp/iaca. Il = 1 2. -Scale bar = IO P 111 .
Bocconea I I - 1<) <)<) ... . • . " " ·1 ft :, . ~ . . "" "''''' . . , " .. ,. , -~ , ,~ " 1 55 Figs. 7~H54 . M t.' taphases of root-tip Illit oses of : 79, Vicia sa/im subs p. s o/im o 2 /1 ; IO ; SII, I:'rodilllll ci ClilariulII. 2/1 = 20; SI , llrolllus rub c ns . 2 /1 = 2X; S2, (;t ad io/u.\· /riphvl/os. 2 /1 = 60; S3, (;v/1o/ldriris sisy rill chil/ll/. 211 = 24; 84, Sa /I 'ia \'cr/Je//ll c(/ . 2 //; 56. -Scale bar = IO 11m .
1.'16 Vog t & Apa ri cio: Chro lll oso me nlllll bers or plants co ll ec ted . .. Salvia verbellaca L. 2/1 = 42. Larnaka: Ayia Anna, dr y slop es. pala eoge ne lim cs t one an tl mari, c. I SO m. 1.'1 Ap r 1 99 1 (/ 3-470). 2/1 = 56 . Limass ol : Bet wee n Mandria a nel Ayi os Nikolalls, roaels iel e a nel ga rri g li e. gab bro alld lim es to ll e, 27 Apr 19 9 1 (34- /5 77) (F ig. X4). This sp ee i es sh ows a enormo ll s kar yo l og i ca l va ri a ti on a nel so far, chrom osome numb ers or 2/1 = 14, 16 ,42.4 4.4 8 .54.56 , 5X . 59 , 60 , 62 , 64. 72 are repo rt cd (Afzal-Rafii 1979, Am in 1 972 , Bjarkqvi st & a l. 1 969 , Dahlgren & a l. 1971. Gade ll a & a l. 1 966 , Haqlle 19 82 , Haque & Gh os hal 1 980 , Kr alller & a l. 1 972. La ve & Kjellqvist 1 974 , Loon & al. 1971 , Marko va & I va n ova 1 982 , Natarajan 198 1, Patudin & al. 1975, QlIeiros 1 983 , R eese 1 95 7, Strid & Fran zé n 19 81 ). Tellcrillm kotscltyanlll1l P oec h 2n = 32 . Larnaka: Vavatsinia, road to L yt hrodhon da, roadside, v lli canic r oc ks, 770 m. 19 Apr 1 99 1 (20-772) (Fig. 85). The cy tol ogy of this Cyp rian endemie has not b ee n studi ed pr ev iously. Li/ia c eae A Llium juncelll1l Sm. /1 = 8. Paphos: Between Naia and Axylon. ga rrigue, lilll estone and ma rl -limestone. 350400 m, 22 Apr 19 9 1 (23-1081 )(F ig. 76 ). Our co unl of n = 8 is in agr ee ment with th e former report or a diplo id nllmber (2 /1 = 16) by Kar avo kyro ll & Tzanoudakis (1 99 1) who stlldi ed pianI mate ri al of Gr ee k provenance. Al/illm trifoliatlll1l Cirillo 2 /1 = 14. Paphos: Betw ee n El ed hio ll and Amarge li , gy psum, 360 m, 22 Apr 19 91 (241/ 20) (Fig. 86). A /liulI1 tr (f'o /i atu /' ll. a me mb er of A. see t. Moliu lII is fO llnd in th e M ed iterran ean regi on from S. lIaly eas lw ards. Diplo id planls or Illis speci es h ave b ee n repo rl e el pr ev iously only on ce by Tz ano uel a ki s & Kar avokyro u (1 992) from Cyp rus. A. tr!f oliatll/ll normally is a Iriplo id wilh 2/1 = 3x = 21 e hr omosomes ( Tz ano uel a ki s & Vosa 1 988 , T za no ll elak is 1 986 , Miehe li & a l. 19 81, 19 84, Ba rl olo & a l. 198 1) a nel a po ss ible h yb riel orig in in vo lv in g elianel letraplo iel A. of th e "sub l' i/losu lII -sub hir sutu lII "-complex was s li gges t eel by Miche li & a l. (1 984). Bellel1alia trifoliata (Ten.) Kunth 2 /1 = 8. Limasso l: Amath ll S. slony slopes. edge of ag ri cultural l and. wa ll s. ga rrig ll e. mari éll1lllim es tone , 10-40111. 14 Ap r 1 99 1 ( 11 -358) (F ig. 87). Our cO lmt or 2 1! = 8 co nfirms th e former reports by Bothmer & Be nt zer (1 973) a mi Bothmer & We nel elbo ( 1 98 1) who sluelieu Greek a nel Turkish pl a nt mate ri a l. It al so co nfinn s th e studi cs on plants or lInkn own ong ll1 by Feinbrlln (1 931{) and Ga rba ri & Tornadore (1 972) .
Bocconea I I - 1999 157 ... ;, . .. Figs. 85-90. Metaphases of root-t ip miloses of: 85, / éLlcriLl/Ii kotschyall//II/. 2/1 = 32; 86, AI/iLI/II trifo/iatulI/ . 2 /'1 = 1 4; 87, Be l/ eva lia tr(foliata . 2 11 = 8; 88, Hum ex cy prius . 2/'1 = 18; 89, ( )l e/amen cy priuII/ . 2/1 = 30; 90, Cye/amel1 persicuII/. 2/1 = 48 . - Scal e bar = IO pm.
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