Positive biocompatibility of several graphene derivatives with dopaminergic cells at long term culture
Rodríguez-Losada, Noela,Wendelbo, Rune,Arenas, Ernest,Aguirre-Gómez, José Ángel
- Published
- 2015-09-02
- Language
- en
Abstract
Carbon nanomaterial graphene (G) can form a three-dimensional porous structure with efficient bioconjugation and cell differentiation properties, providing a promising scaffold for neural regeneration. Aims: To study this putative new application of G, we cultured a clonal substantia nigra dopaminergic neuronal progenitor cell line (SN4741) in presence of G as scaffold. Methods: Cells were cultured in DMEM/10% FCS to about 80% confluence and incubated with different concentrations (0.001 to 1 mg/ml) of three chemically different G derivatives (G oxide (GO); partially reduced GO (PRGO) and fully reduced GO (FRGO)) and two different presentation matrixes as powder and films. Cell viability was measured by the MTT assay. To study cellular characterization, morphology and assessment of cell engraftment into G films, we analyzed the immunostaining of the neuronal marker NeuN, the anti-rat Beta-3-tubulin antibody, and the anti-rabbit DCX as immature neuronal marker. Lactate dehydrogenase was measured in the culture supernatant. Results: We found similar increase of survival and metabolism (30-40%) at low concentrations of PRGO and FRGO (0.05-0.01 mg/ml) compared with the higher concentration (1 mg/ml), no changes were seen in the GO group. PRGO or FRGO films showed an increased in the effective anchorage capacity to nest into the G matrix and in the maturation of the dopaminergic SN4741 cells. Conclusions: G scaffolds could offer a powerful platform for neural stem cells, direct cell conversion techniques and neural tissue engineering.
Full text
Positivebiocompatibilityofseveralgraphenederivativeswithdopaminergic cellsatlong termculture Noela Rodriguez‐Losada1,Rune Wendelbo2,Ernest Arenas4andJose A.Aguirre1 1 fhil Sh l fdi i iif l 290 lSi ORALCOMMUNICATION.C4:Graphene 1 Departmento f HumanP h ys i o l ogy, S c h oo l o f Me di c i ne,Un i vers i tyo f Ma l aga, 290 71Ma l aga, S pa i n 2AbalonyxAS,Forskningsveien 1,0373Oslo,Norway 3DepartmentofMedicalBiochemistryandBiophysics,Karolinska Institutet,SE‐17177Stockholm,Sweden Carbonnanomaterial graphene(G)canformathree‐dimensionalporousstructurewithefficientbioconjugation andcelldifferentiationproperties,providingapromisingscaffoldforneuralregeneration.Aims: Tostudythis putativenewapplicationofG,weculturedaclonal substantia nigra dopaminergic neuronalprogenitorcellline (SN4741)inpresenceofGasscaffold.Methods: CellswereculturedinDMEM/10%FCStoabout80% confluenceandincubatedwithdifferentconcentrations ( 0.001to1m g/ ml ) ofthreechemicall y differentG (g/ ) y derivatives(Goxide(GO);partiallyreducedGO(PRGO)andfullyreducedGO(FRGO))andtwodifferent presentationmatrixesaspowderandfilms.CellviabilitywasmeasuredbytheMTTassay.Tostudycellular characterization,morphologyandassessmentofcellengraftmentintoGfilms,weanalyzedtheimmunostaining oftheneuronalmarkerNeuN,theanti‐ratBeta‐3‐tubulinantibody,andtheanti‐rabbitDCXasimmature neuronalmarker.Lactatedehydrogenase wasmeasuredintheculturesupernatant.Results: Wefoundsimilar increaseofsurvivalandmetabolism(30‐40%)atlowconcentrationsofPRGOandFRGO(0.05‐0.01mg/ml) comparedwiththehigherconcentration(1mg/ml),nochangeswereseenintheGOgroup.PRGOorFRGOfilms showedanincreasedintheeffectiveanchoragecapacitytonestintotheGmatrixandinthematurationofthe dopaminer ic SN4741 cells Concl sions Gscaffolds co ld offer apo erf l platform for ne ral stem cells direct dopaminer g ic SN4741 cells . Concl u sions : G scaffolds co u ld offer a po w erf u l platform for ne u ral stem cells , direct cellconversiontechniquesandneuraltissueengineering. ThisworkhasbeensupportedbytheUniversityofMalaga,CampusdeExcelencia Internacional Andalucia Tech,Málaga,SpainandtheNorwegianResearchCouncil(grantnº215086)
