Increased Cardiac Workload in the Upright Posture in Men: Noninvasive Hemodynamics in Men Versus Women

Increased Cardiac Workload in the Upright Posture in Men: Noninvasive Hemodynamics in Men Versus Women Pauliina Kangas, MD; Anna Tahvanainen, MD, PhD; Antti Tikkakoski, MD; Jenni Koskela, MD, PhD; Marko Uitto, MSc; Jari Viik, MSc, PhD; Mika K€ ah€ onen, MD, PhD; Tiit K€ o€ obi, MD, PhD; Jukka Mustonen, MD, PhD; Ilkka P€ orsti, MD, PhD Background-—Men and women differ in the risk of cardiovascular disease, but the underlying mechanisms are not completely understood. We examined possible sex-related differences in supine and upright cardiovascular regulation. Methods and Results-—Hemodynamics were recorded from 167 men and 167 women of matching age (45 years) and body mass index (26.5) during passive head-up tilt. None had diabetes mellitus or cardiovascular disease other than hypertension or used antihypertensive medication. Whole-body impedance cardiography, tonometric radial blood pressure, and heart rate variability were analyzed. Results were adjusted for height, smoking, alcohol intake, mean arterial pressure, plasma lipids, and glucose. Supine hemodynamic differences were minor: Men had lower heart rate (4%) and higher stroke index (+7.5%) than women (P<0.05 for both). Upright systemic vascular resistance was lower (10%), but stroke index (+15%), cardiac index (+16%), and left cardiac work were clearly higher (+20%) in men than in women (P<0.001 for all). Corresponding results were observed in a subgroup of men and postmenopausal women (n=76, aged >55 years). Heart rate variability analyses showed higher low:high frequency ratios in supine (P<0.001) and upright (P=0.003) positions in men. Conclusions-—The foremost difference in cardiovascular regulation between sexes was higher upright hemodynamic workload for the heart in men, a finding not explained by known cardiovascular risk factors or hormonal differences before menopause. Heart rate variability analyses indicated higher sympathovagal balance in men regardless of body position. The deviations in upright hemodynamics could play a role in the differences in cardiovascular risk between men and women. Clinical Trial Registration-—URL: http://www.clinicaltrials.gov. Unique identifier: NCT01742702. (J Am Heart Assoc. 2016;5: e002883 doi: 10.1161/JAHA.115.002883) Key Words: cardiac output •hemodynamics •sex-specific The lifetime risk of cardiovascular disease (CVD) is close to 50% for persons aged 30 years without known CVD, 1 and it is the most common cause of death for both men and women. 2 The risk and the outcome of CVD, however, differ between sexes; particularly before middle age, morbidity and mortality caused by CVDs are higher in men. 3 In the Framingham Heart Study, the lifetime risk of coronary heart disease at age 40 years was about 49% for men and 32% for women. 4 These differences in CVD risk between sexes have been attributed to hormonal differences and to the fact that most of the risk factors are more favorable for women. 5 Distinct differences in cardiovascular regulation have been characterized between sexes. In early adulthood, pulse pressure is higher in men; however, at older ages, pulse pressure becomes higher in women. 6 The myocardial remodeling process in response to aging, pressure and volume overload, and myocardial infarction appears to be more favorable in women than in men. 7 In patients with ischemic heart disease, women may be more prone to myocardial ischemia in response to mental stress, whereas men may show higher increases in blood pressure (BP). 8 Taken together, these findings raise the question of whether further differences in cardiovascular regulation exist between men and women that could influence the hemodynamic load of the heart and subsequently affect the number of cardiovascular end points. Hypertension is more prevalent in young men than in young women, but after menopause, the risk of hypertension From the School of Medicine, University of Tampere, Finland (P.K., A. Tahvanainen, A. Tikkakoski, J.K., M.K., J.M., I.P.); Departments of Internal Medicine (A. Tahvanainen, J.K., J.M., I.P.) and Clinical Physiology (A. Tikkakoski, M.K., T.K.), Tampere University Hospital, Tampere, Finland; Department of Electronics and Communication Engineering, Tampere University of Technology, Tampere, Finland (M.U., J.V.). Correspondence to: Pauliina Kangas, MD, School of Medicine/Internal Medicine, University of Tampere, Tampere FIN-33014, Finland. E-mail: pauliina.kangas@fimnet.fi Received November 7, 2015; accepted May 23, 2016. ª2016 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. DOI: 10.1161/JAHA.115.002883 Journal of the American Heart Association 1 ORIGINAL RESEARCH by guest on February 7, 2017http://jaha.ahajournals.org/Downloaded from is significantly increased in women. 9 The lifetime burden of hypertension on the prevalence of CVDs is substantial. 1 In a survey in Finland performed in 2007, only 37% of the drugtreated hypertensive patients had normal BP (<140/ 90 mm Hg). 10 The suboptimal control of hypertension raises the question of whether individual characteristics and possible sex-related differences in the regulation of hemodynamics should be more carefully taken into account in the treatment of elevated BP. Although a major share of the active life is spent in the upright position, most reports on sex-related differences in hemodynamics have focused on resting conditions. 11–14 Only few studies have compared upright hemodynamics between sexes. 15–17 Similar upright increase in peripheral arterial resistance and decrease in cardiac output was reported in 9 men versus 8 women, 15 lower upright splanchnic vasoconstriction but corresponding decrease in cardiac output was reported in 14 women versus 16 men, 17 and corresponding upright increase in forearm vascular resistance was observed in 48 women versus 41 men. 16 In most of the previous studies, the control of cardiac autonomic tone between sexes was evaluated by the use of heart rate variability (HRV). In the majority of these reports, sympathovagal balance was reported to be higher in men, regardless of whether the participants were in supine, sitting, or standing position. 16,18– 20 The previous studies comparing hemodynamics between sexes were performed with small numbers of participants, and the groups have not been uniform in age and body mass index (BMI). 8,15–17 Therapies based on individual sex-related characteristics could improve outcomes in the treatment of CVDs like hypertension, 21 and personalized approaches in the treatment of medical conditions are keenly sought. 22–24 Because only limited information exists about the upright regulation of the cardiovascular system between sexes, we compared the hemodynamic responses to passive head-up tilt in 167 men versus 167 women with corresponding age and BMI. The main findings were verified in a larger group composed of an additional 313 men and 231 women. A total of 480 men and 398 women were examined. Methods Study Participants All participants were in an ongoing study of hemodynamics in volunteers, with the primary aim of examining the hemodynamic changes in primary and secondary hypertension and normotensive control participants in supine and upright positions (DYNAMIC study; ClinicalTrials.gov identifier NCT01742702). Participants were enrolled from the patients treated at Tampere University Hospital. An announcement of recruitment was distributed at Tampere University Hospital, the University of Tampere, offices of local occupational health care providers, and Varala Sports Institute, and 2 announcements were published in a newspaper. Those who agreed to participate were recruited in the order in which their contact information was available to the research nurses. By the time of the present analysis, 878 participants (480 men and 398 women) had undergone hemodynamic measurements; of those, 615 were without medications with direct influence on cardiovascular function. The remaining participants included medicated patients with hypertension, chronic renal disease, diabetes mellitus, and atherosclerotic vascular disease. All participants were interviewed to record lifestyle habits, family history, and medical history. Clinical examination of cardiovascular status and routine laboratory tests were performed. For the main analysis of the present study, participants with antihypertensive medication, coronary heart disease, or previous myocardial infarction, diagnosis of diabetes mellitus, cardiac insufficiency, atherosclerotic vascular disease, renal disease, or cerebrovascular disease were excluded. In addition, all participants using medications with potential influence on hemodynamics (eg, a 1 -adrenoceptor blockers for prostate problems, b 2 -adrenoceptor agonists, digoxin, and topical b-blockers for glaucoma) were excluded. 25 To avoid confounding caused by differences in age and body weight, 26– 29 the following inclusion protocol was applied: A female participant was chosen, followed by selection of a male participant with corresponding age (difference in age ≤3 years) and BMI (difference ≤1.5 units). Using this approach, 334 participants aged 21 to 67 years were included (Table 1). Overall, 114 (34%) of the 334 persons used some medication (Table 2). Forty female participants used systemic estrogen, progestin, or their combination (for contraception or hormone replacement therapy), and 1 participant used tibolone. Eight participants were taking statins, and 11 euthyroid participants were on a stable dose of thyroid hormone. Other medications (acetylsalicylic acid, drugs for mental problems, antihistamines, proton pump inhibitors, intranasal or inhaled corticosteroid for asthma or allergy) were used by the study population. One participant who was physically well and symptomless was treated with warfarin for antiphospholipid syndrome. Additional analyses were performed in groups in which (1) all participants using systemic female hormones or statins were excluded, (2) all participants were aged ≥55 years, and (3) all 878 participants who participated in the recording of hemodynamics were included. The participants gave written informed consent, and the study was approved by the ethics committee of Tampere University Hospital (study code R06086M). DOI: 10.1161/JAHA.115.002883 Journal of the American Heart Association 2 Upright Hemodynamics in Men and Women Kangas et al ORIGINAL RESEARCH by guest on February 7, 2017http://jaha.ahajournals.org/Downloaded from Hemodynamic Measurements Hemodynamic measurements were performed in a temperature-controlled laboratory. The participants were advised to refrain from using caffeine-containing products, smoking, and eating heavy meals for at least 4 hours and from drinking alcohol for at least 24 hours prior to the recording. While the participants were resting supine on a tilt table, the electrodes for impedance cardiography were placed on the body surface, a tonometric sensor for radial artery BP on the left wrist (Colin BP-508T; Colin Medical Instruments Corp) and an oscillometric brachial cuff for BP calibration on the right upper arm. 25,26,31 The left arm with the tonometric sensor was abducted to 90°in a support that held the arm at the level of the heart in supine and upright positions. Estimation of central aortic BP was performed by mathematical transformation of radial tonometry pressure with the SphygmoCor pulse wave monitoring system (SpygmoCor PWMx; Atcor Medical) using a validated transfer function. 32 Table 1. Clinical and Metabolic Characteristics in the Study Groups Men (n=167) Women (n=167) PValue Age, y 4512 4511 0.967 Body mass index, kg/m 2 26.53.7 26.63.8 0.898 Height, cm 1806 1666<0.001 Weight, kg 8812 7311 <0.001 Systolic blood pressure, mm Hg 13217 12718 0.006 Diastolic blood pressure, mm Hg 7512 7212 0.026 Smoking Current 51 (30.5) 46 (27.5) 0.547 Previous 22 (13.2) 17 (10.2) 0.394 Never 94 (56.3) 10 (62.3) 0.265 Alcohol, standard drinks per week 4(1–10) 2 (0–4) <0.001 Creatinine, lmol/L 8212 659<0.001 Cystatin-C, mg/L 0.870.14 0.800.14 <0.001 eGFR, mL/min per 1.73 m 2 99.214.5 99.314.0 0.956 Total cholesterol, mmol/L 5.11.0 5.11.0 0.804 LDL cholesterol, mmol/L 3.11.0 2.80.9 0.021 HDL cholesterol, mmol/L 1.40.3 1.80.4 <0.001 Triglycerides, mmol/L 1.3 (0.7–1.5) 1.1 (0.7–1.3) 0.007 Fasting plasma glucose, mmol/L 5.50.5 5.30.5 <0.001 Cornell voltage product in ECG, ms9mm 1638615 1621509 0.779 Values are meansSD except for smoking, which shows the number of participants and percentages, and for alcohol intake and triglycerides, which are shown as medians (lower and upper quartiles) due to skewed distribution. eGFR indicates estimated glomerulus filtration rate 30 ; HDL, high-density lipoprotein; LDL, low-density lipoprotein. Table 2. Medications Used Regularly by the Study Participants (Number of Participants With Each Type of Medication) Medication Men (n=167) Women (n=167) Acetylsalicylic acid 3 0 Acyclovir 0 1 Allopurinol 0 1 Amitriptyline 0 1 Amoxicillin 0 1 Antidepressant (SSRI or SNRI) 3 12 Antihistamine 2 5 Doxycycline (low dose) 1 0 Ezetimibe 1 0 Female hormones Systemic (including tibolone) 0 41 Topical 0 3 Glucosamine 3 3 Intranasal or inhaled corticosteroid 2 6 Isotretinoin 0 1 Letrozole 0 1 Levomepromazine 0 1 Levonorgestrel via intrauterine device 0 16 Liothyronine 0 1 Lymecycline 1 0 Mefloquine 0 1 Melatonin 1 1 Mepacrine 1 0 Nonsteroidal anti-inflammatory drug 1 3 Oxybutynine 0 1 Pramipexole 0 1 Pregabalin 1 1 Proton pump inhibitor 3 4 Quetiapine 0 1 Statin 7 1 Thyroxine 0 10 Valproate 0 1 Vitamin D supplementation 6 11 Warfarin 1 0 SNRI indicates serotonin–norepinephrine reuptake inhibitor; SSRI, selective serotonin reuptake inhibitor. DOI: 10.1161/JAHA.115.002883 Journal of the American Heart Association 3 Upright Hemodynamics in Men and Women Kangas et al ORIGINAL RESEARCH by guest on February 7, 2017http://jaha.ahajournals.org/Downloaded from A whole-body impedance cardiography device (CircMon; JR Medical Ltd) that records changes in body electrical impedance during cardiac cycles was used to determine heart rate, stroke volume, and cardiac output. 25,33–35 The description of the method and electrode configuration was reported previously. 25,33–35 The values were normalized to body surface area and expressed as cardiac index, stroke index, systemic vascular resistance index (SVRI), and left cardiac work index (LCWI). SVRI was calculated from the radial BP signal and cardiac index measured by the CircMon device. LCWI (in kg9m/min per m 2 ) was calculated using following formula: 0.01439(mean aortic pressure–pulmonary artery occlusion pressure)9cardiac index. Pulmonary artery occlusion pressure was assumed to be 6 mm Hg (normal), and 0.0143 is the conversion factor of pressure from millimeters of mercury to centimeters of water, volume to density of blood (in kg/L), and centimeters to meters. 36,37 The stroke volume and cardiac output measured using CircMon whole-body impedance cardiography agree with values measured using 3-dimensional ultrasound and the thermodilution method, respectively, and agreement with the latter has been shown in both supine and upright positions. 34,36,38 An introductory head-up tilt was performed before the recordings. The actual measurement consisted of 3 consecutive 5-minute periods with continuous capture of data: 5 minutes supine, 5 minutes of passive head-up tilt to 60°, and 5 minutes supine. The repeatability and reproducibility of the supine and upright measurements has been shown to be good. 31 Evaluation of Cardiac Autonomic Tone HRV analysis from electrocardiograms was used to assess cardiac autonomic tone. The electrocardiograms were recorded by the CircMon device at a 200-Hz sampling rate, and data were analyzed using Matlab software (MathWorks Inc). Normal R-R intervals were recognized, and a beat was considered ectopic if the interval differed >20% from the previous values. The artifacts were processed using the cubic spline interpolation method. 39 Because the data were collected from short-term recordings, the frequency domain method was applied. 40 The following variables were calculated from the recordings in supine (0–5 minutes) and upright (5–10 minutes) positions using the fast Fourier transformation method: (1) total power, (2) power in the low-frequency (LF) range (0.04–0.15 Hz), (3) power in the high-frequency (HF) range (0.15–0.40 Hz), and (4) LF/HF ratio. 40 Laboratory Tests Blood samples were obtained after about 12 hours of fasting. Plasma glucose, creatinine, cystatin C, triglyceride, and total and highand low-density lipoprotein cholesterol concentrations were determined using the Cobas Integra 700/800 or Cobas 6000, module c501 (F. Hoffmann-La Roche Ltd), and blood cell count was performed by an ADVIA 120 or 2120 system (Siemens Healthcare GmbH). In some participants, low-density lipoprotein was calculated using the Friedewald formula. 41 Creatinineand cystatin C–based estimated glomerular filtration rate was calculated using the Chronic Kidney Disease Epidemiology Collaboration formula. 30 A standard 12-lead electrocardiogram was recorded, and left ventricular mass was evaluated using the Cornell voltage QRS duration product. 21 All laboratory values were missing from 3 participants, and low-density lipoprotein cholesterol values were missing from an additional 2 participants. Statistical Analyses The characteristics of men versus women were compared using an independent samples ttest, and smoking habits (current, previous and never) were compared using the Pearson chi-square test. Hemodynamic differences in supine and upright positions were examined using ANOVA for repeated measures, and the changes in hemodynamic values from supine to upright position (average values of last 3 minutes before and last 3 minutes during the head-up tilt 25 ) and HRV differences were analyzed using an independent samples ttest and ANOVA with possible confounding factors as covariates. Average BP values during the last 3 minutes of the first supine period were applied for the clinical characterization of the BP of participants (Table 1). The analyses were adjusted for smoking habits, alcohol intake (standard doses per week), fasting plasma glucose, triglycerides, highand low-density lipoprotein cholesterol, height, and mean arterial pressure, as appropriate (the adjusting variable was not used if it was included in the formula of the variable of interest). HRV analyses were also adjusted for heart rate. 42 In the adjusted analyses, current smoking as cigarettes per day was applied as a continuous variable, whereas in the tables, the smoking habits were described as current, previous, and never smoker. Due to missing data among the main group of interest (334 participants), the number of participants in the adjusted analyses ranged from 311 to 324, with at least 153 men and 158 women in all analyses. The skewed distributions of triglycerides, total power, LF power, HF power, and LF/HF ratio were logarithmically transformed before statistical analyses. Variables with normal distribution were reported as means and standard deviations (SD) or 95% CIs of the means; skewed distributions were reported as medians, lower and upper quartiles, and range; and categorical variables were reported as numbers of participants and percentages. All testing was 2-sided, and P DOI: 10.1161/JAHA.115.002883 Journal of the American Heart Association 4 Upright Hemodynamics in Men and Women Kangas et al ORIGINAL RESEARCH by guest on February 7, 2017http://jaha.ahajournals.org/Downloaded from values <0.05 were considered statistically significant. The data were analyzed using SPSS 17.0 (IBM Corp). Results Study Population The general characteristics of the study participants are presented in Table 1. Because of the inclusion protocol, men and women were well matched for age and BMI. In addition, there were no significant differences in smoking status, estimated glomerular filtration rate, 30 total cholesterol, and Cornell voltage product 21 between the 167 men and 167 women. Men, however, were characterized by somewhat higher systolic and diastolic BP, alcohol intake, and higher fasting plasma creatinine, cystatin C, low-density lipoprotein cholesterol, triglyceride, and glucose concentration and lower high-density lipoprotein cholesterol concentration than women. Supine and Upright Hemodynamics Men had higher supine mean arterial pressure, stroke index, and LCWI and lower heart rate than women in unadjusted analyses (Figure 1A–1C and 1E). Supine cardiac index and SVRI did not differ between sexes (Figure 1D and 1F). After adjusting for height, smoking habits, alcohol intake, mean arterial pressure, lowand high-density lipoprotein cholesterol, triglycerides, and glucose, only the differences in supine heart rate and stroke index for men and women remained significant (Figure 1B and 1C). During passive head-up tilt to 60°, men had higher mean arterial pressure, stroke index, cardiac index, and LCWI (Figure 1A, 1C, 1D, and 1E) and lower SVRI (Figure 1F) than women in unadjusted analyses. Upright heart rate did not differ between men and women (Figure 1B). In adjusted analyses, with the exception of mean arterial pressure, all of the above differences in upright hemodynamics for men and women remained significant (Figure 1A and 1C–1F). The magnitude of the changes in hemodynamic variables in response to upright posture was also analyzed (see Methods). Unadjusted analyses showed a greater increase in heart rate (P<0.001); less decrease in stroke index, cardiac index, and LCWI (P<0.05 for all); and less increase in SVRI (P<0.001) in response to head-up tilt in men than in women. A similar increase in mean arterial pressure was observed in both sexes (P=0.812). In adjusted analyses, the differences in the upright changes in cardiac index, LCWI, and SVRI remained significant (P<0.01 for all), whereas the changes in heart rate and stroke index were no longer different between men and women (P=0.600 and P=0.694, respectively). HRV in Supine and Upright Positions In supine and upright positions, there were no significant differences in total power and in power in the LF range in unadjusted analyses (Figure 2A and 2B), whereas men had lower power in the HF range and higher LF/HF ratios than women (Figure 2C and 2D). In adjusted analyses, the upright differences in the HF component were no longer significant (Figure 2C), but supine HF power was lower and both supine and upright LF/HF ratios remained higher in men than in women (Figure 2C and 2D) Supine and Upright Hemodynamics in Additional Analyses Three additional analyses of hemodynamic changes in response to head-up tilt were performed: (1) All participants using systemic female hormones and statins were excluded (n=285) (Figure 3); (2) all included participants were aged ≥55 years, and none used systemic female hormones (n=76) (Figure 4); (3) all participants who participated in hemodynamic recordings were included (n=878) (Figure 5). The last group was composed of 615 participants who were without medications with direct influence on cardiovascular function and 263 medicated patients with hypertension, chronic renal disease, diabetes mellitus, or atherosclerotic vascular disease. The outcome of all of these additional analyses was that the sex-related differences in upright hemodynamics were very similar to those observed in the 167 men and 167 women with matching age and BMI. The results clearly showed a higher upright hemodynamic workload for the heart in men (Figures 3 through 5). Discussion Several previous studies examined the differences in cardiovascular function between sexes, 6–8,16,18–20,43 but only a few reports compared the upright hemodynamics between sexes. 15–17,44,45 Although head-up tilt for 5 minutes is a short period of observation, we found that upright hemodynamic adaptation differed between men and women. In men, cardiac index and left cardiac work decreased less in the upright position than in women, whereas men also showed lower upright increase in systemic vascular resistance than women. The net outcome was that the upright hemodynamic workload of the heart was higher in men than in women. To gain functional insight about the cardiovascular system, we applied a head-up tilt protocol to induce changes in autonomic tone, cardiac function, and peripheral arterial resistance in the study participants. 25,26,31,35 Because age and excess body weight have major influences on DOI: 10.1161/JAHA.115.002883 Journal of the American Heart Association 5 Upright Hemodynamics in Men and Women Kangas et al ORIGINAL RESEARCH by guest on February 7, 2017http://jaha.ahajournals.org/Downloaded from hemodynamics, 26–29 we initially performed analyses in 167 men and 167 women with corresponding age and BMI; however, the findings showing higher cardiac workload during head-up tilt in men remained similar in participants not using statins or hormone replacement therapy, in participants aged ≥55 years, and in a large group composed of 878 participants. Figure 1. Line graphs show mean arterial pressure (A), heart rate (B), stroke index (C), cardiac index (D), left cardiac work index (E), and systemic vascular resistance index (F) in 167 men and 167 women during supine position and passive head-up tilt (means and 95% CIs of the mean). Pvalues denote differences between sexes in unadjusted analysis and in analyses adjusted for lowand high-density lipoprotein cholesterol, triglycerides, glucose, mean arterial pressure, smoking habits, alcohol intake, and height. DOI: 10.1161/JAHA.115.002883 Journal of the American Heart Association 6 Upright Hemodynamics in Men and Women Kangas et al ORIGINAL RESEARCH by guest on February 7, 2017http://jaha.ahajournals.org/Downloaded from We examined cardiac autonomic tone using the frequency domain analyses of HRV. 16,18–20 The HF component reflects cardiac parasympathetic activity, 46–48 whereas the LF component predominantly reflects sympathetic activity, although it contains parasympathetic contributions. 40,46,48 The LF/HF ratio is a marker of sympathovagal balance, 40,46 but this matter remains controversial, and conclusions should be drawn with caution. 47,48 In the present study, the frequency domain analysis of HRV showed higher LF/HF ratios in men both supine and upright, suggesting increased sympathovagal balance. The differences in cardiac autonomic tone largely resulted from decreased HF power, namely, lower cardiac parasympathetic tone in men than women. There has been controversy about whether the indices of HRV are higher in men or in women. In participants aged <30 years, the time domain measures of HRV were higher in men than in women 49 ; however, most published reports on frequency domain measures of HRV have found that the values of LF power are higher in men, 18–20,50 whereas the values of HF power are higher in women. 16,19,50 Higher LF/HF ratios during supine and standing positions have been reported in 156 men versus 206 women, 20 and the majority of reports have suggested that sympathovagal balance is higher in men. 15,16,18–20,43,50–52 The present findings stress the role of differences in cardiac parasympathetic regulation between sexes. Higher sympathovagal balance in men agrees with higher upright cardiac workload but raises questions about lower Figure 2. Box plots of heart rate variability in men and women during 5 minutes in supine and upright positions: total power (A), LF power (B), HF power (C), and LF/HF ratio (D) (median [line inside box], 25th to 75th percentile [box], and range [whiskers); outliers were excluded from the figure but were included in the statistics). Pvalues denote differences between sexes in unadjusted analysis and in analyses adjusted for lowand high-density lipoprotein cholesterol, triglycerides, glucose, heart rate, 42 mean arterial pressure, smoking habits, alcohol intake, and height. HR indicates high frequency; LF, low frequency. DOI: 10.1161/JAHA.115.002883 Journal of the American Heart Association 7 Upright Hemodynamics in Men and Women Kangas et al ORIGINAL RESEARCH by guest on February 7, 2017http://jaha.ahajournals.org/Downloaded from upright SVRI in men. Both arteries and veins are innervated by autonomic nerves. Autonomic innervation of the resistance arteries consists of sympathetic and sensory–motor nerves, whereas parasympathetic vascular innervation is nonexistent or very limited. 53 If higher sympathovagal balance would be transmitted to the resistance arteries similarly in men and women, higher cardiac output would result in an excessive upright rise of arterial pressure in men, with subsequent activation of baroreceptors. 54 This would limit the increase in vascular resistance, and the comparison of baroreflexes between men and women is a subject for further studies. Previously, baroreceptor sensitivity during orthostasis has been reported to be higher in men than in women. 15 Although sympathetic nerves are predominantly vasoconstrictor nerves, they can induce vasodilation in skeletal muscles, 53 and the net effect of sympathetic stimulation on peripheral resistance depends on the circulatory balance between skeletal muscles and other organs. Studies of muscle sympathetic nerve activity have shown that the association of sympathetic tone with hemodynamics is not straightforward. 14,15 In younger men and women (aged <40 years), no relationship was found between muscle sympathetic nerve activity and BP, although a clear relationship was observed in older participants (aged ≥40 years). 14 In younger men, muscle sympathetic nerve activity was positively related to peripheral vascular resistance and negatively related to cardiac output, whereas in younger women, no relationship was found between muscle sympathetic nerve activity and peripheral vascular resistance Figure 3. Participants using statins or hormone replacement therapy were excluded. Line graphs show mean arterial pressure (A), cardiac index (B), left cardiac work index (C), and systemic vascular resistance index (D) in 160 men and 125 women during supine position and passive head-up tilt (means and 95% CIs of the mean). Pvalues denote differences between sexes in unadjusted analysis and in analyses adjusted for lowand high-density lipoprotein cholesterol, triglycerides, glucose, mean arterial pressure, smoking habits, alcohol intake, age, and body mass index. DOI: 10.1161/JAHA.115.002883 Journal of the American Heart Association 8 Upright Hemodynamics in Men and Women Kangas et al ORIGINAL RESEARCH by guest on February 7, 2017http://jaha.ahajournals.org/Downloaded from or cardiac output. 14 Finally, autonomic innervation is not the sole regulator of resistance arterial tone; endotheliumdependent mechanisms, myogenic regulation, and humoral factors also contribute to vascular resistance. 53,55 The present findings provide another putative explanation for the higher risk of cardiac events in men. Coronary heart disease and myocardial infarction account for a third to half of the initial presentations of CVD, and male sex particularly predisposes to myocardial infarction in those aged <60 years. 56 The more unfavorable upright hemodynamic load of the heart could lead to earlier clinical manifestation of coronary heart disease in men than in women. Of note, the coronary findings in those who experienced sudden death also differ between sexes. In 442 cases of sudden coronary death, the prevalence of acute thrombosis and plaque rupture was higher in men than in women (53% versus 46% and 71% versus 33%, respectively), whereas plaque erosion as a cause of thrombosis was less frequent in men than in women (24% versus 58%). 57 Finally, even the prognosis of heart failure has been reported to be worse in men than in women. 58,59 This is not explained by the etiology of the heart failure or by differences in left ventricular ejection fraction. 60 Differences in the upright hemodynamic load of the heart could partially explain some of the aforementioned differences in cardiovascular risk between men and women. An obvious difference between men and women is hormonal function, and both endogenous and exogenous sex hormones have effects on the vasculature. 9,61 Figure 4. All included participants aged ≥55 years. Line graphs show mean arterial pressure (A), cardiac index (B), left cardiac work index (C), and systemic vascular resistance index (D) in 43 men and 33 women (no hormone replacement therapy in use) during supine position and passive head-up tilt (means and 95% CIs of the mean). Pvalues denote differences between sexes in unadjusted analysis and in analyses adjusted for lowand high-density lipoprotein cholesterol, triglycerides, glucose, mean arterial pressure, smoking habits, alcohol intake, age, and body mass index. DOI: 10.1161/JAHA.115.002883 Journal of the American Heart Association 9 Upright Hemodynamics in Men and Women Kangas et al ORIGINAL RESEARCH by guest on February 7, 2017http://jaha.ahajournals.org/Downloaded from