Timing of puberty and reserve capacity in adolescence as pathways to educational level in adulthood : a longitudinal study

Full Terms & Conditions of access and use can be found at https://www.tandfonline.com/action/journalInformation?journalCode=iahb20 Annals of Human Biology ISSN: 0301-4460 (Print) 1464-5033 (Online) Journal homepage: https://www.tandfonline.com/loi/iahb20 Timing of puberty and reserve capacity in adolescence as pathways to educational level in adulthood—a longitudinal study Paulyn Jean Acacio-Claro, Leena Kristiina Koivusilta, David Teye Doku & Arja Hannele Rimpelä To cite this article: Paulyn Jean Acacio-Claro, Leena Kristiina Koivusilta, David Teye Doku & Arja Hannele Rimpelä (2019) Timing of puberty and reserve capacity in adolescence as pathways to educational level in adulthood—a longitudinal study, Annals of Human Biology, 46:1, 35-45, DOI: 10.1080/03014460.2019.1596311 To link to this article: https://doi.org/10.1080/03014460.2019.1596311 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. Accepted author version posted online: 19 Mar 2019. Published online: 24 Apr 2019. Submit your article to this journal Article views: 185 View Crossmark data RESEARCH PAPER Timing of puberty and reserve capacity in adolescence as pathways to educational level in adulthood—a longitudinal study Paulyn Jean Acacio-Claro a , Leena Kristiina Koivusilta b , David Teye Doku a,c and Arja Hannele Rimpel€ a a,d a Faculty of Social Sciences, Unit of Health Sciences, Tampere University and PERLA (Tampere Centre for Childhood, Youth and Family Research), Tampere University, Tampere, Finland; b Department of Social Research, Faculty of Social Sciences, University of Turku, Turku, Finland; c Department of Population and Health, University of Cape Coast, Cape Coast, Ghana; d Department of Adolescent Psychiatry, Tampere University Hospital, Nokia, Finland ABSTRACT Background: Family socioeconomic status (SES) is related to a child’s educational success. Intermediate pathways for this relationship, such as through pubertal timing and reserve capacity, occur in adolescence. Aim: To study whether family SES affects a child’s adult education through a psychosocial and behavioural pathway (reserve capacity) and/or a biological pathway (pubertal timing) or only through school achievement in adolescence. Subjects and methods: Finnish adolescents sampled in five cross-sectional surveys from 1985 to 1995 (n¼37,876) were followed through the Registry of Completed Education and Degrees until 2009, when they were 29–43years old. Family SES data also came from this registry. Structural equation modelling adjusted for ages at baseline and follow-up was used. Results: Low family SES increased the probability of low adult education, delayed pubertal timing (in boys), weak reserve capacity and low school achievement. Reserve capacity and school achievement directly affected adult education and mediated the relationship of family SES with the outcome. Delayed pubertal timing predicted low adult education, except when school achievement was added to the model. Conclusions: The results show that family SES affects the child’s adult education level through psychosocial and biobehavioural pathways, but the biological pathway is mediated by school achievement. ARTICLE HISTORY Received 10 September 2018 Revised 25 February 2019 Accepted 1 March 2019 KEYWORDS Socioeconomic status; puberty; education; reserve capacity; school achievement Introduction From a developmental perspective, adolescence has a unique position in the life course because it could either lessen or aggravate the impact of early childhood disadvantages on adult outcomes (Johnson et al. 2011). Rapid biological and social changes such as puberty and increasing autonomy from one’s family, as well as school, peer and other environmental influences, shape socio-emotional development and lead to formation and adoption of new behaviours (Viner et al. 2012), consequently affecting ‘successful’transitions into adulthood (Johnson et al. 2011). Hence, intermediate pathways from childhood exposures to educational trajectories may be elucidated in adolescence. In early life, the socioeconomic status (SES) of the family is an important exposure which has been strongly linked to various developmental outcomes of children and adolescents, particularly educational attainment (Conger et al. 2010; Merritt and Buboltz 2015; Acacio-Claro et al. 2018). Previous research focusing on SES as a predictor of child development explained that such links probably occur through family dynamics, parenting practices and investments for children (Martin et al. 2010). Accordingly, higher SES families tend to invest more in the health and education of their children than lower SES families do (Conger et al. 2010). Research has also shown that economic hardship affects relationships between parents and children, leading to poor parenting practices or poor communication in the family, which influence the cognitive, emotional and behavioural development of children (Kroenke 2008; Conger et al. 2010). During adolescence, one salient marker of development with effects likely persisting until adulthood is puberty, and its timing has been extensively studied due to its complex familial and environmental causes (Parent et al. 2003; Euling et al. 2008; Golub et al. 2008; Johnson et al. 2011; Graber 2013). The physical, behavioural and hormonal effects of puberty, particularly when occurring earlier or later than in one’s age-mates, bring psychological and adjustment issues linked to elevated symptomatology and risks of psychopathology during adolescence and other disorders in adulthood (Golub et al. 2008; Graber 2013). Higher rates of depressive symptoms, especially in girls (Copeland et al. 2010; Keenan et al. 2014), risky health behaviours (Koivusilta and Rimpel€ a CONTACT Paulyn Jean Acacio-Claro [email protected] Faculty of Social Sciences, Unit of Health Sciences, Tampere University and PERLA (Tampere Centre for Childhood, Youth and Family Research), Tampere University, PO Box 100, 33014, Tampere, Finland ß2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ANNALS OF HUMAN BIOLOGY 2019, VOL. 46, NO. 1, 35–45 https://doi.org/10.1080/03014460.2019.1596311 2006; Golub et al. 2008; Downing and Bellis 2009; Graber 2013) and higher risks for developing cardiovascular disease (Golub et al. 2008; Jacobsen et al. 2009; Lakshman et al. 2009; Bleil et al. 2013), type 2 diabetes, breast and testicular cancers (Golub et al. 2008) were associated with early maturation. On the other hand, late maturation increased fracture risk (Zhu and Chan 2017) and psychopathology in boys in terms of higher rates of depressive symptoms and disruptive behaviours (Graber 2013; Zhu and Chan 2017). Aside from its health impact, recent evidence suggests that pubertal timing has cognitive effects which may be reflected in academic performance (Cavanagh et al. 2007; Martin and Steinbeck 2017) and educational outcomes (Koivusilta and Rimpel€ a 2004; Koerselman and Pekkarinen 2017), influencing socioeconomic conditions in adulthood (Johnson et al. 2011; Koerselman and Pekkarinen 2017). Secular changes observed regarding pubertal timing have been attributed mainly to improvements in nutrition and health, including an increase in body fat (de Muinich Keizer and Mul 2001; Parent et al. 2003). Pubertal timing is also influenced by certain gene regulators, gender, race/ethnicity (Obeidallah et al. 2000; Parent et al. 2003; Euling et al. 2008) and exposure to endocrine disrupting chemicals (Parent et al. 2003; Aksglaede et al. 2008). A stressful family environment characterised by family conflict (Bleil et al. 2013) and stressful life events (Sun et al. 2017), for example, father absenteeism, divorce and single parent families (Bellis et al. 2006) is likewise linked to altered pubertal timing. Notably, research has documented mixed findings of socioeconomic inequalities in timing of puberty (de Muinich Keizer and Mul 2001; Parent et al. 2003; Downing and Bellis 2009; JamesTodd et al. 2010; Sun et al. 2017). On one hand, high SES or ‘privileged conditions’were shown to have shifted pubertal timing towards earlier ages (de Muinich Keizer and Mul 2001; Parent et al. 2003), possibly due to improved childhood health status (de Muinich Keizer and Mul 2001; Bellis et al. 2006) and nutrition (Parent et al. 2003; Bellis et al. 2006; Kyweluk et al. 2018). On the other hand, low SES or childhood socioeconomic disadvantage was also found to accelerate pubertal onset (James-Todd et al. 2010; Sun et al. 2017) due to environmental stress, which hastens reproductive maturation (Obeidallah et al. 2000; James-Todd et al. 2010; Xu et al. 2018). The mechanisms through which pubertal timing occurs and causes adverse health outcomes likely represent the interplay of socioeconomic, psychosocial and biobehavioural pathways in the life-course (Gallo et al. 2009; Matthews and Gallo, 2011). An integrative framework overarching this is the reserve capacity model proposed by Gallo and Matthews (2003). This model posits that low SES increases one’s exposure to environmental stressors and depletes psychosocial resources such as self-efficacy, mastery and social support, triggering negative emotional and physiological responses, affecting health via altered biological and behavioural pathways (Gallo et al. 2009; Matthews et al. 2010; Matthews and Gallo 2011). Initially designed to understand how the psychosocial pathway links SES with physical health (Gallo and Matthews 2003), research which tested this model among adults produced inconclusive results about the hypothesised relationships (Matthews et al. 2010). However, studies conducted among children and adolescents yielded clearer directions on the connections of childhood SES and adult health outcomes through reserve capacity and biobehavioural pathways (Matthews et al. 2010). In addition, low SES and poor psychosocial functioning early in life placed children and adolescents at risk of lower educational outcomes compared to those with high SES and/or strong reserve capacity (Matthews et al. 2010). We adopt this framework to assess whether pubertal timing and reserve capacity are such pathways through which SES influences educational trajectories. We added health-promoting behaviours, namely tooth brushing and physical activity, to the reserve capacity framework, as these underlie psychosocial resources such as perceived control and selfefficacy (Robbins et al. 2004; Cinar et al. 2009; Pakpour and Sniehotta 2012). Moreover, both behaviours were found to serve as pathways from childhood socioeconomic position to adult education level (Koivusilta et al. 2013), hence we included these variables in the present study. In this study, reserve capacity covers three dimensions, namely: perceived health, health-promoting behaviour and social support; with each dimension shown to independently predict adult education (Acacio-Claro et al. 2018). We also add another factor, school achievement, as several studies have shown this to be one of the strongest predictors of adult education (Slominski et al. 2011; Brekke 2015; Acacio-Claro et al. 2018). Further, we propose that the pathways occurring in adolescence might interact with each other to affect adult education (Figure 1). In general, we studied whether family SES affects a child’s adult education through a psychosocial and behavioural pathway (reserve capacity) and/or a biological pathway (timing of puberty) or only through school achievement in adolescence. Specifically, we want to test the following hypotheses: (1) family SES is related to pubertal timing, reserve capacity and school achievement; (2) pubertal timing and reserve capacity influence adult education level; and (3) family SES relates to adult education level directly and indirectly (i.e. mediated by any of the adolescent pathways). Understanding these mechanisms will help clarify the links among SES, adolescent pathways and adult education and point to new ways of supporting young people to achieve their full potential in learning—a recognised important life stage transition (Viner et al. 2012). Subjects and methods Study design and sample A longitudinal study design was constructed using two data sources linked through unique national personal identification numbers. Baseline data were obtained from the Adolescent Health and Lifestyle Surveys (AHLS) of 1985, 1987, 1991, 1993 and 1995. The AHLS monitors the health and health-related lifestyle of adolescents in Finland. Nationally representative samples of 14-, 16and 18-year-old Finns born on certain days in June, July and August between 36 P. J. ACACIO-CLARO ET AL. 1966 and 1980 were drawn for each study year from the Population Register Centre. Even though the AHLS has been conducted biennially since 1977, the variables suitable for measuring reserve capacity were included only in the abovementioned years. A self-administered questionnaire, to be voluntarily answered, was sent by post in February, followed by two re-inquiries to non-respondents. The overall response rate was 79.1% (n¼37,876), with 71.9% (n¼17,531) for boys and 86.6% (n¼20,345) for girls, respectively. Follow-up data on adult education, as well as socioeconomic information for the parents of AHLS participants, were obtained from the Registry of Completed Education and Degrees of Statistics Finland. The data from Statistics Finland covered censuses every fifth year from 1970 to 1995, and yearly registry data from 2000 until the end of 2009. At the end of 2009, the AHLS participants were aged 29–43 years. Statistics Finland performed the data linkage according to a contract specifying the rights and duties of both parties. The Institutional Review Board of Statistics Finland and the Data Protection Ombudsman approved the study protocol. The Joint Commission on Ethics of the University of Turku and the Turku University Hospital also stated that no human rights were violated in the research protocol and approved it. Identification of the study participants was withheld from the investigators at all stages of the study. Variables from Statistics Finland Adult education level of the survey respondents This is the main outcome of interest and based on the highest educational level attained by the adolescent. The exact degree codes according to the Finnish Standard Classification of Education were obtained (Statistics Finland 2018). We classified two groups according to years of schooling: low (9 years) to middle (10–12 years) and high education (>12 years). Family SES Family SES was based on parents’education and categorised in the same way as that of adolescents’education. Data were obtained nearest to the year when the adolescent was aged 15 years and based on both mother’s and father’s education levels. If parents belonged to different categories, the highest was selected. If one parent had missing data, the available parent’s data were used. The minimum age of both parents was 30 years at the time their children participated in the surveys. Variables from the surveys Pubertal timing To obtain an indicator of pubertal timing (biological pathway), boys were asked about their age at first ejaculation, while girls were asked about their age at menarche. Classification of pubertal timing as early, average and late followed the groupings used by Koivusilta and Rimpel€ a (2004). In boys, the categories were chosen to be at age 12 or earlier (early), at 13 or 14 (average), and at 15 or later or if not occurred by the time of enquiry (late). In girls, the categories were at age 11 or earlier (early), at 12 or 13 (average), and at 14 or later or if not occurred by the time of enquiry (late). Reserve capacity Reserve capacity, spanning an underlying strong or weak construct, referred to a latent variable measured by nine observed variables in three distinct dimensions: 1. Perceived health dimension included three items: reported chronic disease, injury or disability that restricts daily activities (no/yes); a summary index of weekly perceived stress symptoms (stomach aches, tension or nervousness, irritability or outbursts of anger, trouble falling asleep or waking at night, headache, trembling of hands, feeling tired or weak, feeling dizzy) categorised as no symptoms, one symptom/week, 2–3/week, 4–8/week; and, self-rated health categorised as very good, average/ good or poor. 2. Health-promoting behaviour dimension included frequency of tooth brushing (several times a day, once a Pubertal timing School achievement Reserve capacity: Perceived health Health-promong behaviour Social support Figure 1. Conceptual model for the relationship of family SES with adult education level through adolescent pathways (biological, reserve capacity and school achievement). ANNALS OF HUMAN BIOLOGY 37 day, 1–5 times/week or less) and efficiency of physical activity. Efficiency of physical activity was measured by combining information from two variables: frequency of physical activity in leisure time and intensity of exercise (shortness of breath/sweating). This combination used the following categories: does not exercise; exercises with low/occasional efficiency; active efficient exerciser; very active efficient exerciser. 3. Social support dimension was measured using four items: nuclear family (living with both parents or not); ease of talking about troubling issues to father; to mother; and to friends (easy, difficult or very difficult). Those who did not have a father (5.2%), mother (1%) or friends (0.5%) were included in the ‘very difficult’category. School achievement For school achievement, adolescents were categorised based on self-assessment of their school performance as having: highest, 2nd highest, 2nd lowest or lowest academic achievement. The 14-year-old respondents (in comprehensive schools) were asked to assess whether their end-of-term school report was much better (highest), slightly better (2nd highest), average (2nd lowest), slightly poorer or much poorer (lowest) than the class average. For 16–18-year-olds, in addition to their self-assessment, school status (academic upper secondary school/vocational school/not attending school) was also used. Their achievement was classified as follows: highest (in academic upper secondary school with better performance); 2nd highest (in vocational school with better performance or academic upper secondary school with average performance); 2nd lowest (in vocational school with poor to average performance or high school with poor performance); and lowest (not at school). Statistical analysis We used confirmatory factor analysis (CFA) to estimate the underlying construct of ‘reserve capacity’and create a general, continuous latent variable from the nine measured variables: presence of chronic disease; perceived stress symptoms; self-rated health; physical activity; regular tooth brushing; nuclear family; talking to father; talking to mother; and talking to friends. We included covariances among variables within each dimension. We also fixed the value of the variance of the latent variable at one to freely estimate the factor loadings for all the variables. To analyse the mechanisms by which SES, puberty, reserve capacity and school achievement influence adult education level, we used structural equation modelling (SEM). This enabled the inclusion of latent effects and testing of multiple pathways simultaneously (Grace and Bollen 2005). SEM is composed of both a measurement model and a structural model. The measurement model is given by CFA, which shows how observed or measured variables relate to latent variables. The structural model describes the relationships among the variables, including the latent variables, through a set of regression equations (Muth en and Muth en 2012). In our study, the resulting estimates were probit coefficients, which are effects on a cumulative normal function of the probabilities that the response variable equals one (Muth en and Muth en 2012). We assigned a value of one to an outcome of low-to-middle adult education; thus, we predict this probability given a low family SES, delayed pubertal timing, weak reserve capacity and low school achievement. Models were fitted separately for each sex group and adjusted for both baseline age and age at follow-up. Since we wanted to assess if pubertal timing independently influenced the outcome, we initially tested for the effects of SES and puberty only (Model 1), then added reserve capacity (Model 2) and finally, school achievement (Model 3). All models were estimated using a robust weighted least squares estimator, under missing data theory which used all available data. In such analyses, missingness was allowed to be a function of the observed covariates, but not the observed outcome (Muth en and Muth en 2012). Fit of the CFA and full models (Model 3) were assessed using the root mean square error of approximation (RMSEA) and the comparative fit index (CFI). RMSEA values <0.08 and <0.06 imply acceptable and good fits, respectively. Similarly, CFI values >0.90 and >0.95 imply acceptable and good fits, respectively (Hooper et al. 2007). Mplus 7.11 was used for both CFA and SEM analyses. Results Sample characteristics Table 1 presents the descriptive characteristics of the adolescents in the sample according to the main variables. The proportions of those who had low-to-middle adult education largely exceeded those who had high education among boys (70.1%) and girls (59.3%). The majority of adolescents with low-to-middle adult education had parents with similarly attained education. Among those with available data, the average age of pubertal onset for boys was 13.1 ± 1.3 years, while for girls it was 12.6 ± 1.1 years. In terms of reserve capacity, there were higher proportions of adolescents with very good self-rated health, better health-promoting behaviours, presence of nuclear families and ease of communication with parents and friends among those with high adult education compared to those with low education. The same pattern was observed in the distribution of school achievement. CFA results Preliminary analyses showed that all factor loadings of the nine variables were statistically significant and the positive coefficients implied that each observed variable directly relates with latent reserve capacity (Table 2). Larger factor loadings reflect greater degrees of relationship with the latent variable. Among the nine variables, perceived stress symptoms and self-rated health, both of which are included in the perceived health dimension, contributed most to the measurement of the latent reserve capacity in both boys and 38 P. J. ACACIO-CLARO ET AL. Table 1. Characteristics of participants according to sex group and adult education level. Personal factors, family SES, reserve capacity and school achievement in adolescence Boys (n¼17,531) Girls (n¼20,345) Low/Middle High Low/Middle High n%n%n%n% Age at baseline (years) 14 4,182 34.0 1828 34.9 3,624 30.1 2951 35.6 16 4,412 35.9 1873 35.8 4,325 35.8 2972 35.9 18 3,701 30.1 1535 29.3 4,107 34.1 2366 28.5 Pubertal timing Early 2,731 22.2 1211 23.1 1,684 14.0 1213 14.6 Average 4,884 39.7 2449 46.8 7,709 63.9 5327 64.3 Late 3,067 25.0 1127 21.5 2,565 21.3 1714 20.7 No data 1,613 13.1 449 8.6 98 0.8 35 0.4 Parents’education High 1,227 10.0 1659 31.7 1,011 8.4 2178 26.3 Low/Middle 11,063 90.0 3577 68.3 11,039 91.6 6108 73.7 No data 5 0.0 0 0.0 6 0.0 3 0.0 Reserve capacity Perceived health dimension Chronic disease No 11,194 91.0 4796 91.6 10,759 89.2 7521 90.7 Yes 1,101 9.0 440 8.4 1,297 10.8 768 9.3 Perceived stress symptoms None 6,221 50.6 2647 50.6 3,636 30.2 2724 32.9 1/week 2,576 21.0 1119 21.4 2,657 22.0 1906 23.0 2–3/week 2,435 19.8 1117 21.3 3,535 29.3 2426 29.2 4–8/week 1,063 8.6 353 6.7 2,228 18.5 1233 14.9 Self-rated health Very good 4,502 36.6 2061 39.4 2,882 23.9 2525 30.5 Average/good 7,511 61.1 3080 58.8 8,833 73.3 5606 67.6 Poor 236 1.9 77 1.5 302 2.5 144 1.7 No data 46 0.4 18 0.3 39 0.3 14 0.2 Health-promoting behaviour dimension Physical activity Very active efficient exerciser 2,938 23.9 1677 32.0 1,824 15.1 1805 21.8 Active efficient exerciser 3,554 28.9 1735 33.2 3,242 26.9 2740 33.1 Occasional/low efficient exerciser 3,020 24.6 1094 20.9 3,966 32.9 2513 30.3 Does not exercise 2,740 22.3 719 13.7 3,000 24.9 1219 14.7 No data 43 0.3 11 0.2 24 0.2 12 0.1 Regular tooth brushing Several times/day 2,101 17.1 1584 30.2 5,644 46.8 4601 55.5 About once/day 5,967 48.5 2794 53.4 5,358 44.4 3309 39.9 About 1–5 times/week or less 4,151 33.8 846 16.2 1,031 8.6 360 4.4 No data 76 0.6 12 0.2 23 0.2 19 0.2 Social support dimension Nuclear family (with both parents) Yes 9,268 75.4 4471 85.4 8,577 71.1 6838 82.5 No 2,937 23.9 748 14.3 3,406 28.3 1419 17.1 No data 90 0.7 17 0.3 73 0.6 32 0.4 Talking about issues to father Easy 6,375 51.8 2763 52.8 4,003 33.2 3026 36.5 Difficult 3,762 30.6 1780 34.0 4,477 37.1 3435 41.4 Very difficult/No father 1,794 14.6 613 11.7 3,326 27.6 1762 21.3 No data 364 3.0 80 1.5 250 2.1 66 0.8 Talking about issues to mother Easy 8,454 68.7 3692 70.5 8,593 71.3 5984 72.2 Difficult 2,875 23.4 1246 23.8 2,622 21.7 1876 22.6 Very difficult/No mother 737 6.0 232 4.4 727 6.0 401 4.8 No data 229 1.9 66 1.3 114 1.0 28 0.4 Talking about issues to friends Easy 9,432 76.7 3945 75.4 10,392 90.7 7540 91.0 Difficult 2,093 17.0 1058 20.2 872 7.2 631 7.6 Very difficult/No friends 493 4.0 158 3.0 147 1.2 89 1.1 No data 277 2.3 75 1.4 105 0.9 29 0.3 School achievement Highest 1,026 8.3 1972 37.6 1,539 12.8 3611 43.6 2nd highest 2,987 24.3 2046 39.1 3,718 30.8 3204 38.6 2nd lowest 5,081 41.3 1014 19.4 4,453 36.9 1231 14.8 Lowest 3,009 24.5 182 3.5 2,212 18.4 221 2.7 No data 192 1.6 22 0.4 134 1.1 22 0.3 ANNALS OF HUMAN BIOLOGY 39 girls. The estimated coefficients for the covariances indicate the relationship of variables with one another. Table 2 showed that grouped variables had statistically significant covariances, implying that the observed variables were related within each dimension. RMSEA and CFI values signified good fit for our measurement models. Thus, the hypothesised reserve capacity framework in our study was consistent with observed data and provided support for our models in both boys and girls. The relationship of latent reserve capacity with other variables in the study is also illustrated in the bottom parts of Figures 2 and 3. SEM analyses To disentangle the influence of the biological pathway from those of other intermediate pathways, we assessed how effects of puberty on adult education vary when only family SES was considered (model 1), then reserve capacity (model 2) and school achievement (model 3) were sequentially added (Table 3). Results showed that delayed pubertal timing increased the probability of low adult education in boys (models 1 and 2), but lost statistical significance once the school achievement pathway was included. On the other hand, family SES consistently predicted the probability of adult education, regardless of adolescent pathways added into the models in both boys and girls. Model 3 is referred to as the full model and is illustrated in Figures 2 and 3. To simplify the model presentations, estimates relating to age variables and their covariances, along with covariances among adolescent pathways and among variables within the same dimension of reserve capacity, were not shown. Detailed results from SEM analyses of the full model depicting relationships among family SES, pubertal timing, school achievement and reserve capacity, while additionally controlling for age at baseline and at follow-up, showed that the models in both population groups (Figures 2 and 3) fit the data well based on the presented fit indices. The hypothesised pathways are described further below. Hypothesis 1: Family SES is related to pubertal timing, reserve capacity and school achievement This hypothesis was fully supported by the model in boys (Figure 2). Direct paths from family SES to the following factors: pubertal timing (b¼0.03), reserve capacity (b¼0.10) and 0.16 0.03 0.26 0.10 0.01 0.52 0.10 0.51 0.08 0.33 0.47 0.39 0.28 0.32 0.30 0.15 Figure 2. Boys: Structural equation model depicting relationships among family socioeconomic status (SES), pubertal timing, school achievement and reserve capacity in adolescence and adult education level (RMSEA ¼0.05; CFI ¼0.90). The values along the paths are standardised regression coefficients. Solid lines indicate statistically significant paths (p<0.001). Table 2. Results from confirmatory factor analyses (CFA) of reserve capacity model regressed on nine observed variables presented as standardised (b) coefficients. Boys Girls bp-value bp-value Observed variable Chronic disease 0.15 <0.001 0.07 0.003 Perceived stress symptoms 0.55 <0.001 0.46 <0.001 Self-rated health 0.70 <0.001 0.58 <0.001 Physical activity 0.32 <0.001 0.33 <0.001 Regular tooth brushing 0.17 <0.001 0.17 <0.001 Nuclear family 0.18 <0.001 0.26 <0.001 Talking about issues to father 0.38 <0.001 0.40 <0.001 Talking about issues to mother 0.34 <0.001 0.36 <0.001 Talking about issues to friends 0.22 <0.001 0.23 <0.001 Covariances Perceived health Chronic disease with Perceived stress symptoms 0.11 <0.001 0.23 <0.001 Self-rated health 0.18 <0.001 0.17 <0.001 Perceived stress symptoms with Self-rated health 0.08 0.002 0.08 <0.001 Health-promoting behaviour Physical activity with Regular tooth brushing 0.12 <0.001 0.10 <0.001 Social support Nuclear family with Talking about issues to father 0.33 <0.001 0.24 <0.001 Talking about issues to mother 0.10 <0.001 0.01 0.430 Talking about issues to friends 0.03 0.071 0.06 0.001 Talking about issues to father with Talking about issues to mother 0.55 <0.001 0.39 <0.001 Talking about issues to friends 0.24 <0.001 0.16 <0.001 Talking about issues to mother with Talking about issues to friends 0.28 <0.001 0.28 <0.001 Fit indices RMSEA 0.04 0.03 CFI 0.97 0.97 40 P. J. ACACIO-CLARO ET AL. school achievement (b¼0.26) were all statistically significant (p<0.001). The results in girls (Figure 3) partially supported this hypothesis, which showed only the pathways from family SES to reserve capacity (b¼0.13, p<0.001) and from family SES to school achievement (b¼0.25, p<0.001), as statistically significant. On the other hand, the relationship of family SES to girl’s pubertal timing differed from that found in boys. Among girls, a low family SES (b¼–0.02, p¼0.05) decreased the probability of delayed pubertal timing. Hypothesis 2: Pubertal timing and reserve capacity influence adult education level This hypothesis was also partially supported by the results. Figures 2 and 3illustrate statistically significant paths from reserve capacity to adult education in boys (b¼0.10, p<0.001) and girls (b¼0.12, p<0.001), respectively. While, the paths from pubertal timing to adult education were not statistically significant, a positive coefficient (b¼0.01) indicated a direct relationship between delayed pubertal timing and low-to-middle education in both boys and girls. Hypothesis 3: Family SES relates to adult education level directly and indirectly The results for boys (Figure 2) and girls (Figure 3) fully support this hypothesis as direct pathways from family SES to adult education in both boys (b¼0.16, p<0.001) and girls (b¼0.14, p<0.001) were statistically significant. Estimation of indirect paths in Table 4 showed that the effect of family SES on adult education is significantly mediated by reserve capacity (boys: b¼0.01; girls: b¼0.02; p<0.001) and school achievement (boys: b¼0.14; girls: b¼0.12; p<0.001) in the two groups. No mediation via pubertal timing was observed. How school achievement fits Direct paths from school achievement to adult education level, as shown in Figures 2 and 3, were statistically significant in both boys (b¼0.52; p<0.001) and girls (b¼0.48; p<0.001), respectively. We also found statistically significant covariances among pubertal timing, reserve capacity and school achievement in boys, while, in girls, similar statistically 0.14 -0.02 0.25 0.13 0.01 0.48 0.12 0.48 0.07 0.31 0.44 0.30 0.38 0.35 0.29 0.17 Figure 3. Girls: Structural equation model depicting relationships among family socioeconomic status (SES), pubertal timing, school achievement and reserve capacity in adolescence and adult education level (RMSEA ¼0.04; CFI ¼0.91). The values along the paths are standardised regression coefficients. Solid lines indicate statistically significant paths (p<0.001). Table 3. Direct effects of family SES and biological pathway on adult education level in a structural equation model presented as standardised (b) coefficients. Direct effects based on different models Boys Fit indices Girls Fit indices SES Puberty RMSEA/CFI SES Puberty RMSEA/CFI Model 1 a 0.300.03—0.280.00 — Model 2 b 0.290.050.05/0.89 0.250.030.04/0.90 Model 3 c 0.160.01 0.05/0.90 0.140.01 0.04/0.91 Note: All models were adjusted for ages at baseline and follow-up. Statistically significant at p<0.5. a Model with family SES and puberty. b Model 1 plus reserve capacity. c Model 2 plus school achievement. Table 4. Estimated indirect effects of family SES through adolescent pathways and the covariances among these pathways in the final structural equation model presented as standardised (b) coefficients. Indirect effect of family SES through Boys Girls Pubertal timing 0.00 0.00 School achievement 0.140.12 Reserve capacity 0.010.02 Covariance between pathways Boys Girls Pubertal timing and reserve capacity 0.110.12 Pubertal timing and school achievement 0.050.01 Reserve capacity and school achievement 0.350.37 Statistically significant at p<0.001. ANNALS OF HUMAN BIOLOGY 41 significant covariances existed, except between pubertal timing and school achievement (Table 4). The covariances indicate the direction of the relationship between the variables. As shown in Table 4, pubertal timing had a negative relationship with reserve capacity, but a positive relationship with school achievement. In our study, this means that delayed pubertal timing was related to better reserve capacity in both boys and girls, but lower school achievement in boys. On the other hand, a weak reserve capacity was related to low school achievement. Discussion Summary and interpretation of results We investigated the relationships among family SES, the intermediate pathways in adolescence and adult education. We found that family SES directly predicted the measured adolescent pathways (except biological pathway in girls) and adult education. Reserve capacity and school achievement directly influenced adult education and mediated the relationship between family SES and adult education. Although we did not find statistical significance for the path between pubertal timing and adult education, unadjusted results suggested that delayed pubertal timing might be a risk for having low-to-middle adult education in both boys and girls. Additionally, we found that pubertal timing, reserve capacity and school achievement were inter-related, providing empirical evidence on how mechanisms in adolescence work to influence educational outcomes. Clearly, our study showed that family SES predicted the adolescents’educational outcomes, directly and indirectly, via pathways of reserve capacity and school achievement. The significant effect of SES on adult education implies that educational inequalities existed in our setting. This is comparable with analyses of more current data attributed to the rising income inequality observed in the region within recent years (OECD 2018). On the other hand, research also showed that higher levels of social mobility occur in welfare Scandinavian societies such as Finland, where the economic inequality gap is narrower than in many other countries (OECD 2018). Indeed, we observed greater upward social mobility where children born into low SES families ended up in higher SES than their parents (Table 1). The revealed indirect pathways of SES supported previous knowledge that SES affects life-course developments such as psychosocial, behavioural and cognitive functioning (Kroenke 2008; Conger et al. 2010). We can infer that the parents’SES influenced the reserve capacity and school achievement of the adolescents probably through family dynamics such as family stress processes and parenting practices including cognitive stimulation and parental investments for education (Conger et al. 2010; Martin et al. 2010). The adolescents with stronger reserve capacity and higher school achievement than their peers may have utilised their cognitive abilities, psychosocial and behavioural resources to cope with academic transitions and attain higher education and, consequently, better SES in the future. As one study showed, the pursuit of higher education, controlling for social origin, was dependent on academic motivation and abilities and subjective expectations and evaluations of return of investments on higher education (Becker and Hecken 2009). Like Obeidallah et al. (2000), we did not observe a statistically significant direct effect of family SES on menarche. On the other hand, we found that a low family SES increased the probability of delayed pubertal timing in boys. Our results supported previous findings which had documented inverse associations between SES and pubertal onset within populations (de Muinich Keizer and Mul 2001; Parent et al. 2003). Living in low socioeconomic conditions might delay puberty because of a higher likelihood of malnutrition, acute or chronic illnesses and the presence of other adverse physical or psychological conditions compared to those living in privileged environments (Parent et al. 2003). In contrast, recent evidence revealed that low family SES markedly increased rates of early puberty in both boys and girls (Downing and Bellis 2009; Sun et al. 2017), possibly through interactions with biological systems regulating pubertal timing (Sun et al. 2017) or other risk factors such as having a higher body mass index (BMI) or being overweight (Downing and Bellis 2009; James-Todd et al. 2010) and experiencing stressful life events (James-Todd et al. 2010). However, a meta-analysis of studies among males found no significant association between family SES and pubertal timing (Xu et al. 2018). Since there is limited research on determinants of pubertal onset among boys, the processes influencing male pubertal development were much less understood (Graber 2013). We conclude that the inconsistent relationship of family SES with pubertal timing probably reflected inherent differences in study populations such as ethnic and geographic variations, gender and genetic predisposition and changes in underlying mechanisms influenced by SES to activate puberty such as intrauterine conditions, health, nutrition, stress and environmental exposures (Parent et al. 2003). Other methodological issues including differences in study designs and measurement of SES and pubertal timing indicators (Xu et al. 2018) might have contributed to this inconsistency. In our study, low family SES increased the probability of having weak reserve capacity brought about by poor perceived health, health-promoting behaviour and social support. Our findings are congruent with previous evidence, albeit reserve capacity was measured using purely psychosocial resources (Kroenke 2008; Matthews and Gallo 2011). According to Gallo and Matthews (2003), low-SES individuals have weaker reserve capacity due to frequent exposure to situations requiring use of their psychosocial resources and their environments inhibit them from developing and replenishing these resources ‘to be kept in reserve’. While reserve capacity was initially conceptualised as a potential mediating pathway in SES-health inequalities (Gallo et al. 2009), we have shown that it also served as a pathway connecting one’s family SES to future adult education. Indeed, an indirect effect of family SES through this pathway was statistically significant in both boys and girls. We believe that dealing with school transitions, along with puberty during adolescence, constantly requires the use of one’s reserve capacity. 42 P. J. ACACIO-CLARO ET AL.